Simulation Analysis of Knocking Noise of Two Speed Automatic Transmission in Electric Vehicle by Yonggan Lei, Yong Chen, Dawei Zheng, Guangxin Li, Changyin Wei, Libin Zang, Hangcong Lu, Xiaozhe Lin

Subjects: “…Automatic transmissison;Knocking noise;Multibody dynamics;Retarding torque…”
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ESTABLISHMENT OF A RAPID SCREENING METHOD FOR IN VITRO SITE-DIRECTED MUTANTS OR CRISPR/Cas9-MEDIA-TED KNOCK-IN MUTANTS by SONG Chunlin, ZHANG Yuqing, ZHAI Yujing, ZHANG Gang, WANG Ying

Subjects: “…polymerase chain reaction|mutagenesis, site-directed|mutation|crispr/cas9|gene knock-in techniques|mutagenicity tests…”
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Preparation of B2M and PD-1 Efficiently Double Genes Knock-out Primary Human T Lymphocytes by Cas9 RNP Technology by XU Ya, FU Yang, ZHU Shicong, ZHANG Bicheng

“…B2M/PD-1 single and double genes knock-out T cells were constructed respectively and successively. …”
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Knocking out Fcgr1 of dorsal root ganglion attenuates the activation of NF-κB/NLRP3 pathway in a rat model of rheumatoid arthritis by LIU Fan, SU Si, WANG Tao, YUAN Bo, MA Chao

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Function of molecular chaperones in the secretion of two-partner-secretion to the periplasm in Gram-negative bacteria by ZHANG Yan-juan, WANG Zhe, CAI Kun, Huang Pi-ying, CHU Yin-di, FAN En-guo

Subjects: “…two-partner-secretion|molecular chaperone|periplasm|gene knock-out|gram-negative bacteria…”
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Research of Analysis and Diagnosis of Abnormal Noise at Idle Speed of a Truck Transmission by Liangliang Zhao, Qiang Wu, Fuyun Liu, Hanhong Yu, Rukai Hu

“…Aiming at the problem of transmission gear idling knocking abnormal noise in a batch of domestically produced new trucks， based on the research method of "conjecture-theoretical research-test verification"， the situation that the transmission idling knocking problem is caused by the friction clutch first-stage torsion damper and mismatch of parameters and power transmission system is analyzed and confirmed. …”
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Total flavonoids of Hippophae rhamnoides L. inhibits proliferation of bladder cancer cell line T24 by ZHANG Peibo, LI Yi

“…The same methods were used to detect the effects of knocking down of miR-144 on cell proliferation and apoptosis of T24 cell. …”
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Stable knockout of Mcart-1 down-regulates the proliferation and oxidative phosphorylation of RAW264.7 macrophages by ZHAO Yongjing, QIU Jiaxing, ZHANG Diya, GUO Hongjiang, WANG Yucheng, JU Rui, GUO Lei

“…Results Successfully constructed RAW264.7 cell strain with Mcart-1 stably knocked out, denoted as Mcart-1-/--RAW264.7; A frameshift mutation occurred in Mcart-1 gene in this cell strain; Compared with the wild-type RAW264.7 cell line (RAW264.7), the OCR increased and the extracellular acidification rate(ECAR)of Mcart-1-/--RAW264.7 cells decreased; The total ATP content in Mcart-1 knocked out cells decreased(P＜0.05),mitochondrial ATP production rate decreased (P＜0.01), while glycolytic ATP production rate increased (P＜0.01); The proliferation activity of RAW264.7 cells decreased after Mcart-1 was knocked out(P＜0.001). …”
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FOXM1 promotes proliferation of lung adenocarcinoma cells by regulating HJURP by ZHA Haoran, LI Zhaoxia

“…The expression of HJURP decreased after knocking down FOXM1(P < 0.05). Conclusion HJURP is involved in the regulation of the proliferation of LUAD cells. …”
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Effect of Piezo1 on osteogenic differentiation of mouse bone marrow mesenchymal stem cells C3H10T1/2 based on CRISPR/Cas9 by GAO Xin, YANG Yiling, HUANG Xiangru, DAI Qinggang, JIANG Lingyong

