RNA and Protein Determinants Mediate Differential Binding of miRNAs by a Viral Suppressor of RNA Silencing Thus Modulating Antiviral Immune Responses in Plants by Robert Pertermann, Ralph Peter Golbik, Selvaraj Tamilarasan, Torsten Gursinsky, Selma Gago-Zachert, Vitantonio Pantaleo, Iris Thondorf, Sven-Erik Behrens

“…Addressing this, we analyzed the binding of p19 to the miRNAs 162 and 168, which regulate the expression of the important RNA silencing constituents Dicer-like 1 (DCL1) and Argonaute 1 (AGO1), respectively. p19 binds miRNA162 with similar high affinity as siRNA, whereas the affinity for miRNA168 is significantly lower. …”
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Imaging-Based Resistance Assay Using Enhanced Luminescence-Tagged Pseudomonas syringae Reveals a Complex Epigenetic Network in Plant Defense Signaling Pathways by Dinesh S. Pujara, Sung-Il Kim, Ji Chul Nam, José Mayorga, Isabella Elmore, Manish Kumar, Hisashi Koiwa, Hong-Gu Kang

“…Compromises in DNA methylation in the CG context led to enhanced resistance against virulent Pseudomonas syringae pv. tomato. Dicer-like and Argonaute proteins, important in the biogenesis and exerting the effector function of small RNAs, respectively, showed modest but distinct requirements for effector-triggered immunity and basal resistance to P. syringae pv. tomato. …”
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Catalytic properties and biological function of a PIWI-RE nuclease from Pseudomonas stutzeri by Fei Huang, Xiaoyi Xu, Huarong Dong, Nuolan Li, Bozitao Zhong, Hui Lu, Qian Liu, Yan Feng

“…Abstract Background Prokaryotic Argonaute (pAgo) proteins are well-known oligonucleotide-directed endonucleases, which contain a conserved PIWI domain required for endonuclease activity. …”
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Bioinformatics and in vitro study reveal the roles of microRNA-346 in high glucose-induced human retinal pigment epithelial cell damage by Peng Li, Li Wang, Qing Liu, Zhao-Jiang Du

“…HG inhibited cell proliferation and induced apoptosis, which were partly reversed by transfecting an miR-346 inhibitor, which even decreased the ROS levels elevated due to HG. Argonaute 2 (AGO2) was a target of miR-346. CONCLUSION: miR-346 is a key miRNA and plays an important role in HG-induced damage in human retinal pigment epithelial cells.…”
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2′-O-Methyl at 20-mer Guide Strand 3′ Termini May Negatively Affect Target Silencing Activity of Fully Chemically Modified siRNA by Sarah M. Davis, Jacquelyn Sousa, Lorenc Vangjeli, Matthew R. Hassler, Dimas Echeverria, Emily Knox, Anton A. Turanov, Julia F. Alterman, Anastasia Khvorova

“…Molecular modeling analysis suggests that 2′-O-methyl modification may impair guide strand interactions within the PAZ domain of argonaute-2, which may affect target recognition and cleavage, specifically when guide strands are 20-nt long. …”
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p63, a key regulator of Ago2, links to the microRNA-144 cluster by Benfan Wang, H. Helena Wu, Yasser Abuetabh, Sarah Leng, Sandra T. Davidge, Elsa R. Flores, David D. Eisenstat, Roger Leng

“…Abstract As a key component of the RNA-induced silencing complex (RISC), Argonaute2 (Ago2) exhibits a dual function regulatory role in tumor progression. …”
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Long non-coding RNA LINC00565 regulates ADAM19 expression through sponging microRNA-532-3p, thereby facilitating clear cell renal cell carcinoma progression by Bin Meng, Pengfei Wang, Chaofei Zhao, Guangwei Yin, Xin Meng, Lin Li, Shengyong Cai, Chengquan Yan

“…TCGA database, dual-luciferase report detection, and Argonaute 2 RNA immunoprecipitation were utilized to confirm the relationships between LINC00565 and miR-532-3p and between miR-532-3p and ADAM19, respectively. …”
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Critical Roles of PIWIL1 in Human Tumors: Expression, Functions, Mechanisms, and Potential Clinical Implications by Peixin Dong, Ying Xiong, Yosuke Konno, Kei Ihira, Daozhi Xu, Noriko Kobayashi, Junming Yue, Junming Yue, Hidemichi Watari

“…PiRNAs are thought to bind to PIWI proteins (PIWL1-4, a subfamily of Argonaute proteins), forming piRNA/PIWI complexes that influence gene expression at the transcriptional or post-transcriptional levels. …”
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Alphavirus infection triggers antiviral RNAi immunity in mammals by Jing Kong, Yuanyuan Bie, Wenting Ji, Jiuyue Xu, Bao Lyu, Xiaobei Xiong, Yang Qiu, Xi Zhou

“…These SFV-vsiRNAs are located at a particular region within the 5′ terminus of the SFV genome, Argonaute loaded, and active in conferring effective anti-SFV activity. …”
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RNAi as a Tool to Study Virulence in the Pathogenic Yeast Candida glabrata by Olena P. Ishchuk, Olena P. Ishchuk, Khadija Mohamed Ahmad, Katarina Koruza, Klara Bojanovič, Marcel Sprenger, Lydia Kasper, Sascha Brunke, Bernhard Hube, Bernhard Hube, Torbjörn Säll, Thomas Hellmark, Birgitta Gullstrand, Christian Brion, Kelle Freel, Joseph Schacherer, Birgitte Regenberg, Wolfgang Knecht, Wolfgang Knecht, Jure Piškur

