Efficacy of a plant-produced infectious bronchitis virus-like particle vaccine in specific pathogen-free chickens by Kamogelo M. Sepotokele, Martha M. O'Kennedy, Michaela C. Hayes, Daniel B.R. Wandrag, Peter Smith, Celia Abolnik

“…Its high mutation ability has resulted in numerous variants against which the commercially available live or recombinant vaccines singly offer limited protection. Agrobacterium-mediated transient expression in Nicotiana benthamiana (tobacco) plants was used here to produce a virus-like particle (VLP) vaccine expressing a modified full-length IBV spike (S) protein of a QX-like IB variant. …”
Get full text

A Sinorhizobium meliloti-specific N-acyl homoserine lactone quorum-sensing signal increases nodule numbers in Medicago truncatula independent of autoregulation by Debora Fabiola Veliz Vallejos, Giel Eduard van Noorden, Mengqi eYuan, Ulrike eMathesius

“…We treated roots of the model legume, Medicago truncatula, with a range of AHLs either from its specific symbiont, Sinorhizobium meliloti, or from the potential pathogens, Pseudomonas aeruginosa and Agrobacterium vitis. We found increased numbers of nodules formed on root systems treated with the S. meliloti-specific AHL, 3-oxo-C14-homoserine lactone, at a concentration of 1 μM, while the other AHLs did not result in significant changes to nodule numbers. …”
Get full text

Desarrollo de un sistema de selección positiva para plantas transgénicas de papa (Solanum tuberosum SSP andígena var diacol capiro) by Mary Luz Yaya Lancheros, Alejandro Chaparro Giraldo

“…Con el objetivo de desarrollar un sistema de selección pmi/manosa para la variedad Diacol Capiro, se establecieron las condiciones necesarias para la transformación de entrenudos con la cepa LBA4404 de Agrobacterium tumefaciens que contiene el vector binario pNOV022, obteniéndose tasas regenerativas del 80-100% y plantas entre la séptima y novena semana. …”
Get full text

Highly Efficient Expression of Tannase in Aspergillus niger and Its Enzymatic Properties by Danxin TONG, Jie LI, Hongbin HE, Hui ZHANG

“…GlaA multi-copy strong promoter PglaA6R and signal peptide SglaA were integrated to construct tannase gene AnTan Aspergillus niger recombinant expression vector pSZHG6R-AnTan with the endogenous highly expressed glaA gene site as the integration target site, and the Aspergillus niger pure recombinant strain A1 of tannase was obtained by agrobacterium-mediated transformation of Aspergillus niger. …”
Get full text

Overexpression of an Inositol Phosphorylceramide Glucuronosyltransferase Gene <i>IbIPUT1</i> Inhibits Na⁺ Uptake in Sweet Potato Roots by Chong Liu, Mingku Zhu, Jian Sun

“…Herein, the <i>IbIPUT1</i> gene, which encodes an ortholog of <i>Arabidopsis AtIPUT1</i>, from sweet potato was cloned. <i>Agrobacterium rhizogenes</i>-mediated in vivo transgenic technology, non-invasive micro-measuring technology (NMT) and Na⁺ fluorescence imaging technology were then combined to quickly study the potential function of <i>IbIPUT1</i> in salt tolerance. …”
Get full text

How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters by Yane Caroline dos Anjos Bezerra, João Paulo Rodrigues Marques, Liliane Cristina Liborio Stipp, Lísia Borges Attílio, Juliana Freitas-Astúa, Francisco de Assis Alves Mourão Filho

“…Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (ß-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. …”
Get full text

Expression of the grape VqSTS21 gene in Arabidopsis confers resistance to osmotic stress and biotrophic pathogens but not Botrytis cinerea by Li Huang, Songlin Zhang, Stacy Dawn Singer, Xiangjing Yin, Jinhua Yang, Yuejin Wang, Xiping Wang

“…To provide a deeper understanding of the function of this gene, we cloned the full-length coding sequence of VqSTS21 and overexpressed it in Arabidopsis thaliana via Agrobacterium-mediated transformation. The resulting VqSTS21 Arabidopsis lines produced trans-piceid rather than resveratrol as their main stilbenoid product and exhibited improved disease resistance to PM and Pseudomonas syringae pv. tomato DC3000, but displayed increased susceptibility to Botrytis cinerea. …”
Get full text

Virus-Induced Gene Silencing (VIGS) in Chinese Jujube by Yao Zhang, Nazi Niu, Shijia Li, Yin Liu, Chaoling Xue, Huibin Wang, Mengjun Liu, Jin Zhao

“…After the cotyledon was fully unfolded, <i>Agrobacterium</i> mixture containing pTRV1 and pTRV2-ZjCLA with OD₆₀₀ = 1.5 was injected into cotyledon. …”
Get full text

The sweet potato transcription factor IbbHLH33 enhances chilling tolerance in transgenic tobacco by Tao Yu, Huanan Zhou, Zhenlei Liu, Hong Zhai, Qingchang Liu

“…Transgenic tobacco plants overexpressing IbbHLH33 were obtained by Agrobacterium-mediated transformation, which enhanced the chilling resistance of tobacco. …”
Get full text

Improved Transformation and Regeneration of <i>Indica</i> Rice: Disruption of <i>SUB1A</i> as a Test Case via CRISPR-Cas9 by Yuya Liang, Sudip Biswas, Backki Kim, Julia Bailey-Serres, Endang M. Septiningsih

“…Here, methods for <i>Agrobacterium</i>-mediated and biolistic transformation and regeneration of <i>indica</i> rice were optimized using CRISPR-Cas9 gene-editing of the submergence tolerance regulator <i>SUBMERGENCE 1A-1</i> gene of the cultivar Ciherang-Sub1. …”
Get full text

