LLIN evaluation in Uganda project (LLINEUP): The fabric integrity, chemical content and bioefficacy of long-lasting insecticidal nets treated with and without piperonyl butoxide ac... by Frank Mechan, Agaba Katureebe, Violet Tuhaise, Martin Mugote, Ambrose Oruni, Ismail Onyige, Kawesa Bumali, Jonathan Thornton, Kilama Maxwell, Mary Kyohere, Moses R. Kamya, Peter Mutungi, Simon P. Kigozi, Adoke Yeka, Jimmy Opigo, Catherine Maiteki-Sebuguzi, Samuel Gonahasa, Janet Hemingway, Grant Dorsey, Lisa J. Reimer, Sarah G. Staedke, Martin J. Donnelly, Amy Lynd

“…Bioefficacy was assessed with three-minute World Health Organisation (WHO) Cone and Wireball assays using pyrethroid-resistant Anopheles gambiae, with 1-h knockdown and 24-h mortality recorded. …”
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Development of CRISPR/Cas9-Mediated Gene-Drive Construct Targeting the Phenotypic Gene in Plutella xylostella by Muhammad Asad, Muhammad Asad, Muhammad Asad, Muhammad Asad, Dan Liu, Dan Liu, Dan Liu, Dan Liu, Jianwen Li, Jianwen Li, Jianwen Li, Jianwen Li, Jing Chen, Jing Chen, Jing Chen, Jing Chen, Guang Yang, Guang Yang, Guang Yang, Guang Yang, Guang Yang

“…The clustered regularly interspersed palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) mediated gene-drive system has been demonstrated in dipteran insect species, including Drosophila and Anopheles, not yet in other insect species. Here, we have developed a single CRISPR/Cas9-mediated gene-drive construct for Plutella xylostella, a highly-destructive lepidopteran pest of cruciferous crops. …”
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In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius p... by Fabio Faria da Mota, Fabio Faria da Mota, Daniele Pereira Castro, Daniele Pereira Castro, Cecilia Stahl Vieira, Marcia Gumiel, Julia Peixoto de Albuquerque, Nicolas Carels, Patricia Azambuja, Patricia Azambuja

“…S. marcescens have been recently found in the gut of hematophagous insects that act as parasite vectors, such as Anopheles, Rhodnius, and Triatoma. While some S. marcescens strains have been reported as symbiotic or pathogenic to other insects, the role of S. marcescens populations from the gut microbiota of Rhodnius prolixus, a vector of Chagas’ disease, remains unknown. …”
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Pigsties near dwellings as a potential risk factor for the prevalence of Japanese encephalitis virus in adult in Shanxi, China by Xiaojie Ren, Shihong Fu, Peifang Dai, Huanyu Wang, Yuanyuan Li, Xiaolong Li, Wenwen Lei, Xiaoyan Gao, Ying He, Zhi Lv, Jingxia Cheng, Guiqin Wang, Guodong Liang

“…Results A total of 7 943 mosquitoes belonging to six species in four genera were collected; Culex tritaeniorhynchus accounted for 73.08% (5 805/7 943), C. pipiens pallens for 24.75% (1 966/7 943), and the remaining 3% (104/ 7943) consisted of Anopheles sinensis, Aedes vexans, Ae. dorsalis, and Armigeres subalbatus. …”
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Dynamics of asymptomatic Plasmodium falciparum and Plasmodium vivax infections and their infectiousness to mosquitoes in a low transmission setting of Ethiopia: a longitudinal obse... by Elifaged Hailemeskel, Surafel K. Tebeje, Jordache Ramjith, Temesgen Ashine, Kjerstin Lanke, Sinknesh W. Behaksra, Tadele Emiru, Tizita Tsegaye, Abrham Gashaw, Soria Kedir, Wakweya Chali, Endashaw Esayas, Temesgen Tafesse, Haile Abera, Mikiyas Gebremichael Bulto, Girma Shumie, Beyene Petros, Hassen Mamo, Chris Drakeley, Endalamaw Gadisa, Teun Bousema, Fitsum G. Tadesse