“…CCK-8 assay showed that the cell proliferation ability decreased after knocking out Piezo1 (P<0.05); The results of ALP staining and alizarin red staining showed that the osteogenic ability of cells decreased after knocking out Piezo1(P<0.05). …”
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Construction of Staphylococcus aureus bioluminescence tracing system based on Antares2 by HU Zhen, SHANG Weilong, RAO Xiancai

“…The gene encoding fusion luciferase Antares2 was knocked in the genome of S. aureus strain USA300 by fusing with the enolase (Eno) encoding gene. …”
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LncRNA-ATB inhibits human keloid fibroblast proliferation and collagen synthesis by LYU Xiong, QIU Ling-jiang, LAO Xun-ji, TAN Wei-qiang

“…Conclusions Knock-down of lncRNA-ATB expression may inhibit KFB proliferation and collagen synthesis by down-regulating NF-κB signaling pathway.…”
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CENPF Promotes EMT in Non-small Cell Lung Cancer by Up-regulating ACKR3/CXCR7 by GU Tong, JIANG Yuheng, DING Shu, CHEN Wei, LUO Chao, YU Weiyong, CHEN Xiaofei

“…After the expression was CENPF of knocked out, the cell proliferation, migration, and invasion abilities significantly reduced (P < 0.01). …”
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E3 ubiquitinase RNF138 inhibits the development of ulcerative colitis by DENG Boya, LU Yalan, YU Si, ZHANG Yiyao, HUANG Jingyi, LI Yue, LIU Changzheng

“…Knocking out RNF138 promoted upregulation of p-p65 and its target gene expression(P＜0.05), indicating NF-кB signal pathway activation. …”
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MST1 regulates hepatic gluconeogenesis by promoting PEPCK expression in mice by XIE Xiang-hong, GENG Chao, ZHAO Wei, LI Chun-mei, ZHANG Wei-hong, YANG Jia-hui, LIU Xiao-jun

“…Forkhead transcription factor O1 (FOXO1) was knocked down by interfering adenovirus in primary mouse liver cells to detect the effect of MST1 overexpression on the mRNA levels of PEPCK. …”
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Indoleamine 2,3-dioxygenase 1 inhibitor Epacadostat combined with IFN-γ induces apoptosis of triple-negative breast cancer cell lines by HU Qing-qing, LU Yan

“…The expression of IDO1 was detected by Western blot and the yield of Kyn in 4T1or MDA-MB-231 cell lysate was detected by ELISA. The IDO1 was knocked out by using CRISPR-Cas9 system and knockout efficiency was examined by Western blot. …”
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RBX1 regulates uveal melanoma immune-related genes via STAT1 by ZHOU Xiaowen, LI Qian, ZHANG Zhe, SHEN Jianfeng, FAN Xianqun

“…RBX1 was transiently knocked down in the UVM cell lines, i.e., 92.1, OMM2.3 and MEL290 by using small interfering RNAs (siRNAs) targeting RBX1. …”
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HUVECs with KNDC1 knockout mediated by CRISPR/Cas9 have anti-aging ability by SUN Jing-yu, YAO He, HU Gang, WEI Jie, GUO Jun, ZHANG Xin, LIN Ya-jun

“…Objective To knock out the KNDC1 of human umbilical vein endothelial cells (HUVECs) by CRISPR/Cas9 gene editing technology and observe its anti-aging ability. …”
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1α,25-Dihydroxyvitamin D3 inhibits β-catenin transcriptional activity in human colon cancer cell line SW480 by XIN Yu, HE Long-mei, YANG Hong, LYU Hong, TAN Bei, WANG Hong-ying, QIAN Jia-ming

“…SW480 cells were subjected to transfecting siRNA to knock down FOXM1, followed by 1,25(OH)2D3 treatment and measurement of the transcriptional activity of β-catenin by Dual-Luciferase? …”
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Expression and mechanism of gp73 in liver tissue of mouse liver fibrosis by MA Ling-yu, ZHEN Yi-ning, LUO Yun-ping, DUAN Zhao-jun

“…Next, IGF2BP3 over-expression and knock down plasmids were transfected, and the changes of GOLM1, which encodes gp73, were detected by RT-PCR. …”
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