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Reverse transcription-polymerase chain reaction (RT-PCR) based detection and economic impact of foot-and-mouth disease in District Faisalabad, Pakistan during the year 2015
Published 2017-06-01“…Sampling was done from different outbreaks initially on the basis of clinical signs and later reverse transcriptase-polymerase chain reaction (RT-PCR) was employed for the conformation of FMDV genome. …”
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First serodetection and molecular phylogenetic documentation of Coxiella burnetii isolates from female camels in Wasit governorate, Iraq
Published 2021-12-01Subjects: Get full text
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Molecular investigation of feline calicivirus in cats in Mosul city, Iraq
Published 2022-06-01Subjects: Get full text
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ISOLATION, IDENTIFICATION AND BIOCONTROL OF SALMONELLA TYPHIMURIUM IN KARIESH CHEESE BY BACTEIOPHAGE
Published 2018-05-01Subjects: “…salmonella typhimurium identification by polymerase chain reaction (pcr)…”
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DETECTION FOR MECA GENE IN EGYPTIAN CLINICAL Staphylococcus aureus SAMPLES AND STUDY THEIR GENOTOXIC ACTIVITY
Published 2019-03-01Subjects: Get full text
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العزل والوصف الجزيئي لجراثيم البروسيلة المالطية المسببة للإجهاض عند الماعز في المنطقة الوسطى من سوريا والتفريق بين الذراري الحقلية والذراري اللقاحية المعزولة في الدراسة...
Published 2013-02-01“…The results of bacterial isolation and polymerase chain reaction reveal that the prevalence of caprine abortion caused by Brucella melitensis was 53.4%. …”
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العزل والوصف الجزيئي لجراثيم البروسيلة المالطية المسببة للإجهاض عند الماعز في المنطقة الوسطى من سوريا والتفريق بين الذراري الحقلية والذراري اللقاحية المعزولة في الدراسة...
Published 2013-02-01“…The results of bacterial isolation and polymerase chain reaction reveal that the prevalence of caprine abortion caused by Brucella melitensis was 53.4%. …”
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Prevalence of ovine theileriosis in Mosul city, Iraq
Published 2023-01-01“…The present study aimed to determine the prevalence of ovine theileriosis (OT) in sheep in Mosul city, Iraq using microscopic examination (ME) of the blood smears stained with MGG- Quick stain and conventional polymerase chain reaction technique (c-PCR) to compare between c-PCR technique and ME as techniques for the diagnosis of disease, and to investigate the pattern and type of infections based on multiplex polymerase chain reaction technique (m-PCR). …”
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Identification of a Methylation Pattern in the SNRPN Gene Promoter and its Association with Semen Abnormality Among Iraqi Males
Published 2023-02-01“…The targeted region was amplified by polymerase chain reaction (PCR) technique. In addition, the SNRPN gene's promoter methylation was qualitatively detected using Real time polymerase chain reaction (qPCR) utilizing two sets of primers: methylated and un-methylated. …”
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التحري عن إيجابية Anti-HBc عند مرضى التحال الدموي المصابين بالتهاب الكبد C
Published 2013-01-01“…واستخدمت طريقة الإليزا (ELISA) لدراسة انتشار الأضداد اللبية عند مرضى الدراسة، واستخدمت طريقة تفاعل البوليميراز التسلسلي بالزمن الحقيقيPolymerase Chain Reaction - real time (PCR-RT) عند المرضى إيجابيي الأضداد اللبية. …”
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التحري عن إيجابية Anti-HBc عند مرضى التحال الدموي المصابين بالتهاب الكبد C
Published 2013-01-01“…واستخدمت طريقة الإليزا (ELISA) لدراسة انتشار الأضداد اللبية عند مرضى الدراسة، واستخدمت طريقة تفاعل البوليميراز التسلسلي بالزمن الحقيقيPolymerase Chain Reaction - real time (PCR-RT) عند المرضى إيجابيي الأضداد اللبية. …”
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Identification of beef using restriction fragment length polymorphism–
