Epidemiology of Trypanosoma evansi infection in horses in Peninsular Malaysia and the variant surface glycoprotein of the isolates by Elshafie, Elshafie Ibrahim

“…Morphometric study revealed the mean total length of the trypanosomes including the free flagellum was 27.94 ± 2.63 μm. PCR successfully amplified a trypanosome specific 257 bp in 1.14% of samples (6/527, CI: 2.4-0.52%) and was confirmed by nucleotide sequences. …”
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Biochemical and molecular characterisation of selected microorganisms isolated from beef, chicken, mutton and pork meat products by Amie, Ceesay

“…Thirty four E. coli, was examined by molecular markers such as BOXAIR, Enterobacterial repetitive intergenic consensus (ERIC), polytrinucleotide (GTG)5 and random amplified polymorphic DNA polymerase chain reaction ((RAPD-PCR) to generate genetic fingerprints, while 56 Lactobacillus and 42 Pseudomonas species were typed using RAPD and (GTG)-PCR to generate fingerprint data. …”
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Phenotypic and molecular analyses of coagulase negative Staphylococci, predominantly comprising Staphylococcus epidermidis, isolated from a student population in Malaysia by Pung, Hui Ping

“…CoNS isolates that were resistant to at least one antibiotic or were mecA positive were subjected to species identification based on tuf gene sequencing, screening of erythromycin resistance associated genes (ermA, ermB, ermC, and msrA), random amplified polymorphic DNA- (RAPD) and enterobacterial repetitive intergenic consensuspolymerase chain reaction (ERIC-PCR). …”
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Development of reverse dot blot hybridisation strip assays to identify common beta-thalassaemia alleles in Malays and Chinese in Malaysia by Teh, Lai Kuan

“…ARMS-PCR was used to confirm and validate the presence of the six mutations used in the RDBH-Strip Assays. It amplified each mutation as a separate and distinct PCR product. …”
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Detection of chromosomal translocation [T(12;21)] and regulatory T cells in acute leukaemias using flow cytometry by Idris, Siti Zuleha

“…In-situ reverse transcriptase-polymerase chain reaction (RT-PCR) is a technique where cDNA is amplified within the cell. By combining these two methods and use of fluorescent primers, it is possible to detect chromosomal translocations within single cells using flow cytometry. …”
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First report of Bipolaris cactivora causing brown leaf spot in rice in Malaysia by M. Z., Nur Ain Izzati, M. Z. A., Madihah, K., Nor Azizah, A., Najihah, M., Muskhazli

“…Primers ITS1/ITS4 and GPDH-F/R were used to amplify and subsequently sequence the internal transcribed spacer (ITS1-5.8S-ITS2) region (White et al. 1990) and glycerol-3-phosphate dehydrogenase (gpd) gene, respectively. …”

First report of leaf spot on oil palm caused by Phyllosticta capitalensis in Malaysia by Nasehi, A., Sathyapriya, H., Wong, M. Y.

“…For fungal identification to species level, the internal transcribed spacer region (ITS) of the representative isolate UPM-Ph1 was amplified utilizing the universal primers ITS5/ITS4 (White et al. 1990) and then sequenced. …”

Association of single nucleotide polymorphisms of SPAG11 gene with semen quality and testicular traits of boer goats by Harighi, Mohammadfazel

“…Primers were designed to amplify two exons of the given gene. The SNPs were detected using single-base extension (SBE) method along with molecular cloning technique. …”
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First report of Colletotrichum siamense causing blossom blight on Thai basil (Ocimum basilicum L.) in Malaysia by Ismail, Siti Izera, Rahim, Nur Adlina, Zulperi, Dzarifah

“…Internal transcribed spacer (ITS) region, calmodulin (CAL), actin (ACT), and chitin synthase-1 (CHS-1) were amplified using ITS5/ITS4 (White et al. 1990), CL1C/CL2C (Weir et al. 2012), ACT-512F/783R, and CHS-79F/CHS-345R primer sets (Carbone and Kohn 1999), respectively. …”
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First Report of Fusarium incarnatum-equiseti species complex causing leaf spot on rockmelon (Cucumis melo L.) in Malaysia by Ismail, Siti Izera, Noor Asha, Nur Ainina, Zulperi, Dzarifah

“…To confirm the identity of the fungus, two sets of primers, ITS4/ITS5 (White et al. 1990) and EF1-728F/EF1-986R (Carbone and Kohn 1999), were used to amplify complete internal transcribed spacer (ITS) and partial translation elongation factor 1-alpha (TEF1-α) genes, respectively. …”
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First report of Neoscytalidium dimidiatum causing fruit rot on Guava (Psidium guajava L.) in Malaysia by Ismail, Siti Izera, Ahmad Dahlan, Khoirunnisa, Abdullah, Sumaiyah, Zulperi, Dzarifah

