Improving agroinfiltration-based transient gene expression in Nicotiana benthamiana by Karlah Norkunas, Robert Harding, James Dale, Benjamin Dugdale

Subjects: “…Agroinfiltration…”
Get full text

Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants by Maninder Kaur, Pooja Manchanda, Anu Kalia, Farah K. Ahmed, Eugenie Nepovimova, Kamil Kuca, Kamel A. Abd-Elsalam

“…This review aims to provide information on <i>Agrobacterium</i>-mediated transformation and implementation of agroinfiltration with GE to widen the horizon of targeted genome editing before a stable genome editing approach. …”
Get full text

Agromonas: a rapid disease assay for Pseudomonas syringae growth in agroinfiltrated leaves by Buscaill, P, Sanguankiattichai, N, Lee, YJ, Kourelis, J, Preston, G, van der Hoorn, RAL

“…Here we present an easy method called agromonas, where we quantify&nbsp;<em>P. syringae</em>&nbsp;growth in agroinfiltrated leaves of&nbsp;<em>Nicotiana benthamiana</em>&nbsp;using a cocktail of antibiotics to select&nbsp;<em>P. syringae</em>&nbsp;on plates. …”

Agroinfiltration of Cauliflower mosaic virus Gene VI Elicits Hypersensitive Response in Nicotiana Species by Karuppaiah Palanichelvam, Anthony B. Cole, Monir Shababi, James E. Schoelz

“…To prove conclusively that W260 gene VI is responsible, we inserted gene VI of W260 and D4 into the Agrobacterium tumefaciens binary vector pKYLX7. Agroinfiltration of these constructs into the leaves of N. edwardsonii and N. clevelandii revealed that gene VI of W260 elicited HR in N. edwardsonii 4 to 5 days after infiltration and cell death in N. clevelandii approximately 9 to 12 days after infiltration. …”
Get full text

Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene. by Aya Bridgeland, Sudip Biswas, Nikolaos Tsakirpaloglou, Michael J Thomson, Endang M Septiningsih

“…Sanger sequencing of DNA from transformed protoplasts and agroinfiltrated cowpea leaves revealed several large deletions in the target sequences. …”
Get full text

Transient Transformation of Potato Plant (Solanum tuberosum L.) Granola Cultivar Using Syringe Agroinfiltration by Yesy John Mba’u, Iriawati Iriawati, Ahmad Faizal

Subjects: “…Agroinfiltration…”
Get full text

In planta Female Flower Agroinfiltration Alters the Cannabinoid Composition in Industrial Hemp (Cannabis sativa L.) by Michihito Deguchi, Seema Dhir, Shobha Potlakayala, Sarwan Dhir, Wayne R. Curtis, Sairam Rudrabhatla

Subjects: “…agroinfiltration…”
Get full text

Optimized Agroinfiltration and Virus-Induced Gene Silencing to Study Ve1-Mediated Verticillium Resistance in Tobacco by Zhao Zhang, Emilie Fradin, Ronnie de Jonge, H. Peter van Esse, Patrick Smit, Chun-Ming Liu, Bart P. H. J. Thomma

Get full text

Agroinfiltration by Cytokinin-Producing Agrobacterium sp. Strain GV3101 Primes Defense Responses in Nicotiana tabacum by Arsheed Hussain Sheikh, Badmi Raghuram, Lennart Eschen-Lippold, Dierk Scheel, Justin Lee, Alok Krishna Sinha

Get full text

Agroinfiltration reduces ABA levels and suppresses Pseudomonas syringae-elicited salicylic acid production in Nicotiana tabacum. by Arantza Rico, Mark H Bennett, Silvia Forcat, Wei E Huang, Gail M Preston

Get full text

Agrobacterium-mediated transformation by using immerse and agroinfiltration method for genome editing of orchids with CRISPR/Cas9 by Subchan, A. N., Nopitasari, S., Setiawati, Y., Zubaidah, L., Nikmah, N., Lawrie, M. D., Sasongko, A .B., Matsumoto, S., Yoshioka, Y., Semiarti, E.

“…In contrast, all positive agroinfiltration samples carried T-DNA (100%). From this it can be concluded that the agroinfiltration method has a high potential to be used for gene transfer in orchids.…”

Agroinfiltration reduces ABA levels and suppresses Pseudomonas syringae-elicited salicylic acid production in Nicotiana tabacum by Rico, A, Bennett, M, Forcat, S, Huang, W, Preston, G

Agroinfiltration reduces ABA levels and suppresses Pseudomonas syringae-elicited salicylic acid production in Nicotiana tabacum by Rico, A, Bennett, M, Forcat, S, Huang, W, Preston, G

Agroinfiltration reduces ABA Levels and suppresses Pseudomonas syringae-elicited salicylic acid production in Nicotiana tabacum by Rico, A, Bennett, M, Forcat, S, Huang, W, Preston, G

The transient expression of coat protein of Foot and Mouth Disease Virus (FMDV) in spinach (Spinacia oleracea) using Agroinfiltration by Maziar Habibi, Saeid Malekzadeh-Shafaroudi, Hassan Marashi, Nasrin Moshtaghi, Mohammadreza Nasiri, Saeed Zibaee

Subjects: “…agroinfiltration…”
Get full text

A Tobacco Syringe Agroinfiltration-Based Method for a Phytohormone Transporter Activity Assay Using Endogenous Substrates by Jiangzhe Zhao, Min Ju, Jiayun Qian, Mengyuan Zhang, Ting Liu, Kewei Zhang

Subjects: Get full text

Scalable agroinfiltration-based production of SARS-CoV-2 antigens for use in diagnostic assays and subunit vaccines. by Jordan Demone, Mariam Maltseva, Maryam Nourimand, Mina Nasr-Sharif, Yannick Galipeau, Emilio I Alarcon, Marc-André Langlois, Allyson M MacLean

“…Agroinfiltration is a method used in biopharming to support plant-based biosynthesis of therapeutic proteins such as antibodies and viral antigens involved in vaccines. …”
Get full text

The transcriptome, extracellular proteome and active secretome of agroinfiltrated Nicotiana benthamiana uncover a large, diverse protease repertoire. by Grosse-Holz, F, Kelly, S, Blaskowski, S, Kaschani, F, Kaiser, M, van der Hoorn, R

“…Proteolysis may be linked to an immune response to agroinfiltration, but understanding of the response to agroinfiltration is limited. …”

An Improved Syringe Agroinfiltration Protocol to Enhance Transformation Efficiency by Combinative Use of 5-Azacytidine, Ascorbate Acid and Tween-20 by Huimin Zhao, Zilong Tan, Xuejing Wen, Yucheng Wang

Subjects: Get full text

Optimization of agroinfiltration in Pisum sativum provides a new tool for studying the salivary protein functions in the pea aphid complex by Endrick Guy, Hélène Boulain, Yoann Aigu, Charlotte Le Pennec, Khaoula Chawki, Stéphanie Morlière, Kristina schaedel, Grit Kunert, Jean-Christophe Simon, Akiko Sugio

Subjects: Get full text