Functional characterization of gibberellic acid related genes from oil palm in Arabidopsis thaliana for potential role height regulation by Abdul Halim, Muhamad Afiq

“…The constructs were transformed into Arabidopsis via Agrobacterium-mediated transformation using floral dip method. …”
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Strigolactones in Sugarcane Growth and Development by Fenggang Zan, Zhuandi Wu, Wenzhi Wang, Xin Hu, Lu Feng, Xinlong Liu, Jiayong Liu, Liping Zhao, Caiwen Wu, Shuzhen Zhang, Jiawen Guo

“…Herein, the sugarcane variety ROC22 served as the receptor, the <i>Bar</i> gene served as a screening marker, and positive and negative fragments of the <i>ScD27.2</i> gene, upstream of strigolactones (SLs) biosynthesis genes driven by the <i>35S</i> promoter, were introduced by <i>Agrobacterium tumefaciens</i>-mediated transformation. …”
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Identification and Functional Analysis of SabHLHs in <i>Santalum album</i> L. by Ting Zhang, Xiaohong Chen, Yuping Xiong, Meiyun Niu, Yueya Zhang, Haifeng Yan, Yuan Li, Xinhua Zhang, Guohua Ma

“…We have also constructed pGreenII 62-SK-<i>SaMYC</i>1, pGreenII 0800-LUC-<i>SaSSy</i> and pGreenII 0800-<i>LUC</i>-<i>SaCYP736A167</i> via dual-luciferase fusion expression vectors and transformed them into <i>Nicotiana benthamiana</i> using an <i>Agrobacterium</i>-mediated method. The results showed that <i>SaMYC</i>1 was successfully combined with <i>SaSSy</i> or <i>SaCYP736A167</i> promoter and the <i>LUC/REN</i> value was 1.85- or 1.55-fold higher, respectively, than that of the control group. …”
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Gallbladder microbial species and host bile acids biosynthesis linked to cholesterol gallstone comparing to pigment individuals by Xinpeng Zhang, Junqing Hu, Junqing Hu, Junqing Hu, Yi Li, Jichao Tang, Kaijin Yang, Ayan Zhong, Yanjun Liu, Yanjun Liu, Tongtong Zhang, Tongtong Zhang, Tongtong Zhang

“…Microbial profiling revealed significant enrichment of Cutibacterium acnes and Microbacterium sp005774735 in gallstone and Agrobacterium pusense and Enterovirga sp013044135 in the bile of cholesterol GSD subjects. …”
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A comparison of three different delivery methods for achieving CRISPR/Cas9 mediated genome editing in Cichorium intybus L. by Umberto Salvagnin, Katharina Unkel, Thorben Sprink, Paul Bundock, Robert Sevenier, Milica Bogdanović, Slađana Todorović, Katarina Cankar, Johanna Christina Hakkert, Elio Schijlen, Ronald Nieuwenhuis, Maria Hingsamer, Veronika Kulmer, Michael Kernitzkyi, Dirk Bosch, Stefan Martens, Mickael Malnoy

“…CRISPR/Cas9 reagents were delivered by Agrobacterium-mediated stable transformation or transient delivery by plasmid or preassembled ribonucleic complexes (RNPs) using the same sgRNA. …”
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Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen quality by Xinyue Hu, Jie Li, Shuai Xin, Qingyuan Ouyang, Jialu Li, Lipeng Zhu, Jiwei Hu, Hua He, Hehe Liu, Liang Li, Shenqiang Hu, Jiwen Wang

“…Of the 24 genera present in semen: Brachybacterium, Brochothrix, Chryseobacterium, Kocuria, Marinococcus, Micrococcus, Rothia, Salinicoccus, and Staphylococcus originated from the environment; Achromobacter, Aerococcus, Corynebacterium, Desemzia, Enterococcus, Jeotgalicoccus, Pseudomonas, Psychrobacter, and Turicibacter originated from the cloaca; and Agrobacterium, Carnobacterium, Chelativorans, Devosia, Halomonas, and Oceanicaulis originated from the spermaduct. …”
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Terrestrial Compound Protein Replacing Dietary Fishmeal Improved Digestive Enzyme Activity, Immune Response, Intestinal Microflora Composition, and Protein Metabolism of Golden Pom... by Yongcai Ma, Zeliang Su, Fang Chen, Chao Xu, Kunsheng Jiang, Wenqiang An, Guanrong Zhang, Dizhi Xie, Shuqi Wang, Yewei Dong, Yuanyou Li

