Rapid and Scalable Production of Functional SARS-CoV-2 Virus-like Particles (VLPs) by a Stable HEK293 Cell Pool by Puarattana-aroonkorn, Sitthiphol, Tharakaraman, Kannan, Suriyawipada, Disapan, Ruchirawat, Mathuros, Fuangthong, Mayuree, Sasisekharan, Ram, Artpradit, Charlermchai

“…We employed the membrane, envelope, and highly immunogenic spike proteins of SARS-CoV-2 to produce virus-like particles (VLPs) using the HEK293-F cell line as a host system with an economical transfection reagent. …”
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Cytoplasmic long noncoding RNAs are frequently bound to and degraded at ribosomes in human cells by Carlevaro-Fita, Joana, Rahim, Anisa, Guigó, Roderic, Johnson, Rory, Vardy, Leah Karen Anne

“…Here we make a global, absolute estimate of the cytoplasmic and ribosome-associated population of stringently filtered lncRNAs in a human cell line using polysome profiling coupled to spike-in normalized microarray analysis. Fifty-four percent of expressed lncRNAs are detected in the cytoplasm. …”
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Back to the Basics of SARS-CoV-2 Biochemistry: Microvascular Occlusive Glycan Bindings Govern Its Morbidities and Inform Therapeutic Responses by Scheim, David E., Parry, Peter I., Rabbolini, David J., Aldous, Colleen, Yagisawa, Morimasa, Clancy, Robert, Borody, Thomas J., Hoy, Wendy E.

“…Consistent with the biochemistry of coronaviruses as well established over decades, SARS-CoV-2 makes its initial attachment to host cells through the binding of its spike protein (SP) to sialylated glycans (containing the monosaccharide sialic acid) on the cell surface. …”
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NMDA receptor activation enhances inhibitory GABAergic transmission onto hippocampal pyramidal neurons via presynaptic and postsynaptic mechanisms by Xue, Jiu Gang, Masuoka, Takayoshi, Gong, Xian Di, Chen, Ken-Shiung, Yanagawa, Yuchio, Law, Alex Sai Kit, Konishi, Shiro

“…Thus NMDARs at glutamatergic synapses on both CA1 pyramidal neurons and interneurons appear to exert feedback and feedforward inhibition for determining the spike timing of the hippocampal microcircuit.…”
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Liquid state machine with dendritically enhanced readout for low-power, neuromorphic VLSI implementations by Roy, Subhrajit, Banerjee, Amitava, Basu, Arindam

“…We show that compared to a single perceptron using analog weights, this architecture for the readout can attain, even by using the same number of binary valued synapses, up to 3.3 times less error for a two-class spike train classification problem and 2.4 times less error for an input rate approximation task. …”
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Characterising the T cell responses to SARS-CoV-2 including variants of concern induced by natural infection and vaccines by Adele, S

“…Chapter 4 expands on T cell responses to VOCs, showing that vaccinated individuals maintain significant T cell reactivity across VOCs despite spike protein mutations that diminish antibody neutralization efficacy. …”

Seroepidemiology of COVID-19 in pregnant women and their infants in Uganda and Malawi across multiple waves 2020-2022 by Hookham, L, Cantrell, L, Cose, S, Freyne, B, Gadama, L, Imede, E, Kawaza, K, Lissauer, S, Musoke, P, Nankabirwa, V, Sekikubo, M, Sommerfelt, H, Voysey, M, PeriCOVID Consortium, Le Doare, K

“…The placental transfer GMR was 1.7, indicating active placental transfer of anti-spike IgG. There was no association between SARS-CoV-2 antibody positivity and adverse pregnancy or infancy outcomes. …”

A hand-targeted auxiliary personal protective equipment for intervention of fomite transmission of viruses by Tan, Justin Kok Soon, Song, Shang Wei, Zeng, Jialiu, Lo, Chih Hung

“…Compared to conventional glove materials, FlexiPalm demonstrated enhanced mechanical durability and breathability, comparable hydrophobicity, and displayed a minimal adsorption of SARS-CoV-2 spike protein and virus-like particles (VLP). Importantly, FlexiPalm was found to bind significantly less viral protein and VLP than artificial human skin, confirming its ability to reduce viral contamination. …”
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High-performance one-dimensional halide perovskite crossbar memristors and synapses for neuromorphic computing by Vishwanath, Sujaya Kumar, Febriansyah, Benny, Ng, Si En, Das, Tisita, Acharya, Jyotibdha, John, Rohit Abraham, Sharma, Divyam, Dananjaya, Putu Andhita, Jagadeeswararao, Metikoti, Tiwari, Naveen, Kulkarni, Mohit Ramesh Chandra, Lew, Wen Siang, Chakraborty, Sudip, Basu, Arindam, Mathews, Nripan

“…This consequently facilitates the demonstration of accurate handwritten digit recognition from the MNIST database based on spike-timing-dependent plasticity of halide perovskite memristive synapses.…”
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Generation of self-replicating airway organoids from the cave nectar bat Eonycteris spelaea as a model system for studying host-pathogen interactions in the bat airway epithelium by Chan, Louisa L. Y., Gamage, Akshamal M., Tan, Chee Wah, Tan, Kai Sen, Liu, Jing, Tay, Douglas Jie Wen, Foo, Randy Jee Hiang, Rénia, Laurent, Wang, De Yun, Wang, Lin-Fa

“…However, these ALI-AECs did not support SARS-CoV-2 infection, despite E. spelaea ACE2 receptor being capable of mediating SARS-CoV-2 spike pseudovirus entry. This work provides critical model systems for assessing bat species-specific virus susceptibility and the reservoir likelihood for emerging infectious agents.…”
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Interaction-mediating sequences within class I viral fusion glycoproteins : their roles in viral infection and in applications by Zhang, Simin

