A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants by Longzheng Chen, Wei Li, Lorenzo Katin-Grazzini, Jing Ding, Xianbin Gu, Yanjun Li, Tingting Gu, Ren Wang, Xinchun Lin, Ziniu Deng, Richard J. McAvoy, Frederick G. Gmitter, Zhanao Deng, Yunde Zhao, Yi Li

“…Li’s team has used Agrobacterium to transiently express the CRISPR/Cas9 components, a bacterium commonly used to genetically engineer plants. …”
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Protein Extract of Tobacco Expressing <i>Solanum torvum</i> PP5-Encoding Gene Inhibits <i>Verticillium dahliae</i> Proliferation by Min Chen, Chao Xie, Qing Yang, Wenjiao Zhu, Man Wang

“…In order to clarify the role of <i>StPP5</i>, four transgenic tobacco plants expressing <i>StPP5</i> were generated through <i>Agrobacterium</i>-mediated transformation and identified by PCR. …”
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<i>Nicotiana noctiflora</i> Hook. Genome Contains Two Cellular T-DNAs with Functional Genes by Galina V. Khafizova, Nicolas Sierro, Nikolai V. Ivanov, Sofie V. Sokornova, Dmitrii E. Polev, Tatiana V. Matveeva

“…<i>Agrobacterium (Rhizobium)</i>-mediated transformation leads to the formation of crown galls or hairy roots on infected plants. …”
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Ectopic expression of poplar gene PsnERF138 in tobacco confers salt stress tolerance and growth advantages by Lin Liu, Zihan Cheng, Wenjing Yao, Xueyi Wang, Fenglin Jia, Boru Zhou, Tingbo Jiang

“…In this study, PsnERF138 was cloned from Populus alba×Populus glandulosa and transformed into tobacco using the Agrobacterium-mediated transformation method. PsnERF138 was localized in the nucleus through subcellular localization assay in tobacco. …”
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Genome sequencing-assisted identification and the first functional validation of N-acyl-homoserine-lactone synthases from the Sphingomonadaceae family by Han Ming Gan, Lucas K. Dailey, Nigel Halliday, Paul Williams, André O. Hudson, Michael A. Savka

“…Methods In this study, we report the draft genome of the N-(AHL)-producing bacterium Novosphingobium subterraneum DSM 12447 and validate the functions of predicted luxI homologs from the bacterium through inducible heterologous expression in Agrobacterium tumefaciens strain NTL4. We developed a two-dimensional thin layer chromatography bioassay and used LC-ESI MS/MS analyses to separate, detect and identify the AHL signals produced by the N. subterraneum DSM 12447 strain. …”
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Compost Fungi Allow for Effective Dispersal of Putative PGP Bacteria by Susett González-González, Marcia Astorga-Eló, Marco Campos, Lukas Y. Wick, Jacquelinne J. Acuña, Milko A. Jorquera

“…They included the fungal genera <i>Aspergillus, Mucor, Ulocladium, Rhizopus</i> and <i>Syncephalastrum</i>, and the bacterial genera <i>Rhodococcus, Bacillus, Pseudomonas, Agrobacterium, Glutamicibacter</i> and <i>Microbacterium</i>. …”
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Development of CRISPR/Cas9 Construct in Rice (Oryza sativa subsp. indica) Using Golden Gate Cloning Method Towards Drought Tolerance by Nurul Hidayah Samsulrizal, Anis Afuza Md Yusof, Amin-Asyraf Tamizi, Nurul Asyikin Mohd Zim, Siti Syafiqa Abdul Sattar, Mohd Syahmi Salleh, Nur Sabrina Ahmad Azmi, Zamri Zainal, Zarina Zainuddin

“…Further study on the Agrobacterium-mediated transformation using the developed construct may be conducted to determine the efficacy of knocking out candidate genes in improving drought tolerance ability O. sativa…”
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Hiệu quả của vi khuẩn hòa tan lân - kali trên đậu phộng, củ cải trắng và lúa cao sản trồng trên đất cát huyện Tri Tôn, tỉnh An Giang by Nguyễn Thị Dơn, Cao Ngọc Điệp

