Downregulation of the NbNACa1 Gene Encoding a Movement-Protein-Interacting Protein Reduces Cell-to-Cell Movement of Brome mosaic virus in Nicotiana benthamiana by Masanori Kaido, Yosuke Inoue, Yoshika Takeda, Kazuhiko Sugiyama, Atsushi Takeda, Masashi Mori, Atsushi Tamai, Tetsuo Meshi, Tetsuro Okuno, Kazuyuki Mise

“…The binding of NbNACa1 to BMV MP was confirmed in vivo with an agroinfiltration-immunoprecipitation assay. To investigate the involvement of NbNACa1 in BMV multiplication, NbNACa1-silenced (GSNAC) transgenic N. benthamiana plants were produced. …”
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Development of Bottle Gourd Lines Resistant to Zucchini Yellow Mosaic Virus Using Ethyl Methanesulfonate Mutagenesis by Asma Mohammed Saeed Al-Kubati, Baoshan Kang, Liming Liu, Aqleem Abbas, Qinsheng Gu

“…Based on the M2 phenotypes, 106 M3 lines were selected and screened for resistance to ZYMV by mechanical inoculation and agroinfiltration. Nine M3 lines were resistant to ZYMV during three tests. …”
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A simple method of plant sectioning using the agarose embedding technique for screening intracellular green fluorescent protein by Nisa Ihsani, Fenny Martha Dwivany, Sony Suhandono

“…It is difficult to observe plant tissue sections transformed using the agroinfiltration method under a fluorescent microscope. …”
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Transcriptome Profiling of Tomato Fruit Development Reveals Transcription Factors Associated with Ascorbic Acid, Carotenoid and Flavonoid Biosynthesis. by Jie Ye, Tixu Hu, Congmei Yang, Hanxia Li, Mingze Yang, Raina Ijaz, Zhibiao Ye, Yuyang Zhang

“…This transcriptional correlation was confirmed by agroinfiltration mediated transient expression, which showed that most of the enzymatic genes in the ascorbic acid biosynthesis were regulated by the overexpression of each of the three transcription factors that were tested. …”
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Genomic structure and transcript analysis of the Rapid Alkalinization Factor (RALF) gene family during host-pathogen crosstalk in Fragaria vesca and Fragaria x ananassa strawberry. by Francesca Negrini, Kevin O'Grady, Marko Hyvönen, Kevin M Folta, Elena Baraldi

“…In silico analysis was used to identify distinct pathogen inducible elements upstream of the FanRALF3-1 gene. Agroinfiltration of strawberry fruit with deletion constructs of the FanRALF3-1 promoter identified a 5' region required for FanRALF3-1 expression in fruit, but failed to identify a region responsible for fungal induced expression.…”
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High-efficiency protein expression in plants from agroinfection-compatible <it>Tobacco mosaic virus </it>expression vectors by Lindbo John A

“…Co-introduction (by agroinfiltration) of this T-DNA along with a 35S promoter driven gene for the RNA silencing suppressor P19, from <it>Tomato bushy stunt virus </it>(TBSV) resulted in essentially complete infection of the infiltrated plant tissue with the TMV vector by 4 days post infiltration (DPI). …”
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Inhibition of the host proteasome facilitates papaya ringspot virus accumulation and proteosomal catalytic activity is modulated by viral factor HcPro. by Nandita Sahana, Harpreet Kaur, Basavaraj, Fatima Tena, Rakesh Kumar Jain, Peter Palukaitis, Tomas Canto, Shelly Praveen

“…Mutated forms of PRSV HcPro showed that the conserved KITC54 motif in the N-terminal domain of HcPro was necessary for its binding to PAA. Co-agroinfiltration assays demonstrated that HcPro expression mimicked the action of MG132, and facilitated the accumulation of bothtotal ubiquitinated proteins and viral/non-viral exogenous RNA in Nicotiana benthamiana leaves. …”
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Promotion of Bamboo mosaic virus accumulation in Nicotiana benthamiana by 5’→3’ exonuclease NbXRN4 by Cheng-Cheng eLee, Tzu-Ling eLin, Jhe-Wei eLin, Yu-Tsung eHan, Yu-Ting eHuang, Yau-Heiu eHsu, Menghsiao eMeng

“…To learn more about the replication complex of BaMV, a protein preparation enriched in the 155-kDa replication protein was obtained from Nicotiana benthamiana by a protocol involving agroinfiltration and immunoprecipitation. Subsequent analysis by SDS-PAGE and mass spectrometry identified a handful of host proteins that may participate in the viral replication. …”
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High-Level Systemic Expression of Conserved Influenza Epitope in Plants on the Surface of Rod-Shaped Chimeric Particles by Natalia V. Petukhova, Tatiana V. Gasanova, Peter A. Ivanov, Joseph G. Atabekov

“…This work intends to show some biological properties of these viruses following plant infections. Agroinfiltration experiments on Nicotiana benthamiana confirmed the efficient systemic expression of M2e peptides, and two point amino acid substitutions in recombinant CPs significantly influenced the symptoms and development of viral infections. …”
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Long-Term Potato Virus X (PVX)-Based Transient Expression of Recombinant GFP Protein in <i>Nicotiana benthamiana</i> Culture In Vitro by Yana Sindarovska, Mykola Kuchuk

“…Transient expression technology provides high yields of recombinant proteins in greenhouse-grown plants, but every plant must be artificially agroinfiltrated, and open greenhouse systems are less controlled. …”
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Recent Developments and Strategies for the Application of Agrobacterium-Mediated Transformation of Apple Malus × domestica Borkh by Susan Schröpfer, Janne Lempe, Ofere Francis Emeriewen, Henryk Flachowsky