“…We established an RNA interference (RNAi) system in C. glabrata by expressing the Dicer and Argonaute genes from Saccharomyces castellii (a budding yeast with natural RNAi). …”
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A positive feedback loop of lncRNA-RMRP/ZNRF3 axis and Wnt/β-catenin signaling regulates the progression and temozolomide resistance in glioma by Tie Liu, Jie Hu, Bo Han, Shishan Tan, Wenqing Jia, Yu Xin

“…IGF2BP3 knockdown weakened the interaction of Argonaute 2 (Ago2) and ZNRF3. RMRP reduced ZNRF3 expression and mRNA stability by IGF2BP3. …”
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Different Genetic Sources Contribute to the Small RNA Population in the Arbuscular Mycorrhizal Fungus Gigaspora margarita by Alessandro Silvestri, Massimo Turina, Valentina Fiorilli, Laura Miozzi, Francesco Venice, Paola Bonfante, Luisa Lanfranco

“…Our findings show that G. margarita is equipped with a complete set of RNAi-related genes characterized by the expansion of the Argonaute-like (AGO-like) gene family that seems a common trait of AMF. …”
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The roles of rice microRNAs in rice-Magnaporthe oryzae interaction by Yan Li, John Martin Jerome Jeyakumar, Qin Feng, Zhi-Xue Zhao, Jing Fan, Muhammad Ibrahim Khaskheli, Wen-Ming Wang

“…The mature miRNAs are loaded onto ARGONAUTE (AGO) proteins, forming a miRNA-induced gene silencing complex (miRISC). …”
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henn-1/HEN1 Promotes Germline Immortality in Caenorhabditis elegans by Joshua M. Svendsen, Kailee J. Reed, Tarah Vijayasarathy, Brooke E. Montgomery, Rachel M. Tucci, Kristen C. Brown, Taylor N. Marks, Dieu An H. Nguyen, Carolyn M. Phillips, Taiowa A. Montgomery

“…Keywords: miRNA, piRNA, siRNA, Argonaute, C. elegans, hen1, piwi, methylation, transgenerational, RNAi…”
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Identification of microRNA-like RNAs in mycelial and yeast phases of the thermal dimorphic fungus Penicillium marneffei. by Susanna K P Lau, Wang-Ngai Chow, Annette Y P Wong, Julian M Y Yeung, Jessie Bao, Na Zhang, Si Lok, Patrick C Y Woo, Kwok-Yung Yuen

“…Two genes, dcl-1 and dcl-2, encoding putative Dicer-like proteins and the gene, qde-2, encoding Argonaute-like protein, were identified in P. marneffei. …”
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Silencing of HuR Inhibits Osteosarcoma Cell Epithelial-Mesenchymal Transition via AGO2 in Association With Long Non-Coding RNA XIST by Yongming Liu, Yuan Zhang, Jinxue Zhang, Jingchang Ma, Xuexue Xu, Yuling Wang, Ziqing Zhou, Dongxu Jiang, Shen Shen, Yong Ding, Yong Zhou, Ran Zhuang, Ran Zhuang

“…RNA immunoprecipitation was performed to determine the association of the long non-coding RNA (lncRNA) XIST and argonaute RISC catalytic component (AGO) 2 with HuR. …”
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Genome-Wide Identification and Expression Analyses of the Cotton <i>AGO</i> Genes and Their Potential Roles in Fiber Development and Stress Response by Mingchuan Fu, Yizhen Chen, Hao Li, Liguo Wang, Renzhong Liu, Zhanji Liu

“…Argonaute proteins (AGOs) are indispensable components of RNA silencing. …”
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vtRNA2-1/nc886 Produces a Small RNA That Contributes to Its Tumor Suppression Action through the microRNA Pathway in Prostate Cancer by Rafael Sebastián Fort, Beatriz Garat, José Roberto Sotelo-Silveira, María Ana Duhagon

“…Re-analysis of PAR-CLIP and knockout of microRNA biogenesis enzymes data showed that these small RNAs are products of DICER, independent of DROSHA, and associate with Argonaute proteins, satisfying microRNA attributes. …”
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Genome-wide identification, in silico characterization and expression analysis of the RNA helicase gene family in chickpea (C. arietinum L.) by Sheel Yadav, Yashwant K. Yadava, Deshika Kohli, Shashi Meena, Gopal Kalwan, C. Bharadwaj, Kishor Gaikwad, Ajay Arora, P. K. Jain

“…We hypothesize that this gene could possibly be involved in regulation of DNA methylation levels in chickpea by regulating siRNA production, in conjunction with other proteins like the Argonaute, RNA dependent RNA polymerases and Dicer-like proteins.…”
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Nuclear receptor RXRα binds the precursor of miR-103 to inhibit its maturation by Xiaohong Ye, Yun Yang, Jiayue Yao, Mo Wang, Yixin Liu, Guobin Xie, Zhiping Zeng, Xiao-kun Zhang, Hu Zhou

“…Abstract Background The maturation of microRNAs (miRNAs) successively undergoes Drosha, Dicer, and Argonaute ˗mediated processing, however, the intricate regulations of the individual miRNA maturation are largely unknown. …”
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