Thyme and Oregano Oil Potential Therapeutics against Malathion Toxicity through Biochemical, Histological, and Cytochrome P450 1A2 Activities in Male Wistar Rats by Fatimah A. Al-Saeed, Montaser Elsayed Ali

“…However, the results indicated that TEO was more effective than OEO in increasing CYP1A2 expression and alleviating MOP-induced toxicity. …”
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In vitro evaluation of fenfluramine and norfenfluramine as victims of drug interactions by Parthena Martin, Maciej Czerwiński, Pallavi B. Limaye, Seema Muranjan, Brian W. Ogilvie, Steven Smith, Brooks Boyd

“…Incubation of nFFA with rCYP1A2, rCYP2B6, rCYP2C19, and rCYP2D6 resulted in 10%−20% metabolism and no clear inhibition of nFFA metabolism by any CYP‐selective inhibitor. …”
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Bioactivities of berberine metabolites after transformation through CYP450 isoenzymes by Li Zhuo-Rong, Yi Hong, Li Ying-Hong, Wang Yue-Ming, Yang Peng, Kong Wei-Jia, Wang Yan-Xiang, Ren Gang, Li Yi, Song Dan-Qing, Jiang Jian-Dong

“…In the cell-free transformation reactions, M2 and M3 were detectable after incubating BBR with rCYP450s or human liver microsomes; however, M1 and M4 were below detective level. CYP2D6 and CYP1A2 played a major role in transforming BBR into M2; CYP2D6, CYP1A2 and CYP3A4 were for M3 production. …”
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Bioactivation, Mutagenicity, DNA Damage, and Oxidative Stress Induced by 3,4-Dimethylaniline by Mariam R. Habil, Raúl A. Salazar-González, Mark A. Doll, David W. Hein

“…In CHO cells expressing CYP1A2 and NAT1, 3,4-DMA caused concentration-dependent increases in reactive oxygen species (ROS), double-stranded DNA damage, and HPRT mutations. …”
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IRE1α activation protects mice against acetaminophen-induced hepatotoxicity by Hur, Kyu Yeon, So, Jae-Seon, Frank-Kamenetsky, Maria, Fitzgerald, Kevin, Kotelianski, Victor E., Iwawaki, Takao, Lee, Ann-Hwee, Ruda, Vera, Glimcher, Laurie H

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Induction of cytochrome P450 via upregulation of CAR and PXR: a potential mechanism for altered florfenicol metabolism by macranthoidin B in vivo by Si-cong Li, Si-cong Li, Bin Wang, Bin Wang, Min Zhang, Min Zhang, Qin Yin, Zi-yi Yang, Zi-yi Yang, Xu-ting Li, Xu-ting Li, Ge Liang, Ge Liang

“…Hepatic CYP1A2 and CYP2C11 activities were measured using a cocktail method. …”
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Evaluation of the inhibition performance of pyrrole derivatives against CYP450 isoforms by Magdalena Kondeva-Burdina, Emilio Mateev, Ali Irfan, Hristina Kuteva, Maya Georgieva

“…The aim of this study was to conduct in silico and in vitro activity assessments of recently synthesized pyrrole-based compounds against three major CYP450 isoforms – CYP1A2, CYP2D6 and CYP3A4. The in vitro study contained specific cytochrome P450 isoform inhibitors and substrates (for CYP1A2, CYP2D6, and CYP3A4) to determine the inhibition performance of the tile compounds at 1 µM concentration. …”
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Safety issues regarding melatonin use in child and adolescent patients with sleep problems by Eunsoo Moon, Jung Hyun Lee

“…It is necessary to monitor potential drug interactions with medications such as inhibitors and enhancers of cytochrome P450 1A2 (CYP1A2). Furthermore, low CYP1A2 expression in young children requires proper dose adjustment. …”
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代谢活化系统在人细胞转化模型中的应用

