Expression of HIV-1 antigens in plants as potential subunit vaccines by Tanzer Fiona L, Shephard Enid, Chakauya Ereck, Meyers Ann, Maclean James, Lynch Alisson, Williamson Anna-Lise, Rybicki Edward P

“…</p> <p>Conclusion</p> <p>Transient agroinfiltration was best for expression of all of the recombinant proteins tested, and p24 and p17/p24 were expressed at much higher levels than Pr55Gag. …”
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Synthesis and Characterization of a Full-Length Infectious cDNA Clone of <i>Tomato Mottle Mosaic Virus</i> by Liqin Tu, Shuhua Wu, Danna Gao, Yong Liu, Yuelin Zhu, Yinghua Ji

“…Both the model host <i>N. benthamiana</i> and the natural hosts tomato and pepper developed severe symptoms upon agroinfiltration with pToMMV, which had a strong infectivity. …”
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Characterization of <it>Vitis vinifera </it>NPR1 homologs involved in the regulation of <it>Pathogenesis-Related </it>gene expression by Walter Bernard, Mutterer Jerôme, Mestre Pere, Heitz Thierry, Le Henanff Gaëlle, Chong Julie

“…VvNPR1.1-GFP and VvNPR1.2-GFP fusion proteins were transiently expressed by agroinfiltration in <it>Nicotiana benthamiana </it>leaves, where they localized predominantly to the nucleus. …”
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Development of Rice Stripe Tenuivirus Minireplicon Reverse Genetics Systems Suitable for Analyses of Viral Replication and Intercellular Movement by Xiaoyan Zhang, Kai Sun, Yan Liang, Shuo Wang, Kaili Wu, Zhenghe Li, Zhenghe Li, Zhenghe Li

“…After delivery to Nicotiana benthamiana leaves via agroinfiltration, MR reporter gene expression was detected only when the codon-optimized large viral RNA polymerase protein (L) was coexpressed with the nucleocapsid (N) protein. …”
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Functional analysis of apple stem pitting virus coat protein variants by Xiaofang Ma, Ni Hong, Peter Moffett, Yijun Zhou, Guoping Wang

“…Agrobacterium mediated infiltration (Agroinfiltration) was used to express YFP-ASPV-CPs in Nicotiana. benthamiana and infect Nicotiana. occidental with PVX-ASPV-CPs in. …”
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Characterization of Cell-Death-Inducing Members of the Pectate Lyase Gene Family in Phytophthora capsici and Their Contributions to Infection of Pepper by Li Fu, Chunyuan Zhu, Xiaomeng Ding, Xiaoyan Yang, Paul F. Morris, Brett M. Tyler, Xiuguo Zhang

“…Here, we cloned 22 full-length pectate lyase (PcPL) genes from a highly aggressive strain of Phytophthora capsici SD33. Of these, PVX agroinfiltration revealed that 12 PcPL genes were found to be highly induced during infection of pepper by SD33 but the induction level was twofold less in a mildly aggressive strain, YN07. …”
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Identification of viral genes involved in pepper mottle virus replication and symptom development in Nicotiana benthamiana by Miao Fang, Jisuk Yu, Hae-Ryun Kwak, Kook-Hyung Kim, Kook-Hyung Kim, Kook-Hyung Kim

“…Effects of all mutants compared with 134 after inoculation onto N. benthamiana by agroinfiltration. Results from our study provide direct evidence that the helper component-proteinase (HC-Pro) and the nuclear inclusion protein b (NIb)-coat protein (CP) regions are involved in virus accumulation and symptom determinants. …”
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Editing Citrus Genome via SaCas9/sgRNA System by Hongge Jia, Jin Xu, Vladimir Orbović, Vladimir Orbović, Yunzeng Zhang, Nian Wang

“…Transient expression of SaCas9/sgRNA in Duncan grapefruit via Xcc-facilitated agroinfiltration showed it can successfully modify CsPDS and Cs2g12470. …”
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Late Blight Resistance Conferred by <i>Rpi-Smira2/R8</i> in Potato Genotypes In Vitro Depends on the Genetic Background by Eva Blatnik, Marinka Horvat, Sabina Berne, Miha Humar, Peter Dolničar, Vladimir Meglič

“…Ten <i>R8</i> potato genotypes were obtained using stepwise marker-assisted selection, and agroinfiltration of the avirulence effector gene <i>Avr4</i>. …”
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Two NIS1-like proteins from apple canker pathogen (Valsa mali) play distinct roles in plant recognition and pathogen virulence by Jiajun Nie, Wenjing Zhou, Yonghui Lin, Zhaoyang Liu, Zhiyuan Yin, Lili Huang

“…Both of these two proteins were predicted to be secreted. Using agroinfiltration, we found that VmNIS1 induced intense cell death, whereas VmNIS2 suppressed INF1 elicitin-triggered cell death in Nicotiana benthamiana. …”
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Development of a Full-Length Infectious cDNA Clone of the Grapevine Berry Inner Necrosis Virus by Xudong Fan, Zunping Zhang, Fang Ren, Guojun Hu, Chen Li, Baodong Zhang, Yafeng Dong