“…Method: Total parasite and gametocyte kinetics were determined by molecular methods; infectivity to Anopheles arabiensis mosquitoes by repeated membrane feeding assays. …”
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Beauveria bassiana sebagai Alternatif Hayati dalam Pengendalian Nyamuk by Bina Ikawati

“…<span> </span> This<span> </span> fungus<span> </span> is<span> </span> proven<span> </span> effective<span> </span> in<span> </span> killing<span> </span>genus<span> </span> <em>Culex, Aedes, Anopheles</em><span> </span> in<span> </span> the<span> </span> laboratory<span> </span> research.…”
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Ecologia de mosquitos (Diptera: Culicidae) em áreas do Parque Nacional da Serra da Bocaina, Brasil.I - Distribuição por habitat Ecology of mosquitoes (Diptera: Culicidae) in areas... by Anthony Érico Guimarães, Carla Gentile, Catarina M Lopes, Alessandro Sant'Anna, Alexandre M Jovita

“…Runchomyia reversa and Anopheles cruzii were the most abundant, reaching 52.5% and 17.9% of the total collected specimens, respectively. …”
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Effectiveness of piperonyl butoxide and pyrethroid-treated long-lasting insecticidal nets (LLINs) versus pyrethroid-only LLINs with and without indoor residual spray against malari... by Natacha Protopopoff, Jacklin F. Mosha, Louisa A. Messenger, Eliud Lukole, Jacques D. Charlwood, Alexandra Wright, Enock Kessy, Alphaxard Manjurano, Franklin W. Mosha, Immo Kleinschmidt, Mark Rowland

“…After 33 months the effect was weaker in the ITT analysis (prevalence 59.6% versus 49.9%, OR: 0.60, 95%CI:0.32–1.13, p-value: 0.1131) but still evident in the PP analysis (57.2% versus 44.2%, OR: 0.34, 95%CI: 0.16–0.71, p-value: 0.0051). Mean number of Anopheles per night collected per house was similar between PBO-Pyrethroid-LLIN groups (5.48) and std-LLIN groups (5.24) during the third year. …”
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A Simple CO₂ Generating System Incorporated with CDC Light Trap for Sampling Mosquito Vectors by Sutasinee Madang, Jassada Saingamsook, Atiporn Saeung, Pradya Somboon

“…A total of 1620 mosquitoes (97.7% being females) consisting of <i>Aedes</i>, <i>Anopheles</i>, <i>Armigeres</i>, <i>Coquilletidia</i>, <i>Culex</i> and <i>Mansonia</i> were captured across three different sampling sets from all villages. …”
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How house design affects malaria mosquito density, temperature, and relative humidity: an experimental study in rural Gambia by Ebrima Jatta, BSc, Musa Jawara, MSc, John Bradley, PhD, David Jeffries, PhD, Balla Kandeh, MSc, Jakob B Knudsen, MAA, Anne L Wilson, PhD, Margaret Pinder, PhD, Umberto D'Alessandro, ProfMD, Steve W Lindsay, ProfPhD

“…Primary measurements were mean number of Anopheles gambiae and mean temperature for each house typology. …”
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Advances and challenges in automated malaria diagnosis using digital microscopy imaging with artificial intelligence tools: A review by Carles Rubio Maturana, Carles Rubio Maturana, Allisson Dantas de Oliveira, Sergi Nadal, Besim Bilalli, Francesc Zarzuela Serrat, Mateu Espasa Soley, Mateu Espasa Soley, Elena Sulleiro Igual, Elena Sulleiro Igual, Elena Sulleiro Igual, Mercedes Bosch, Anna Veiga Lluch, Alberto Abelló, Daniel López-Codina, Tomàs Pumarola Suñé, Tomàs Pumarola Suñé, Elisa Sayrol Clols, Joan Joseph-Munné