“…【目的】 探讨不同的代谢活化系统在人细胞转化模型中潜在的应用价值65377; 【方法】 在永生化人肝细胞L02中导入第12密码子突变的H-RAS癌基因(L02-R细胞),使其处于恶性转化前的易感状态65377;选择具有代表性的间接致癌物黄曲霉毒素B1 (aflatoxin B1, AFB1) 作用于永生化和转化前期细胞株,采用三种代谢系统:高表达代谢酶P450 CYP1A2,低剂量底物诱导和加入经典的大鼠S9代谢辅助系统,通过软琼脂克隆形成试验和裸鼠皮下成瘤试验来比较在不同的代谢活化系统下,间接致癌物诱导细胞转化的效能65377; 【结果】 蛋白印迹验证H-Ras稳定高表达的转基因细胞株构建成功65377;通过Ames试验,蛋白印记和CYP1A2酶活性等方法比较三种代谢系统酶活性65377;在未加代谢系统的条件下,经AFB1染毒后第17周L02-R细胞发生转化,而对照组细胞L02-V在染毒20周未能发生转化65377;而加入S9代谢系统时,AFB1诱导L02-R细胞在第8周发生转化,比未加代谢系统缩短了9周;经低剂量0.1 μmol/L AFB1诱导和高表达CYP1A2均使L02-R的转化间期缩短6周,于第11周发生转化65377; 【结论】 三种代谢系统都能够促进间接致癌物AFB1的代谢活化,缩短细胞转化的时间,提高细胞转化效率65377;与传统的S9代谢活化系统相比,低剂量诱导作为新的代谢活化的方法在细胞转化试验中有着良好的应用前景65377;…”
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The Reduction of the Combined Effects of Aflatoxin and Ochratoxin A in Piglet Livers and Kidneys by Dietary Antioxidants by Roua Gabriela Popescu, Sorin Avramescu, Daniela Eliza Marin, Ionelia Țăranu, Sergiu Emil Georgescu, Anca Dinischiotu

“…The purpose of this study was to investigate the combined effects of aflatoxin B1 and ochratoxin A on protein expression and catalytic activities of CYP1A2, CYP2E1, CYP3A29 and GSTA1 and the preventive effect of dietary byproduct antioxidants administration against these mycotoxin damage. …”
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Effects of Chronic Alcohol Intake on the Composition of the Ensemble of Drug-Metabolizing Enzymes and Transporters in the Human Liver by Kari A. Gaither, Guihua Yue, Dilip Kumar Singh, Julia Trudeau, Kannapiran Ponraj, Nadezhda Y. Davydova, Philip Lazarus, Dmitri R. Davydov, Bhagwat Prasad

“…Tobacco smokers showed elevated CYP1A2, UGT1A6, and UGT2B4 and reduced FMO3, FMO4, and FMO5 levels, while in females, CYP1A2, UGT2B17, and UGT2B15 levels were lower, and UGT2A3 and STS were higher compared to males. …”
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Dietary Resveratrol Alleviates AFB1-Induced Ileum Damage in Ducks via the Nrf2 and NF-κB/NLRP3 Signaling Pathways and CYP1A1/2 Expressions by Hao Yang, Yingjie Wang, Chunting Yu, Yihan Jiao, Ruoshi Zhang, Sanjun Jin, Xingjun Feng

“…The expression of genes, including CYP1A2, CYP2A6, and CYP3A4, at the mRNA level was significantly upregulated (<i>p</i> < 0.05) by AFB₁. …”
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Inhibitory effects of herbal medicines with claimed anticancer indications on cytochrome P450—An evaluation of drug-herb interactions risk by Misheck Mudyiwa, Manju Sharma, Samarendra Kumar Ray, Collen Masimirembwa, Roslyn Stella Thelingwani

“…Drugs that are substrates of CYP1A2 should be administered with caution for patients who are taking the herbs which have been studied in this research as risk for drug toxicities may be high. …”
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Mertansine Inhibits mRNA Expression and Enzyme Activities of Cytochrome P450s and Uridine 5′-Diphospho-Glucuronosyltransferases in Human Hepatocytes and Liver Microsomes by Won-Gu Choi, Ria Park, Dong Kyun Kim, Yongho Shin, Yong-Yeon Cho, Hye Suk Lee