“…In addition to the use of different tissues and media for transformation, agroinfiltration, as well as pre-transformation with a Baby boom transcription factor are notable successes that have improved transformation efficiency in apple. …”
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A High-Throughput Virus-Induced Gene-Silencing Vector for Screening Transcription Factors in Virus-Induced Plant Defense Response in Orchid by Hsiang-Chia Lu, Ming-Hsien Hsieh, Cheng-En Chen, Hong-Hwa Chen, Hsiang-Iu Wang, Hsin-Hung Yeh

“…This report describes our improvement of our previously reported orchid virus-induced gene silencing vector, pCymMV-pro60, with a modified Gateway cloning system which requires only one recombination and can be inoculated by agroinfiltration. We cloned 1,700 DNA fragments, including 187 predicted transcription factors derived from an established expression sequence tag library of orchid, into pCymMV-Gateway. …”
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The cucumber mosaic virus 1a protein regulates interactions between the 2b protein and ARGONAUTE 1 while maintaining the silencing suppressor activity of the 2b protein. by Lewis G Watt, Sam Crawshaw, Sun-Ju Rhee, Alex M Murphy, Tomás Canto, John P Carr

“…However, the 1a protein-2b protein interaction does not inhibit the ability of the 2b protein to inhibit silencing of reporter gene expression in agroinfiltration assays. The interaction between the CMV 1a and 2b proteins represents a novel regulatory system in which specific functions of a VSR are selectively modulated by another viral protein. …”
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Evaluation of the RNA Silencing Suppression Activity of Three Cherry Virus F-Encoded Proteins by Leonidas Lotos, Asimina Katsiani, Nikolaos I. Katis, Varvara I. Maliogka

“…The clones were used for transient expression experiments in <i>Nicotiana benthamiana</i> leaves, using co-agroinfiltration with a GFP-expressing vector. In both CPL and CPS, a rapid decrease in fluorescence was recorded, comparable to the negative control, whereas the MP of CVF retained the GFP’s fluorescence for a few days longer even though this was observed in a small number of infiltrated leaves. …”
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Partial Characterisation of Salmonella gallinarum Clinical Isolate and Expression of Its Antigenic Outer Membrane Protein C (OmpC) Gene In Planta by Ee Leen Pang, Hwei-San Loh

“…The project scope embarked on the identification of S. gallinarum clinical isolate, construction of expression cassette and delivery of constructs into Nicotiana benthamiana via agroinfiltration. The OmpC transcripts and proteins were detected successfully at the molecular weights of ~1.002 kbp and ~35 kDa, respectively. …”
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RCY1-Mediated Resistance to Cucumber mosaic virus Is Regulated by LRR Domain-Mediated Interaction with CMV(Y) Following Degradation of RCY1 by Hideki Takahashi, Hiromi Shoji, Sugihiro Ando, Yoshinori Kanayama, Tomonobu Kusano, Minoru Takeshita, Masashi Suzuki, Chikara Masuta

“…Following transient expression of RCY1-HA by agroinfiltration, the defense reaction was induced in N. benthamiana leaves infected with CMV(Y) but not in virulent CMV(B2)-infected N. benthamiana leaves transiently expressing RCY1-HA or CMV(Y)-infected N. benthamiana leaves transiently expressing HA-tagged RPP8 (RPP8-HA), which is allelic to RCY1. …”
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Control of anthocyanin and non-flavonoid compounds by anthocyanin-regulating MYB and bHLH transcription factors in Nicotiana benthamiana leaves by Nikolay Stoyanov Outchkourov, Carlos Alexandre Carollo, Victoria eGomez Roldan, Ric C.H. de Vos, Dirk J Bosch, Robert David Hall, Robert David Hall, Jules eBeekwilder

“…In this study we introduced Rosea1 (ROS1, a MYB type) and Delila (DEL, a bHLH type), into Nicotiana benthamiana leaves by agroinfiltration. ROS1 and DEL form a pair of well-characterized transcription factors from Snapdragon (Antirrhinum majus), which specifically induce anthocyanin accumulation when expressed in tomato fruit. …”
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Evaluation of Different RNA Extraction Methods from Agropatch Suppressor Assay for Small Quantities of Plant Tissue and Their Application for Analysis of Gene Expression by Amir G. SHAHRIARI, Aminallah TAHMASEBI

“…The agroinfiltration assay provides fast and efficient way to transiently express genes into plant cells by Agrobacterium tumefaciens. …”
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A new approach to protect tobacco plants from Cd contamination using the attenuated recombinant virus CMV△2b containing the PvSR2 gene by Juan Wu, Qiang Wang, Xiwu Zhu

“…N. benthamiana plants at six leaf stages were inoculated with plasmid-Agrobacterium (GV3101) containing pCB301-CMVFny209△2b-PvSR2, pCB301-CMVFny109 and pCB301-CMVFny309 by agroinfiltration. After the virus had been detected in the non-inoculated leaves of the plant inoculated with plasmid-agrobacterium corresponding to each treatment, the plant of each treatments in the experiment were inoculated with sap of plant leaves containing the virus. …”
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Nicotiana benthamiana as a production platform for artemisinin precursors. by Teun W J M van Herpen, Katarina Cankar, Marilise Nogueira, Dirk Bosch, Harro J Bouwmeester, Jules Beekwilder

“…<h4>Conclusion/significance</h4>This work shows that agroinfiltration of N. bentamiana can be used as a model to study the production of sesquiterpenoid pharmaceutical compounds. …”
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