“…It is transmitted to humans by the bite of an infected female Anopheles mosquito. It is the most common disease in resource-poor settings, with 241 million malaria cases reported in 2020 according to the World Health Organization. …”
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An Integrated Molecular Approach to Untangling Host–Vector–Pathogen Interactions in Mosquitoes (Diptera: Culicidae) From Sylvan Communities in Mexico by Luis M. Hernández-Triana, Javier A. Garza-Hernández, Aldo I. Ortega Morales, Sean W. J. Prosser, Paul D. N. Hebert, Nadya I. Nikolova, Elsa Barrero, Erick de J. de Luna-Santillana, Vicente H. González-Alvarez, Ramón Mendez-López, Rahuel J. Chan-Chable, Anthony R. Fooks, Mario A. Rodríguez-Pérez

“…High intraspecific divergence values (&gt;2%) suggesting cryptic species complexes were observed in nine taxa: Anopheles eiseni (5.39%), An. pseudopunctipennis (2.79%), Ae. podographicus (4.05%), Culex eastor (4.88%), Cx. erraticus (2.28%), Toxorhynchites haemorrhoidalis (4.30%), Tr. pallidiventer (4.95%), Wyeomyia adelpha/Wy. guatemala (7.30%), and Wy. pseudopecten (4.04%). …”
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Patterns of anopheline feeding/resting behaviour and Plasmodium infections in North Cameroon, 2011–2014: implications for malaria control by Wolfgang Eyisap Ekoko, Parfait Awono-Ambene, Jude Bigoga, Stanislas Mandeng, Michael Piameu, Narcisse Nvondo, Jean-Claude Toto, Philippe Nwane, Salomon Patchoke, Lili Ranaise Mbakop, Jerome Achille Binyang, Martin Donelly, Immo Kleinschmidt, Tessa Knox, Arthur Mbida Mbida, Alain Dongmo, Etienne Fondjo, Abraham Mnzava, Josiane Etang

“…Results Overall, 9376 anophelines belonging to 14 species/sibling species were recorded. Anopheles gambiae (s.l.) [An. arabiensis (73.3%), An. coluzzii (17.6%) and An. gambiae (s.s.) (9.1%)] was predominant (72%), followed by An. funestus (s.l.) (20.5%) and An. rufipes (6.5%). …”
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Fern-synthesized nanoparticles in the fight against malaria: LC/MS analysis of Pteridium aquilinum leaf extract and biosynthesis of silver nanoparticles with high mosquitocidal and... by Panneerselvam, Chellasamy, Murugan, Kadarkarai, Roni, Mathath, Aziz, Al Thabiani, Suresh, Udaiyan, Rajaganesh, Rajapandian, Madhiyazhagan, Pari, Subramaniam, Jayapal, Dinesh, Devakumar, Nicoletti, Marcello, Higuchi, Akon, Alarfaj, Abdullah A., Munusamy, Murugan A., Kumar, Suresh, Desneux, Nicolas, Benelli, Giovanni

“…In mosquitocidal assays, LC50 of P. aquilinum leaf extract against Anopheles stephensi larvae and pupae were 220.44 ppm (larva I), 254.12 ppm (II), 302.32 ppm (III), 395.12 ppm (IV), and 502.20 ppm (pupa). …”
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Fern-synthesized nanopeprintss in the fight against malaria: LC/MS analysis of Pteridium aquilinum leaf extract and biosynthesis of silver nanopeprintss with high mosquitocidal and... by Panneerselvam, Chellasamy, Murugan, Kadarkarai, Roni, Mathath, Aziz, Al Thabiani, Suresh, Udaiyan, Rajaganesh, Rajapandian, Madhiyazhagan, Pari, Subramaniam, Jayapal, Dinesh, Devakumar, Nicoletti, Marcello, Higuchi, Akon, Alarfaj, Abdullah A., Munusamy, Murugan A., Kumar, Suresh, Desneux, Nicolas, Benelli, Giovanni