“…At the genus level, the beneficial bacterial strains Agrobacterium and Blantia of CP80 group were also similar to those of FM30 group (P<0.05), which were significantly higher than those of AP80 and PP80 groups, but the beneficial bacterial strains Bifidobacterium and Devosia of CP80 group were significantly higher than that in the other groups (P<0.05). …”
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Gastrointestinal symptoms of long COVID-19 related to the ectopic colonization of specific bacteria that move between the upper and lower alimentary tract and alterations in serum... by Deyu Zhang, Siyuan Weng, Chuanchao Xia, Yuqing Ren, Zaoqu Liu, Yudi Xu, Xiaoli Yang, Ruhao Wu, Lisi Peng, Liqi Sun, Jiaqi Zhu, Xuesong Liang, Yin Jia, Huaizhou Wang, Qian Chen, Dongtian Liu, Yi Chen, Honglei Guo, Xinwei Han, Zhendong Jin, Cui Chen, Xia Yang, Zhaoshen Li, Haojie Huang

“…Some of these bacteria, including Neisseria, Lautropia, and Agrobacterium, were highly related to differentially expressed serum metabolites with potential toxicity, such as 4-chlorophenylacetic acid, 5-sulfoxymethylfurfural, and estradiol valerate. …”
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Development of a dual immunochromatographic test strip to detect E2 and Erns antibodies against classical swine fever by Loc Tan Huynh, Loc Tan Huynh, Eun-Ju Sohn, Youngmin Park, Juhun Kim, Tomohiko Shimoda, Takahiro Hiono, Takahiro Hiono, Takahiro Hiono, Takahiro Hiono, Norikazu Isoda, Norikazu Isoda, Norikazu Isoda, Norikazu Isoda, Sung-Hee Hong, Ha-Na Lee, Yoshihiro Sakoda, Yoshihiro Sakoda, Yoshihiro Sakoda, Yoshihiro Sakoda

“…The effectiveness of ICS in evaluating the DIVA capability of two novel chimeric pestivirus vaccine candidates was assessed.MethodsRecombinant E2 or Erns protein was transiently expressed in the plant benthamiana using Agrobacterium tumefaciens. ICS was subsequently assembled, and goat anti-rabbit IgG and recombinant CSFV E2 or Erns protein were plated onto the nitrocellulose membrane as control and test lines, respectively. …”
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Integrative Analysis of the Wheat <i>PHT1</i> Gene Family Reveals A Novel Member Involved in Arbuscular Mycorrhizal Phosphate Transport and Immunity by Yi Zhang, Lizong Hu, Deshui Yu, Kedong Xu, Ju Zhang, Xiaoli Li, Pengfei Wang, Guo Chen, Zhihui Liu, Chunfeng Peng, Chengwei Li, Tiancai Guo

“…Furthermore, the mycorrhizal-specific/inducible genes are significantly induced in wheat roots at different stages of infection by colonizing fungi. Transient <i>Agrobacterium tumefaciens</i>-mediated transformation expression in onion epidermal cells showed that TaPT29-6A is a membrane-localized protein. …”
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Overexpression of Soybean <i>GmWRI1a</i> Stably Increases the Seed Oil Content in Soybean by Zhikun Wang, Yuanzhuo Wang, Ping Shang, Chao Yang, Mingming Yang, Jinxiu Huang, Baizheng Ren, Zhaohui Zuo, Qingyan Zhang, Wenbin Li, Bo Song

“…In this study, <i>GmWRI1a</i> was overexpressed in the soybean cultivar ‘Dongnong 50’ using <i>Agrobacterium</i>-mediated transformation to generate three transgenic lines with high seed oil contents. …”
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Wheat gibberellin oxidase genes and their functions in regulating tillering by Ting Wang, Junchang Li, Yumei Jiang, Jing Zhang, Yongjing Ni, Peipei Zhang, Ziping Yao, Zhixin Jiao, Huijuan Li, Lei Li, Yufan Niu, Qiaoyun Li, Guihong Yin, Jishan Niu

