GRAS family transcription factor FaSCL8 regulates FaVPT1 expression mediating phosphate accumulation and strawberry fruit ripening by Zhenzhen Zheng, Yanjun Zhang, Yongqiang Gao, Yuanyue Shen, Yun Huang

“…FaSCL8 can directly combine with the FaVPT1 promoter and regulate its expression in vitro and in vivo. The results of Agrobacterium-mediated transient infection of strawberry fruits showed that overexpression of FaSCL8 could increase soluble sugar, anthocyanin, and phosphorus content, promoting fruit ripening, while decreasing expression of FaSCL8 showed the opposite phenotype. …”
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Systematic Characterization of bZIP Transcription Factors Required for Development and Aflatoxin Generation by High-Throughput Gene Knockout in <i>Aspergillus flavus</i> by Qianqian Zhao, Hao Pei, Xiaoling Zhou, Kai Zhao, Min Yu, Guomin Han, Jun Fan, Fang Tao

“…In this study, we developed a high-throughput gene knockout method based on an <i>Agrobacterium</i>-mediated transformation system. Gene knockout construction by yeast recombinational cloning and screening of the null mutants by double fluorescence provides an efficient way to construct gene-deleted mutants for this multinucleate fungus. …”
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Plant Peroxisome-Targeting Effector MoPtep1 Is Required for the Virulence of <i>Magnaporthe oryzae</i> by Na Ning, Xin Xie, Haiyue Yu, Jie Mei, Qianqian Li, Shimin Zuo, Hanxiang Wu, Wende Liu, Zhiqiang Li

“…In our previous study, we analyzed the subcellular localization of candidate effectors from <i>M. oryzae</i> using the agrobacterium-mediated transient expression system in tobacco and found that MoPtep1 (peroxisomes-targeted effector protein 1) localized in plant peroxisomes. …”
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Harnessing a Transient Gene Expression System in <i>Nicotiana benthamiana</i> to Explore Plant Agrochemical Transporters by Bingqi Wu, Zhiting Chen, Xiaohui Xu, Ronghua Chen, Siwei Wang, Hanhong Xu, Fei Lin

“…Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. <i>Agrobacterium</i>-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. …”
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Phenolic signals for prehaustorium formation in Striga hermonthica by Natsumi Aoki, Songkui Cui, Songkui Cui, Chiharu Ito, Kie Kumaishi, Shungo Kobori, Yasunori Ichihashi, Satoko Yoshida

“…We found that ASBr, an inhibitor of acetosyringone in virulence gene induction in the soil bacterium Agrobacterium, compromised prehaustorium formation in S. hermonthica. …”
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The transcription factor Ste12-like increases the mycelial abiotic stress tolerance and regulates the fruiting body development of Flammulina filiformis by Xiaomeng Lyu, Xiaomeng Lyu, Qingji Wang, Ao Liu, Fang Liu, Li Meng, Panmeng Wang, Yan Zhang, Li Wang, Zhuang Li, Wei Wang

“…Four ste12-like overexpression transformants of F. filiformis were constructed by Agrobacterium tumefaciens-mediated transformation.Results and DiscussionPhylogenetic analysis showed that Ste12-like contained conserved amino acid sequences. …”
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Physicochemical and Rheological Properties of Succinoglycan Overproduced by <i>Sinorhizobium meliloti</i> 1021 Mutant by Jaeyul Kim, Jae-pil Jeong, Yohan Kim, Seunho Jung

“…Succinoglycan (SG) is a type of bacterial anionic exopolysaccharide produced by <i>Rhizobium</i>, <i>Agrobacterium</i>, and other soil bacterial species. …”
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Development of patatin knockdown potato tubers using RNA interference (RNAi) technology, for the production of human-therapeutic glycoproteins by Ko Jeong-Heon, Hahn Kyu-Woong, Kim Mi-Sun, Kim Hyun-Soon, Lee Yong-Hwa, Kim Yoon-Sik, Joung Hyouk, Jeon Jae-Heung

