CRISPR/Cas9 mutagenesis of the Arabidopsis GROWTH-REGULATING FACTOR (GRF) gene family by Juan Angulo, Christopher P. Astin, Olivia Bauer, Kelan J. Blash, Natalee M. Bowen, Nneoma J. Chukwudinma, Austin S. DiNofrio, Donald O. Faletti, Alexa M. Ghulam, Chloe M. Gusinde-Duffy, Kamaria J. Horace, Andrew M. Ingram, Kylie E. Isaack, Geon Jeong, Randolph J. Kiser, Jason S. Kobylanski, Madeline R. Long, Grace A. Manning, Julie M. Morales, Kevin H. Nguyen, Robin T. Pham, Monthip H. Phillips, Tanner W. Reel, Jenny E. Seo, Hiep D. Vo, Alexander M. Wukoson, Kathryn A. Yeary, Grace Y. Zheng, Wolfgang Lukowitz

“…Genome editing in plants typically relies on T-DNA plasmids that are mobilized by Agrobacterium-mediated transformation to deliver the CRISPR/Cas machinery. …”
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Biosynthesis of Functional Silver Nanoparticles Using Callus and Hairy Root Cultures of <i>Aristolochia manshuriensis</i> by Yulia A. Yugay, Maria R. Sorokina, Valeria P. Grigorchuk, Tatiana V. Rusapetova, Vladimir E. Silant’ev, Anna E. Egorova, Peter A. Adedibu, Olesya D. Kudinova, Elena A. Vasyutkina, Vladimir V. Ivanov, Alexander A. Karabtsov, Dmitriy V. Mashtalyar, Anton I. Degtyarenko, Olga V. Grishchenko, Vadim V. Kumeiko, Victor P. Bulgakov, Yury N. Shkryl

“…Furthermore, the findings unveiled the efficacy of the synthesized AgNPs against pathogenic microorganisms impacting both plants and animals, including <i>Agrobacterium rhizogenes</i>, <i>A. tumefaciens</i>, <i>Bacillus subtilis</i>, and <i>Escherichia coli</i>. …”
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Respiratory immune status and microbiome in recovered COVID-19 patients revealed by metatranscriptomic analyses by Huan Meng, Shuang Wang, Xiaomeng Tang, Jingjing Guo, Xinming Xu, Dagang Wang, Fangfang Jin, Mei Zheng, Shangqi Yin, Chaonan He, Ying Han, Jin Chen, Jinyu Han, Chaobo Ren, Yantao Gao, Huifang Liu, Yajie Wang, Ronghua Jin

“…Moreover, the random forest prediction model with differential genes showed a mean area under the curve (AUC) of 0.77, and KCNK12, IL17RD, LOC100507412, PTPRT, MYO15A, MPDZ, FLRT2, SPEG, SERPINB3, and KNDC1 were identified as the most important genes distinguishing the infected group from the recovered group. Agrobacterium tumefaciens, Klebsiella michiganensis, Acinetobacter pittii, Bacillus sp. …”
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Establishment of hairy root culture and its genetic transformation of Stephania tetrandra S. Moore for production of BIAs by Xiuhua Zhang, Junling Bu, Yujun Zhao, Qishuang Li, Xinyi Li, Ying Ma, Jinfu Tang, Jian Wang, Changjiangsheng Lai, Guanghong Cui, Juan Guo, Luqi Huang

“…Here, we constructed Agrobacterium rhizogenes (C58C1)-mediated hairy root culture and a co-transformation system in S. tetrandra to obtain a new source of bisbenzylisoquinoline alkaloids production. …”
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Development and evaluation of triple gene transgenic cotton lines expressing three genes (Cry1Ac-Cry2Ab-EPSPS) for lepidopteran insect pests and herbicide tolerance by Hamid Anees Siddiqui, Shaheen Asad, Rubab Zahra Naqvi, Muhammad Asif, Chengcheng Liu, Xin Liu, Muhammad Farooq, Saifullah Abro, Muhammad Rizwan, Muhammad Arshad, Muhammad Sarwar, Imran Amin, Zahid Mukhtar, Shahid Mansoor

“…Here, we have developed triple gene cotton containing EPSPS gene along with two Bt toxin genes Cry1Ac and Cry2Ab using a strategy where all three genes are cloned in the same T-DNA, followed by successful cotton transformation via Agrobacterium-mediated transformation. This strategy has been developed to help cotton breeders in developing new cultivars by incorporating these genes into the non-transgenic or single Bt (Cry1Ac) gene cotton background where all three genes will inherit together. …”
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Efficient control of the fungal pathogens Colletotrichum gloeosporioides and Penicillium digitatum infecting citrus fruits by native soilborne Bacillus velezensis strains by Tao Xuan Vu, Tram Bao Tran, Minh Binh Tran, Trang Thi Kim Do, Linh Mai Do, Mui Thi Dinh, Hanh-Dung Thai, Duc-Ngoc Pham, Van-Tuan Tran