“…Nghiên cứu được thực hiện nhằm đánh giá hiệu quả ba dòng vi khuẩn hòa tan lân - kali tốt (Agrobacterium tumefaciens CA09, Rhizobium tropici CA29, Azotobacter tropicalis K16B) lên sự tăng trưởng và phát triển của củ cải trắng, đậu phộng và lúa cao sản trên đất cát huyện Tri Tôn, An Giang. …”
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Manipulating resistance to mungbean yellow mosaic virus in greengram (Vigna radiata L): Through CRISPR/Cas9 mediated editing of the viral genome by Ashwini Talakayala, Ashwini Talakayala, Gopala Krishna Mekala, Malireddy K. Reddy, Srinivas Ankanagari, Mallikarjuna Garladinne

“…The cotyledonary nodal explants were infected with Agrobacterium strain EHA105 harboring pMDC100-Cas9 with AC1 and AV1 gRNA cassettes and generated transgenic plants. …”
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Effects of sgRNA length and number on gene editing efficiency and predicted mutations generated in rice by Xiaojing Liu, Jiangtao Yang, Yaya Song, Xiaochun Zhang, Xujing Wang, Zhixing Wang

“…A series of CRISPR-Cas9 plant expression vectors containing single sgRNAs with different lengths (17, 18, 19, 20, 21, 22, 23 nt) or two sgRNAs were constructed and introduced into rice cultivar Zhonghua11 by Agrobacterium-mediated transformation. Analysis of the editing status of 1283 transgenic rice plants showed that 371 were successfully edited with base preference. …”
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THE ACTIVITY OF ESSENTIAL OILS OBTAINED FROM SPECIES AND INTERSPECIES HYBRIDS OF THE Mentha GENUS AGAINST SELECTED PLANT PATHOGENIC BACTERIA by Małgorzata Schollenberger, Tomasz M. Staniek, Elżbieta Paduch-Cichal, Beata Dasiewicz, Agnieszka Gadomska-Gajadhur, Ewa Mirzwa-Mróz

“… Plant essential oils of six aromatic herb species and interspecies hybrids of the family Lamiaceae – chocolate mint (Mentha piperita × ‘Chocolate’), pineapple mint (Mentha suaveolens ‘Variegata’), apple mint (Mentha × rotundifolia), spearmint (Mentha spicata), orange mint (Mentha × piperita ‘Granada’) and strawberry mint (Mentha × villosa ‘Strawberry’) – were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas arboricola pv. corylina. …”
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Aislamiento de la región promotora del gen FaPAL2 de Fragaria x ananassa Cv. “Camino Real” y evaluación de su funcionalidad en respuesta a la irradiación UV-C by Stephannie Masís-Ramos, Jesús Alonso Garduño-Hernández, Edmundo Lozoy- Gloria, Giovanni Garro-Monge

“…“Camino Real” vía Agrobacterium tumefaciens utilizando el gen reportero GUS. …”
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A Pseudomonas syringae pv. tomato avrE1/hopM1 Mutant Is Severely Reduced in Growth and Lesion Formation in Tomato by Jorge L. Badel, Rena Shimizu, Hye-Sook Oh, Alan Collmer

“…AvrE1 expressed from the 35S promoter elicited cell death in nonhost Nicotiana tabacum leaves and host tomato leaves in Agrobacterium-mediated transient expression experiments. …”
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The Variability of Thymol and Carvacrol Contents Reveals the Level of Antibacterial Activity of the Essential Oils from Different Accessions of <i>Oliveria decumbens</i> by Tahereh Khoshbakht, Akbar Karami, Aminallah Tahmasebi, Filippo Maggi