“…The CaMV 35S promoter-driven patatin hpRNAi vector was transformed into the potato cultivar Desiree by <it>Agrobacterium</it>-mediated transformation. Ten transgenic potato lines bearing patatin hpRNA were generated. …”
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Manifestation of agronomically valuable traits in the progeny of a sorghum mutant carrying the genetic construct for RNA silencing of the γ-kafirin gene by L. A. Elkonin, N. V. Borisenko, T. E. Pylaev, O. A. Kenzhegulov, S. Kh. Sarsenova, N. Yu. Selivanov, V. M. Panin

“…The introduction of genetic constructs capable of inducing RNA silencing of the γ-kafirin (gKAF1) gene opens up prospects for solving this problem. Using Agrobacterium-mediated genetic transformation of immature embryos of the grain sorghum cv. …”
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TẠO DÒNG CÁC GEN MÃ HÓA CHITINASE 42 kDa CỦA Trichoderma asperellum VÀO VECTOR BIỂU HIỆN THỰC VẬT pMYV719 ĐỂ PHỤC VỤ CHUYỂN GEN by Thị Bích Hoà Phung, Hoang Tue Nguyen, Thi Huyen Trang Pham, Gia Cat Tuong Tran, Thi Huyen Trang Huynh, Xuan Huy Nguyen, Hoang Loc Nguyen

“… Trong nghiên cứu này, các gen chitinase mang trình tự peptide tín hiệu như Chi42, syncodChi42-1 và syncodChi42-2 đã được tạo dòng trong vector biểu hiện thực vật pMYV719 và biến nạp thành công vào vi khuẩn Agrobacterium tumefaciens LBA 4404. Trong đó, gen Chi42 là kiểu gen hoang dại từ chủng nấm Trichoderma asperellum SH16. …”
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Characterization of Pathogenicity-Associated V2 Protein of Tobacco Curly Shoot Virus by Mingjun Li, Changchang Li, Kairong Jiang, Ke Li, Junlei Zhang, Miao Sun, Gentu Wu, Ling Qing

“…In our report, an <i>Agrobacterium</i> infiltration-mediated transient expression assay indicated that TbCSV V2 can suppress local and systemic RNA silencing and the deletion analyses demonstrated that the amino acid region 1–92 of V2, including the five predicted α-helices, are required for local RNA silencing suppression. …”
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Generation of Iron-Independent Siderophore-Producing <i>Agaricus bisporus</i> through the Constitutive Expression of <i>hapX</i> by Min-Seek Kim, Hyeon-Su Ro

“…Transformants of <i>A. bisporus</i> were generated using the binary vector through <i>Agrobacterium tumefaciens</i>-mediated transformation. …”
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Genotype-Dependent Effect of Silencing of <i>TaCKX1</i> and <i>TaCKX2</i> on Phytohormone Crosstalk and Yield-Related Traits in Wheat by Bartosz Jablonski, Andrzej Bajguz, Joanna Bocian, Waclaw Orczyk, Anna Nadolska-Orczyk

“…<i>TaCKX</i> GFM expression, phytohormone metabolism and phenotype were additionally modified by <i>Agrobacterium</i>-mediated transformation. Two novel phytohormones, phenylacetic acid (PAA) and topolins, lack of gibberellic acid (GA) and changed phytohormone contents in the 7 days after pollination (DAP) spikes of the awned-spike cultivar compared to a previously tested, awnless one, were detected. …”
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Phylogenetic and functional analysis of tiller angle control homeologs in allotetraploid cotton by Foster Kangben, Sonika Kumar, Zhigang Li, Avinash Sreedasyam, Chris Dardick, Don Jones, Christopher A. Saski

