Chimeric lipase for understanding the role of key residues governing pH profiles by Wan Hasan, Wan Atiqah Najiah

“…Using the gene shuffling method, fragments of lipase gene from Staphylococcus hyicus lipase and Staphylococcus epidermidis lipase were amplified and purified before being treated with restriction enzyme MseI which cut each lipase at several sites. …”
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Application of Comparative Genomic Hybridization and Fluorescent in Situ Hybridization Techniques on Human Glioma Cell Lines Treated with Bis[S-Methyl-A-N-(2 - Furylmethylketone) D... by Abu Bakar, Suhaili

“…After treatment with SMDB-Cd, p53 level was observed to be amplified in T98G. Increasing of p53 level may have been induced by the action of SMDB-Cd on the cells that inhibit cell growth and lead to cell damage. …”
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Optimization of Polymerase Chain Reaction-Based Screening Techniques for the Detection Of Fragile X Syndrome by Alimat, Sharizah

“…The results demonstrated that almost all the suspected individuals show the fblly mutated fragments with more than 600 bp and for the premutation stage, higher than 500 bp fragments were amplified. Only a few suspected individuals showed the normal fragment amplification (152 bp to 265 bp). …”
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Anti-leukaemic and chemotherapy supportive effects of berberis vulgaris l. Fruit crude extract on in vitro and in vivo models by Saedi, Tayebeh Azam

“…Moreover, real time PCR was used to amplify mRNA and determine the expression of some specific related genes in leukaemia. …”
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Mitogenic activity of mesenchymal stem cells on hematopoietic stem cells via cellular analysis and gene expression by Mohadeseh, Hashem Boroojerdi

“…Conclusion: The present study support the notion that the human umbilical cord derived MSC as potential feeder cells that could preserve and amplify the number of human cord blood derived-HSC. …”
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Efficiency and determinants of entry into Malaysian palm oil refinery industry by Choo, Sze Yi

“…It is also essential for refineries to boost productivity, efficiency and competitiveness in both domestic and global markets through adoption of advanced technologies in machineries, equipments and refinery plants. Refineries ought to amplify their production scale to attain economies of scale in production to enjoy lower cost advantage and higher utilisation rate. …”
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Identification and functional analysis of cyotochrome P450 from Bacillus lehensis G1 by Ang, Swi See

“…The CYP107CB2 gene was isolated and amplified using PCR and the CYP107CB2 protein was over-expressed in E. …”
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Antigenic analysis of outer membrane protein of Vibrio species and development of versatile recombinant vhDnaJ vaccine against vibriosis by Mursidi, Fathin Amirah

“…The vhDnaJ gene was amplified and cloned in pET-32 Ek/LIC vector and expressed in host BL21 (DE3) Escherichia coli. …”
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Design and Development of a Compact and Vialess Microstrip Ultra-Wideband Bandpass Filter by Razalli, Mohammad Shahrazel

“…They are built together with other components such as mixers, amplifiers, oscillators and switches. Filters work by blocking the unwanted signals and allowing the desired signals through them before feeding it into other components or devices. …”
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Effect of palm oil mill final discharge on the physicochemical characteristics and bacterial community of river water by Tun Mohd Salim, Wan Nor Salmah

“…In addition, bacterial community structure was analyzed by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified region of 16S rDNA from two different points of Marong River and palm oil mill final discharge. …”
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Diversity of soil green algae in an oil palm plantation at Sungai Asap, Sarawak, Malaysia by Lim, Chin Tsong

“…Total soil microorganism DNA was extracted and soil green algae 18S rDNA was amplified using ChloroF and ChloroR primers set. Denaturing gradient gel electrophoresis (DGGE) was used to study the diversity of soil green algae communities of the oil palm plantation and the nearby secondary forest at Sungai Asap, Sarawak. …”
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Isolation, Screening and Molecular Cloning Studies of Xylanase Producing Bacillus Pumilus Strains by Hamzah, Ainon

“…A complete DNA sequence of the xylanase gene was amplified by polymerase chain reaction and cloned into E. coli INVaF' using pCRII cloning vector. …”
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Evaluation, characterisation and modelling of Aspergillus flavus in aflatoxin production in peanuts along the supply chain by Mahror, Norlia

“…Aflatoxin biosynthesis genes namely aflR, aflP (omtA), aflD (nor-1), aflM (ver-1), and aflC (pksA) were detected in all aflatoxigenic A. flavus while the non-aflatoxigenic A. flavus failed to amplify at least one of the genes that was tested. …”
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Detection of polymorphisms and fecundity determination in Axis axis Erxleben, Rusa timorensis Blainville and Rusa unicolor Kerr raised in Lenggong, Perak, Malaysia by Yahaya, Muhammad Sanusi

“…Following the discovery of chromosomal aberrations, DNA was obtained from 45 individuals to amplify the protein coding regions of the fecundity genes, through high fidelity PCR. …”
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Development of a dna-based method for simultaneous detection of acinetobacter baumannii, antimicrobial resistance genes and its genotypes by dna fingerprinting by Ee, Chan Shiao

“…Speciation on Acinetobacter isolates recovered from 115 blood specimens collected over 24-month period with amplified ribosomal DNA restriction analysis was performed. …”
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The effect of budesonide and 5-azacytidine on the levels of methylation at the CpG islands of human choline kinase alpha (ckα) and beta (ckβ) promoter by Rahman, Muhammad Al Bunyamin Abdul

“…Seven regions of out fourteen regions of the promoter CpG islands were successfully amplified by producing PCR products at their expected sizes. …”
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Isolation of leptospira spp. and serological diagnoses in patients with acute febrile illness in Hospital Universiti Sains Malaysia by Mohamad, Amira Wahida

“…The positive cultures were amplified and identified by PCR on 16S rRNA gene by sequencing. …”
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Bacterial community (16S rRNA analysis) of the tomato calyx and pedicel over time, after ozone treatment or not by Macdonald, A, Holden, N, Preston, G

“…A PCR negative control was collected by PCR-amplifying sterile water only (NC.2). PCR products were cleaned up according to the Illumina protocol, however the beads used were Macherey-Nagel NucleoMag NGS Clean-up and Size Select (FisherScientific, USA, product code: 15889167). …”

Adiposity impacts cognitive function in Asian populations: an epidemiological and Mendelian Randomization study by Mina, Theresia, Yew, Yik Weng, Ng, Hong Kiat, Sadhu, Nilanjana, Wansaicheong, Gervais, Dalan, Rinkoo, Low, Dorrain Yan Wen, Lam, Benjamin Chih Chiang, Riboli, Elio, Lee, Eng Sing, Ngeow, Joanne, Elliott, Paul, Griva, Konstadina, Loh, Marie, Lee, Jimmy, Chambers, John

“…Asia has a high prevalence of both obesity and metabolic disease, potentially amplifying the future burden of dementia in the region. …”
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Parametric subharmonic instability of internal gravity wave beams by Karimi, Hussain H. (Hussain Habibullah)

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