CRISPR/Cas9 Guided Mutagenesis of <i>Grain Size 3</i> Confers Increased Rice (<i>Oryza sativa</i> L.) Grain Length by Regulating Cysteine Proteinase Inhibitor and Ubiquitin-Related... by Babar Usman, Neng Zhao, Gul Nawaz, Baoxiang Qin, Fang Liu, Yaoguang Liu, Rongbai Li

“…Two target sites were designed to construct the vector, and the Agrobacterium-mediated method was used for rice transformation. …”
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Transcriptome Analysis of Fusarium–Tomato Interaction Based on an Updated Genome Annotation of <i>Fusarium oxysporum</i> f. sp. <i>lycopersici</i> Identifies Novel Effector Candida... by Xizhe Sun, Xiangling Fang, Dongmei Wang, David A. Jones, Lisong Ma

“…We examined the <i>Fol</i>-infection expression profiles of all 13 <i>SIX</i> genes present in <i>Fol</i> race 2 and identified 27 new candidate effector genes that were likewise significantly upregulated upon <i>Fol</i> infection. Using Agrobacterium-mediated transformation, we tested the ability of 22 of the new candidate effector genes to suppress or induce cell death in leaves of <i>Nicotiana benthamiana</i>. …”
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Simultaneous induction of mutant alleles of two allergenic genes in soybean by using site-directed mutagenesis by Shota Sugano, Aya Hirose, Yuhei Kanazashi, Kohei Adachi, Miki Hibara, Takeshi Itoh, Masafumi Mikami, Masaki Endo, Sakiko Hirose, Nobuyuki Maruyama, Jun Abe, Tetsuya Yamada

“…Results We used two guide RNAs (gRNAs) and Agrobacterium-mediated transformation for simultaneous site-directed mutagenesis of two genes encoding the major allergens Gly m Bd 28 K and Gly m Bd 30 K in two Japanese soybean varieties, Enrei and Kariyutaka. …”
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Identification of <i>miR397a</i> and Its Functional Characterization in Callus Growth and Development by Regulating Its Target in <i>Liriodendron</i> by Dan Wang, Fengjuan Lu, Ye Lu, Tielong Cheng, Jisen Shi, Jinhui Chen, Zhaodong Hao

“…Furthermore, to clarify the regulatory function of LhmiR397a-<i>LhLAC11</i>, transgenic calli overexpressing <i>LhMIR397a</i>, <i>LhLAC11</i>, and <i>mLhLAC11</i> were separately obtained by <i>Agrobacterium</i>-mediated transfer. The results showed that overexpressing <i>LhMIR397a</i> might retard callus proliferation, while overexpressing LhLAC11 or mLhLAC11 could promote callus proliferation. …”
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Advances in Genetics and Molecular Breeding of Broccoli by Fengqing Han, Yumei Liu, Zhiyuan Fang, Limei Yang, Mu Zhuang, Yangyong Zhang, Honghao Lv, Yong Wang, Jialei Ji, Zhansheng Li

“…Genetic engineering by the well-developed <i>Agrobacterium tumefaciens</i>-mediated transformation in broccoli has contributed to the improvement of quality; postharvest life; glucosinolate and sulforaphane content; and resistance to insects, pathogens and abiotic stresses. …”
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Functional Validation and Promoter DNA Methylation Analysis of the <i>OfPAO</i> Gene of <i>Osmanthus fragrans</i> ‘Yinbi Shuanghui’ by Rui Wang, Yixiao Zhou, Xuan Chen, Hao Wei, Dong Zheng, Wuwei Zhu, Lianggui Wang, Xiulian Yang

“…Then, we constructed an <i>OfPAO</i> overexpression vector, before transforming it into tobacco through an Agrobacterium-mediated transformation to obtain transgenic plants. …”
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Growth‐Coupled Evolutionary Pressure Improving Epimerases for D‐Allulose Biosynthesis Using a Biosensor‐Assisted In Vivo Selection Platform by Chao Li, Xin Gao, Huimin Li, Tong Wang, Fuping Lu, Hui‐Min Qin

“…Structural analysis and evolutionary conservation analysis of Agrobacterium sp. SUL3 D‐allulose 3‐epimerase (ADAE) reveal a highly conserved catalytic active site and variable hydrophobic pocket, which together regulate substrate recognition. …”
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The Nexus between Fire and Soil Bacterial Diversity in the African Miombo Woodlands of Niassa Special Reserve, Mozambique by Ivete Sandra Alberto Maquia, Paula Fareleira, Isabel Videira e. Castro, Ricardo Soares, Denise R. A. Brito, Aires Afonso Mbanze, Aniceto Chaúque, Cristina Máguas, Obinna T. Ezeokoli, Natasha Sofia Ribeiro, Isabel Marques, Ana I. Ribeiro-Barros