“…In the present study, we analyzed the chemical composition of essential oils obtained from different Iranian populations and evaluated their efficacy on a panel of human pathogens (<i>Staphylococcus aureus</i> and <i>Escherichia coli</i>), probiotic (<i>Bacillus subtilis</i>), and phytopathogens (<i>Clavibacter michiganensis</i>, <i>Curtobacterium flaccumfaciens</i>, <i>Xanthomonas citri</i>, and <i>Agrobacterium tumefaciens</i>). The gas chromatographic-mass spectrometry analysis put in evidence four main volatile constituents such as thymol (20.3–36.4%), carvacrol (18.8–33.1%), γ-terpinene (10.6–25.9%), and <i>p</i>-cymene (9.5–17.3%), though with significant variability from an essential oil to another. …”
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Ecotopic Expression of the Antimicrobial Peptide DmAMP1W Improves Resistance of Transgenic Wheat to Two Diseases: Sharp Eyespot and Common Root Rot by Qiang Su, Ke Wang, Zengyan Zhang

“…<i>DmAMP1W</i> was introduced into a wheat variety Zhoumai18 via <i>Agrobacterium</i>-mediated transformation. The molecular characteristics indicated that <i>DmAMP1W</i> could be heritable and expressed in five transgenic wheat lines in T₁&#8722;T₂ generations. …”
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Efficient transformation of the isolated microspores of Chinese cabbage (Brassica rapa L. ssp. pekinensis) by particle bombardment by Yujia Liu, Shujiang Zhang, Shifan Zhang, Hui Zhang, Guoliang Li, Rifei Sun, Fei Li

“…Particle bombardment is a widely used method along with the Agrobacterium-mediated method. As a physical means, it has almost no restrictions on the type of host and a wide range of receptor types, which largely avoids the restriction of explants. …”
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Genomics of the “tumorigenes” clade of the family Rhizobiaceae and description of Rhizobium rhododendri sp. nov. by Nemanja Kuzmanović, George C. diCenzo, Boyke Bunk, Cathrin Spröer, Anja Frühling, Meina Neumann‐Schaal, Jörg Overmann, Kornelia Smalla

“…Tumorigenic agrobacteria are commonly found in the genera Agrobacterium, Allorhizobium, and Rhizobium. In this study, we analyzed a distinct “tumorigenes” clade of the genus Rhizobium, which includes the tumorigenic species Rhizobium tumorigenes, as well as strains causing crown gall disease on rhododendron. …”
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Subchronic Toxicological Evaluation of Xiushui 134Bt Transgenic Insect-Resistant Rice in Rats by Ruifang Yang, Zhongze Piao, Jianhao Tang, Changzhao Wan, Gangseob Lee, Jianjiang Bai

“…In this study, Xiushui 134Bt, a highly insect-resistant rice transgenic material with stable expression of <i>CryIAc1</i> gene in rice plants and no significant changes in main agronomic traits, was obtained by means of <i>Agrobacterium</i>-mediated transformation method using japonica rice Xiushui 134 as receptor material. …”
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Function Analysis of the <i>PR55</i>/<i>B</i> Gene Related to Self-Incompatibility in Chinese Cabbage Using CRISPR/Cas9 by Na-Ri Shin, Yun-Hee Shin, Han-Seul Kim, Young-Doo Park

“…Tentative gene-edited lines were generated by the <i>Agrobacterium</i>-mediated transfer and were selected by PCR and Southern hybridization analysis. …”
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An inbred line of the diploid strawberry <it>Fragaria vesca </it>f. <it>semperflorens </it>for genomic and molecular genetic studies in the Rosaceae by Folta Kevin M, Schmitt Kyle, Slovin Janet P

“…<it>F. vesca </it>can be transformed with <it>Agrobacterium tumefaciens</it>, making these plants suitable for insertional mutagenesis, RNAi and overexpression studies that can be compared against a stable baseline of phenotypic descriptors and can be readily genetically substantiated.…”
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