Unraveling the Diverse Profile of N-Acyl Homoserine Lactone Signals and Their Role in the Regulation of Biofilm Formation in <i>Porphyra haitanensis</i>-Associated <i>Pseudoalterom... by Muhammad Aslam, Pengbing Pei, Peilin Ye, Tangcheng Li, Honghao Liang, Zezhi Zhang, Xiao Ke, Weizhou Chen, Hong Du

“…The bacterium isolated from the surface of <i>P. haitanensis</i> was identified as <i>Pseudoalteromonas galatheae</i> by 16s rRNA gene sequencing and screened for AHLs using two AHL reporter strains, <i>Agrobacterium tumefaciens</i> A136 and <i>Chromobacterium violaceum</i> CV026. …”
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A Nickel-Containing Polyoxomolybdate as an Efficient Antibacterial Agent for Water Treatment by Jiangnan Chang, Mingxue Li, Jiyuan Du, Min Ma, Cuili Xing, Lin Sun, Pengtao Ma

“…<b>1</b> demonstrated significant antibacterial activity, with minimum inhibitory concentration (MIC) values in the range of 0.0019–0.2400 µg/mL against four types of bacteria, including <i>Staphylococcus aureus</i> (<i>S. aureus</i>), <i>Bacillus subtilis</i> (<i>B. subtilis</i>), <i>Escherichia coli</i> (<i>E. coli</i>), and <i>Agrobacterium tumefaciens</i> (<i>A. tumefaciens</i>). …”
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Two genes, ANS and UFGT2, from Vaccinium spp. are key steps for modulating anthocyanin production by Han M. Nguyen, Han M. Nguyen, Joanna Putterill, Andrew P. Dare, Blue J. Plunkett, Janine Cooney, Yongyan Peng, Edwige J. F. Souleyre, Nick W. Albert, Richard V. Espley, Catrin S. Günther

“…We isolated three candidate genes, CHALCONE SYNTHASE (VmCHS1), ANTHOCYANIDIN SYNTHASE (VmANS) and UDP-GLUCOSE : FLAVONOID-3-O-GLYCOSYLTRANSFERASE (VcUFGT2), from Vaccinium, which were predominantly expressed in pigmented fruit skin tissue and showed high homology between bilberry and blueberry. Agrobacterium-mediated transient expression of Nicotiana benthamiana showed that overexpression of VcMYBA1 in combination with VmANS significantly increased anthocyanin concentration (3-fold). …”
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Genes with Cold Shock Domain from <i>Eutrema salsugineum</i> (Pall.) for Generating a Cold Stress Tolerance in Winter Rape (<i>Brassica napus</i> L.) Plants by Pavel Khvatkov, Vasiliy Taranov, Alexander Pushin, Galina Maletich, Vladislav Fedorov, Inna Chaban, Alexsey Babakov, Sergey Dolgov, Mariya Chernobrovkina

“…Nine transgenic lines of winter rape containing genes encoding proteins with a cold shock domain (CspA и EsCSDP3) were obtained as a result of <i>Agrobacterium</i> transformation. In total, 260 explants were involved in transformation of rape using pBI121-CSPA-plant, with a transformation efficiency of 2.3%; among 750 explants using the pBI-EsCSDP3 construction, the efficiency was 0.4%. …”
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Transcriptome-Wide Integrated Analysis of the <i>PgGT25-04</i> Gene in Controlling Ginsenoside Biosynthesis in <i>Panax ginseng</i> by Lei Zhu, Jian Hu, Ruiqi Li, Chang Liu, Yang Jiang, Tao Liu, Mingming Liu, Mingzhu Zhao, Yi Wang, Kangyu Wang, Meiping Zhang

“…The interfering vector pBI121-PgGT25-04 containing the <i>PgGT25-04</i> gene was constructed, and the ginseng adventitious roots were transformed using the Agrobacterium-mediated method to obtain the pBI121-PgGT25-04 positive hairy root monocot line. …”
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A Versatile Peroxidase from the Fungus <i>Bjerkandera adusta</i> Confers Abiotic Stress Tolerance in Transgenic Tobacco Plants by Nancy Sofia Hernández-Bueno, Ramón Suárez-Rodríguez, Edgar Balcázar-López, Jorge Luis Folch-Mallol, José Augusto Ramírez-Trujillo, Gabriel Iturriaga