Published 2009-01-01“…To differentiate the beef from other types of meat consumed by human, DNA markers based on polymerase chain reaction restriction fragment length polymorphism technique is performed by using universal primers designed on mitochondrial cytochrome b gene to obtain amplified band 359 bp, then digested with some of restriction enzymes like Tru91, RsaI, Hinf I, Hae III, Alu I, Taq I, Mob I. …”
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Study of Phenotypic and Genotypic Factors of Staphylococcus aureus Clinical Local Isolates
Published 2022-12-01“…The isolates of Staphylococcus aureus were isolated from patients with various infections in hospitals, the isolates were identified and accurately diagnosed by phenotypic examination and biochemical tests, as well Vitek-2, and then genetic detection and diagnosis of many of the pathogenic factors associated with Staphylococcus aureus using conventional polymerase chain reaction (PCR) and testing for association by antibiotic resistance and production of some toxins by Staphylococcus aureus. …”
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BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF SOME YEAST ISOLATES
Published 2007-09-01“…Sixteen yeast isolates from various sources in-cluding molasses, foodstuffs, concentrated juices and soils were identified using simplified API 20 C AUX yeast identification system and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The isolates were found to belong to 11 species; Candida famata, C. glabra-ta, C. krusei / inconspicua, C. norvegensis, C. col-liculosa, Cryptococcus humicolus, Rhodotorula mucilaginosa, Kloeckera spp., Geotrichum capita-tum and Saccharomyces cerevisiae. …”
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Phylogenetic study of Theileria lestoquardi based on 18SrRNA gene Isolated from sheep in the middle region of Iraq
Published 2016-12-01“…The multiple sequence alignment analysis and neighbor joining phylogenetic tree analysis were performed by using ClustalW multiple sequence alignment online based analysis of 1098bp 18S rRNA gene was amplified by polymerase chain reaction. Phylogenetic analysis results of these gene sequences revealed that T. lestoquardi local isolates were closely related to T. lestoquardi Iran isolate (JQ917458.1) and two Iraq Kurdistan isolates (KC778786.1 and KC778785.1) more than other countries. …”
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Molecular study of Oestrus ovis larvae infesting in sheep in Baghdad city, Iraq
Published 2022-12-01“…The aim of this study is to detect the 28S (rRNA) gene sequences of <em>Oestrus ovis</em> larvae by conventional polymerase chain reaction and to compare their genetic relatedness utilizing phylogenetic analysis. …”
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Sequencing and phylogenetic analysis of Entamoeba histolytica in cattle in Al-Diwaniyah governorate, Iraq
Published 2023-07-01“…Microscopy and polymerase chain reaction (PCR) were used to examine the samples. …”
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ال طفرة جينة NUDT15 R139C تزيد من خطورة تطور سمية محرضة بمركبات mercaptopurine عند مريضة سورية: تقرير حالة
Published 2022-10-01“…وتم التحري عن نمط جينة NUDT15 باستخدام تقنية PCR Polymerase chain reaction. النتائج: أظهرت النتائج أن المريضة حاملة للنمط المتجانس homozygous carrier من الشكل الطافر T (415C>T) لجينة NUDT15 ذات الرقم rs116855232. …”
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Traditional, histopathological and molecular diagnosis of sarcocytosis in slaughtered sheep in Al-Diwaniyah province, Iraq
Published 2023-10-01“…The samples were examined for detection of the Sarcocystic bradyzoites using microscopic, histological, 18S rRNA gene-based polymerase chain reaction (PCR) and PCR-product-dependent gene sequencing methods. …”
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Nc-5 gene-dependent molecular identification and phylogenetic investigation of Neospora caninum in sheep
Published 2024-04-01“…After extracting the DNA, the samples were subjected to molecular techniques, polymerase chain reaction (PCR), and partial gene sequencing accompanied by estimating the phylogenetic status of the N. caninum coccidial microorganism. …”
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