“…Moreover, the cDNA clone was agroinfiltrated into “Beta” and “Thompson Seedless” grapevine plantlets, and the inoculated grapevines exhibited leaf chlorotic mottling and ringspot during the two years of observation. …”
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<i>Pseudomonas syringae</i> pv. <i>actinidiae</i> Effector HopAU1 Interacts with Calcium-Sensing Receptor to Activate Plant Immunity by Jinlong Zhang, Mingxia Zhou, Wei Liu, Jiajun Nie, Lili Huang

“…Here, we successfully identified a T3SS effector HopAU1 which had no virulence contribution to <i>Psa</i>, but could, however, induce cell death and activate a series of immune responses by agroinfiltration in <i>Nicotiana benthamiana</i>, including elevated transcripts of immune-related genes, accumulation of reactive oxygen species (ROS), and callose deposition. …”
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Transcriptional reprogramming caused by the geminivirus Tomato yellow leaf curl virus in local or systemic infections in Nicotiana benthamiana by Mengshi Wu, Xue Ding, Xing Fu, Rosa Lozano-Duran

“…Results In order to gain insight into how different the transcriptional landscapes of TYLCV-infected cells or whole tissues of TYLCV-infected plants might be, here we compare the transcriptional changes in leaf patches infected with TYLCV by agroinfiltration or in systemic leaves of TYLCV-infected plants in Nicotiana benthamiana. …”
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Somatic Embryogenesis and <i>Agrobacterium</i>-Mediated Gene Transfer Procedures in Chilean Temperate Japonica Rice Varieties for Precision Breeding by Marion Barrera, Blanca Olmedo, Carolina Zúñiga, Mario Cepeda, Felipe Olivares, Ricardo Vergara, Karla Cordero-Lara, Humberto Prieto

“…Reporter mRNA was produced as early as 3 d after agroinfiltration, and stable expression of the protein was observed along the complete process. …”
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Transient expression and purification of β-caryophyllene synthase in Nicotiana benthamiana to produce β-caryophyllene in vitro by Saraladevi Muthusamy, Ramesh R. Vetukuri, Anneli Lundgren, Suresh Ganji, Li-Hua Zhu, Peter E. Brodelius, Selvaraju Kanagarajan

“…Subsequently, the plasmid was transferred into the Agrobacterium and agroinfiltrated into N. benthamiana leaves. The AaCPS1 expression was analyzed by quantitative PCR at different time points after agroinfiltration. …”
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Turnip Mosaic Virus Coat Protein Deletion Mutants Allow Defining Dispensable Protein Domains for ‘in Planta’ eVLP Formation by Carmen Yuste-Calvo, Pablo Ibort, Flora Sánchez, Fernando Ponz

“…In order to identify exclusively those domains involved in protein–protein interactions within the particle, the analysis was performed by agroinfiltration “in planta”, followed by the assessment of CP accumulation in leaves and the assembly of virus-like particles lacking nucleic acids, also known as empty virus-like particles (eVLP). …”
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Evaluation of Parameters Affecting <i>Agrobacterium</i>-Mediated Transient Gene Expression in Industrial Hemp (<i>Cannabis sativa</i> L.) by Tasnim Mohammad, Rishikesh Ghogare, Lauren B. Morton, Amit Dhingra, Shobha Potlakayala, Sairam Rudrabhatla, Sarwan K. Dhir

“…In this study, the parameters for an agroinfiltration system in hemp, which applies to the stable transformation method, were optimized. …”
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Profiles of Cytokinins Metabolic Genes and Endogenous Cytokinins Dynamics during Shoot Multiplication In Vitro of <i>Phalaenopsis</i> by Yuan-Yuan Li, Zhi-Gang Hao, Shuo Miao, Xiong Zhang, Jian-Qiang Li, Shun-Xing Guo, Yung-I Lee

“…Overexpression of <i>PaIPT1</i> and <i>PaLOG2</i> by agroinfiltration assays resulted in increased CK-ribosides levels in tobacco leaves, while overexpression of <i>PaCKX4</i> resulted in decreased CK-ribosides levels. …”
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LbCas12a-D156R Efficiently Edits <i>LOB1</i> Effector Binding Elements to Generate Canker-Resistant Citrus Plants by Hongge Jia, Yuanchun Wang, Hang Su, Xiaoen Huang, Nian Wang

“…We first constructed GFP-p1380N-ttLbCas12a:LOBP, which was shown to be functional via Xcc-facilitated agroinfiltration in Pummelo (<i>Citrus maxima</i>) leaves. …”
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Expression of the Biologically Active Insulin Analog SCI-57 in Nicotiana Benthamiana by Adriana Muñoz-Talavera, Miguel Ángel Gómez-Lim, Luis A. Salazar-Olivo, Jörg Reinders, Katharina Lim, Abraham Escobedo-Moratilla, Alberto Cristian López-Calleja, María Cristina Islas-Carbajal, Ana Rosa Rincón-Sánchez

“…Expression of the insulin analog in agroinfiltrated plants was confirmed by SDS-PAGE, RP-HPLC, and MS. …”
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