Analysis of Cholera Epidemics in Sarawak From 1994-2003 and Molecular Characterization of Vibrio Cholerae Isolated from the Outbreaks in Malaysia by Benjamen, Patrick Guda

“…The ctx genes distinguish these epidemic serotypes from the non-01 serotypes. Randomly amplified polymorphic DNA (RAPD) analysis was used to analyze the genetic relatedness of all the 32 V. …”
Get full text

Construction Of Recombinant Escherichia Coli Bad85 For The Production Of High Purity L-Lactic Acid by Tengku Zainal Mulok, Tengku Elida

“…Primers EF.f (forward) and EF.r (reverse) were designed based on published gene sequence of L-ldh, and the PCR amplified L-ldh gene was cloned into TOPO TA cloning vector. …”
Get full text

Genetic Diversity of Boleophthalmus Boddarti and Other Malaysian Gobies by Mohammadi, Mehdi

“…Using the Discriminate Function Analysis (DFA), the conventional morphometric discriminate the populations of B. boddarti into 2 groups while Truss morphometrics into 3 groups, whereby geographically closer populations were grouped together. Of the 29 Randomly Amplified Polymorphic DNA (RAPD) primers tested, only 12 primers gave clear bands and showed polymorphisms. …”
Get full text

Genetic characterisations of four goat breeds in Malaysia assessed using microsatellites by Abu Bakar, Amie Marini

“…Thirty out of the 37 microsatellite loci were polymorphic in all four goat breeds,while six loci were monomorphic and one locus failed to amplify for the Boer goat breed suggesting that it was monomorphic for the null allele. …”
Get full text

Effects Of Recombinant Lactococcus Lactis And Bacteriocin Ul4 In The Protection Of Tilapia (Oreochromis Niloticus) Against Aeromonas Hydrophila by Karunakaramoorthy, Anuradha

“…For the constructions of recombinant L. lactis, a 250 bp domain 1 and 750 bp domain 4 of aerolysin produced by A. hydrophila were amplified by PCR and individually cloned into pNZ8048. …”
Get full text

Cyclic voltammetry electrodeposition and modification of silver nanoparticles-reduced graphene oxide electrode for immunosensing by Jamil, Asilah

“…The electrochemical conductivity were enhanced significantly by 40-fold, resulting in a notable amplified electrical signal for the detection of H2O2. …”
Get full text

Structure and functions of a metallo-beta-lactamase- like hypothetical protein bleg1_2437 from alkali-tolerant soil bacterium Bacillus lehensis G1 by Tan, Soo Huei

“…The ORF of Bleg1_2437 with approximately 633 nucleotides was amplified by PCR and cloned into pET-32(b) to form pET- 32(b)::Bleg1_2437 recombinant plasmid. …”
Get full text

Biolistic Transformation of Selected Orchid Hybrids for Improved Shelf Life and Cloning of Partical ACC Oxidase Gene from Oncidium Gower Ramsey by Mohana Anita

“…The Reverse- Transcription Polymerase Chain Reaction (RT-PCR) technique was used to isolate ACC oxidase gene fragments from the RNA samples and was successfully amplified from three stages (unpollinated, 18 hours and 48 hours after pollination). …”
Get full text

A potential dissemination of distinct MRSEs in a student population at a public university by Pung, Hui Ping, Amin Nordin, Syafinaz, Tengku Jamaluddin, Tengku Zetty Maztura, Mohd Desa, Mohd Nasir

“…In this current study, 75 CoNS isolates (S. epidermidis : n = 72; S. haemolyticus : n = 3) that were resistant to at least one antibiotic including one heterogeneous MRSE (carried mecA gene but was susceptible to all tested antibiotics) were further subjected to genomic DNA analysis by Random Amplified Polymorphic DNA-PCR (RAPD-PCR) approach. …”
Get full text

Development of a recombinant (rOMP36) vaccine from Pasteurella multocida A:1 against pasteurellosis in chickens and ducks by Handijatno, Didik

“…Therefore, the gene encoding 36 kDa OMP of P. multocida serotype A:1 was amplified by PCR before it was cloned in pET32 KL/LIC vector. …”
Get full text

