Candidate pathogenicity factor/effector proteins of ‘Candidatus Phytoplasma solani’ modulate plant carbohydrate metabolism, accelerate the ascorbate–glutathione cycle, and induce a... by Marina Dermastia, Špela Tomaž, Špela Tomaž, Rebeka Strah, Rebeka Strah, Tjaša Lukan, Anna Coll, Barbara Dušak, Barbara Dušak, Barbara Anžič, Timotej Čepin, Stefanie Wienkoop, Aleš Kladnik, Maja Zagorščak, Monika Riedle-Bauer, Christina Schönhuber, Wolfram Weckwerth, Wolfram Weckwerth, Kristina Gruden, Thomas Roitsch, Maruša Pompe Novak, Maruša Pompe Novak, Günter Brader

“…The way in which they manipulate the host cellular machinery was elucidated by analyzing Nicotiana benthamiana leaves after Agrobacterium-mediated transient transformation with the pathogenicity factor/effector constructs using confocal microscopy, pull-down, and co-immunoprecipitation, and enzyme assays. …”
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Development of a High Oleic Cardoon Cell Culture Platform by SAD Overexpression and RNAi-Mediated FAD2.2 Silencing by Elisa Cappetta, Monica De Palma, Rosa D’Alessandro, Alessandra Aiello, Raffaele Romano, Giulia Graziani, Alberto Ritieni, Dario Paolo, Franca Locatelli, Francesca Sparvoli, Teresa Docimo, Marina Tucci

“…With the aim of boosting their potential uses, we designed a biotechnological approach to increase oleic acid content through Agrobacterium tumefaciens-mediated metabolic engineering. …”
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Investigation of <i>PtSGT1</i> and <i>PtSGT4</i> Function in Cellulose Biosynthesis in <i>Populus tomentosa</i> Using CRISPR/Cas9 Technology by Yinxuan Xue, Siyan Li, Deyu Miao, Sai Huang, Bin Guo, Shanwen Li, Xin-Min An

“…In the present study, CRISPR/Cas9 gene-editing technology was used to construct <i>PtSGT1-</i>sgRNA and <i>PtSGT4-</i>sgRNA expression vectors, which were genetically transformed into <i>P. tomentosa</i> using the <i>Agrobacterium</i>-mediated method to obtain transgenic lines. …”
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Acidovorax citrulli type III effector AopU interferes with plant immune responses and interacts with a watermelon E3 ubiquitin ligase by Linlin Yang, Linlin Yang, Mei Zhao, Xiaoxiao Zhang, Jie Jiang, Nuoya Fei, Nuoya Fei, Weiqin Ji, Yunfeng Ye, Wei Guan, Yuwen Yang, Yuwen Yang, Tingchang Zhao, Tingchang Zhao

“…In this study, we characterized a novel T3E called AopU in A. citrulli group II strain Aac5, which shares homology with XopU in Xanthomonas oryzae. The Agrobacterium-mediated gene transient expression system was used to study the effect of AopU on host immunity. …”
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Synthesis of Functional Silver Nanoparticles and Microparticles with Modifiers and Evaluation of Their Antimicrobial, Anticancer, and Antioxidant Activity by Erum Dilshad, Mehmoona Bibi, Nadeem Ahmed Sheikh, Khairul Fikri Tamrin, Qaisar Mansoor, Qaisar Maqbool, Muhammad Nawaz

“…Functionally, capped silver particles exhibited antibacterial activity against Gram-positive (<i>Staphylococcus aureus, Bacillus subtilis,</i> and <i>Micrococcus luteus</i>) and Gram-negative bacteria (<i>Salmonella setubal, Enterobacter aerogenes,</i> and <i>Agrobacterium tumefaciens</i>). The bactericidal activity was more active against Gram-negative bacteria with minimum inhibitory concentration (MIC) as low as 5 ppm as compared to 25 ppm for Gram-positive. …”
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Elevation of artemisinin content by co-transformation of artemisinin biosynthetic pathway genes and trichome-specific transcription factors in Artemisia annua by Danial Hassani, Danial Hassani, Ayat Taheri, Xueqing Fu, Wei Qin, Liu Hang, Yanan Ma, Kexuan Tang

