Novel Small Antimicrobial Peptides Extracted from Agricultural Wastes Act against Phytopathogens but Not Rhizobacteria by Thitiporn Ditsawanon, Sittiruk Roytrakul, Narumon Phaonakrop, Sawanya Charoenlappanit, Siriwan Thaisakun, Nonglak Parinthawong

“…<i>oryzae</i> via membrane-active mechanisms, while peptide VQLMNSL (Val-Gln-Leu-Met-Asn-Ser-Leu) acted against <i>Pectobacterium carotovorum</i> and <i>Agrobacterium rhizogenes</i> through intracellular-active mechanisms. …”
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A Novel High-Affinity Potassium Transporter <i>IbHKT-like</i> Gene Enhances Low–Potassium Tolerance in Transgenic Roots of Sweet Potato (<i>Ipomoea batatas</i> (L.) Lam.) by Wei Jiang, Rong Jin, Danfeng Wang, Yufeng Yang, Peng Zhao, Ming Liu, Aijun Zhang, Zhonghou Tang

“…<i>IbHKT-like</i> overexpressing transgenic roots were obtained from a sweet potato cultivar Xuzishu8 using an <i>Agrobacterium rhizogenes</i>-mediated root transgenic system in vivo. …”
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Target Lines for <i>in Planta</i> Gene Stacking in <i>Japonica</i> Rice by Ruyu Li, Zhiguo Han, Qian Yin, Meiru Li, Mingyong Zhang, Zhenzhen Li, Ping Wang, Li Jiang, David W. Ow

“…Since this process requires a recombination site in the genome, this work describes the generation of target sites in the <i>Japonica</i> rice genome. <i>Agrobacterium</i>-mediated gene transfer yielded ~4000 random-insertion lines. …”
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An increased proportion of transgenic plants in the progeny of rapeseed (<i>Brassica napus</i> L.) transformants by G. N. Raldugina, T. Z. Hoang, H. B. Ngoc, I. V. Karpichev

“…Cotyledon and leaf explants of two spring rapeseed varieties were transformed with Agrobacterium tumefaciens harboring a genetic construct with the gfp marker gene. …”
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3D‐printed autoclavable plant holders to facilitate large‐scale protein production in plants by Ling Chuang, Anton Enders, Sascha Offermann, Janina Bahnemann, Jakob Franke

“…In this system, gene expression is achieved transiently by infiltrating N. benthamiana plants with suspensions of Agrobacterium tumefaciens carrying vectors with the target genes. …”
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Functional analysis of a susceptibility gene (HIPP27) in the Arabidopsis thaliana-Meloidogyne incognita pathosystem by using a genome editing strategy by Tushar K. Dutta, Neeraj Vashisth, Soham Ray, Victor Phani, Viswanathan Chinnusamy, Anil Sirohi

“…Next, two gRNAs (corresponding to the HIPP27 gene) were artificially synthesized using laboratory protocols, sequentially cloned into a Cas9 editor plasmid, mobilized into Agrobacterium tumefaciens strain GV3101, and transformed into Arabidopsis plants using the floral dip method. …”
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Polygalacturonase-Inhibiting Proteins (PGIPs) with Different Specificities Are Expressed in Phaseolus vulgaris by A. Desiderio, B. Aracri, F. Leckie, B. Mattei, G. Salvi, H. Tigelaar, J. S. C. Van Roekel, D. C. Baulcombe, L. S. Melchers, G. De Lorenzo, F. Cervone

“…Albersheim, Plant J. 2:367–373, 1992), was expressed under control of the cauliflower mosaic virus 35S promoter in tomato plants via Agrobacterium tumefaciens-mediated transformation. Transgenic tomato plants with different expression levels of PGIP-1 were used in infection experiments with the pathogenic fungi Fusarium oxysporum f. sp. lycopersici, Botrytis cinerea, and Alternaria solani. …”
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The replication origin of a repABC plasmid by Cevallos Miguel A, Pérez-Segura Gabriela, Pedraza-López Francisco, Cervantes-Rivera Ramón

“…The minimal replicon could not be introduced into <it>R. etli </it>strain containing p42d, but similar constructs that carried <it>repC </it>from <it>Sinorhizobium meliloti </it>pSymA or the linear chromosome of <it>Agrobacterium tumefaciens </it>replicated in the presence or absence of p42d, indicating that RepC is an incompatibility factor. …”
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Functional Characterization of the Wheat Macrophage Migration Inhibitory Factor TaMIF1 in Wheat-Stripe Rust (<i>Puccinia striiformis</i>) Interaction by Mengxin Zhao, Qing Chang, Yueni Liu, Peng Sang, Zhensheng Kang, Xiaojie Wang

“…Additionally, <i>Ta</i>MIF1 possessed significant tautomerase activity, indicating conservation of MIFs across kingdoms. <i>Agrobacterium tumefaciens</i> infiltration assay demonstrated that <i>Ta</i>MIF1 was capable of suppressing programmed cell death hinting its role in plant immunity. …”
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Transformation and expressional studies of GaZnF gene to improve drought tolerance in Gossypium hirsutum by Fatima Batool, Sameera Hassan, Saira Azam, Zunaira Sher, Qurban Ali, Bushra Rashid