Characterization of selected aquilaria species through DNA barcodes and identification of wound-response proteins of aquilaria malaccensis Lam. by Lee, Shiou Yih

“…For the identification of agarwood species of origin, a reference DNA barcode library was developed using eight candidate barcode loci (matK, rbcL, rpoB, rpoC1, psbA-trnH, trnL-trnF, ITS, and ITS2) amplified from 24 leaf accessions of seven Aquilaria species obtained from living trees. …”
Get full text

Development of a rapid diagnostic technique for equine influenza by D. Abdul Hadi, Abdul Rahman

“…The failure to isolate the circulating viral antigen was possibly due to horses that were not in the acute phase of the disease during the period when samples were collected, thus they did not shed a live virus, and the samples were collected from a situation of no form of outbreaks.With respect to development, of a rapid and reliable diagnostic technique, the NS1 gene of H3N8 subtype was amplified by RT-PCR and expressed in prokaryotic expression plasmid pRSET B in E. coli strain BL21 (DE3)plysS after induction with IPTG. …”
Get full text

Molecular characterization of Corynebacterium pseudotuberculosis and development of recombinant vaccine against caseous lymphadenitis by Shahridon, Syafiqah Adilah

“…Therefore, genes encoding the 31 kDa and 40 kDa surface proteins (SP) of C. pseudotuberculosis were amplified by polymerase chain reaction (PCR) before being cloned in pET32 Ek/LIC vector. …”
Get full text

Socio-demographic predictors of knowledge, attitude and practice in relation to Ebola Virus disease among medical and nursing students in a Teaching Hospital by Balami, Lawan Gana

“…There is neither a cure nor vaccine available and poor Knowledge, Attitude and Practices (KAP) among Health Care Workers (HCW) has amplified the spread of the disease. Aims and objectives: The aim of this study was to determine the predictors of KAP regarding the EVD among medical and nursing students in their clinical years of training in University of Maiduguri Teaching Hospital (UMTH) Nigeria. …”
Get full text

A broadband cooperative relay-based system for power line communication by Aiyelabowo, O. P.

“…A customized cooperative relaying system, implementing amplify-and-forward (AF) and decode-and-forward (DF), with placement of the relay modem at three different locations along the network in the PLC was developed. …”
Get full text

Over-expression of FabA, FabB, and FabF from Photobacterium marinum J15 for enhancement of omega-7 monounsaturated fatty acid production in Escherichia coli by Moi, Ibrahim Musa

“…Three fatty acids genes designated as fabA, fabB, and fabF were amplified from P. marinum J15 genome. The genes were cloned and over-expressed individually and in combinatorial in E. coli BL21(DE3) to investigate their effects on the omega-7 fatty acids content. …”
Get full text

Metabolic engineering of Escherichia coli carrying catechins biosynthesis genes of Camellia sinensis (L) kuntze by Umar, Kabir Mustapha

“…Three genes [CsF3H (l107bp), CsDFR (l044bp) and CsLCR (l044bp)] were amplified and cloned into pET expression vectors and transformed into E. coli BL21 (DE3) competent cells. …”
Get full text

Molecular analysis of the promoter and the core promoter region Of the smn2 gene in circumscribing the clinical severity of sma by Baig, Atif Amin

“…These five regions were PCR amplified and were cloned into pTOPO-2.1 e cloning vector. …”
Get full text

Design and development of new zinc porphyrin molecules as high performing photoacoustic contrast agents by Yasin, Amina

“…It was observed that less resonating functional groups in a phenyl group attached to the porphyrin core amplify the oscillation strengths of the alkoxy groups – a systematic variation in the absorption coefficients of the C-H vibrational modes was observed with relative change in the resonance of the molecular groups anchored. …”
Get full text

Development of a new robust hybrid automata algorithm based on surface electromyography (SEMG) signal for instrumented wheelchair control by Mohd Hanafi, Muhammad Sidik

“…These electrical changes that is called Motor Unit Action Potential (MUAP) can be acquired and amplified by electrodes located on targeted muscles changes can be recorded and analysed using sEMG devices. …”
Get full text