Use of CRISPR‐Cas tools to engineer Trichoderma species by Ying Wang, Hongyu Chen, Liang Ma, Ming Gong, Yingying Wu, Dapeng Bao, Gen Zou

Get full text

Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing by Jianli Tao, Daniel E. Bauer, Roberto Chiarle

“…CRISPR-Cas tools have shown exceptional promise in genome engineering over the past decade. …”
Get full text

CRISPR/Cas tools for enhancing the biopreservation ability of lactic acid bacteria in aquatic products by Huina Dong, Huiying Wang, Shaoping Fu, Dawei Zhang, Dawei Zhang

Get full text

Next-Generation Molecular Diagnostics Development by CRISPR/Cas Tool: Rapid Detection and Surveillance of Viral Disease Outbreaks by Sonal Srivastava, Dilip J. Upadhyay, Ashish Srivastava

Get full text

CRISPR-Cas genome editing system: A versatile tool for developing disease resistant crops by Ashwini Talakayala, Srinivas Ankanagari, Mallikarjuna Garladinne

Subjects: “…CRISPR/Cas tool…”
Get full text

Application of different types of CRISPR/Cas-based systems in bacteria by Zhenquan Liu, Huina Dong, Yali Cui, Lina Cong, Dawei Zhang

“…However, the intrinsic toxicity of Cas9 and Cas12a-mediated CRISPR/Cas tools can lead to cell death in some strains, which led to the development of endogenous type I and III CRISPR/Cas systems. …”
Get full text

CRISPR towards a Sustainable Agriculture by Francesco Camerlengo, Arianna Frittelli, Riccardo Pagliarello

“…Genome editing and, in particular, clustered regularly interspaced palindromic repeats/CRISPR-associated protein (CRISPR/Cas) tools will play a major role in plant breeding to address these concerns. …”
Get full text

The Many (Inter)faces of Anti-CRISPRs: Modulation of CRISPR-Cas Structure and Dynamics by Mechanistically Diverse Inhibitors by Helen B. Belato, George P. Lisi

“…The discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPRs (Acrs), has enabled the development of highly controllable and precise CRISPR-Cas tools. Anti-CRISPRs share very little structural or sequential resemblance to each other or to other proteins, which raises intriguing questions regarding their modes of action. …”
Get full text

Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing by Yuyi Tang, Yan Fu

“…The expanding and concerted usage of the CRISPR/Cas tools is making them more powerful in genome editing and other biotechnology applications.…”
Get full text

CRISPR/Cas Technologies and Their Applications in Escherichia coli by Huina Dong, Huina Dong, Yali Cui, Yali Cui, Dawei Zhang, Dawei Zhang, Dawei Zhang

“…Here, the research progress on the most widely used CRISPR/Cas tools and their applications in Escherichia coli is summarized. …”
Get full text

Development and Application of CRISPR/Cas in Microbial Biotechnology by Wentao Ding, Wentao Ding, Yang Zhang, Shuobo Shi

“…Following an overview of various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing, we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening. …”
Get full text

Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives by Wendy Dong, Boris Kantor

“…It is thus reasonable that substantial effort has been allocated towards the development of the improved and optimized LV systems for effective and accurate gene-to-cell transfer of CRISPR/Cas tools. The main effort on that end has been put towards the improvement and optimization of the vector’s expression, development of integrase-deficient lentiviral vector (IDLV), aiming to minimize the risk of oncogenicity, toxicity, and pathogenicity, and enhancing manufacturing protocols for clinical applications required large-scale production. …”
Get full text

CRISPR/Cas-Based Approaches to Study Schizophrenia and Other Neurodevelopmental Disorders by Artemiy O. Kurishev, Dmitry S. Karpov, Nonna I. Nadolinskaia, Anna V. Goncharenko, Vera E. Golimbet

“…CRISPR/Cas systems provide the desired complexity, versatility, and flexibility to create novel genetic tools capable of both altering the DNA sequence and affecting its function at higher levels of genetic information flow. CRISPR/Cas tools make it possible to find and investigate the intricate relationship between the genotype and phenotype of neuronal cells. …”
Get full text

Multiplex genome engineering in Clostridium beijerinckii NCIMB 8052 using CRISPR-Cas12a by Constantinos Patinios, Stijn T. de Vries, Mamou Diallo, Lucrezia Lanza, Pepijn L. J. V. Q. Verbrugge, Ana M. López-Contreras, John van der Oost, Ruud A. Weusthuis, Servé W. M. Kengen

“…Several genome engineering techniques have been developed including the development of numerous CRISPR-Cas tools. Here, we expanded the CRISPR-Cas toolbox and developed a CRISPR-Cas12a genome engineering tool in Clostridium beijerinckii NCIMB 8052. …”
Get full text

Experimental models and tools to tackle glioblastoma by Faye L. Robertson, Maria-Angeles Marqués-Torrejón, Gillian M. Morrison, Steven M. Pollard

“…We also discuss the recent convergence of two key technologies: human stem cell and cancer stem cell culture, as well as CRISPR/Cas tools for precise genome manipulations. New functional genetic approaches in tailored models will likely fuel new discoveries, new target identification and new therapeutic strategies to tackle GBM.…”
Get full text

Multiplex Genome Engineering Methods for Yeast Cell Factory Development by Koray Malcı, Koray Malcı, Laura E. Walls, Laura E. Walls, Leonardo Rios-Solis, Leonardo Rios-Solis

“…This review focuses on recent advancements in such tools, such as delta integration and rDNA cluster integration coupled with CRISPR-Cas tools to greatly enhance multi-integration efficiency. …”
Get full text

A comprehensive appraisal of mechanism of anti-CRISPR proteins: an advanced genome editor to amend the CRISPR gene editing by Nisha Choudhary, Dipty Tandi, Rakesh Kumar Verma, Virendra Kumar Yadav, Naveen Dhingra, Tathagata Ghosh, Mahima Choudhary, Rajarshi K. Gaur, Magda H. Abdellatif, Amel Gacem, Lienda Bashier Eltayeb, Mohammed S. Alqahtani, Mohammed S. Alqahtani, Krishna Kumar Yadav, Krishna Kumar Yadav, Byong-Hun Jeon

“…The development of precise and controlled CRISPR-Cas tools has been made possible by the discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPRs (Acrs). …”
Get full text

Effective gene silencing using type I–E CRISPR system in the multiploid, radiation-resistant bacterium Deinococcus radiodurans by Chitra S. Misra, Neha Pandey, Deepti Appukuttan, Devashish Rath

“…In view of this, the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas tools provide a large repertoire of applications for gene manipulation. …”
Get full text

Bioinformatics Identification of Anti-CRISPR Loci by Using Homology, Guilt-by-Association, and CRISPR Self-Targeting Spacer Approaches by Yanbin Yin, Bowen Yang, Sarah Entwistle

“…As the natural off-switch of CRISPR-Cas systems, anti-CRISPRs have a great potential to serve as regulators of CRISPR-Cas tools and enable safer and more controllable genome editing. …”
Get full text

Development of a CRISPR-Cas12a system for efficient genome engineering in clostridia by Yanchao Zhang, Aleksandra M. Kubiak, Tom S. Bailey, Luuk Claessen, Philip Hittmeyer, Ludwig Dubois, Jan Theys, Philippe Lambin

“…In this study, we expanded the CRISPR-Cas tools in clostridia by establishing a CRISPR-Cas12a system with two different cas12a genes (Ascas12a from Acidaminococcus and Fncas12a from Francisella novicida). …”
Get full text