“…The simultaneous co-transformation of these vectors by Agrobacterium resulted in the successful increase of the artemisinin content in T0 transgenic lines by up to 3.2-fold (2.72%) leaf dry weight compared to the control plants. …”
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Construction of Rice Mutants of the Polyamine Oxidase Gene OsPAO4 Based on CRISPR/Cas9 Editing by Chunzi LIN, Qiwei HUANG, Yan HOU, Shang GAO, Zhikai HAN, Danhong WEI, Chun CHEN, Jiafeng WANG

“…Then, the rice Pik-H4 NIL callus was transformed by Agrobacterium-mediated method. Positive transgenic plants were obtained by regeneration culture and hygromycin detection, PCR and sequence analysis were performed on the DNA sequences near the target site in the T0 generation plants.…”
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Secreted Glycosyltransferase RsIA_GT of <i>Rhizoctonia solani</i> AG-1 IA Inhibits Defense Responses in <i>Nicotiana benthamiana</i> by Danhua Zhang, Zhaoyilin Wang, Naoki Yamamoto, Mingyue Wang, Xiaoqun Yi, Ping Li, Runmao Lin, Zohreh Nasimi, Kazunori Okada, Keiichi Mochida, Yoshiteru Noutoshi, Aiping Zheng

“…The SP-truncated form of RsIA_GT (RsIA_GT(ΔS)) expressed in <i>Escherichia coli-</i>induced lesion-like phenotype in rice leaves when applied to punched leaves. However, Agrobacterium-mediated transient expressions of both the full-length RsIA_GT and RsIA_GT(ΔS) did not induce cell death in <i>N. benthamiana</i> leaves. …”
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Transcriptome profiling reveals histone deacetylase 1 gene overexpression improves flavonoid, isoflavonoid, and phenylpropanoid metabolism in Arachis hypogaea hairy roots by Liangchen Su, Shuai Liu, Xing Liu, Baihong Zhang, Meijuan Li, Lidan Zeng, Ling Li

“…We analyzed the transformation of A. hypogaea hairy roots using Agrobacterium rhizogenes and RNA sequencing to identify differentially expressed genes that were assigned to specific metabolic pathways. …”
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Optimization of a Virus-Induced Gene Silencing System with Soybean yellow common mosaic virus for Gene Function Studies in Soybeans by Kil Hyun Kim, Seungmo Lim, Yang Jae Kang, Min Young Yoon, Moon Nam, Tae Hwan Jun, Min-Jung Seo, Seong-Bum Baek, Jeom-Ho Lee, Jung-Kyung Moon, Suk-Ha Lee, Su-Heon Lee, Hyoun-Sub Lim, Jae Sun Moon, Chang-Hwan Park

“…Following SYCMV: Glycine max-phytoene desaturase (GmPDS) infiltration, we investigated the effect of photoperiod, inoculation time, concentration of Agrobacterium inoculm, and growth temperature on VIGS efficiency. …”
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Production of marker-free transgenic plants from mature tissues of navel orange using a Cre/loxP site-recombination system by Yongrui He, Lanzhen Xu, Aihong Peng, Tiangang Lei, Qiang Li, Lixiao Yao, Guojin Jiang, Shanchun Chen, Zhengguo Li, Xiuping Zou

“…Here, we constructed an Agrobacterium tumefaciens-mediated transformation for generation of marker-free transgenic plants from navel orange (Citrus sinensis Osbeck) mature stems using a Cre-loxP recombination system. …”
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Using Multiplexed CRISPR/Cas9 for Suppression of Cotton Leaf Curl Virus by Barkha Binyameen, Zulqurnain Khan, Sultan Habibullah Khan, Aftab Ahmad, Nayla Munawar, Muhammad Salman Mubarik, Hasan Riaz, Zulfiqar Ali, Asif Ali Khan, Alaa T. Qusmani, Kamel A. Abd-Elsalam, Sameer H. Qari

“…Confirmed constructs were moved into Agrobacterium and subsequently used for transformation. …”
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Effects of <i>Bt-Cry1Ah1</i> Transgenic Poplar on Target and Non-Target Pests and Their Parasitic Natural Enemy in Field and Laboratory Trials by Pu Wang, Hui Wei, Weibo Sun, Lingling Li, Peijun Zhou, Dawei Li, Zhuge Qiang