“…A diverse pool of diazotrophs was isolated, and included symbiotic (e.g., <i>Mesorhizobium</i> sp., <i>Neorhizobium galegae</i>, <i>Rhizobium</i> sp., and <i>Ensifer</i> adhaerens), and non-symbiotic (e.g., <i>Agrobacterium</i> sp., <i>Burkholderia</i> sp., <i>Cohnella</i> sp., <i>Microvirga</i> sp., <i>Pseudomonas</i> sp., and <i>Stenotrophomonas</i> sp.) bacteria. …”
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Genome editing in rice mediated by miniature size Cas nuclease SpCas12f by Satoru Sukegawa, Osamu Nureki, Seiichi Toki, Seiichi Toki, Seiichi Toki, Seiichi Toki, Hiroaki Saika

“…An expression vector encoding rice codon-optimized SpCas12f and sgRNA for OsTubulin as a target was introduced into rice calli by Agrobacterium-mediated transformation. Molecular analysis of SpCas12f-transformed calli showed that mutations were introduced successfully into the target region. …”
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A necessity of introduction of the system of registration of GMO is for Ukraine by B. I. Nazar, H. V. Kushnir, H. I. Boiko, S. D. Murska

“…The qualitative determination of GMOs is based on the discovery of genetically modified (GM) targeted regulatory sequences of the 35S Cauliflower Mosaic Virus (CaMV) promoter or FMV promoter and/or the NOS (T-NOS) T1 terminator of Agrobacterium tumefaciens plasmids that are introduced into the genetic constructs of GM based on the use of the multiplex polymerase chain reaction method to obtain real-time research results – PCR RF.…”
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Effects of AMF Compound Inoculants on Growth, Ion Homeostasis, and Salt Tolerance-Related Gene Expression in Oryza sativa L. Under Salt Treatments by Bo Zhang, Feng Shi, Xu Zheng, Hongyang Pan, Yuqiang Wen, Fuqiang Song

“…Therefore, the compound inoculum Funneliformis mosseae (Fm) together with two functional microorganisms, Piriformospora indica (Pi) and Agrobacterium rhizogenes (Ar) was evaluated for their effect on the rice growth, photosynthetic gas exchange parameters, ion homeostasis, and the expression of salt tolerance-related genes under 0, 80, 120 and 160 mM salt stress conditions. …”
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In-silico analysis and transformation of OsMYB48 transcription factor driven by CaMV35S promoter in model plant – Nicotiana tabacum L. conferring abiotic stress tolerance by Yumna Ahmad, Saqlain Haider, Javed Iqbal, Sana Naseer, Kotb A. Attia, Arif Ahmed Mohammed, Sajid Fiaz, Tariq Mahmood

“…The transformation of OsMYB TF into tobacco was carried out using the Agrobacterium-mediated transformation method, to further analyze the expression level of a gene in different plant parts, under stress conditions. …”
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Construction and Identification of Infectious Clone from Sri Lankan Cassava Mosaic Virus Tvm3 Isolate by Naitong YU, Shuli XIAN, Huixiang YIN, Yuhan ZHAO, Xiaobao ZHENG, Zhixin LIU

“…【Method】In this study, SLCMV TVM3 strain was used as the object, and its 1.10 mer DNA-A and 1.08 mer DNA-B components were respectively constructed into pCAMBIA1301 vector, and the viral infectious clone vectors pCAMBIA1301-DNA-A (pDNA-A) and pCAMBIA1301-DNA-B (pDNA-B) were obtained. Agrobacterium tumefacien GV3101 mediated pDNA-A and pDNA-B co-infected tobacco and Arabidopsis plants.…”
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<i>PhCHS5</i> and <i>PhF3′5′H</i> Genes Over-Expression in <i>Petunia</i> (<i>Petunia hybrida</i>) and <i>Phalaenopsis</i> (<i>Phalaenopsis aphrodite</i>) Regulate Flower Color and... by Yuxia Lou, Qiyu Zhang, Qingyu Xu, Xinyu Yu, Wenxin Wang, Ruonan Gai, Feng Ming

