Isolation, Characterization and Genetic Linkage Mapping of Microsatellite Markers in Mystus Nemurus par Hoh, Boon Peng

“…Two methodologies were used to isolate the microsatellite loci in M. nemurus, namely Random Amplified Hybridizing Microsatellites (RAHMs) and 5' anchored Polymerase Chain Reaction (5' anchored PCR). …”
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Molecular Cloning and Gene Expression Analysis of the Phospholipase B Genes of Non-Albzcans Candzda Species par Cheang, Pey Shyuan

“…The second and third pairs of degenerate primers amplified hgments corresponding to the 3' proximal end region of the putative PLB genes from the ATCC strains as well as clinical isolates of C. parapsilosis and C. …”
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Structural Gene Characterization And Development Of Real-Time Pcr Detection Method For Infectious Bronchitis Virus par Mohamed Zulperi, Zarirah

“…The structural genes were amplified using gene-specific primers in reverse-transcriptase polymerase chain reaction (RT-PCR) and cloned into TOPO TA cloning vector. …”
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Characterisation and rescue of a recombinant newcastle disease virus strain AF2240-I par Kavitha, Murulitharan

“…A frameshift was also observed between the amplified matrix (M) gene of AF2240-I and the published M gene sequence of AF2240. …”
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Vitamin D receptor, interleukin 12B and interleukin 23R gene polymorphisms and risk of chronic plaque psoriasis among patients in a Malaysian hospital par Matnor, Nur Syazana

“…Socio-demographic data was recorded for both groups with additional Psoriasis Area Severity Index (PASI) and Dermatology Life Quality Index (DLQI) from psoriasis patients. Extracted DNA was amplified by polymerase chain reaction (PCR) reaction and genotyped for SNP by restriction fragment length polymorphism (RFLP) analysis. …”
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Detection of tungro disease in rice leaf in relation to nitrogen level using laser light backscattering imaging par Bachik, Nur Azizah

“…The PCR result showed that the expected deoxyribonucleic acid (DNA) size (500 base pairs (bp)) of RTBV was amplified for inoculated rice plants. The LLBI parameters showed that all parameters except maximum intensity, minimum intensity and ratio were influenced by INO treatments while only minimum intensity was influenced by N treatments. …”
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Development of Novel Primers and Probes Based on its 2 Region Using Nested-PCR and DNA Hybridization Array for Candida Species Identification par Chieng, David Ching Soo

“…For nested-PCR, the universal fungal primers, ITS 3 and ITS 4 were used to amplify the portion of the 5.8S and 28S rDNA, and the ITS 2 region of Candida species. …”
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Fingerprinting the painted storks (Mycteria leucocephala pennant), milky storks (Mycteria cinerea raffles) and their suspicious hybrids in a zoo in Malaysia par Yee, Yoke Sim

“…DNA was extracted from a disc of FTA cards (3 mm), and PCR was run with RAPDs (random amplified polymorphism DNA), ISSRs (inter-specific sequence repeat), and cross species microsatellites, electrophoresed on agarose gels and stained with ethidium bromide. …”
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Molecular epidemiology, risk factors of zoonotic enteric protozoa and genetic diversity of Blastocystis infecting cattle in Peninsular Malaysia par Abdullah, Donea Abdulrazak

“…There was a considerably high level of genetic variability among the Blastocystis subtypes whereby a total of 117 haplotypes were amplified, with high nucleotide diversity. The present study constitutes the first attempt to apply molecular detection techniques to determine epidemiological risk factors for enteric protozoa infections among cattle in Malaysia. …”
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Influence of dietary ratios of n-6: n-3 fatty acid on gene expression, fatty acid profile in liver and breast muscle tissues, serum lipid profile, and immunoglobulin in broiler chi... par Abdulla, Nazim Rasul, Loh, Teck Chwen, Foo, Hooi Ling, Alshelmani, Mohamed Idris, Akit, Henny

“…The ratios of C22:6n-3, C18:3n-3, and C22:5n-3 in liver and breast muscle tissue amplified in response to a decrease in the proportion of n-6: n-3 FAR in diets. …”
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Species Classification and Molecular Studies of Bile Salt Hydrolase (BSH) Gene in Bifidobacterium Spp par Mustafa, Shuhaimi

“…A protocol for the rapid fingerprinting technique of Bifidobacterium strains and other pro biotic microorganisms based on randomly amplified polymorphic DNA (RAPD) has been developed. …”
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Evidence of West Nile virus infection in migratory and resident wild birds in west coast of peninsular Malaysia par Mohd Yuseri, Nur Ain Najwa, Abd Rahaman, Nor Yasmin, Abdul Rahman, Omar, Arshad, Siti Suri, Abu, Jalila, Mohammed, Hussni O., Kumar, Kiven, Shih, Keng Loong, Rovie-Ryan, Jeffrine J., Mohd Kharip Shah, Ahmad Khusaini

“…West Nile virus (WNV) is a zoonotic mosquito-borne flavivirus that is harbored and amplified by wild birds via the enzootic transmission cycle. …”
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Development of an Rt Nested PCR-Elisa Diagnostic Test for the Detection of Newcastle Disease Virus par Kho, Chiew Ling

