DNA replication licensing in somatic and germ cells. by Eward, K, Obermann, E, Shreeram, S, Loddo, M, Fanshawe, T, Williams, C, Jung, H, Prevost, A, Blow, J, Stoeber, K, Williams, G

“…We have investigated regulation of the origin licensing factors Cdc6, Cdt1, Mcm2 and Geminin in human somatic and germ cells. Cdc6 and Cdt1 play an essential role in DNA replication initiation by loading the Mcm2-7 complex, which is required for unwinding the DNA helix, onto chromosomal origins. …”

One PRDM is not enough for germ cell development. by Bikoff, E, Robertson, E

“…Developmentally regulated expression of the transcriptional repressor Prdm1 (Blimp1) in the early mammalian embryo controls global epigenetic changes required for specification of primordial germ cells. A new study demonstrates that a close family member, Prdm14, similarly activated in response to Bmp and Smad signals, also has an essential role during establishment of the germ cell lineage. © 2008 Nature Publishing Group.…”

DNA replication licensing in somatic and germ cells by Eward, K, Obermann, E, Shreeram, S, Loddo, M, Fanshawe, T, Williams, C, Jung, H, Prevost, A, Blow, J, Stoeber, K, Williams, G

“…We have investigated regulation of the origin licensing factors Cdc6, Cdt1, Mcm2 and Geminin in human somatic and germ cells. Cdc6 and Cdt1 play an essential role in DNA replication initiation by loading the Mcm2-7 complex, which is required for unwinding the DNA helix, onto chromosomal origins. …”

Investigation into techniques to isolate and develop human germ cells in vitro by Papadopoulou, E

“…Analyses revealed that age affected the efficacy of dissociation of frozen/thawed ovarian cortical tissue. To detect germ cells in a single cell suspension of ovarian cells, a method was developed for flow cytometry using a monoclonal antibody that recognises the (cytoplasmic) N-terminus region of DDX4. …”

Angiogenesis and lymphangiogenesis in stage 1 germ cell tumours of the testis. by Jones, A, Fujiyama, C, Turner, K, Fuggle, S, Cranston, D, Turley, H, Valtola, R, Bicknell, R, Harris, A

“…OBJECTIVE: To determine whether angiogenesis can be used as an additional prognostic indicator in patients with stage 1 germ cell tumours of the testis. PATIENTS AND METHODS: Paraffin sections were assessed immunohistochemically from 51 patients with clinical stage 1 germ cell tumours of the testis (28 seminoma, 23 teratoma) treated by orchidectomy and surveillance only. …”

VITRONECTIN EXPRESSION IN HUMAN GERM-CELL TUMORS AND NORMAL MOUSE DEVELOPMENT by Cooper, S, Bennett, W, Roach, S, Pera, M

Digital pathology transformation in a supraregional germ cell tumour network by Colling, R, Protheroe, A, Sullivan, M, Macpherson, R, Tuthill, M, Redgwell, J, Traill, Z, Molyneux, A, Johnson, E, Abdullah, N, Taibi, A, Mercer, N, Haynes, HR, Sackville, A, Craft, J, Reis, J, Rees, G, Soares, M, Roberts, ISD, Siiankoski, D, Hemsworth, H, Roskell, D, Roberts-Gant, S, White, K, Rittscher, J, Davies, J, Browning, L, Verrill, C

“…<strong>Background: </strong>In this article we share our experience of creating a digital pathology (DP) supraregional germ cell tumour service, including full digitisation of the central laboratory. …”

Stochastic specification of primordial germ cells from mesoderm precursors in axolotl embryos. by Chatfield, J, O'Reilly, M, Bachvarova, R, Ferjentsik, Z, Redwood, C, Walmsley, M, Patient, R, Loose, M, Johnson, A

“…For example, in Xenopus and zebrafish embryos primordial germ cells (PGCs) are specified by germ plasm that is inherited from the egg; in mice, Blimp1 expression in the epiblast mediates the commitment of cells to the germ line. …”

Germ cell tumours of the testicle as a model of MHC influence on human malignancy. by Oliver, RT, Stephenson, C, Parkinson, M, Forman, D, Atkinson, A, Bodmer, J, Bodmer, W