“…CIM-482, a local Gossypium hirsutum variety was transformed with GaZnF through Agrobacterium-mediated transformation method with 2.57% transformation efficiency. …”
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Caracterización de bioinsumos producidos artesanalmente en Nicaragua by Johana O'Connor-Mendoza, Leandro Páramo-Aguilera, Griselda Martínez-Laguna, Laura Guillén-Rodríguez

“…Zona de occidente, muestra DCL: 9 bacterias (2 Lysinibacillus macroides, Bacillus subtilis, Bacillus flexus, Bacillus cereus – patógeno para el ser humano, Agrobacterium tumefaciens, 2 Bacillus sp. y una Stenotrophomonas sp.) y 1 hongo levaduriforme no identificado molecularmente. …”
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Silencing of <i>D</i><i>ihydroflavonol 4-reductase</i> in Chrysanthemum Ray Florets Enhances Flavonoid Biosynthesis and Antioxidant Capacity by Sun-Hyung Lim, Da-Hye Kim, Jae-A Jung, Nam-In Hyung, YeoJin Youn, Jong-Yeol Lee

“…Transgenic <i>CmDFR</i>-RNAi chrysanthemum plants were obtained by <i>Agrobacterium</i>-mediated transformation. Genomic PCR analysis of <i>CmDFR</i>-RNAi chrysanthemums propagated by several rounds of stem cuttings verified stable transgene integration into the genome. …”
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Hypothetical protein FoDbp40 influences the growth and virulence of Fusarium oxysporum by regulating the expression of isocitrate lyase by Busi Zhao, Dan He, Song Gao, Yan Zhang, Li Wang

“…A mutant of F. oxysporum with reduced growth was obtained by Agrobacterium tumefaciens-mediated transformation, the transferred DNA (T-DNA) interrupted gene in this mutant coded a hypothetical protein that we named FoDbp40. …”
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Accumulation of colicin M protein and its biological activity in transgenic lettuce and mizuna plants by Nataliia Shcherbak, Heike Prochaska, Kateryna Lystvan, Yelizaveta Prokhorova, Anatoli Giritch, Mykola Kuchuk

“…Transgenic plants of these species were developed using Agrobacterium-mediated transformation with a vector containing the ColM-coding gene (cma) under the control of the 35S promoter. …”
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Development of sorghum mutants with improved in vitro protein digestibility by CRISPR/Cas9 editing of kafirin genes by Lev A. Elkonin, Grigoriy A. Gerashchenkov, Natalie V. Borisenko, Odyssey A. Kenzhegulov, Saule Kh. Sarsenova, Natalya A. Rozhnova, Valery M. Panin

“…To induce mutations in kafirin-encoding genes that were expected to disturb their accumulation in endosperm cells, we used a genome-editing approach. By Agrobacterium-mediated genetic transformation of immature embryos of cv. …”
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Functional analysis of ARF1 from Cymbidium goeringii in IAA response during leaf development by Zihan Xu, Fangle Li, Meng Li, Yuanhao He, Yue Chen, Fengrong Hu

“…The CgARF1 gene was overexpressed in wild-type Arabidopsis and Nicotiana benthamiana via the Agrobacterium infection method. Acetone-ethanol solvent extraction was applied for the determination of chlorophyll contents, and the contents of endogenous hormones were determined using the enzyme-linked immunosorbent assay technique. …”
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Classifying Interactions in a Synthetic Bacterial Community Is Hindered by Inhibitory Growth Medium by Andrea R. Dos Santos, Rita Di Martino, Samuele E. A. Testa, Sara Mitri

“…Here, we performed spent medium assays based on the predictions of a mathematical model to decipher the interactions among four bacterial species: Agrobacterium tumefaciens, Comamonas testosteroni, Microbacterium saperdae, and Ochrobactrum anthropi. …”
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A nematode demographics assay in transgenic roots reveals no significant impacts of the <it>Rhg1 </it>locus LRR-Kinase on soybean cyst nematode resistance by Diers Brian W, Cook David, Heuberger Adam L, Melito Sara, MacGuidwin Ann E, Bent Andrew F

“…Generation of fertile transgenic soybean lines is difficult but methods have been published that test SCN resistance in transgenic roots generated with <it>Agrobacterium rhizogenes</it>.</p> <p>Results</p> <p>We report use of artificial microRNA (amiRNA) for gene silencing in soybean, refinements to transgenic root SCN resistance assays, and functional tests of the <it>Rhg1 </it>locus LRR-kinase gene. …”
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Examination of the nematostella vectensis holobiont by comparative bacterial genomics and metatranscriptomics by Helbig, Timothy J. (Timothy James)

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Functional analysis of a latex-specific promoter (hevein) of Hevea for enhanced recombinant protein production in Hevea brasiliensis by Pappusamy, Arokiaraj / PA, Shuib, Siti Shuhada, Enamalai, Sunderasan

“…The expression vectors containing the hevein promoter fused to uidA in Agrobacterium tumefaciens GV2260 were then used to infect Hevea anther callus. …”
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