“…In Paper I, peptide array scanning identified 34 spike (S) protein-derived peptides that bound to the S protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV). …”
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EEG-based attention recognition using machine learning by Sun, Haoqi

“…To address this issue, a novel bio-inspired algorithm, joint weight-delay spike-timing dependent plasticity (joint STDP), is proposed for learning sparse hidden weights and EEG spectral features. …”
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Employment of a high throughput functional assay to define the critical factors that influence vaccine induced cross-variant neutralizing antibodies for SARS-CoV-2 by Gu, Yue, Shunmuganathan, Bhuvaneshwari, Qian, Xinlei, Gupta, Rashi, Tan, Rebecca S. W., Kozma, Mary, Purushotorman, Kiren, Murali, Tanusya M., Tan, Nikki Y. J., Preiser, Peter Rainer, Lescar, Julien, Nasir, Haziq, Somani, Jyoti, Tambyah, Paul A., Smith, Kenneth G. C., Renia, Laurent, Ng, Lisa F. P., Lye, David C., Young, Barnaby E., MacAry, Paul A.

“…We also present an accurate quantitation of serological responses for SARS-CoV-2 that exploits a unique set of in-house, recombinant human monoclonal antibodies targeting the viral Spike and nucleocapsid proteins and demonstrate a reduction in neutralizing antibody titres across all groups 6 months post-vaccination. …”
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Immunogenicity and safety of beta variant COVID-19 vaccine AZD2816 and AZD1222 (ChAdOx1 nCoV-19) as primary-series vaccination for previously unvaccinated adults in Brazil, South A... by Costa Clemens, SA, Jepson, B, Bhorat, QE, Ahmad, A, Akhund, T, Aley, PK, Bansal, H, Bibi, S, Kelly, EJ, Khan, M, Lambe, T, Lombaard, JJ, Matthews, S, Pipolo Milan, E, Olsson, U, Ramasamy, MN, Moura de Oliveira Paiva, MS, Seegobin, S, Shoemaker, K, Szylak, A, Villafana, T, Pollard, AJ, Green, JA

“…<p><b>Background</b></p> AZD2816 is a variant-adapted COVID-19 vaccine that expresses the full-length SARS-CoV-2 beta variant spike protein but is otherwise similar to AZD1222 (ChAdOx1 nCoV-19). …”

Social tags as news event detectors by Chua, Alton Yeow Kuan, Razikin, Khasfariyati, Goh, Dion Hoe-Lian

“…First, the performance of using spikes and bursts to detect news events was found to be comparable. …”
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Tunable anti-ambipolar vertical bilayer organic electrochemical transistor enable neuromorphic retinal pathway by Laswick, Zachary, Wu, Xihu, Surendran, Abhijith, Zhou, Zhongliang, Ji, Xudong, Matrone, Giovanni Maria, Leong, Wei Lin, Rivnay, Jonathan

“…The vertical OECT design reduces device footprint, while the bilayer material tuning controls the anti-ambipolarity characteristics, allowing control of the device's on and off threshold voltages, and peak position, while reducing size thereby enabling tunable threshold spiking neurons and logic gates. Combining these components, a mimic of the retinal pathway reproducing the wavelength and light intensity encoding of horizontal cells to spiking retinal ganglion cells is demonstrated. …”
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Investigating the potential of axonopus compressus to phytoremediate molybdenum by Eng, Zhi Xian

“…Mo accumulation in dry tissue of AC peaked in Week 1 and gradually decreased with time. Control AC and pots spiked with more than 20 ppm exhibited stunted growth two weeks after treatment. …”
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An on-nanoparticle rolling-circle amplification platform for ultrasensitive protein detection in biological fluids by Yan, Juan, Song, Shiping, Li, Bing, Zhang, Qingzhi, Huang, Qing, Zhang, Hua, Fan, Chunhai

“…The nanoRCA assay is applicable to the detection of two tumor markers in biological fluids: prostate-specific antigen in spiked sera and interleukin-8 in saliva.…”
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Microfluidic platform for negative enrichment of circulating tumor cells by Sajay, Bhuvanendran Nair Gourikutty, Chang, Chia-Pin, Ahmad, Hamizah, Khuntontong, Puttachat, Wong, Chee Chung, Wang, Zhiping, Puiu, Poenar Daniel, Soo, Ross, Rahman, Abdur Rub Abdur

“…This negative enrichment method employs upstream immunomagnetic depletion to deplete CD45-positive WBCs followed by a microfabricated filter membrane to perform chemical-free RBC depletion and target cells isolation. Experiments of spiking two cell lines, MCF-7 and NCI-H1975, in the whole blood show an average of >90 % cell recovery over a range of spiked cell numbers. …”
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Exposure to Pb-halide perovskite nanoparticles can deliver bioavailable Pb but does not alter endogenous gut microbiota in zebrafish by Patsiou, Danae, del Rio-Cubilledo, Cristina, Catarino, Ana Isabel, Summers, Stephen, Afiq Mohd Fahmi, Boyle, David, Fernandes, Teresa F., Henry, Theodore B.

“…Induction of mt2 and reduction of ala-d expression levels in liver tissues showed Pb bioavailability after 2-d and 4-d dietary exposure to perovskite-spiked feeds. Changes in gut microbiota of adult zebrafish were detected after 14-d exposure to Pb-spiked food, but no changes were detected from perovskite-NP spiked food. …”
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