A rare case of large mediastinal germ cell tumor detected by echocardiography. by Karas, S, Parissis, J, Antoniades, C, Loulias, A

“…We describe a rare case of a large mediastinal germ cell tumor detected by modified views of transthoracic echocardiography. …”

Deep learning for detecting tumour infiltrating lymphocytes in testicular germ cell tumors by Linder, N, Taylor, J, Colling, R, Pell, R, Alveyn, E, Joseph, J, Protheroe, A, Lundin, M, Lundin, J, Verrill, C

“…<strong>Aims</strong> To evaluate if a deep learning algorithm can be trained to identify tumour-infiltrating lymphocytes (TILs) in tissue samples of testicular germ cell tumours and to assess whether the TIL counts correlate with relapse status of the patient. …”

Germ cell tumours of the testicle. A model for MHC influence on human malignancy. by Oliver, RT, Stephenson, C, Parkinson, M, Forman, D, Atkinson, A, Bodmer, J, Bodmer, W

Up-regulation of the lymphatic marker podoplanin in human squamous cell carcinomas and germ cell tumors by Schacht, V, Dadras, S, Johnson, L, Jackson, D, Hong, Y, Detmar, M

Regulative germ cell specification in axolotl embryos: a primitive trait conserved in the mammalian lineage. by Johnson, A, Crother, B, White, M, Patient, R, Bachvarova, R, Drum, M, Masi, T

“…How germ cells are specified in the embryos of animals has been a mystery for decades. …”

Identification of genomic changes associated with cisplatin resistance in testicular germ cell tumor cell lines. by Noel, E, Perry, J, Chaplin, T, Mao, X, Cazier, J, Joel, S, Oliver, RT, Young, B, Lu, Y

“…Since the introduction of cisplatin into the clinic, the treatment of patients with a variety of solid tumors including testicular germ cell tumors, ovarian and lung cancers, has dramatically improved. …”

Genetic dissection of Nodal and Bmp signalling requirements during primordial germ cell development in mouse by Senft, AD, Bikoff, EK, Robertson, EJ, Costello, I

“…Thus balanced Nodal/Bmp signalling cues regulate germ cell versus somatic cell fate decisions in the early posterior epiblast.…”

Suprasellar germ cell tumours: specific problems and the evolution of optimal management with a combined chemoradiotherapy regimen. by Janmohamed, S, Grossman, AB, Metcalfe, K, Lowe, D, Wood, D, Chew, S, Monson, J, Besser, G, Plowman, P

“…OBJECTIVE: Suprasellar germ cell tumours are rare, and there are few series of patients outlining the problems in diagnosis and management, and providing clear guidelines for optimal therapy. …”

Nut directs p300-dependent, genome-wide H4 hyperacetylation in male germ cells by Shiota, H, Barral, S, Buchou, T, Tan, M, Couté, Y, Charbonnier, G, Reynoird, N, Boussouar, F, Gérard, M, Zhu, M, Bargier, L, Puthier, D, Chuffart, F, Bourova-Flin, E, Picaud, S, Filippakopoulos, P, Goudarzi, A, Ibrahim, Z, Panne, D, Rousseaux, S, Zhao, Y, Khochbin, S

“…Shiota et al. show the oncogenic factor Nut is expressed in post-meiotic male germ cells, where it recruits p300 and/or CBP and enhances histone H4K5 and H4K8 acetylation, leading to histone-to-protamine replacement.…”

Paternal age effect mutations in germ cell development: pathological correlates in normal testis and testicular tumours by Lim, J

“…Recently, spermatocytic seminoma (SS; a rare testicular germ cell tumour that occurs mainly in older men) has emerged as a key link between the processes of somatic and germline mutation (Goriely <em>et al, Nat Genet.…”

Development of a best-practice clinical guideline for the use of bleomycin in the treatment of germ cell tumours in the UK by Watson, R, De La Peña, H, Tsakok, M, Joseph, J, Stoneham, S, Shamash, J, Joffe, J, Mazhar, D, Traill, Z, Ho, L, Brand, S, Protheroe, A

“…Bleomycin, a cytotoxic chemotherapy agent, forms a key component of curative regimens for lymphoma and germ cell tumours. It can be associated with severe toxicity, long-term complications and even death in extreme cases. …”