Mutagenesis Of Microbial Transglutaminase For QTAG-KTAG Conjugation by Chan, Soo Khim

“…However, mTGase has low substrate specificity due to its wide active site cleft which impeding its diverse applications in biotechnological use. Therefore, mutagenesis of mTGase to obtain substrate specific enzyme is highly desirable for site-specific conjugations.…”
Get full text

Identification of important residues for catalysis and substrate specificity in human choline kinase by site directed mutagenesis by Wong Mun, Teng

Get full text

Site directed mutagenesis of miRNA binding site on the 3′-UTR of choline kinase alpha gene by Bnisallama, Faisal Fayez Saffah

“…In vitro work, after mutagenesis step, the PCR products were sent for sequencing, however the results were not satisfactory.…”
Get full text

Site directed mutagenesis on highly conserved amino acid residues of choline kinase gene from neisseria meningitidis by Xiang, Cheong Jing

“…The present study aims to identify and modify important amino acid residues for NmCK catalysis that could be targeted for inhibition by performing two-step PCR site directed mutagenesis. Two aspartates (D153 and D170) in the CK motif of NmCK have been identified as highly conserved and chosen for mutagenesis into alanine. …”
Get full text

Cloning and expression of CYP2D6*1 and CYP2D6*10 and its application in vitro drug-herbs interaction studies by Lee, Wee Leng

“…Recombinant CYP2D6*10 was generated using site-directed mutagenesis and expressed as CYP2D6* 1. …”
Get full text

In Vitro Selection Of Dendrobium Sonia-28 Protocorm-Like Bodies Against Fusarium Proliferatum by Dehgahi, Raheleh

“…Untuk mengatasi masalah ini, salah satu strategi yang sedang diusahakan adalah dengan penghasilan orkid mutan baru. Mutagenesis secara in vitro dengan cara kultur turasan Fusarium proliferatum (CF), asid fusarik (FA) dan sinaran gama boleh digunakan untuk menghasilkan mutan yang lebih baik dari segi ekonomi. …”
Get full text

Molecular Dynamics Simulation Of Polyhydroxyalkanoate Synthase From Chromobacterium Sp. Usm2 by Annamalai, Nithyanan

“…Based on the result obtain via site-directed mutagenesis, it has been postulated that mutation of residue Arg409Ala and Arg490Ala of the PhaCCs disrupt the formation of PhaCCs dimer. …”
Get full text

Production of highly immunogenic/ hydrophilic regions of naiha gene using assembly pcr by Shobana Devi, Murugiah

“…PCR-based site directed mutagenesis technique must be performed to repair those errors. …”
Get full text

Identification of amino acid responsible for the binding of manganese ion in entamoeba histolytica choline kinase by Phang, Simon Hoi Theng

“…Gly-45 was replaced by alanine using PCR site directed mutagenesis. Mutant EhCK-G45A was cloned into pGEX-RB vector, expressed and purified. …”
Get full text

In Silico Design And Constant-Ph Molecular Dynamics Study Of Human Igg1 Fc At Ph6.0 And Ph 7.5 by Lim, Yee Ying

“…To obtain alternative Fc design with better binding properties for FcRn, pH 6.0 single trajectory MutYTE-FcRn representing complex was extracted by MMTSB toolset clustering followed by SAAMBE server single point mutations imposed on the MutYTE Fc of the complex. Mutagenesis result suggested four Fc positions with improved binding energy (ΔG) for FcRn. …”
Get full text

Effects of CpG islands DNA methylation on the human choline kinase alpha promoter activity by Sa'dom, Siti Aisyah Faten Mohamed

“…Then, a series of CpG island deletion mutants were constructed using PCR site-directed mutagenesis method and cloned into pGL4.10 vector and studied in human breast adenocarcinoma, MCF-7 cells. …”
Get full text