“…To facilitate the detection of the peR product, the amplified peR product was then subjected to a colorimetric detection method using ELIS A where the labeled peR product was captured in a streptavidin coated microtiter plate and was detected by using anti-digoxigenin-peroxidase enzyme conjugate and 2,2'-azinodiethylbenzothiazolinesulfonic acid (ABTS) as the substrate. …”
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Synergistic effect of local endothelial shear stress and systemic hypercholesterolemia on coronary atherosclerotic plaque progression and composition in pigs par Koskinas, Konstantinos C., Chatzizisis, Yiannis S., Coskun, Ahmet U., Baker, Aaron B., Jarolim, Petr, Antoniadis, Antonios, Edelman, Elazer R., Stone, Peter H., Feldman, Charles L., Papafaklis, Michail

“…Conclusions This study redefines the principle concept that “low” ESS promotes coronary plaque growth and vulnerability by demonstrating that: (i.) the pro-atherogenic threshold of low ESS is not uniform, but cholesterol-dependent; and (ii.) the atherogenic effects of local low ESS are amplified, and the athero-protective effects of higher ESS may be outweighed, by increasing cholesterol levels. …”
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Assessing the impacts of 1.5 °C global warming – simulation protocol of the Inter-Sectoral Impact Model Intercomparison Project (ISIMIP2b) par Frieler, Katja, Lange, Stefan, Piontek, Franziska, Reyer, Christopher P. O., Schewe, Jacob, Warszawski, Lila, Zhao, Fang, Chini, Louise, Denvil, Sebastien, Geiger, Tobias, Halladay, Kate, Hurtt, George, Mengel, Matthias, Murakami, Daisuke, Ostberg, Sebastian, Popp, Alexander, Riva, Riccardo, Stevanovic, Miodrag, Suzuki, Tatsuo, Volkholz, Jan, Burke, Eleanor, Ciais, Philippe, Ebi, Kristie, Eddy, Tyler D., Elliott, Joshua, Galbraith, Eric, Gosling, Simon N., Hattermann, Fred, Hickler, Thomas, Hinkel, Jochen, Hof, Christian, Huber, Veronika, Jägermeyr, Jonas, Krysanova, Valentina, Marcé, Rafael, Schmied, Hannes Müller, Mouratiadou, Ioanna, Pierson, Don, Tittensor, Derek P., Vautard, Robert, van Vliet, Michelle, Biber, Matthias F., Betts, Richard A., Bodirsky, Benjamin Leon, Deryng, Delphine, Frolking, Steve, Jones, Chris D., Lotze, Heike K., Lotze-Campen, Hermann, Sahajpal, Ritvik, Thonicke, Kirsten, Tian, Hanqin, Yamagata, Yoshiki, Emanuel, Kerry Andrew

“…With the aim of providing the scientific basis for an aggregation of impacts across sectors and analysis of cross-sectoral interactions that may dampen or amplify sectoral impacts, the protocol is designed to facilitate consistent impact projections from a range of impact models across different sectors (global and regional hydrology, lakes, global crops, global vegetation, regional forests, global and regional marine ecosystems and fisheries, global and regional coastal infrastructure, energy supply and demand, temperature-related mortality, and global terrestrial biodiversity).…”
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Semiconducting polymer nanoparticles for multimodal biophotonics par Jiang, Yuyan

“…This study thus proposes a molecular strategy to devise simple yet amplified photosensitizers to improve traditional PDT. …”
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Genetic polymorphism and natural selection in the C-terminal 42 kDa region of merozoite surface protein-1 (MSP-1) among Plasmodium knowlesi samples from Malaysia par Rajoo, Yamuna, Choy, Seow Huey, Yap, Nan Jiun, Vythilingam, Indra, Hoh, Boon Peng, Goh, Xiang Ting, Muslim, Azdayanti, Ngui, Romano, William, Timothy, Yeo, Tsin Wen, Lim, Yvonne Ai-Lian

“…The region flanking PkMSP-142 was amplified by PCR and directly sequenced. Genetic diversity, haplotype diversity, population genetic differentiation and natural selection were determined in order to study the polymorphic characteristic of PkMSP-142. …”
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Effects of ozone-vegetation interactions on meteorology and air quality in China using a two-way coupled land-atmosphere model par Zhu, Jiachen, Tai, Amos P. K., Yim, Steve Hung Lam

“…We also found that the meteorological changes further induce a 2-6gppb increase in O3 concentration in northern and south-central China mainly due to enhanced isoprene emission following increased air temperature, demonstrating that O3-vegetation interactions can lead to strong positive feedback that can amplify O3 pollution in China. Our findings emphasize the importance of considering the effects of O3 damage and O3-vegetation interactions in air quality simulations, with ramifications for both air quality and forest management.…”
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Opposing effects on regulated insulin secretion of acute vs chronic stimulation of AMP-activated protein kinase par Nguyen-Tu, Marie-Sophie, Harris, Joseph, Martinez-Sanchez, Aida, Chabosseau, Pauline, Hu, Ming, Georgiadou, Eleni, Pollard, Alice, Otero, Pablo, Lopez-Noriega, Livia, Leclerc, Isabelle, Sakamoto, Kei, Schmoll, Dieter, Smith, David M., Carling, David, Rutter, Guy A.

“…Although abolished in the absence of AMPK, the effects of 991 were observed in the absence of the upstream kinase, liver kinase B1, further implicating ‘amplifying’ pathways. In marked contrast, chronic activation of AMPK, either globally or selectively in the beta cell, achieved using a gain-of-function mutant, impaired insulin release in vivo (p<0.05 at 15 min following i.p. injection of 3 mmol/l glucose) and in vitro (p<0.01 following incubation of islets with 17 mmol/l glucose), and lowered glucose tolerance (p<0.001). …”
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Evaporation from nanoporous membranes for high heat flux thermal management par Hanks, Daniel Frank

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