Cytokinin biosynthesis in cyanobacteria: Insights for crop improvement by Shashi Uniyal, Munni Bhandari, Preeti Singh, Rahul Kunwar Singh, Shree Prakash Tiwari

“…Previously, it was assumed that plants obtain cytokinin from the soil produced by microbes as these hormones were first discovered in soil-inhabiting bacteria i.e., Agrobacterium tumefaciens. Later, the cytokinin biosynthesis gene, i.e., ipt gene, has been reported in plants too. …”
Get full text

Silver Nanoparticles Synthesized with Extracts of Leaves of Raphanus sativus L, Beta vulgaris L, and Ocimum basilicum and Its Application in Seed Disinfection by Cristina Garcidueñas-Piña, Carolina Tirado-Fuentes, Julio Ruiz-Pérez, Roberto Carlos Valerio-García, José Francisco Morales-Domínguez

“…All AgNPs showed antimicrobial activity against Escherichia coli and Klebsiella oxytoca which are bacteria of clinical interest, and against Agrobacterium tumefaciens, a bacterium used in the genetic transformation of plants.…”
Get full text

Comparative genomics of bacteria from amphibian skin associated with inhibition of an amphibian fungal pathogen, Batrachochytrium dendrobatidis by Noah Wax, Jenifer B. Walke, David C. Haak, Lisa K. Belden

“…We identified differences in Agrobacterium and Sphingomonas isolates in the chitinase genes that showed some association with anti-Bd activity, as well as variation in the violacein genes in the Janthinobacterium isolates. …”
Get full text

IMPROVING ROOTING AND GROWTH OF CONOCARPUS ERECTUS STEM CUTTINGS USING INDOLE-3-BUTYRIC ACID (IBA) AND SOME BIOSTIMULANTS by Sayed Abdel-Rahman, Essam Abdul-Hafeez, Asmaa Saleh

“…The current study was conducted to evaluate the effects of indole-3-butyric acid (IBA) at 100 ppm with or without biostimulants (Agrobacterium rhizogenes, seaweed extract and coconut water) as either a spray or drench for 3 minutes or 1 hour on rooting and growth parameters of three cutting types (tip, middle and basal) of Conocarpus erectus L. …”
Get full text

Injection-based hairy root induction and plant regeneration techniques in Brassicaceae by Veronika Jedličková, Marie Štefková, Terezie Mandáková, Juan Francisco Sánchez López, Marek Sedláček, Martin A. Lysak, Hélène S. Robert

“…Results We initiated hairy root formation in the rare crucifer species Asperuginoides axillaris via an injection-based protocol using the Agrobacterium strain C58C1 harboring a hairy root-inducing (Ri) plasmid and successfully regenerated plants from established hairy root lines. …”
Get full text

Development of a New Genetic Transformation System for White and Green Ash Using Embryogenic Cultures by Andrew Ryan Tull, Heather Gladfelter, Flavia Pampolini, Lynne Rieske, Charles Dana Nelson, Scott Merkle

“…An important requirement for applying RNAi technology is a reliable transformation/regeneration system for the host tree species. We developed an Agrobacterium-mediated gene transfer system for white ash (<i>F. americana</i>) and green ash (<i>F. pennsylvanica</i>) using the embryogenic cultures of these species as target material. …”
Get full text

Transgenic Rice Plants Expressing Artificial miRNA Targeting the Rice Stripe Virus <i>MP</i> Gene Are Highly Resistant to the Virus by Liya Zhou, Quan Yuan, Xuhong Ai, Jianping Chen, Yuwen Lu, Fei Yan

“…In this study, we constructed an amiRNA targeting the RSV MP gene (amiR MP) based on the backbone sequence of the osa-MIR528 precursor, and obtained marker-free transgenic rice plants constitutively expressing amiR MP by <i>Agrobacterium tumefacien</i>s-mediated transformation. …”
Get full text

Identification and Characterization of a Novel Protein Disulfide Isomerase Gene (<i>MgPDI2</i>) from <i>Meloidogyne graminicola</i> by Zhongling Tian, Zehua Wang, Maria Munawar, Jingwu Zheng

“…In addition, an <i>Agrobacterium</i>-mediated transient expression assay found that MgPDI2 caused noticeable cell death in <i>Nicotiana benthamiana</i>. …”
Get full text

FaPOD27 functions in the metabolism of polyphenols in strawberry fruit (Fragaria sp.) by Su-Ying eYeh, Fong-Chin eHuang, Thomas eHoffmann, Mechthild eMayersdorfer, Wilfried eSchwab

“…Moreover, FaPOD27 transcripts were strongly and constitutively induced in fruits exposed to Agrobacterium infection. Gene expression levels and enzymatic activities of FaCCR and FaCAD were efficiently suppressed through RNAi in FaCCR- and FaCAD-silenced strawberries. …”
Get full text

Global Transcriptomic Analysis of Targeted Silencing of Two Paralogous ACC Oxidase Genes in Banana by Yan Xia, Chi Kuan, Chien-Hsiang Chiu, Xiao-Jing Chen, Yi-Yin Do, Pung-Ling Huang

“…To better understand the physiological functions of Mh-ACO1 and Mh-ACO2, two hairpin-type siRNA expression vectors targeting both the Mh-ACO1 and Mh-ACO2 were constructed and incorporated into the banana genome by Agrobacterium-mediated transformation. The generation of Mh-ACO1 and Mh-ACO2 RNAi transgenic banana plants was confirmed by Southern blot analysis. …”
Get full text