“…TaGA7ox-A1 overexpression transgenic wheat lines were obtained by Agrobacterium mediated transformation. Genomic PCR and first-generation sequencing demonstrated that the gene was integrated into the wheat genome. …”
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<i>GsRSS3L</i>, a Candidate Gene Underlying Soybean Resistance to Seedcoat Mottling Derived from Wild Soybean (<i>Glycine soja</i> Sieb. and Zucc) by Shuang Song, Jing Wang, Xingqi Yang, Xuan Zhang, Xiuli Xin, Chunyan Liu, Jianan Zou, Xiaofei Cheng, Ning Zhang, Yuxi Hu, Jinhui Wang, Qingshan Chen, Dawei Xin

“…Furthermore, <i>Nicotiana benthamiana</i> was subjected to an <i>Agrobacterium</i>-mediated transient expression assay to investigate the role of the five candidate genes in SMV resistance. …”
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Functional characterization of flower morphogenesis and senescence related genes using virus induced gene silencing technique in petunia hybrida by Siti Hajar Noor, Shaarani

“…Tandem pTRV2 constructs containing a Ph-CHS fragment and target gene fragment(s) are prepared and agrobacterium cultures possessing these constructs are infiltrated onto petunia leaves of 2 to 3 weeks old seedlings. …”
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An established protocol for generating transgenic wheat for wheat functional genomics via particle bombardment by Yaqiong Wang, Jian Zeng, Peipei Su, Hongyan Zhao, Li Li, Xiaoxue Xie, Qian Zhang, Ya’nan Wu, Ruibin Wang, Yufan Zhang, Boju Yu, Mingjie Chen, Yuesheng Wang, Guangxiao Yang, Guangyuan He, Junli Chang, Yin Li

“…More importantly, with over 20 years of experience in wheat transformation in our lab, we share the many technical details and recommendations and emphasize that the particle bombardment-mediated approach has fewer limitations in genotype dependency and vector construction when compared with the Agrobacterium-mediated methods. The particle bombardment-mediated method has been successful for over 30 wheat genotypes, from the tetraploid durum wheat to the hexaploid common wheat, from modern elite varieties to landraces. …”
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MucoRice-CTB line 19A, a new marker-free transgenic rice-based cholera vaccine produced in an LED-based hydroponic system by Yoshikazu Yuki, Yoshikazu Yuki, Yoshikazu Yuki, Shiho Kurokawa, Shiho Kurokawa, Kotomi Sugiura, Kotomi Sugiura, Koji Kashima, Shinichi Maruyama, Tomoyuki Yamanoue, Tomoyuki Yamanoue, Ayaka Honma, Mio Mejima, Natsumi Takeyama, Natsumi Takeyama, Masaharu Kuroda, Hiroko Kozuka-Hata, Masaaki Oyama, Takehiro Masumura, Rika Nakahashi-Ouchida, Rika Nakahashi-Ouchida, Rika Nakahashi-Ouchida, Kohtaro Fujihashi, Kohtaro Fujihashi, Kohtaro Fujihashi, Kohtaro Fujihashi, Takashi Hiraizumi, Eiji Goto, Hiroshi Kiyono, Hiroshi Kiyono, Hiroshi Kiyono, Hiroshi Kiyono, Hiroshi Kiyono, Hiroshi Kiyono

“…We previously established the selection-marker-free rice-based oral cholera vaccine (MucoRice-CTB) line 51A for human use by Agrobacterium-mediated co-transformation and conducted a double-blind, randomized, placebo-controlled phase I trial in Japan and the United States. …”
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Pathogenesis of acute hepatopancreatic necrosis disease caused by vibrio spp. in whiteleg shrimp Penaeus vannamei (Boone, 1931) by Muthukrishnan, Sarmila

“…Three types of quorum sensing (QS) signal molecules, namely, N-acyl-homoserine lactone (AHL), Autoinducer-2 (AI-2), and Cholerae autoinducer-1-like (CAI-1) molecules were then screened in the AHPND positive isolates using Agrobacterium tumefaciens KYC55, Vibrio campbellii JMH597, and V. campbellii JAF375 biosensors respectively. …”
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Reduction of cellulose crystallization by CRISPR/Cas9 -mediated mutagenesis in the P-CR domain of CESA4 subunit of white poplar (Populus alba L.) by Shahnoush Nayeri, Bahram Baghban Kohnehrouz, Seyed Abbas Rafat

“…The pulvini tTCL explants from the petioles of 4th nodes close to apical buds of P. alba L. were transformed using competent bacterial cells of Agrobacterium tumefaciens strain LBA4404 as described by Nayeri et al., 2022. …”
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