Overexpression of galactinol synthase 1 from Solanum commersonii (ScGolS1) confers freezing tolerance in transgenic potato by Feiyan He, Jianfei Xu, Yinqiao Jian, Shaoguang Duan, Jun Hu, Liping Jin, Guangcun Li

“…In this study, the ScGolS1 gene from Solanum commersonii was cloned and introduced into the S. tuberosum cultivars ‘Atlantic’ and ‘Desiree’ via Agrobacterium-mediated transformation. Phenotyping assays showed that overexpression of ScGolS1 could significantly improve freezing tolerance in transgenic potato plants. …”
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Heterologous Expression of the <i>Constitutive Disease Resistance 2</i> and <i>8</i> Genes from <i>Poncirus trifoliata</i> Restored the Hypersensitive Response and Resistance of <i... by Xiaobao Ying, Bryce Redfern, Frederick G. Gmitter, Zhanao Deng

“…Our results showed that <i>PtCDR2</i> and <i>PtCDR8</i> were functional in <i>Arabidopsis</i> and played a positive role in disease resistance and demonstrated that <i>Arabidopsis</i> mutants can be a useful alternate system for screening <i>Poncirus</i> genes before making the time-consuming effort to transfer them into citrus, a perennial woody plant that is highly recalcitrant for <i>Agrobacterium</i> or biolistic-mediated transformation.…”
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Identification and Functional Analysis of the <i>C</i><i>gNAC043</i> Gene Involved in Lignin Synthesis from <i>Citrus</i><i>grandis</i> “San Hong” by Xiaoting Li, Naiyu Wang, Wenqin She, Zhixiong Guo, Heli Pan, Yuan Yu, Jianwen Ye, Dongming Pan, Tengfei Pan

“…A transformation vector, p1301- <i>CgNAC043</i>, was transformed into the mesocarp of ‘San hong’ by <i>Agrobacterium</i>-mediated transformation. Results showed that the expression of transcription factors <i>CgMYB58</i> and <i>CgMYB46</i> are all upregulated. …”
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Enhanced Production of Nitrogenated Metabolites with Anticancer Potential in <i>Aristolochia manshuriensis</i> Hairy Root Cultures by Yury N. Shkryl, Galina K. Tchernoded, Yulia A. Yugay, Valeria P. Grigorchuk, Maria R. Sorokina, Tatiana Y. Gorpenchenko, Olesya D. Kudinova, Anton I. Degtyarenko, Maria S. Onishchenko, Nikita A. Shved, Vadim V. Kumeiko, Victor P. Bulgakov

“…Herein, we established hairy root cultures of <i>A. manshuriensis</i> transformed with <i>Agrobacterium rhizogenes</i> root oncogenic loci (<i>rol)B</i> and <i>rolC</i> genes. …”
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Function verification of a chlorophyll a/b binding protein gene through a newly established tobacco rattle virus-induced gene silencing system in Kandelia obovata by Mingxiong Zhang, Mingxiong Zhang, Yuhui Rao, Xiaofeng Chen, Yunrui Shi, Chonglong Wei, Xianfeng Wang, Lu Wang, Lu Wang, Chengjin Xie, Chengjin Xie, Chenglang Pan, Chenglang Pan, Jianming Chen, Jianming Chen

“…Besides, the system was also used to verify the role of a Chlorophyll a/b binding protein (Cab) gene KoCAB in leaf carbon sequestration of K. obovata.RNA-Seq and qRT-PCR showed that the highest gene-silencing efficiency could reach 90% after 10 days of inoculation and maintain above 80% after 15 days, which was achieved with resuspension buffer at pH 5.8 and Agrobacterium culture at OD600 of 0.4-0.6. Taken together, the TRV-mediated VIGS system established herein is the first genetic analysis tool for mangroves, which may greatly impel functional genomics studies in mangrove plants.…”
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Overexpression of the <i>Rubus idaeus</i> Polygalacturonases Gene <i>RiPG2</i> Accelerates Fruit Softening in <i>Solanum lycopersicum</i> by Tiemei Li, Xiao Guo, Yuxiao Chen, Jing Li, Caihong Yu, Zhifeng Guo, Guohui Yang

“…‘Polka’ fruits and tomato plants overexpressing <i>RiPG2</i> were obtained by <i>Agrobacterium tumefaciens</i>-mediated leaf disc transformation to elucidate the role of <i>RiPG2</i> in fruit softening. …”
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Isolation of Bioactive Compounds, Antibacterial Activity, and Action Mechanism of Spore Powder From Aspergillus niger xj by Longfeng Wei, Longfeng Wei, Qinyu Zhang, Ailin Xie, Yang Xiao, Kun Guo, Shuzhen Mu, Yudan Xie, Zhu Li, Zhu Li, Tengxia He