“…In mosquitocidal assays, LC50 of P. aquilinum leaf extract against Anopheles stephensi larvae and pupae were 220.44 ppm (larva I), 254.12 ppm (II), 302.32 ppm (III), 395.12 ppm (IV), and 502.20 ppm (pupa). …”
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Community evaluation of VECTRON™ T500, a broflanilide insecticide, for indoor residual spraying for malaria vector control in central Benin; a two arm non-inferiority cluster rando... by Corine Ngufor, Renaud Govoetchan, Augustin Fongnikin, Corneille Hueha, Juniace Ahoga, Thomas Syme, Abel Agbevo, Abdoulaye Daleb, Graham Small, Derric Nimmo, John Bradley, Rock Aikpon, Laurent Iyikirenga, Razaki Osse, Filemon Tokponnon, Germain Gil Padonou

“…We performed a two-arm non-inferiority community randomised evaluation of VECTRON™ T500, compared to Fludora® Fusion against pyrethroid-resistant Anopheles gambiae s.l. in an area of high coverage with pyrethroid-only nets in the Za-Kpota District of central Benin. …”
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Update and elucidation of Plasmodium kinomes: Prioritization of kinases as potential drug targets for malaria by Joyce Villa Verde Bastos Borba, Arthur de Carvalho e Silva, Marília Nunes do Nascimento, Letícia Tiburcio Ferreira, Aline Rimoldi, Luísa Starling, Pablo Ivan Pereira Ramos, Fabio Trindade Maranhão Costa, Carolina Horta Andrade

“…Malaria is a tropical disease caused by Plasmodium spp. and transmitted by the bite of infected Anopheles mosquitoes. Protein kinases (PKs) play key roles in the life cycle of the etiological agent of malaria, turning these proteins attractive targets for antimalarial drug discovery campaigns. …”
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Blood-stage antiplasmodial activity and oocyst formation-blockage of metallo copper-cinchonine complex by Camila Martins Gomes Morais, Camila Martins Gomes Morais, Ramayana Morais de Medeiros Brito, Ramayana Morais de Medeiros Brito, Aleksandra Weselucha-Birczyńska, Valeska Santana de Sena Pereira, Valeska Santana de Sena Pereira, Jordam William Pereira-Silva, Jordam William Pereira-Silva, Alexandre Menezes, Felipe Arley Costa Pessoa, Martyna Kucharska, Malwina Birczyńska-Zych, Malwina Birczyńska-Zych, Claudia María Ríos-Velásquez, Valter Ferreira de Andrade-Neto

“…The oocyst formation-blocking assay was performed by experimental infection of Anopheles aquasalis with P. vivax infected blood, which was treated with different concentrations of CinCu, cinchonine, and primaquine. …”
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Entomological Investigation and Detection of Dengue Virus Type 1 in Aedes (Stegomyia) albopictus (Skuse) During the 2018–2020 Outbreak in Zhejiang Province, China by Qinmei Liu, Qinmei Liu, Jinna Wang, Juan Hou, Yuyan Wu, Hengduan Zhang, Dan Xing, Jian Gao, Chunxiao Li, Xiaoxia Guo, Yuting Jiang, Zhenyu Gong, Tongyan Zhao

“…The main species were Culex pipiens quinquefasciatus/pallens (41.32%), Culex tritaeniorhynchus (47.6%), Aedes albopictus (2.5%), Anopheles sinensis (5.87%), Armigeres subalbatus (2.64%) and other mosquito species (0.07%). …”
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First Report of Rubber Collection Bowls & Plastic and Bamboo Water Containers as the Major Breeding Source of <i>Ae. albopictus</i> with the Indigenous Transmission of Dengue and C... by Saurav Biswas, Jadab Rajkonwar, Tulika Nirmolia, Sasmita Rani Jena, Ujjal Sarkar, Dibya Ranjan Bhattacharyya, Biswajyoti Borkakoty, Apoorva Pandey, Sarala K. Subbarao, Tapan Majumder, Rocky Pebam, Phiroz Gogoi, Kongkon Mahanta, Kanwar Narain, Ipsita Pal Bhowmick

“…Additionally, rubber collection bowls, ponds, and water containers also showed the presence of <i>Culex quinquefasciatus</i> and <i>Culex vishnui</i>, known JE vectors from this area, and reported JE cases as well. Different <i>Anopheles</i> vector spp. from this known malaria-endemic area were also found, corroborating this area as a hotbed of several vectors and vector-borne diseases. …”
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