Alterations in Ascorbic Acid Levels by Transformation of the L-galactono-1,4-lactone Dehydrogenase Gene in Solanum tuberosum by Dong Yumei, Li Yansu, Jiao Zigao, Li Chaohan, Yu Xianchang

“…The recombinants were introduced into potato via Agrobacterium-mediated transformation, and plants were confirmed as transgenic using PCR and quantitative real-time PCR. …”
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Prediction and optimization of indirect shoot regeneration of Passiflora caerulea using machine learning and optimization algorithms by Marziyeh Jafari, Mohammad Hosein Daneshvar

“…Abstract Background Optimization of indirect shoot regeneration protocols is one of the key prerequisites for the development of Agrobacterium-mediated genetic transformation and/or genome editing in Passiflora caerulea. …”
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Targeted editing of OsSWEET11 promoter for imparting bacterial leaf blight resistance in rice by D. Bhagya Sree1 , A. Shanthinie1 , P. Vignesh2 , S. Varanavasiappan1 , K. K. Kumar1 , L. Arul1 , E. Kokiladevi1 , S. Manonmani3 , N. Saranya4 and D. Sudhakar1*

“…Genome editing of ASD16, through Agrobacterium-mediated transformation resulted in seventeen transgenic events. …”
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CRISPR/Cas9-Mediated Mutagenesis of the Granule-Bound Starch Synthase Gene in the Potato Variety Yukon Gold to Obtain Amylose-Free Starch in Tubers by Stephany Toinga-Villafuerte, Maria Isabel Vales, Joseph M. Awika, Keerti S. Rathore

“…Potato varieties with higher amylopectin have many food processing and industrial applications. Using <i>Agrobacterium</i>-mediated transformation, we delivered Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9 (CRISPR/Cas9) reagents to potato (variety Yukon Gold) cells to disrupt the granule-bound starch synthase (<i>gbssI</i>) gene with the aim of eliminating the amylose component of starch. …”
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Morphological and molecular characterization of bacterial endophytes from Centella asiatica leaves by Siphiwe G. Mahlangu, Siew L. Tai

“…Based on molecular traits, the isolates were identified as Pseudomonas sp. strain SGM1, Pseudomonas sp. strain SGM2, Pseudomonas sp. strain SGM3, Pseudomonas sp. strain SGM4, Pseudomonas sp. strain SGM5, Pseudomonas sp. strain SGM6, Pseudomonas sp. strain SGM7, Novosphingobium sp. strain SGM8, Pseudomonas sp. strain SGM9, Pseudomonas sp. strain SGM10, Chryseobacterium sp. strain SGM11, Enterobacter sp. strain SGM12, Enterobacter sp. strain SGM13, Pseudomonas sp. strain SGM14, Enterobacter sp. strain SGM15, Enterobacter sp. strain SGM16, Agrobacterium sp. strain SGM17, Pantoea sp. strain SGM18, Paraburkholderia sp. strain SGM19, and Pseudomonas sp. strain SGM20. …”
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Establishment of virus-induced gene silencing (VIGS) system in Luffa acutangula using Phytoene desaturase (PDS) and tendril synthesis related gene (TEN) by Xiaoyu Qi, Qiaoping Mo, Jing Li, Zhibo Zi, Mengyun Xu, Suju Yue, Hongbo Zhao, Haisheng Zhu, Guoping Wang

“…Plants infected with Agrobacterium harboring pV190-PDS successfully induced effective silencing in Luffa, and an effective gene silencing phenotype with obvious photobleaching was observed. …”
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Deciphering the underlying immune network of the potato defense response inhibition by Phytophthora infestans nuclear effector Pi07586 through transcriptome analysis by Yumeng Xiong, Yumeng Xiong, Di Zhao, Shengnan Chen, Lan Yuan, Die Zhang, Hongyang Wang

“…Quantitative real-time PCR (qRT-PCR) analysis showed Pi07586 was significant up-regulation during the early stages of infection. Agrobacterium-induced transient expression revealed that Pi07586 localized in the nucleus of leaf cells. …”
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Transcription factors Mat2 and Znf2 operate cellular circuits orchestrating opposite- and same-sex mating in Cryptococcus neoformans. by Xiaorong Lin, Jennifer C Jackson, Marianna Feretzaki, Chaoyang Xue, Joseph Heitman

“…Here, we applied insertional mutagenesis via Agrobacterium-mediated transformation and transcription analysis using whole genome microarrays to identify factors involved in C. neoformans differentiation. …”
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Phylogenetic and structural analysis of annexins in pea (<i>Pisum sativum</i> L.) and their role in legume-rhizobial symbiosis development by O. A. Pavlova, I. V. Leppyanen, D. V. Kustova, A. D. Bovin, E. A. Dolgikh

“…To follow the PsAnn4 protein localization in plant cells, it was fused to the fluorophores such as red fluorescent protein (RFP) and yellow fluorescent protein (YFP) and expressed under the transcriptional regulation of the 35S promoter in Nicotiana benthamiana leaves by infiltration with Agrobacterium tumefaciens. The localization of PsAnn4 in the cell wall or plasma membrane of plant cells may indicate its participation in membrane modification or ion transport. …”
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Optimization of the Cry2Aa gene and development of insect-resistant and herbicide-tolerant photoperiod-sensitive genic male sterile rice by Lushui Weng, Lihua Deng, Fengxiang Lai, Guoying Xiao