“…This study was conducted to investigate the bioactive compounds of Aspergillus niger xj, three plant pathogens (Agrobacterium tumefaciens T-37, Erwinia carotovora EC-1, and Ralstonia solanacearum RS-2) were used as indicator bacteria, according to the biological activity tracking, five compounds were isolated from A. niger xj spore powder, and characterization of compounds was done by NMR (1H-NMR and 13C-NMR) and EI-MS and was identified as ergosterol (1), β-sitosterol (2), 5-pentadecylresorcinol (3), 5-hydroxymethyl-2-furancarboxylic acid (4), and succinimide (5). …”
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Overexpression of the Golden SNP-Carrying <i>Orange</i> Gene Enhances Carotenoid Accumulation and Heat Stress Tolerance in Sweetpotato Plants by So-Eun Kim, Chan-Ju Lee, Sul-U Park, Ye-Hoon Lim, Woo Sung Park, Hye-Jin Kim, Mi-Jeong Ahn, Sang-Soo Kwak, Ho Soo Kim

“…In this study, we generated transgenic sweetpotato plants overexpressing <i>IbOr</i>-<i>R96H</i> under the control of the cauliflower mosaic virus (CaMV) <i>35S</i> promoter via <i>Agrobacterium</i>-mediated transformation. The total carotenoid contents of <i>IbOr</i>-<i>R96H</i> storage roots (light-orange flesh) and <i>IbOr</i>-<i>WT</i> storage roots (light-yellow flesh) were 5.4–19.6 and 3.2-fold higher, respectively, than those of non-transgenic (NT) storage roots (white flesh). …”
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Genome Editing of Rice <i>eIF4G</i> Loci Confers Partial Resistance to Rice Black-Streaked Dwarf Virus by Wei Wang, Shuhui Ma, Peng Hu, Yinghua Ji, Feng Sun

“…This recombinant vector was used to generate mutations in the rice cultivar Nipponbare via <i>Agrobacterium</i>-mediated transformation. This approach produced heritable homozygous mutations in the transgene-free T1 generation. …”
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Heterologous Expression of <i>Platycodon grandiflorus PgF3′5′H</i> Modifies Flower Color Pigmentation in Tobacco by Lulin Ma, Wenjie Jia, Qing Duan, Wenwen Du, Xiang Li, Guangfen Cui, Xiangning Wang, Jihua Wang

“…In this study, <i>PgF3′5′H</i> was subcloned into a plant over-expression vector and transformed into tobacco via <i>Agrobacterium tumefaciens</i> to investigate its catalytic function. …”
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The <i>MdAux/IAA2</i> Transcription Repressor Regulates Cell and Fruit Size in Apple Fruit by Haidong Bu, Xiaohuan Sun, Pengtao Yue, Junling Qiao, Jiamao Sun, Aide Wang, Hui Yuan, Wenquan Yu

“…In this study, we identified the function of the <i>MdAux/IAA2</i> gene in apple fruit size difference using <i>Agrobacterium</i>-mediated genetic transformation. Overexpression of <i>MdAux/IAA2</i> decreased the apple flesh callus increment and caused a smaller globular cell size. …”
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Knockdown of GmD53a confers strigolactones mediated rhizobia interaction and promotes nodulation in soybean by Naveed Rehman, Fahim Ullah Khan, Muhammad Imran, Shahid Ali Rajput, Yiming Li, Ihteram Ullah, Rana waseem Akhtar, Muhammad Imran, Arwa Abdulkreem AL-Huqail, Ahmad El Askary, Amany Salah Khalifa, Muhammad Tehseen Azhar

“…To induce transgenic hairy roots of soybean, electric shock was used to transform pB7WG1WG2 vectors containing GmD53a knockdown and GUS into K599 strains of Agrobacterium rhizogenes. The hairy roots and nodules were collected and examined for root nodules ratio and RNA was extracted after 4 weeks of rhizobia inoculation. …”
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<i>Paracoccidioides lutzii</i> Formamidase Contributes to Fungal Survival in Macrophages by Lana O’Hara Souza Silva, Thalison Rodrigues Moreira, Relber Aguiar Gonçales, Mariana Vieira Tomazett, Juliana Alves Parente-Rocha, Karine Mattos, Juliano Domiraci Paccez, Orville Hernandez Ruiz, Maristela Pereira, Célia Maria de Almeida Soares, Simone Schneider Weber, Vanessa Rafaela Milhomem Cruz-Leite, Clayton Luiz Borges

“…Thereby, we developed a <i>P. lutzii</i> silenced strain of <i>fmd</i> gene (AsFmd) by antisense RNA technology using <i>Agrobacterium tumefaciens</i>-mediated transformation (ATMT). …”
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Methyl jasmonate enhances ursolic, oleanolic and rosmarinic acid production and sucrose induced biomass accumulation, in hairy roots of Lepechinia caulescens by Victor M. Vergara-Martínez, Samuel E. Estrada-Soto, Susana Valencia-Díaz, Karlina Garcia-Sosa, Luis Manuel Peña-Rodríguez, José de Jesús Arellano-García, Irene Perea-Arango

