-
1
Construction of a gene knockout cassette for leucosporidium antarcticum
Published 2011“…Hence, there is a need to construct a knock-out system for L. antarcticum. The gene knockout constructs were built .by fusing ,an .antibiotic (Genetidn-E418) resistance .gene, KanMX used for gene knock out in Saccharomyces cerevisiae to a series of L. antarcticum gene's promoters. …”
Get full text
Get full text
Thesis -
2
The cross-sectional relations of Covid-19 fear and stress to psychological distress among frontline healthcare workers in Selangor, Malaysia
Published 2021“…Previous pandemics have demonstrated short and long-term impacts on healthcare worker's mental health, causing knock-on effects on patient care and professional functioning. …”
Get full text
Get full text
Article -
3
A novel Pseudorabies virus vaccine developed using HDR-CRISPR/Cas9 induces strong humoral and cellular immune response in mice
Published 2022“…Our study showed that the four-sgRNA targeting system appeared to have higher knock-in efficiency for PRVs editing. The replication of the recombinant PRVs were slightly lower than that of the parental strain, but they appeared to have similar properties in terms of growth curves and plaque morphology. …”
Get full text
Get full text
Article -
4
Assessment of the inhibitory mechanism of action for a yeast cell-based screening system targeting glycogen synthase kinase-3ß (GSK-3ß)
Published 2017“…Methodology: In this study, the GSK-3 homologues in yeast (MCK1, MDS1, MRK1 and YOL128C) were knocked out and inserted with mammalian GSK-3β. In order to determine the inhibitory mechanism, known GSK-3β inhibitors were tested and evaluated. …”
Get full text
Article -
5
GSK3 beta-inhibitory activity in microfungal isolated from Sabah rainforest soils
Published 2017“…The homologous genes of GSK-3 in the yeast (MCK1, MDS1, MRK1, and YOL128C) were knocked out and inserted with mammalian GSK-3β to overcome temperature-sensitive phenotype of the mutant at 37oC thus created a yeast strain that is capable to grow at both 25oC and 37oC. …”
Get full text
Get full text
Thesis