“…The Cry2Aa# and Bar genes were transformed into 4008S, a photoperiod-sensitive genic male sterile (PGMS) line in rice, using the Agrobacterium transfer method. A total of 65 regenerated plantlets confirmed by PCR were produced, in which eight transgenic lines had single-copy insertions as confirmed by Southern blot analysis. …”
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Critical role for uricase and xanthine dehydrogenase in soybean nitrogen fixation and nodule development by Cuong X. Nguyen, Alice Dohnalkova, C. Nathan Hancock, Kendall R. Kirk, Gary Stacey, Minviluz G. Stacey

“…Using a forward genetic approach, as well as CRISPR/Cas9 gene editing via Agrobacterium rhizogenes‐mediated hairy root transformation, we identified and characterized the role of GmUOX (Uricase) and GmXDH (Xanthine Dehydrogenase) in nitrogen fixation and nodule development in soybean. …”
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Response and Function of <i>Solanum lycopersicum</i> L. <i>SlSGR2</i> Gene under Cadmium Stress by Jianyu Ma, Xuanming Dong, Lijie Yu, Yuhong Zhang

“…The pCAMBIA2300-<i>SlSGR2</i> overexpression constructs were introduced into wild-type <i>Arabidopsis</i> plants via <i>Agrobacterium</i>-mediated transformation. Homozygous mutant lines of <i>sgr2</i> were identified using the three-primer method. …”
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CRISPR-mediated genome editing in poplar issued by efficient transformation by Ali Movahedi, Ali Movahedi, Hui Wei, Saeid Kadkhodaei, Weibo Sun, Qiang Zhuge, Liming Yang, Chen Xu

“…Our findings confirmed that Agrobacterium inoculator OD600 = 2.5, increased DDT numbers during cell division to 4:1 pDDT/pgRNA, and optimized homologous arms 700 bp caused efficient HDR and increased MKK2 expression.ConclusionEfficient transformations resulted from optimized variables, directly affecting the HDR efficiency through woody plants such as poplar.…”
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Human Indoleamine 2,3-dioxygenase 1 (IDO1) Expressed in Plant Cells Induces Kynurenine Production by Michele Bellucci, Andrea Pompa, Carine De Marcos Lousa, Eleonora Panfili, Elena Orecchini, Elisa Maricchiolo, Daniele Fraternale, Ciriana Orabona, Francesca De Marchis, Maria Teresa Pallotta

“…Here, we attempted to produce kynurenine, a health-promoting metabolite, in plants of <i>Nicotiana tabacum</i> (tobacco) transformed by <i>Agrobacterium tumefaciens</i> with the gene, coding for human indoleamine 2,3-dioxygenase 1 (IDO1), an enzyme responsible for the kynurenine production because of tryptophan degradation. …”
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Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing by Sally Hassan, Mohamed Abdel-Salam, ayman hanfy, Haytham Abd-Elhalim, Nouh Ahmed, Emad Metry

“…In this study, the CRISPR technology ″Clustered Regularly Interspaced Short Palindromic Repeats″ has been used in potato cultivar Desiree via Agrobacterium mediated transformation to edit starch phosphorylase gene that is responsible for starch degradation in cold temperature storage, after regeneration steps. …”
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Functional Analysis of the <i>Lupinus luteus</i> Cyclophilin Gene Promoter Region in <i>Lotus japonicus</i> by Katarzyna Nuc, Przemysław Olejnik, Mirella Samardakiewicz, Przemysław Nuc

“…We used <i>Lotus japonicus</i> as a model plant. After <i>Agrobacterium rhizogenes</i> transformation of <i>L. japonicus</i>, only the longest promoter region (−1055 bp to ATG) supported the β-glucuronidase expression in root nodule parenchyma. …”
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Overexpression of <i>RrGGP2</i> and <i>RrDHAR</i> Increases Ascorbic Acid Content in Tomato by Zeyang Liu, Tianzhi Rao, Richard A. Ludlow, Yali Yan, Min Lu, Huaming An

“…The overexpression vectors were made using 35S promoters and mediated by <i>Agrobacterium tumefaciens</i> to obtain the overexpression lines. …”
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Isolation and antisense suppression of <it>flavonoid 3', 5'-hydroxylase </it>modifies flower pigments and colour in cyclamen by Patel Deepa, Marshall Gayle B, Davies Kevin M, Lewis David H, Boase Murray R, Schwinn Kathy E, Deroles Simon C

“…Two cultivars of minicyclamen were transformed via <it>Agrobacterium tumefaciens </it>with an antisense <it>CpF3'5'H </it>construct. …”
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Synthesis, Characterization and Application of Novel Cationic Surfactants as Antibacterial Agents by Shaban R. M. Sayed, Abdelrahman O. Ezzat, Mohamed Taha Yassin, Ashraf M. M. Abdelbacki

“…Meanwhile, it recorded inhibition zone values of 17.5, 25.2 and 23.8 mm for <i>Escherichia coli</i>, <i>Agrobacterium tumefaciens</i> and <i>Erwinia carotovora</i>, respectively, at 200 μg/disk. …”
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Enhanced tolerance to low-K+ stress in tobacco plants, that ectopically express the CBL-interacting protein kinase CIPK23 gene by Gang XUE, Li-Ming LU, Tie-Zhao YANG, Xiao-Hui LI, Xue-Xia XING, Shi-Xiao XU

“…Molecular analyses revealed that the AtCIPK23 gene was successfully transferred into a tobacco cultivar K326 via Agrobacterium tumefaciens-mediated transformation. Overexpression of AtCIPK23 in tobacco resulted in increased low-K+ tolerance, which was demonstrated by higher dry biomass, longer primary root length, higher K+ content and better growth status of transgenic tobacco plants compared to controls when both were treated in low-K+ MS medium and low-K+ hydroponics. …”
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