“…By the heterologous expression of the green fluorescent protein (GFP) in C. gloeosporioides using Agrobacterium tumefaciens-mediated transformation, we could observe the fungal infection process of the citrus fruit stem-end rot caused by C. gloeosporioides for the first time. …”
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CRISPR/Cas9-Based Knock-Out of the <i>PMR4</i> Gene Reduces Susceptibility to Late Blight in Two Tomato Cultivars by Ruiling Li, Alex Maioli, Zhe Yan, Yuling Bai, Danila Valentino, Anna Maria Milani, Valerio Pompili, Cinzia Comino, Sergio Lanteri, Andrea Moglia, Alberto Acquadro

“…To verify the same effect in tomato in the present study, a CRISPR–Cas9 vector containing four single guide RNAs (sgRNAs: sgRNA1, sgRNA6, sgRNA7, and sgRNA8), targeting as many <i>SlPMR4</i> regions, was introduced via <i>Agrobacterium</i>-<i>tumefaciens</i>-mediated transformation into two widely grown Italian tomato cultivars: ‘San Marzano’ (SM) and ‘Oxheart’ (OX). …”
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OVEREXPRESSION OF WUSCHEL IMPROVES THE INDUCTION OF EMBRYOGENIC CALLUS IN SCALPS OF MUSA ACUMINATA L. AAA, CV. “GRAND NAIN” by Ana Ly Arroyo Herrera, Angela Kú González, Rosa María Escobedo Gracia-Medrano, Suemy Terezita Echeverria Echeverria, Miguel Angel Herrera Alamillo, Luis Joel Figueroa Yañez, Enrique Castaño, Luis Carlos Rodríguez Zapata

“…“Grand Nain” was carried out using a heterologous gene WUSCHEL from Arabidopsis thaliana, via vacuum infiltration with Agrobacterium tumefaciens, to establish and ascertain if its expression modifies the progression of the explants to the embryogenesis process and or reduces the time needed for in vitro embryogenic induction phase. …”
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Transcriptome analysis of Lr19-virulent mutants provides clues for the AvrLr19 of Puccinia triticina by Zhongchi Cui, Wenyue Wu, Fan Fan, Fei Wang, Daqun Liu, Dianping Di, Haiyan Wang

“…Screening AvrLr19 candidates by quantitative reverse transcription PCR (qPCR) and Agrobacterium-mediated transient assays in Nicotiana benthamiana.Results560 genes with single nucleotide polymorphisms (SNPs) and insertions or deletions (Indels) from non-differentially expressed genes were identified. …”
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Genome-Wide Characterization of the Aquaporin Gene Family in Radish and Functional Analysis of RsPIP2-6 Involved in Salt Stress by Xiaofang Yi, Xiaochuan Sun, Rong Tian, Kexin Li, Meng Ni, Jiali Ying, Liang Xu, Liwang Liu, Yan Wang

“…Additionally, overexpression of RsPIP2-6 could enhance salt tolerance by Agrobacterium rhizogenes-mediated transgenic radish hairy roots, which exhibited the mitigatory effects of plant growth reduction, leaf relative water content (RWC) reduction and alleviation of O2– in cells, as shown by nitro blue tetrazolium (NBT) staining, under salt stress. …”
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بیان پروانسولین انسانی در گیاه با استفاده از ناقل pVUT (Plasmid Viral University of Tehran) by Mahnaz Kheirollahi, Ali Akbar Shahnejat-Bushehri, Majid Kheirollahi, Fariba Abooei-Mehrizi, Hooshang Alizadeh

“…این دو سازه، به واسطه‌ی باکتری Agrobacterium tumefaciens pv. C58، به صورت بیان گذرا به گیاهان کاهو و یونجه انتقال داده شدند. …”
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Spontaneous Regeneration of Plantlets Derived from Hairy Root Cultures of <i>Lopezia racemosa</i> and the Cytotoxic Activity of Their Organic Extracts by Norely Vargas-Morales, Norma Elizabeth Moreno-Anzúrez, Janeth Téllez-Román, Irene Perea-Arango, Susana Valencia-Díaz, Alfonso Leija-Salas, Edgar R. Díaz-García, Pilar Nicasio-Torres, María Del Carmen Gutiérrez-Villafuerte, Jaime Tortoriello-García, Jesús Arellano-García

