Optimización de condiciones de cultivo de tejidos y transformación in vitro e in planta de la variedad local de arroz Don Justo FCAyF by Martín Andrés Duhalde, Rodolfo Bezus, Andrés Alberto Rodriguez, Santiago Javier Maiale, Fernando Matias Romero

“…En relación a la transformación mediante Agrobacterium tumefaciens se obtuvieron diferentes porcentajes de eficiencia dependiendo del plásmido utilizado para transformar como también del explanto. …”
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Improvement of a Genetic Transformation System and Preliminary Study on the Function of <i>LpABCB21</i> and <i>LpPILS7</i> Based on Somatic Embryogenesis in <i>Lilium pumilum</i> D... by Shengli Song, Rui Yan, Chunxia Wang, Jinxia Wang, Hongmei Sun

“…Auxin transport mediates the asymmetric distribution of auxin that determines the fate of cell development. <i>Agrobacterium</i>-mediated genetic transformation is an important technical means to study gene function. …”
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Generation of Asynaptic Mutants in Potato by Disrupting <i>StDMC1</i> Gene Using RNA Interference Approach by Ashwani Kumar, Sundaresha Siddappa, Vinay Bhardwaj, Dalamu, Baljeet Singh, Neha Sharma, Bhawna Dipta, Vinod Kumar, Umesh Goutam, Salej Sood

“…The sense and antisense fragments were confirmed by Sanger-sequencing and cloned in the pRI101 vector. <i>Agrobacterium</i>-mediated transformation of the RNAi construct resulted in 44% transformation efficiency, and a total of 137 mutant lines were obtained. …”
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CRISPR/Cas9-mediated genome editing of RsGL1a and RsGL1b in radish (Raphanus sativus L.) by Naoki Muto, Takashi Matsumoto

“…This study aimed to establish a CRISPR/Cas9 system in radish employing the Agrobacterium-mediated genetic transformation system reported recently. …”
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A simple and effective VIGS system facilitates the control of citrus canker by silencing CsLOB1 by Chunqing Wang, Zhimin Ma, Jinhuan Zhou, Jiaxin Li, Peng Cao, Chenhu Song, Chuxin Li, Xinliang Wang, Yu Bin, Changyong Zhou, Zhen Song

“…Here, we report the development of a simple and effective VIGS system based on citrus leaf blotch virus (CLBV), a virus endemic in most citrus-producing countries but showing no symptoms in most citrus varieties. By Agrobacterium-mediated vacuum-infiltration (AVI), pCLBV201-su245 and pCLBV201-pds391 triggered efficient gene silencing (85%) and silencing phenotypes began to appear at 30 days post infiltration/inoculation (dpi), a similar period for achieving that on herbaceous plants. …”
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CRISPR/Cas9-mediated disruption of CjACOS5 confers no-pollen formation on sugi trees (Cryptomeria japonica D. Don) by Mitsuru Nishiguchi, Norihiro Futamura, Masaki Endo, Masafumi Mikami, Seiichi Toki, Shin-Ichiro Katahata, Yasunori Ohmiya, Ken-ichi Konagaya, Yoshihiko Nanasato, Toru Taniguchi, Tsuyoshi Emilio Maruyama

“…As a result of sugi transformation mediated by Agrobacterium tumefaciens harboring the CjACOS5-targeted CRISPR/Cas9 vector, 1 bp-deleted homo biallelic mutant lines were obtained. …”
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Genetic Transformation of <i>Quercus ilex</i> Somatic Embryos with a Gnk2-like Protein That Reveals a Putative Anti-Oomycete Action by Susana Serrazina, Mª Teresa Martínez, Vanesa Cano, Rui Malhó, Rita Lourenço Costa, Elena Corredoira

“…PEMs were co-cultured for 5 days with <i>Agrobacterium</i> EHA105pGnk2 and then cultured on selective medium containing kanamycin (kan) and carbenicillin. …”
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KARAKTERISASI ISOLAT BAKTERI PEMBINTIL AKAR LEGUM DARI BEBERAPA AGROEKOSISTEM DI LAMPUNG BARAT by , PRENALY .A. AMALIA, , Ir. Sri Wedhastri, M.S.

“…The results of root nodules formation test, there were 3 isolates, Rhizobium sp. TANU 14/ Agrobacterium veiscosum, Pseudomonas stutzeri, and Azospira could form nodules on 6 legumes as well as Bradyrhizobium japonicum USDA122 and THA7. …”

Protein domains and architectural innovation in plant-associated Proteobacteria. by Studholme, D, Downie, J, Preston, G

“…One example is the RolB/RolC glucosidase family, which is found only in Agrobacterium species and in plants. We identified the 0.5% of Pfam protein domain families that were most significantly over-represented in the plant-associated Proteobacteria with respect to the background frequencies in the whole set of available proteobacterial proteomes. …”

Protein domains and architectural innovation in plant-associated Proteobacteria

“…One example is the RolB/RolC glucosidase family, which is found only in Agrobacterium species and in plants. We identified the 0.5% of Pfam protein domain families that were most significantly over-represented in the plantassociated Proteobacteria with respect to the background frequencies in the whole set of available proteobacterial proteomes. …”

Casual agent of ginger bacterial wilt in Sabah and effect of Brassica juncea var. rugosa as its control by Linda @ Lily Cosmas

“…MALDI-TOF analysis identified six species of the isolates as Enterobacter cloacae complex (57.9%), Ralstonia pickettii (10.5%), Agrobacterium tumefaciens (10.5%), Bac11/us pumilus (10.5%), Stenotrophomonas maltophilia (5.3%) and Serratia marcescens (5.3%). …”
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Improvement in Organogenesis and the Development of a Transformation Procedure for Cucumber and Muskmelon by Mohi Uddin, A. K. M.