“…We cloned the <i>VP</i> gene and expressed it via the <i>Agrobacterium</i> transformation procedure in transgenic tobacco plants to assay their tolerance to different abiotic stress conditions. …”
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VvEPFL9-1 Knock-Out via CRISPR/Cas9 Reduces Stomatal Density in Grapevine by Molly Clemens, Molly Clemens, Molly Clemens, Michele Faralli, Jorge Lagreze, Luana Bontempo, Stefano Piazza, Claudio Varotto, Mickael Malnoy, Walter Oechel, Walter Oechel, Annapaola Rizzoli, Lorenza Dalla Costa

“…A set of plants, regenerated after gene transfer in embryogenic calli via Agrobacterium tumefaciens, were selected for evaluation. …”
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Transgene-Free Genome Editing for Biotic and Abiotic Stress Resistance in Sugarcane: Prospects and Challenges by Sakthivel Surya Krishna, S R Harish Chandar, Maruthachalam Ravi, Ramanathan Valarmathi, Kasirajan Lakshmi, Perumal Thirugnanasambandam Prathima, Ramaswamy Manimekalai, Rasappa Viswanathan, Govindkurup Hemaprabha, Chinnaswamy Appunu

“…Transgene-free genome editing approaches, such as delivery of the RNPs through biolistics or protoplast fusion, virus-induced genome editing (VIGE), transient expression of CRISPR/Cas reagents through <i>Agrobacterium</i>-mediated transformation and other approaches, are discussed. …”
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Investigation of Potential In Vitro Anticancer and Antimicrobial Activities of <i>Balanites aegyptiaca</i> (L.) Delile Fruit Extract and Its Phytochemical Components by Omer H. M. Ibrahim, Adel D. Al-Qurashi, Khalid A. Asiry, Magdi A. A. Mousa, Nabil A. Alhakamy, Kamal A. M. Abo-Elyousr

“…The calculated minimum inhibitory concentration (MIC) was similar (62.5 µg/mL) against the three tested bacterial strains (<i>Acinetobacter johnsonii</i>, <i>Serratia marcescens</i> and <i>Agrobacterium tumefaciens</i>), while it was higher than 1000 µg/mL for the fungal species (<i>Rhizoctonia solani</i>, <i>Penicillium italicum</i>, and <i>Fusarium oxysporium</i>). …”
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The Amplification and Activity Analysis of the Small Rubber Particle Protein (<i>SRPP</i>) Promoter of <i>Eucommia ulmoides</i> (<i>EuSRPP</i>) Revealed That Its Activity Was Regul... by Huimin Zhao, Xinzhuan Yao, Litang Lu

“…Then, the T3 generation of the transgenic homozygous line was obtained via a genetic transformation of <i>Arabidopsis thaliana</i> mediated by Agrobacterium. The six <i>EuSRPP</i> promoters were expressed in transgenic plants and were stably expressed in the leaves, pollinated flowers, and mature pods. …”
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Using A Protoplast Transformation System to Enable Functional Studies in <i>Mangifera indica</i> L. by Mark Owusu Adjei, Huan Zhao, Xiaoguang Tao, Li Yang, Shuyue Deng, Xiyan Li, Xinjing Mao, Shujiang Li, Jianfeng Huang, Ruixiong Luo, Aiping Gao, Jun Ma

“…The protoplast technology is an excellent method for creating a rapid and effective tool for transient expression and transformation assays, particularly in plants that lack an Agrobacterium-mediated plant transformation system. This study optimized the conditions of the protoplast isolation and transformation system, which can provide a lot of help in the gene expression regulation study of mango. …”
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Multifaceted Role of Nanomaterials in Modulating In Vitro Seed Germination, Plant Morphogenesis, Metabolism and Genetic Engineering by Ashutosh Pathak, Shamshadul Haq, Neelam Meena, Pratibha Dwivedi, Shanker Lal Kothari, Sumita Kachhwaha