“…A histological analysis was performed with the aim of elucidating the spontaneous regeneration process of the hairy root lines LRT 2.3 and LRT 6.4, derived from <i>Lopezia racemosa</i> leaf explants and genetically transformed with the<i> Agrobacterium</i> <i>rhizogenes</i> strain ATCC15834/pTDT. …”
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Cellulase hyper-production by Trichoderma reesei mutant SEU-7 on lactose by Chengcheng Li, Fengming Lin, Le Zhou, Lei Qin, Bingzhi Li, Zhihua Zhou, Mingjie Jin, Zhan Chen

“…Results Trichoderma reesei mutant SEU-7 was constructed from T. reesei RUT-C30 with the overexpression of endogenous gene β-glucosidase (BGL1) by insertional mutagenesis via Agrobacterium tumefaciens-mediated transformation (AMT). …”
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The host niches of soybean rather than genetic modification or glyphosate application drive the assembly of root‐associated microbial communities by Minkai Yang, Fuhe Luo, Yuchen Song, Shenglin Ma, Yudi Ma, Aliya Fazal, Tongming Yin, Guihua Lu, Shucun Sun, Jinliang Qi, Zhongling Wen, Yongchun Li, Yonghua Yang

“…Moreover, two bacterial genera, Bradyrhizobium and Ensifer were more abundant in RT than in RS and SS, as well as three species, Agrobacterium radiobacter, Ensifer fredii and Ensifer meliloti, which are closely related to nitrogen‐fixation. …”
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Interacting with Hemoglobin: <i>Paracoccidioides</i> spp. Recruits hsp30 on Its Cell Surface for Enhanced Ability to Use This Iron Source by Aparecido Ferreira de Souza, Mariana Vieira Tomazett, Kleber Santiago Freitas e Silva, Juliana Santana de Curcio, Christie Ataides Pereira, Lilian Cristiane Baeza, Juliano Domiraci Paccez, Relber Aguiar Gonçales, Fernando Rodrigues, Maristela Pereira, Célia Maria de Almeida Soares

“…In order to investigate the importance of HSP30 in the <i>Paracoccidioides</i> genus, we developed a <i>Paracoccidioides brasiliensis</i> knockdown strain of HSP30 via <i>Agrobacterium tumefaciens</i>-mediated transformation and demonstrated that silencing this gene decreases the ability of <i>P. brasiliensis</i> to use hemoglobin as a nutrient source. …”
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Development of pineapple transgenic lines for the functional profiling of MIR535 gene family through the use of artificial microrna technology by Noor Hydayaty Md. Yusuf

“…Transgenes were then inserted into pineapple callus (MD2 hybrid) through Agrobacterium mediated transformation. Transgenic lines developed were used for expression profiling of amiRNAs and miR535's through stem-loop RT-qPCR. …”
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Vitamin E production in Amaranthus sp. and Allium porrum by co-suppression of homogentisate phytyltrransferase and tocopherol cyclase genes from Elaeis guineensis by Munusamy, Umaiyal

“…Thus, Amaranthus sp. and Allium porrum which do not accumulate α-tocotrienol naturally had shown to produce α-tocotrienol after suppression in the α-tocopherol production through transgenic manipulation. Finally, Agrobacterium -mediated transformation of inflorescence of Amaranthus sp. was carried out through drop by drop technique for producing transformed seeds. …”
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Establishment of tissue culture and transformation protocol for tropical corn (Zea mays L.) inbred lines by Matazu, Nasiruddeen Umar

“…Callus transformation was achieved using Agrobacterium tumefaciens strains LBA 4404 harboring pCAMBAIA1304 vector containing (mgfp5-gusA-His6 fusion). …”
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Root cultures and elicitation of secondary metabolites from Labisia pumila Benth & Hook. f. var. alata root by Tan, Sin Li

“…In this study, hairy roots of L. pumila were successfully established from leaf with petiole explants using three strains of Agrobacterium rhizogenes (A4, NCPPB2629 and NCPPB1855) which produced 16.67%, 70% and 40% transformation efficiencies, respectively.Strain A4 showed a rapid growth rate compared to strain NCPPB2629 and NCPPB1855 with a doubling time of 14 days. …”
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Identification and molecular characterisation of vascular-related defense genes against papaya dieback disease by Hamid, Muhammad Hanam

“…These genes were then sub cloned, in a sense orientation, into the pEAQ virus vector for subsequent transformation into papaya seedlings via Agrobacterium tumefaciens strain GV3101. The constructs were designated as pEAQ.NHO1, pEAQ.PR1, pEAQ.EFR, pEAQ.RIN4 and pEAQ.MPK4. …”
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