“…The studies carried out were: (i) a comparative study on direct and indirect shoot regeneration (ii) the improvement of a direct shoot regeneration system by using an ethylene action inhibitor, silver nitrate and an ethylene biosynthesis inhibitor, cobalt chloride (iii) identification of suitable strains of Agrobacterium tumefaciens and A. rhizogenes that are able to infect cucumber and muskmelon plants (iv) development of an intron containing gene transformation system for both cucurbit species through A. tumefaciens and (v) analysis of transformants. …”
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Dose immunogenicity study of a plant-produced influenza virus-like particle vaccine in layer hens by Celia Abolnik, Tanja Smith, Daniel B.R. Wandrag, Mark-Anthony Murphy, Marizel Rautenbach, Olebile Olibile, Martha O'Kennedy

“…The antigenic match between a vaccine and the circulating field influenza A viruses (IAV) is a critical determinant of vaccine efficacy. Here, an Agrobacterium tumefaciens-mediated transient tobacco plant (Nicotiana benthamiana) system was used to rapidly update an H6 influenza subtype virus-like particle (VLP) vaccine expressing the hemagglutininn (HA) protein of South African H6N2 IAVs circulating in 2020. …”
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Effect of leaf position and days post-infiltration on transient expression of colorectal cancer vaccine candidate proteins GA733-Fc and GA733-FcK in Nicotiana benthamiana plant by Kibum Kim, Yang Joo Kang, Se Ra Park, Do-Sun Kim, Seung-Won Lee, Kinarm Ko, Daniel Ponndorf, Kisung Ko

“…We cloned a fusion gene of GA733 and immunoglobulin Fc fragment (GA733-Fc), and that of GA733-Fc and an endoplasmic reticulum retention motif (GA733-FcK) into the Cowpea mosaic virus (CPMV)-based transient plant expression vector, pEAQ-HT. Agrobacterium tumefaciens (LBA4404) transformed with the vectors pEAQ-HT-GA733-Fc and pEAQ-HT-GA733-FcK was infiltrated into the leaves of Nicotiana benthamiana plants. …”
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Identification of the Function of the Pathogenesis-Related Protein GmPR1L in the Resistance of Soybean to <i>Cercospora sojina</i> Hara by Yeyao Du, Nooral Amin, Naveed Ahmad, Hanzhu Zhang, Ye Zhang, Yang Song, Sujie Fan, Piwu Wang

“…<i>GmPR1L</i> was either overexpressed or silenced in soybean seedlings through <i>Agrobacterium</i>-mediated transformation to examine the resistance of soybean to infection caused by <i>Cercospora sojina</i> Hara. …”
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Candidate Effectors from <i>Botryosphaeria dothidea</i> Suppress Plant Immunity and Contribute to Virulence by Chuan-Jie Zhang, Shi-Xing Wang, Yan-Na Liang, Sheng-Hui Wen, Bao-Zhu Dong, Zheng Ding, Li-Yun Guo, Xiao-Qiong Zhu

“…Twenty-four hours after initial inoculation with <i>Agrobacterium tumefaciens</i> cells carrying candidate effectors, the infiltrated leaves were challenged with <i>A. tumefaciens</i> cells carrying the <i>BAX</i> gene. …”
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High-efficiency fungal pathogen intervention for seed protection: new utility of long-chain alkyl gallates as heat-sensitizing agents by Jong H. Kim, Kathleen L. Chan, William M. Hart-Cooper, Jeffrey D. Palumbo, William J. Orts

“…Heat-sensitization was also determined in selected bacterial strains (Escherichia coli, Agrobacterium tumefaciens). Altogether, OG is an effective heat-sensitizing agent for control of microbial pathogens. …”
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Glucoraphanin and sulforaphane biosynthesis by melatonin mediating nitric oxide in hairy roots of broccoli (Brassica oleracea L. var. italica Planch): insights from transcriptome d... by Shaoying Ma, Jinyu Bao, Yaqi Lu, Xu Lu, Peng Tian, Xiaoling Zhang, Jie Yang, Xiaotong Shi, Zhihui Pu, Sheng Li

“…Italic Planch) leaves with Agrobacterium rhizogenes (ATCC15834) are phytohormonally autonomous, genetically stable, and can produce large amounts of the anti-cancer substance SF. …”
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Design of β-Keto Esters with Antibacterial Activity: Synthesis, In Vitro Evaluation, and Theoretical Assessment of Their Reactivity and Quorum-Sensing Inhibition Capacity by Maximiliano Martínez-Cifuentes, Emmanuel Soto-Tapia, Camila Linares-Pipón, Ben Bradshaw, Paulina Valenzuela-Hormazabal, David Ramírez, Patricio Muñoz-Torres, Claudio Parra

“…An initial in vitro antimicrobial screening was performed against the human pathogenic bacteria <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> as well as the phytopathogenic bacteria <i>Pseudomonas syringae</i> and <i>Agrobacterium tumefaciens</i>. Compounds <b>6</b> and <b>8</b> exhibit the most promising results in the in vitro antimicrobial screening against the panel of bacteria studied.…”
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AtCIPK16, a CBL-interacting protein kinase gene, confers salinity tolerance in transgenic wheat by Khadija Imtiaz, Moddassir Ahmed, Nazish Annum, Mark Tester, Nasir A. Saeed

“…In this study, the AtCIPK16 gene was cloned in two different plant expression vectors, i.e., pTOOL37 having a UBI1 promoter and pMDC32 having a 2XCaMV35S constitutive promoter transformed through the Agrobacterium-mediated transformation protocol, in the local wheat cultivar Faisalabad-2008. …”
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