“…We generated a <i>Bt-Cry1Ah1</i> gene incorporating codon optimization and transferred it into <i>Populus deltoides</i> × <i>P. euramericana</i> cv “Nanlin895” using an Agrobacterium-mediated method. The resulting <i>Bt-Cry1Ah1</i> transgenic poplars were planted in the field with permission from the State Forestry Administration in 2017. …”
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Rice genetic engineering using transformation of Deeper Rooting1 and Phosphorus-Starvation Tolerance1 genes by Mehrbanoo Kazemi, Zahra Ghorbanzadeh, Leila Pourhang, Seyyed Mohammad Mousavi pakzad, Elahe Moatamed, Mona Mapar, Aliakbar Ebadi, Mohammad Reza Ghaffari, Ghasem Hosseini Salekdeh, Behzad Ghareyazie, Motahhareh Mohsenpour

“…The resulting construct, pUhrDroPstol is transformed into the Agrobacterium tumefactions strain EHA105 and used for the gene transformation into Hashemi cultivar. …”
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PENINGKATAN SERAPAN KROMIUM OLEH TANAMAN YANG DIINOKULASI RHIZOBAKTERI by , MMA. Retno Rosariastuti, Ir.,M.Si., , Prof. Ir. Irfan D. Prijambada, M.Eng., Ph.D.

“…The result of the identification, Isolate I3was identified as genus Rhizobium sp., and isolates I13 , I20 , I26, I30, I37, and I38 were identified as genus Agrobacterium sp. Based on the bioassay on jute, Isolate I3 and combination of isolate I3+compost in grumusol and regosol, efective in increasing plant dry weight and root + shoot chromium uptake (phytostabilization and phytoextraction) 2 times. …”

Functional Analysis of Oil Palm Metallothionein-Like Gene Promoter (MT3-A) in Bacteria and Transgenic Tomato by Kamaladini, Hossein

“…The application of different Zn2+ concentrations resulted in a strong decrease in the GUS activity and GUS mRNA level in E. coli and A. tumefaciens. The results of Agrobacterium-mediated transformation of tomato showed that MS medium containing 2 mg/L zeatin, 6 mg/L hygromycin and 300 mg/L of timentin was optimum to acquire the maximum putative shoot regeneration of tomato var. …”

Development Of In Vitro Regeneration System For Capsicum Annuum And Transformation With Cucumber Mosaic Virus Coat Protein Gene by Pua, Teen Lee

“…The minimal inhibitory concentrations of kanamycin were 100 and 200 mg/l(w/v) for cotyledonary leaves and hypocotyls, respectively. Agrobacterium tumefaciens strain EHA105 harbouring the CMV CP construct was used to transform the chilli hypocotyls and cotyledonary leaf explants. …”
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A Multiplex PCR System for the Screening of Genetically Modified (GM) Maize and the Detection of 29 GM Maize Events Based on Capillary Electrophoresis by Hongmei Yi, Ziyue Liang, Jianrong Ge, Haibo Zhang, Fengze Liu, Xuezhen Ren, Jie Ren, Haijie Wang, Jiali Ren, Xingxu Ren, Ying Zhang, Fang Jin, Shiqiao Jin, Yikun Zhao, Fengge Wang

“…To screen for GM maize events, we developed a multiplex PCR system to specifically detect 29 GM maize events as well as the cauliflower mosaic virus 35S promoter, the <i>Agrobacterium tumefaciens nos</i> terminator, the <i>Streptomyces viridochromogenes pat</i> gene, and the endogenous <i>zSSIIb</i> maize reference gene. …”
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Genome-wide characterization of L-aspartate oxidase genes in wheat and their potential roles in the responses to wheat disease and abiotic stresses by Yanqun Feng, Mingshuang Tang, Junhui Xiang, Pingu Liu, Youning Wang, Wang Chen, Zhengwu Fang, Wenli Wang

“…Meanwhile, the subcellular localization analysis of transient expression mediated by Agrobacterium tumetioniens indicated that TaAO3-6D was localized to chloroplasts. …”
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Ectopic Expression of <i>CsSUN</i> in Tomato Results in Elongated Fruit Shape via Regulation of Longitudinal Cell Division by Hao Li, Jing Han, Linjie Chen, Ni Han, Yajing Hu, Qian Ge, Zhonghai Ren, Lina Wang

“…Here, we introduced <i>CsSUN</i> into the round-fruited tomato inbred line ‘SN1′ (wild type, WT) via the <i>Agrobacterium tumefaciens</i>-mediated method. The high and constitutive expression of <i>CsSUN</i> was revealed by real-time PCR in all the tested tissues of the transgenic plants, especially in the fruits and ovaries. …”
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