“…Methods Leaves from plants of L. caulescens were inoculated with Agrobacterium rhizogenes strain ATCC 15834. PCR of rolB gene confirmed the transgenic nature of hairy roots. …”
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FungalBraid 2.0: expanding the synthetic biology toolbox for the biotechnological exploitation of filamentous fungi by Elena Moreno-Giménez, Elena Moreno-Giménez, Mónica Gandía, Zara Sáez, Paloma Manzanares, Lynne Yenush, Diego Orzáez, Jose F. Marcos, Sandra Garrigues

“…The FungalBraid (FB) modular cloning platform enables interchangeability of DNA parts with the GoldenBraid (GB) platform, which is designed for plants, and other systems that are compatible with the standard Golden Gate cloning and syntax, and uses binary pCAMBIA-derived vectors to allow Agrobacterium tumefaciens-mediated transformation of a wide range of fungal species. …”
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Optimization of highly efficient exogenous-DNA-free Cas9-ribonucleoprotein mediated gene editing in disease susceptibility loci in wheat (Triticum aestivum L.) by Snigdha Poddar, Snigdha Poddar, Jaclyn Tanaka, Katherine L. D. Running, Gayan K. Kariyawasam, Justin D. Faris, Timothy L. Friesen, Myeong-Je Cho, Jamie H. D. Cate, Jamie H. D. Cate, Jamie H. D. Cate, Brian Staskawicz, Brian Staskawicz

“…To this end, targeted double-stranded break technology using RNA-guided Cas9 has been adopted widely for genome editing in plants. Agrobacterium or particle bombardment-based delivery of plasmids encoding Cas9 and guide RNA (gRNA) is common, but requires optimization of expression and often results in random integration of plasmid DNA into the plant genome. …”
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Engineered Fungus <i>Thermothelomyces thermophilus</i> Producing Plant Storage Proteins by Larissa Balabanova, Aleksandra Seitkalieva, Yulia Yugay, Tatiana Rusapetova, Lubov Slepchenko, Anna Podvolotskaya, Margarita Yatsunskaya, Elena Vasyutkina, Oksana Son, Liudmila Tekutyeva, Yury Shkryl

“…An efficient <i>Agrobacterium</i>-mediated genetic transformation based on the plant binary vector pPZP-RCS2 was carried out for the multiple heterologous protein production in filamentous fungus <i>Thermothelomyces</i> <i>thermophilus</i> F-859 (formerly <i>Myceliophthora</i> <i>thermophila </i>F-859). …”
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Two genes encoded by mulberry crinkle leaf virus (MCLV): The V4 gene enhances viral replication, and the V5 gene is needed for MCLV infection in Nicotiana benthamiana by Tao-Tao Han, Jia-Xuan Tang, Miao Fang, Peng Zhang, Pei-Yu Han, Zhen-Ni Yin, Yu Ma, Jian Zhang, Quan-You Lu

“…The functions of V4 and V5 were investigated by constructing Agrobacterium-mediated infectious clones of wild-type MCLV variant П (MCLV vII), MCLVwt and MCLV vП mutants, such as MCLVmV4 (start codon of V4 ORF mutated), MCLVdV4 (5′-end partial deletion of V4 ORF sequence) and MCLVmV5 (V5 ORF start codon mutated). …”
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The potential of PGPR and Trichoderma-based bioproducts and resistant cultivars as tools to manage clubroot disease in cruciferous crops by Carlos Andrés Moreno-Velandia, Francy Liliana Garcia-Arias, Lorena Dávila-Mora, Edwin Rodríguez, Alejandro Villabona-Gélvez, Alejandro Villabona-Gélvez, Eliana Gisela Revelo-Gómez, Carlos Alberto Marcillo-Paguay, Donald Heberth Riascos-Ortiz, Andrea Paola Zuluaga

“…Two commercial bioproducts were evaluated in susceptible broccoli, one based on the PGPR consortium (Bacillus amyloliquefaciens, Bacillus pumilus, and Agrobacterium radiobacter K84) and the other one based on Trichoderma koningiopsis Th003 (Tricotec® WG). …”
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Harnessing Novel Soil Bacteria for Beneficial Interactions with Soybean by Osiel Silva Gonçalves, Thamires Santos Souza, Guilherme de Castro Gonçalves, Alexia Suellen Fernandes, Tomás Gomes Reis Veloso, Sumaya Martins Tupy, Ediones Amaro Garcia, Mateus Ferreira Santana

“…We also reported the draft genome sequences of <i>Kosakonia</i> sp. strain SOY2 and <i>Agrobacterium</i> sp. strain SOY23. Along with our analysis of 169 publicly available genomes for the genera reported here, we demonstrated that these bacteria have a repertoire of genes encoding plant growth-promoting proteins and secondary metabolite biosynthetic gene clusters that directly affect plant growth. …”
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