“…The internal transcribed spacer (ITS) region of rDNA and translation elongation factor 1-alpha (TEF1-α) gene were amplified using ITS5/ITS4 (White et al. 1990) and EF1-728F/EF1-986R primer sets (Carbone and Kohn 1999), respectively. …”
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First report of Nigrospora sphaerica causing leaf spot on watermelon (Citrullus lanatus L.) in Malaysia by Ismail, Siti Izera, Abd Razak, Nur Fatahiah

“…The internal transcribed spacer (ITS) region of rDNA and the translation elongation factor 1-alpha (TEF1-α) gene were amplified using the ITS5/ITS4 (White et al. 1990) and EF1-728F/EF1-986R primer sets (Carbone and Kohn 1999), respectively. …”
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Systematic studies of orchid genus Coelogyne in Peninsular Malaysia by Yoh, Kok Hon

“…Whereas SCAR marker pair CST_f / CST_r amplified a single band of 854 bp in C. stenochila and two bands of different sizes (372 bp and 858 bp) for C. tiomanensis but no amplification in C. kaliana. …”
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Genetic Studies and Growth Performance of the Yellow Catfish, Mystus Nemurus (Cuv. & Val.) in Thailand by Leesanga, Sanga

“…Five arbitrary primers were chosen to amplify products which showed 28 polymorphic loci (60.87 %). …”
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Development of a single-chain fragment variable fused-mutant HALT-1 recombinant immunotoxin against G12V mutated KRAS colorectal cancer cells by Teo, Michelle Yee Mun, Ng, Jeremy Jeack Ceen, Jung, Yin Fong, Jung, Shan Hwang, Song, Adelene Ai Lian, Lim, Renee Lay Hong, In, Lionel Lian Aun

“…Results: The VH and VL genes from spleen RNA of mice immunized with 164-D and 68-V were amplified and randomly linked together, using SOE-PCR producing band sizes about 750 bp. …”
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Improved Image Enhancement Method For Non-Uniform Illumination And Low Ontrast Images Using Bihistogram Modification Approach by Kong , Teck Long

“…Qualitative analysis shows that the proposed BHMIC has good performance in detail preservation, contrast enhancement and illumination condition enhancement without significantly amplifying unwanted noise. The quantitative analysis shows that the proposed BHMIC is 38% to 42.9%, 0.8% to 4.7% and 0.7% to 2.3% better than other tested methods in EME, NIQE and entropy, respectively. …”
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Analysis of Leptin receptor, Glucocorticoid receptor,B-3 Aderenergic receptor and Tumour Necrosis Factor-a Gene Polymorphism in Type 2 Diabetes Mellitus in Kelantan Malays. by Isa, Nizam, Ismail, Abd. Aziz AI-Safi, Mohamed, Mafauzy

“…The genomic DNA were amplified using specific primers and polymerase chain reaction (PCR) conditions for each specific gene of interest according to published works with modification. …”
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Diagnosis of nasopharyngeal carcinoma by DNA amplification of epstein-bar virus genomes in tissue obtained by biopsy and fine-needle aspiration by Hassan, Shahid, Yap, Yap, Ravichandran, M., Chan, Melissa Li-Ann

“…Tissue from 70 fine-needle aspirations were similarly obtained. 35 belonged to NPC-positive patients, and 35 of other pathologies served as controls. DNA was extracted, amplified with forward and reverse primers for EBNA 1, EBNA2, LMP1 genes and human a-actin gene as control, and detected by electrophoresis. …”
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Geenetic relationship among the negrito (Semang) tribes of peninsular Malaysia: the microsatellite approach by Peng, Hoh Boon

“…Optimizations were done and 7 of the primers were successful. These markers were amplified on the samples and the fluorescent-labeled fragments were electrophoresed through ABB I 00 and analyzed using GeneScan analysis software. …”
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Modelling of advanced submicron gate InGaAs/InAIAs pHEMTS and RTD devices for very high frequency applications by Mat Jubadi, Warsuzarina

“…These disadvantages degrade device performance, especially in Monolithic Microwave Integrated Circuit (MMIC) low noise amplifiers (LNAs). The optimisation of InAlAs/InGaAs epilayer structures through advanced bandgap engineering together with gate length reduction from 1 m into deep sub-μm regime is the key solution to enabled high breakdown and ultra-high speed, low noise pHEMT devices to be fabricated. …”
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