Near infrared light-mediated upconversion nanocrystals for precisely theranostic applications by Ai, Xiangzhao

“…Such enzyme-triggered cross-linking of UCNs leads to enhanced upconversion emission upon 808 nm laser irradiation, and in turn amplifies the singlet oxygen generation from the photosensitizers attached on UCNs. …”
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Mitochondrial DNA quantification as a tool for embryo viability assessment: retrospective analysis of data from single euploid blastocyst transfers by Ravichandran, K, McCaffrey, C, Grifo, J, Morales, A, Perloe, M, Munne, S, Wells, D, Fragouli, E

“…</p> <h4>Participants/Materials, Setting, Methods</h4> <p>DNA from blastocyst biopsy samples was amplified and then subjected to aneuploidy analysis using next generation sequencing or array comparative genomic hybridization. …”

The broad bipolar phenotype: sampling the experience of mood, stress and mental imagery by Malik, A

“…Holmes, Geddes, Colom and Goodwin (2008) hypothesised that mental imagery in BD may amplify emotion and worsen day to day mood extremes. …”

Epidemiological, social, diagnostic and economic evaluation of population screening for genital chlamydial infection. by Low, N, McCarthy, A, Macleod, J, Salisbury, C, Campbell, R, Roberts, T, Horner, P, Skidmore, S, Sterne, J, Sanford, E, Ibrahim, F, Holloway, A, Patel, R, Barton, P, Robinson, S, Mills, N, Graham, A, Herring, A, Caul, E, Davey Smith, G, Hobbs, F, Ross, J, Egger, M

“…Specimens were used in laboratory evaluations of an amplified enzyme immunoassay (PCE EIA) and two nucleic acid amplification tests [Cobas polymerase chain reaction (PCR), Becton Dickinson strand displacement amplification (SDA)]. …”

Mechanical modelling and imaging of human tendons by Scott, I

“…Hence, we also investigate how localised reductions in permeability and stiffness alter the local mechanical environment, to identify whether changes in collagen and proteoglycan content amplify or reduce the mechanical stimuli that occur with high- and low-frequency loading. …”

Effect of transpiration cooling on boundary layer transition for hypersonic flight by Kerth, P

“…At this freestream condition, strongly amplified second mode instabilities are present near the frustum injector segment. …”

Epidemiological, social, diagnostic and economic evaluation of population screening for genital chlamydial infection. by Low, N, McCarthy, A, Macleod, J, Salisbury, C, Campbell, R, Roberts, T, Horner, P, Skidmore, S, Sterne, J, Sanford, E, Ibrahim, F, Holloway, A, Patel, R, Barton, P, Robinson, S, Mills, N, Graham, A, Herring, A, Caul, E, Davey Smith, G, Hobbs, F, Ross, J, Egger, M

“…Specimens were used in laboratory evaluations of an amplified enzyme immunoassay (PCE EIA) and two nucleic acid amplification tests [Cobas polymerase chain reaction (PCR), Becton Dickinson strand displacement amplification (SDA)]. …”

Characterization of structure and function of small heat shock like proteins from psychrophilic yeast, glaciozyma antarctica pi12 in response to thermal stress by Farhan Nazaie Nasib

“…Out of the four sHSP genes identified in G. antarctica, two namely GasHSP07-010 and GasHSP12-338, were amplified and cloned using E. coli BL21(DE3). The proteins encoded by these genes were expressed at 37°C overnight and subsequently purified using immobilized metal chelate affinity chromatography (IMAC). …”
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First report of Burkholderia gladioli causing bacterial panicle blight of rice in Malaysia by Md Zali, Adam Zafdri, Ja'afar, Yasmin, Paramisparan, Karthik, Ismail, Siti Izera, Saad, Norsazilawati, Mohd Hata, Erneeza, Md Hatta, Muhammad Asyraf, Ismail, Mohd Razi, Yusof, Mohd Termizi, Zulperi, Dzarifah

“…To verify the identification of these isolates, the 16S rDNA gene was amplified using 16SF/16SR primers (Ramachandran et al. 2021), producing ~1,400 bp amplicons. …”