“…A 48 h treatment of mertansine (1.25&#8722;2500 nM) in human hepatocytes resulted in the dose-dependent suppression of mRNA levels of CYP1A2, CYP2B6, CYP3A4, CYP2C8, CYP2C9, CYP2C19, UGT1A1, and UGT1A9, with IC₅₀ values of 93.7 109.1, 36.8 18.3, 160.6 167.4, 32.1 14.9, 578.4 452.0, 539.5 233.4, 856.7 781.9, and 54.1 29.1 nM, respectively, and decreased the activities of CYP1A2-mediated phenacetin <i>O</i>-deethylase, CYP2B6-mediated bupropion hydroxylase, and CYP3A4-mediated midazolam 1-hydroxylase. …”
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In Vitro Inhibitory Effects of Scutellarin on Six Human/Rat Cytochrome P450 Enzymes and P-glycoprotein by Yong-Long Han, Dan Li, Quan-Jun Yang, Zhi-Yong Zhou, Li-Ya Liu, Bin Li, Jin Lu, Cheng Guo

“…In this study, the in vitro inhibitory effects of scutellarin on six major human CYPs (CYP1A2, CYP2C8, CYP2C9, CYP2C19, CYP2D6, and CYP3A4) and six rat CYPs (CYP1A2, CYP2C7, CYP2C11, CYP2C79, CYP2D4, and CYP3A2) activities were examined by using liquid chromatography-tandem mass spectrometry. …”
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Impact of Pharmacogenetic Testing on Clozapine Treatment Efficacy in Patients with Treatment-Resistant Schizophrenia by Estela Sangüesa, Emilio Fernández-Egea, Julia Concha, Cristina B. García, María Pilar Ribate

“…Genetic associations were explored, highlighting <i>SLC6A4, HTR2A</i>, and the *1F/*1F polymorphism for the <i>CYP1A2</i> gene.…”
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Effect of Honokiol on Cytochrome P450 and UDP-Glucuronosyltransferase Enzyme Activities in Human Liver Microsomes by Yong Yeon Cho, Jae Young Lee, Jun-Ho Choi, Sung Hum Yeon, Sung-Woon Hong, Soon Sang Kwon, Tae Yeon Kong, Hyeon-Uk Jeong, Hye Suk Lee

“…These in vitro results indicate that honokiol has the potential to cause pharmacokinetic drug interactions with other co-administered drugs metabolized by CYP1A2, CYP2C8, CYP2C9, CYP2C19, and UGT1A9.…”
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Gene Silencing and Haploinsufficiency of Csk Increase Blood Pressure. by Hyeon-Ju Lee, Ji-One Kang, Sung-Moon Kim, Su-Min Ji, So-Yon Park, Marina E Kim, Baigalmaa Jigden, Ji Eun Lim, Sue-Yun Hwang, Young-Ho Lee, Bermseok Oh

“…Injection of siRNAs for mouse homologs Csk, Ulk3, and Cyp1a2 (negative control) showed reduced target gene mRNA levels in vivo. …”
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Sex differences in CYP450-based sodium dehydroacetate metabolism and its metabolites in rats by Meng Zhang, Pengfei Du, Yirong Xiao, Hao Liu, Meixue Wang, Yumei Zhang, Xin Chen

“…Here, we identified several Cytochrome P450 (CYP450) sub-enzymes involved in sex differences related to DHA-Na metabolism, along with two related DHA-Na metabolites. CYP1A2, CYP3A2, and CYP2D1 were primarily responsible for DHA-Na metabolism, which was stronger in male rats than in female rats. …”
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استخلاص الكافئين ومستقلبه الباراكزانيتن من البول ومقايستهما بواسطة جهاز الكروموتوغرافيا السائلة عالية الأداء... by د. سمر الزير

“…يستقلب الكافئين بواسطة أنزيم السيتوكروم CYP1A2 إلى مستقلبه الأساسي الباراكزانيتن. هدف هذا البحث إلى دراسة مصدوقية طريقة استخلاص وتحليل الكافئين والباراكزانتين معاً في البول بواسطة جهاز الكروموتوغرافيا السائلة عالية الأداء HPLC كونه جهاز متوافر. …”
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