“…These genes were transformed into both <i>Petunia</i> and <i>Phalaenopsis</i> plants using a gene gun or an <i>Agrobacterium tumefaciens</i>-based method. Compared with WT, <i>35S::PhCHS5</i> and <i>35S::PhF3′5′H</i> both had deeper color and higher anthocyanin content in <i>Petunia</i> plants. …”
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In Vitro Studies of Endophytic Bacteria Isolated from Ginger (<i>Zingiber officinale</i>) as Potential Plant-Growth-Promoting and Biocontrol Agents against <i>Botrytis cinerea</i>... by Alejandro Bódalo, Rogelio Borrego, Carlos Garrido, Hernando José Bolivar-Anillo, Jesús M. Cantoral, María Dolores Vela-Delgado, Victoria E. González-Rodríguez, María Carbú

“…The endophytic bacteria genera <i>Lelliottia</i>, <i>Lysinibacillus</i>, <i>Kocuria</i>, <i>Agrococcus</i>, <i>Acinetobacter</i>, <i>Agrobacterium</i>, <i>Zymobacter</i>, and <i>Mycolicibacterium</i> were identified. …”
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Enhancing drought tolerance in cauliflower (Brassica oleracea var. botrytis) by targeting LFY transcription factor modulation via the ethylene precursor, ACCA: an innovative comput... by Sandip Debnath, Abdallah M. Elgorban, Ali H. Bahkali, Rajalakshmanan Eswaramoorthy, Meenakshi Verma, Asad Syed, Vetriselvan Subramaniyan, Chinnaperumal Kamaraj, Ling Shing Wong, Vinoth Kumarasamy

“…The dual role of beneficial and detrimental Agrobacterium was scrutinized, particularly emphasizing the ethylene precursor compound, 1-amino-cyclopropane-1-carboxylic acid (ACCA).ResultsACCA possessed salient PGP traits, particularly demonstrating a pronounced enhancement of drought resistance in cauliflower plants. …”
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PHÂN LẬP VÀ NHẬN DIỆN VI KHUẨN VÙNG RỄ KÍCH THÍCH SỰ SINH TRƯỞNG (PGPR) TỪ MỘT SỐ LOẠI RAU ĂN LÁ TRỒNG TẠI THÀNH PHỐ CẦN THƠ by Trần Thị Giang, Cao Ngọc Điệp, Nguyễn Thị Quyên

“…Kết quả cho thấy dòng NBT613 tương đồng 99% với dòng GQ181060 Agrobacterium tumefaciens BLN4, dòng NPD855 tương đồng 99% với dòng KC934864 Ensifer adhaerens M27 và JQ322555 Sinorhizobium meliloti CHW10B, dòng PBT622 tương đồng 97% với dòng KF358257 Acinetobacter calcoaceticus L14, dòng POM112 tương đồng 97% với dòng JQ923444 Achromobacter xylosoxidans BL6, dòng NBT625 tương đồng 98% với dòng KF870446 Rhizobium sp. …”
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Enhancing powdery mildew resistance in soybean by targeted mutation of MLO genes using the CRISPR/Cas9 system by Thao Phuong Bui, Huy Le, Dong Thi Ta, Cuong Xuan Nguyen, Ngoc Thu Le, Truong Thi Tran, Phuong Van Nguyen, Gary Stacey, Minviluz G. Stacey, Ngoc Bich Pham, Ha Hoang Chu, Phat Tien Do

“…Results A dual-sgRNA CRISPR/Cas9 construct was designed and successfully transferred into the Vietnamese soybean cultivar DT26 through Agrobacterium tumefaciens-mediated transformation. Various mutant forms of the GmMLO genes including biallelic, chimeric and homozygous were found at the T0 generation. …”
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Transgenic ZmMYB167 Miscanthus sinensis with increased lignin to boost bioenergy generation for the bioeconomy by Rakesh Bhatia, Emma Timms-Taravella, Luned A. Roberts, Odin M. Moron-Garcia, Barbara Hauck, Sue Dalton, Joe A. Gallagher, Moritz Wagner, John Clifton-Brown, Maurice Bosch

“…Results For this purpose, transgenic M. sinensis were generated by Agrobacterium-mediated transformation for expression of ZmMYB167, a MYB transcription factor known for regulating lignin biosynthesis in C3 and C4 grasses. …”
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A Cotyledon-based Virus-Induced Gene Silencing (Cotyledon-VIGS) approach to study specialized metabolism in medicinal plants by Yongliang Liu, Ruiqing Lyu, Joshua J. Singleton, Barunava Patra, Sitakanta Pattanaik, Ling Yuan

“…The seedlings were vacuum-infiltrated with Agrobacterium tumefaciens GV3101 cells carrying the tobacco rattle virus (TRV) vectors. …”
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