Pharmacological and toxicological effects of 6-hydroxy-2-mercaptopurine and 6-thioguanine in in vitro and in vivo models of arthritis by Nordin, Nurul Syuhada

“…The abnormal propagation and activation in many types of immune cells lead to the secretion of the most important pro-inflammatory cytokines mediators such as TNF-α, IL-6, and IL-1 that amplify the inflammation. Current treatment includes non-steroidal anti-inflammatory drugs (NSAIDs), glucocorticoids, disease-modifying anti-rheumatic drugs (DMARDs) and biological agents. …”
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Gene expression, characterization and in silico studies of delta fatty acid desaturases by Abd Halim, Nur Farah Anis

“…Δ6 FAD was isolated, and PCR amplified from Anoxybacillus geothermalis, cloned in pGEX-4T1 vector and expressed in E. coli BL21 (DE3) with the expected amplicon of 1104 bp encoding for 368 amino acids obtained with the restriction enzymes BamHI and EcoRI. …”
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Characteristics, genetic diversity and pathogenic variability of Fusarium oxysporum f. sp. niveum in watermelon (Citrullus lanatus var. lanatus (Thunb.) Matsum. & Nakai) by Rahman, Muhammad Ziaur

“…The Fon forma speciales-specific primers (Fn-1/Fn-2 and Fon-1/Fon-2) were amplified amplicons of 320 bp and 174 bp in 100% and 91% of the isolates, respectively. …”
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Prevalence of West Nile virus in domesticated mammals (cattle, goat, horse and pig) in selected areas of Peninsular Malaysia by Mohammed, Mohammed Nma

“…In nature, the virus is maintained in a cycle between wild birds that served as amplifiers and Culex mosquitos which are the vectors. …”
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Development of DNA Based Technologies for Detection of Genetically Modified Organisms by Cheah, Yoke Kqueen

“…Although this nonradioactive DNA probes is lower sensitivity than PCR, it is easier to process for large numbers of samples at once and inexpensive. Random amplified polymorphic DNA (RAPD) analysis used in this study was able to distinguish between commercially obtained genetically modified materials with normal food. …”
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Significance of L2 Lipase N-Terminal Residue in Maintaining Protein Integrity by Md. Jelas, Nur Hana

“…The purified genomic DNA was used as the template to amplify L2 lipase gene. Two degenerate primers, FT and RT were designed for the gene amplification. …”
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Genetic Characterization of South African and Australian Purebred Boer Goats and Australian Crosses Using Microsatellites by Ali Kamarulzaman, Hamidah

“…Only 46 of the microsatellite loci were successfully amplified, of which 15 were monomorphic and 31 were polymorphic. …”
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Micropropagation of tea (Camellia sinensis (L.) kuntze) using temporary immersion system by Gonbad, Reza Azadi

“…Finally the genetic fidelity of the micropropaged tea plants wasssessed using ISSR (Inter-Simple Sequence Repeats). The amplified ISSR locus from 11 markers showed 100% similarity between micropropagated plants (derived in the third subculture of by TIS) and 10 randomly selected acclimatized plantlets with their mother plant DNA of tea clone Iran 100. …”
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Genetic studies on physiological traits in wheat (Triticum aestivum L.) under normal and water stress conditions by Naroui Rad, Mohammad Reza

“…A total of 41 SSR primer pairs were used to screen the parents; five polymorphic primer pairs were identified which showed polymorphisms between parents, and three of them strongly amplified polymorphic bands among the F2 population. …”
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Mitochondrial DNA polymorphism in seven cattle breeds in Malaysia by Yow, Weng Kit

“…The D-loop, cyt b and two ND5 regions were amplified using specific primers. These regions were digested with nine, five and two restriction endonucleases (RE),respectively, generating 16 polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) loci. …”
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Creation of potential gene cassette for recombinant protein expression in green microalgal system by Kadkhodaei, Saeid

“…To develop a proprietary series of microalgae specific expression vectors, nine fragments containing the Gateway recombination cassette, optimized genes of interest (2), promoters (2), 5’- UTR, interon, 3’-UTR (2), MARs (2) and pUC18 backbone were amplified using the most high fidelity enzyme available (KAPA). …”
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