“…Recent advancements in this field also depict the suitability of nanoparticles as a promising carrier for gene transfer, which show better efficiency than traditional <i>Agrobacterium</i>-mediated delivery. This review comprehensively highlights different in vitro studies that will aid in identifying research gaps and provide future directions for unexplored areas of research in important crop species.…”
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Mutating alfalfa COUMARATE 3-HYDROXYLASE using multiplex CRISPR/Cas9 leads to reduced lignin deposition and improved forage quality by Tezera W. Wolabu, Kashif Mahmood, Fang Chen, Ivone Torres-Jerez, Michael Udvardi, Million Tadege, Lili Cong, Zengyu Wang, Jiangqi Wen

“…Four guide RNAs (gRNAs) targeting the first exon of MsC3H were designed and clustered into a tRNA-gRNA polycistronic system and introduced into tetraploid alfalfa via Agrobacterium-mediated transformation. Out of 130 transgenic lines, at least 73 lines were confirmed to contain gene-editing events in one or more alleles of MsC3H. …”
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Carbon Catabolite Repression Relaxation: Approaches for Sugar Co-Utilization in Escherichia coli by Fox, Kevin J.

“…The expression of udh from Pseudomonas syringae and gli from Agrobacterium fabrum, along with the inactivation of garK, encoding for glycerate kinase, enables D-glyceric acid accumulation by utilizing the endogenous expression of garD, garL, and garR. …”
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PERAN RHIZOBAKTERI DALAM FITOSTABILISASI LOGAM BERAT KROMIUM by , Ali Pramono, SP., , Dr. Ir. Irfan D. Prijambada, M.Eng.

“…Based on partial sequencing of 16S rRNA gene, isolate 3 was identified as Agrobacterium sp.…”

The molecular biology of chemotactic signal transduction in rhodobacter sphaeroides by Bell, A, Adam Warwick Bell

“…<em>Caulobacter</em>, <em>Agrobacterium</em>, <em>Rhizobium</em> and <em>Azospirillum</em> species (α-group proteobacteria) all show behavioural similarities, and there are genetic clues to suggest that these organisms possess a dual chemotactic sensory transduction similar to the one we have found in <em>R. sphaeroides</em>.…”

Genetically modified organism detection of insect and herbicide resistance traits by qualitative and quantitative plasmid-based methods by Khoo, Siew Ping

“…Important genetically modified traits include herbicide tolerance traits encoded by the 5’-enolpyruvylshikimate-3-phosphate synthase (Cp4 epsps) gene derived from Agrobacterium sp. and insect resistance traits encoded by the cry gene derived from Bacillus thuringiensis. …”
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Over-expression of the Pikh gene with a CaMV 35S promoter leads to improved blast disease (Magnaporthe oryzae) tolerance in rice by Azizi, Parisa, Yusop, Mohd Rafii, Abdullah, Siti Nor Akmar, Musa, Mohamed Hanafi, Mahmood, Maziah, Sahebi, Mahbod, Ashkani, Sadegh, Taheri, Sima, Jahromi, Mohammad Faseleh

“…Thus, a full DNA and coding DNA sequence (CDS) of the Pikh gene, 3172 bp, and 1206 bp in length, were obtained through amplifying the gDNA and cDNA template from a PH9-resistant rice variety using a specific primer. Agrobacterium-mediated transformation technology was also used to introduce the Pikh gene into the MR219 callus. …”
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The Anti-Biofouling Effect of Piper Betle Extract on Membrane Biofouling in Bioreactor for Batik Wastewater Treatment by Muhammad Faisal, Siddiqui

“…The autoinducers production in bioreactor was confirmed using an indicator strain Agrobacterium tumefaciens. Moreover, three different AHLs were found in biocake using thin layer chromatographic analysis. …”
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Function of <i>FT</i> in Flowering Induction in Two <i>Camellia</i> Species by Xiong Wang, Jiyuan Li, Hengfu Yin, Xinlei Li, Weixin Liu, Zhengqi Fan

“…Transgenic callus tissue was obtained by introducing the two genes into <i>C. azalea</i> through <i>Agrobacterium</i>-mediated transformation. Transcriptome and quantitative real-time polymerase chain reaction analyses indicated that both florigen <i>FT</i> genes promoted the expression of downstream genes such as <i>AP1</i>, <i>FUL</i>, and <i>SEP3</i>, and slightly up-regulated the expression of upstream genes such as <i>CO</i> and <i>GI</i>. …”
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