“…To silence highly expressed specific TAC1 homeologs in the stem, we applied CRISPR-Cas9 gene editing and Agrobacterium-mediated transformation, followed by genotyping and subsequent phenotypic validation of the mutants.ResultsGene duplication events of TAC1 specific to the Gossypium lineage were identified, with 3 copies in diploid progenitors and 6 copies in allotetraploid cottons. …”
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Impact of intercropping grass on the soil rhizosphere microbial community and soil ecosystem function in a walnut orchard by Changxi Wang, Qiang Liang, Qiang Liang, Jianning Liu, Rui Zhou, Xinya Lang, Shengyi Xu, Xichen Li, Andi Gong, Yutian Mu, Hongcheng Fang, Hongcheng Fang, Ke Qiang Yang, Ke Qiang Yang

“…In addition, we found that the soil microorganisms of walnut/Vv intercropping had a higher potential for nitrogen cycling and carbohydrate metabolism, which may be related to the functions of Burkholderia, Rhodopseudomonas, Pseudomonas, Agrobacterium, Paraburkholderia, and Flavobacterium. …”
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Genetic Transformation of Potato without Antibiotic-Assisted Selection by Dmitry Miroshnichenko, Anna Klementyeva, Tatiana Sidorova, Alexander S. Pushin, Sergey Dolgov

“…Internodal segments of in vitro potato plants were inoculated with the <i>Agrobacterium</i> strain AGL0 carrying plasmids, which contained the <i>GFP</i> or <i>RFP</i> gene driven by the <i>CaMV 35S</i> promoter to monitor the transformation process by observing in vivo green or red fluorescence. …”
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Specific gastrointestinal microbiota profiles in Chinese Tan sheep are associated with lauric acid content in muscle by Zhen LI, Ran Cui, Yu-Bei Wang, Ya-Biao Luo, Peng-Xiang Xue, Qi-Guo Tang, Mei-Ying Fang

“…In the ruminal, duodenal, and colonic microbiome, several bacteria were uniquely identified in respective breeds, including Agrobacterium tumefaciens, Bacteroidales bacterium CF, and several members of the family Oscillospiraceae. …”
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The sulfoquinovosyl glycerol binding protein SmoF binds and accommodates plant sulfolipids by Alexander J.D. Snow, Mahima Sharma, James P. Lingford, Yunyang Zhang, Janice W.-Y. Mui, Ruwan Epa, Ethan D. Goddard-Borger, Spencer J. Williams, Gideon J. Davies

“…The recently discovered sulfoglycolytic sulfoquinovose monooxygenase (sulfo-SMO) pathway in Agrobacterium tumefaciens features a periplasmic sulfoquinovosyl glycerol binding protein, SmoF, and an ATP-binding cassette (ABC) transporter. …”
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Diversity, Phylogeny and Plant Growth Promotion Traits of Nodule Associated Bacteria Isolated from <i>Lotus parviflorus</i> by Ricardo Soares, Jesús Trejo, Maria J. Lorite, Etelvina Figueira, Juan Sanjuán, Isabel Videira e Castro

“…The non-nodulating bacteria isolated were alpha- (<i>Rhizobium/Agrobacterium</i>), beta- (<i>Massilia</i>) and gamma-proteobacteria (<i>Pseudomonas</i>, <i>Lysobacter, Luteibacter</i>, <i>Stenotrophomonas</i> and <i>Rahnella</i>), as well as some bacteroidetes from genera <i>Sphingobacterium</i> and <i>Mucilaginibacte</i>r. …”
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<i>Polyscias filicifolia</i> (<i>Araliaceae</i>) Hairy Roots with Antigenotoxic and Anti-Photogenotoxic Activity by Anita Śliwińska, Ramona Figat, Anna Zgadzaj, Beata Wileńska, Aleksandra Misicka, Grzegorz Nałęcz-Jawecki, Agnieszka Pietrosiuk, Katarzyna Sykłowska-Baranek

“…The transformation with <i>Agrobacterium rhizogenes</i> ATCC 15834 allowed to obtain 15 root lines. …”
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