DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts by Kuś, K, Carrique, L, Kecman, T, Fournier, M, Hassanein, SS, Aydin, E, Kilchert, C, Grimes, JM, Vasiljeva, L

“…Spt5 is also a key factor that attenuates the expression of non-coding transcripts, coordinates pre-mRNA splicing and 3’-end processing. Our findings indicate that engagement with the transcribing Pol II is an essential regulatory step modulating the activity of RNA enzymes such as Xrn2, thus advancing our understanding of how RNA maturation is controlled during transcription.…”

De novo genome sequence assembly of the RNAi-tractable endosymbiosis model system Paramecium bursaria 186b reveals factors shaping intron repertoire by Leonard, G, Jenkins, BH, Savory, FR, Kilias, ES, Maguire, F, Varma, V, Milner, DS, Richards, TA

“…To explore intron evolutionary dynamics further we show that alternative splicing leading to intron retention occurs at a higher frequency among the smaller number of longer introns, identifying a source of selection against longer introns. …”

Computational analysis and prediction of specific genomic regions forming R-loop structure and chromosomal variations associated with cancer by Wongsurawat, Thidathip

“…Integrative genomics analyses suggested that RLFSs could play a role in gene regulation, AID/APOBEC-mediated editing/mutagenesis, alternative splicing, and epigenetic modifications, and also associate with mutations in cancer, neurodegenerative diseases and mental disorders. …”
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Potential ago2/miR-3068-5p cascades in the nucleus accumbens contribute to methamphetamine-induced locomotor sensitization of mice by Liu, Dan, Liang, Min, Zhu, Li, Zhou, Ting-Ting, Wang, Yu, Wang, Rui, Wu, Fei-Fei, Goh, Eyleen Lay Keow, Chen, Teng

“…Argonaute2 (Ago2), a specific splicing protein involved in the generation of miRNA, was found to be dysregulated in the nucleus accumbens (NAc) of methamphetamine (METH)-sensitized mice in our previous study. …”
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Investigating the human teashirt genes TSHZ2 and TSHZ3 in pancreatic and endocrine lineage commitment using genome modified human embryonic stem cells (hESC) by Eng, Shermaine Zi Hui

“…To better understand the role of these genes in human pancreatic development, the expression of TSHZ2 Variant 1 and TSHZ3 was characterized, revealing a novel spliced variant of TSHZ2 Variant 1. The CRISPR RNA-guided FokI-dCas9 nuclease (RFN) genome editing system was employed to create loss-of-function hESC lines. …”
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Inhibitory Potential of the Truncated Isoforms on Glutamate Transporter Oligomerization Identified by Computational Analysis of Gene-Centric Isoform Maps by Karagöl, Alper, Karagöl, Taner, Li, Mengke, Zhang, Shuguang

“…Conclusion Our computational studies on glutamate transporters offer insights into the roles of alternative splicing on protein interactions and identifies potential drug targets for physiological or pathological processes.…”
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Diagnosis of age-related macular degeneration (AMD) using optical fiber sensor by Muhamad Nur Azhar Noordin

“…The FPI consists of a standard single-mode fiber spliced with 1mm of hollow-core fiber and 0.8mm of no-core fiber. …”
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Pan-cancer analysis of whole genomes by ICGC/TCGA Pan-Cancer Analysis of Whole Genomes Consortium

“…A collection of papers from the PCAWG Consortium describes non-coding mutations that drive cancer beyond those in the TERT promoter; identifies new signatures of mutational processes that cause base substitutions, small insertions and deletions and structural variation; analyses timings and patterns of tumour evolution; describes the diverse transcriptional consequences of somatic mutation on splicing, expression levels, fusion genes and promoter activity; and evaluates a range of more-specialized features of cancer genomes.…”

Runx family genes in a cartilaginous Fish, the elephant Shark (Callorhinchus milii) by Nah, Giselle Sek Suan, Lim, Zhi Wei, Tay, Boon-Hui, Osato, Motomi, Venkatesh, Byrappa

“…Through the use of alternative promoters and/or alternative splicing, each of the elephant shark Runx genes expresses multiple isoforms similar to their orthologs in human and other bony vertebrates. …”
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Wolbachia-mosquito interactions and engineered female-specific lethality for Aedes-arbovirus control by Molloy, JC

“…In Chapter 4, I engineer female-specific, late-acting lethality in <em>Ae. aegypti</em> using specific effectors crossed with candidate pupal-stage promoters and a <em>doublesex</em> cassette causing sexspecific splicing. In Chapter 5, I transform and characterise <em>Ae. aegypti</em> lines producing sex-specific <em>Drosophila melanogaster</em> alcohol dehydrogenase (<em>Adh</em>) to evaluate potential for early-stage inducible genetic sexing. …”

基于高掺锗石英光纤的中红外超连续谱产生 = Mid-infrared supercontinuum generation with highly germanium-doped silica fiber by 朱磊 Zhu Lei, 王鹿鹿 Wang Lulu, 董新永 Dong Xinyong, 沈平 Shen Ping, 苏海斌 Su Haibin

“…The free space coupling efficiency from the pump laser to the germanium-doped fiber is enhanced by fusion-splicing a 100~200 μm long multimode fiber. Length of germanium-doped fiber is optimized. …”
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Securing untrusted memories in embedded systems by Vig, Saru

“…This thesis aims to address this challenge by proposing compile-time and runtime strategies for securing untrusted memories from active memory attacks (e.g. replay, spoo ng and splicing attacks) that can compromise the con dentiality and integrity of the memory data. …”
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Conformational design characterization of a truncated diamine oxidase by Razali, Nur Nadia

“…The domain 4 that contained the active site was selected for the splicing process in order to retain the enzyme’s active site. …”
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