Mutation of cyclodextrin glycosyltransferase by domain swapping and site-directed mutagenesis by Kong, Wan Looi

“…There has been numerous studies on mutagenesis of CGTase. In this study, a mutant CGTase H43T/Y87F which was previously constructed was further mutated by domain swapping and site-directed mutagenesis. …”

Uv induced mutagenesis elevates the production of laccase in enterobacter cloacae by Deepa, T., Gangwane, A. K., Sayyed, R. Z., Sapna, Sapna, El Enshasy, H. A., Zaidel, D. N. A

“…UV mutagenesis of a wild strain of E. cloacae resulted in 3.09 folds further improvement in the production. …”

Rational mutagenesis of cyclodextrin glucanotransferase at the calcium binding regions for enhancement of thermostability by Goh, Poh Hong, Md. Illias, Rosli, Goh, Kian Mau

“…The calcium binding regions that are known for thermostability function were subjected to site-directed mutagenesis in this study. The starting gene-protein is a variant of CGTase Bacillus sp. …”
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Random Mutagenesis approach to increase catalytic activity of recombinant amylase from Anoxybacillus by Ng, Han Chyi @ Pearly Ng

Manipulation of the conformation and enzymatic properties of T1 Lipase by site-directed mutagenesis of the protein core by Abdul Wahab, Roswanira, Basri, Mahiran, Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Abdul Rahman, Mohd. Basyaruddin, Leow, Thean Chor

Genomic analysis of a riboflavin- overproducing ashbya gossypii mutant isolated by disparity mutagenesis by Kato, T., Azegami, J., Yokomori, A., Dohra, H., El Enshasy, H. A., Park, E. Y.

“…In this study, to investigate the change in metabolism of a riboflavin-overproducing mutant, namely, the W122032 strain (MT strain) that was isolated by disparity mutagenesis, genomic analysis was carried out. Results: In the genomic analysis, 33 homozygous and 1377 heterozygous mutations in the coding sequences of the genome of MT strain were detected. …”
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In silico site-directed mutagenesis of Acinetobacter Haemolyticus Lipase KV1 for improved alkaline stability by Khairul Anuar, Nurul Fatin Syamimi

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Protein engineering of alpha-amylase from anoxbacillus sp, SK3-4 with saturation mutagenesis by Olya, Mohammad Emad

Identification of genes involved in the 4-aminobenzenesulfonate degradation pathway of hydrogenophaga sp PBC via transposon mutagenesis by Han, Ming Gan, Ibrahim, Zaharah, Shahir, Shafinaz, Yahya, Adibah

“…PBC were identified using transposon mutagenesis. The screening of 10000 mutants for incomplete 4-ABS biotransformation identified four mutants with single transposon insertion. …”
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Identification of genes involved in the 4-aminobenzenesulfote degradation pathway of hyrogenophaga sp.PBC via transposon mutagenesis by Han, Ming Gan, Ibrahim, Zaharah, Shahir, Shafinaz, Yahya, Adibah

“…PBC were identified using transposon mutagenesis. The screening of 10 000 mutants for incomplete 4-ABS biotransformation identified four mutants with single transposon insertion. …”

Increasing thermostability of cyclodextrin glucanotransferase h43t/y87f using site-directed mutagenesis by Goh, Kian Mau

Enhanced secretory production of hemolysin-mediated cyclodextrin glucanotransferase in Escherichia coli by random mutagenesis of the ABC transporter system by Low, Kheng Oon, Mahadi, Nor Muhammad, Abdul Rahim, Raha, Rabu, Amir, Abu Bakar, Farah Diba, Abdul Murad, Abdul Munir, Md. Illias, Rosli

Optimization of a heterologous signal peptide by site-directed mutagenesis for improved secretion of recombinant proteins in escherichia coli by Jonet, M. A., Mahadi, N. M., Murad, A. M., Rabu, A., Bakar, F. D., Rahim, R. A., Low, K. O., Illias, R. M.

“…Eight mutant SPs were constructed using site-directed mutagenesis to improve the secretion of recombinant CGTase. …”

Site-saturation mutagenesis of mutant L-asparaginase II signal peptide hydrophobic region for improved excretion of cyclodextrin glucanotransferase by Ismail, A., Illias, R. M.

“…In this study, modification of the hydrophobic region of the N1R3 signal peptide using site-saturation mutagenesis improved the excretion of CGTase. Signal peptide mutants designated M9F, V10L and A15Y enhanced the excretion of CGTase three-fold and demonstrated two-fold higher secretion rate than the wild type. …”

Improvement and screening of astaxanthin producing mutants of newly isolated Coelastrum sp. using ethyl methane sulfonate induced mutagenesis technique by Tharek, Ameerah, Yahya, Adibah, Md. Salleh, Madihah, Jamaluddin, Haryati, Yoshizaki, Shinji, Hara, Hirofumi, Iwamoto, Koji, Suzuki, Iwane, Mohamad, Shaza Eva

“…To increase the productivity of astaxanthin in microalgae, random mutagenesis has been extensively used to improve the yield of valuable substances. …”
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Random mutagenesis of NS1 protein of influenza a H1N1 and docking of RNA aptamers to wild type and mutant NS1 proteins by Chelliah, Kumutha

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Use of megaprimer and overlapping extension pcr (OE-PCR) to mutagenize and enhance cyclodextrin glucosyltransferase (CGTASE) function by Goh, Kian Mau, Liew, Kok Jun, Chai, Kian Piaw, Md. Illias, Rosli

“…In this chapter, we describe two protocols for performing site-directed mutagenesis of any protein-coding sequence, namely, megaprimer PCR and overlapping extension PCR (OE-PCR). …”

A review on non-stereospecific haloalkanoic acid dehalogenases by Tengku Abdul Hamid, Tengku Haziyamin, Abdul Hamid, Azzmer Azzar, Huyop, Fahrul

“…A structural prediction, sequence-homology modeling and a site-directed mutagenesis study of DehE might help achieve this goal.…”

In silico analysis and truncation studies of the c terminal alpha amylase from anoxybacillus species by Sea, Lillian Shun Yi

“…The present study involved in silico analyses and protein mutagenesis of two C-terminal truncated variants, namely mutant ?…”
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Facile modulation of enantioselectivity of thermophilic Geobacillus zalihae lipase by regulating hydrophobicity of its Q114 oxyanion by Abdul Wahab, R., Basri, M., Raja Abdul Rahman, R. N. Z., Salleh, A. B., Abdul Rahman, M. B., Leow, T. C.

“…Site-directed mutagenesis of the oxyanion-containing amino acid Q114 in the recombinant thermophilic T1 lipase previously isolated from Geobacillus zalihae was performed to elucidate its role in the enzyme's enantioselectivity and reactivity. …”