Investigation of solute concentrations in a 3D model of intervertebral disc. by Mokhbi Soukane, D, Shirazi-Adl, A, Urban, J

“…Effects of perturbations (calcification, sclerosis) in endplates, increases in cell metabolic rates following growth factor injection and changes in lumbar posture (kyphotic or lordotic) on extreme values of nutrient and metabolite concentrations and their spatial locations are investigated. …”

ATP Consumption Promotes Cancer Metabolism by Israelsen, William James, Vander Heiden, Matthew G.

“…Cancer cells metabolize glucose by aerobic glycolysis, a phenomenon known as the Warburg effect. …”
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The effect of electrical stimulation on the osteogenesis of MC3T3-E1 cells by Tan, Lynette Sze Luan

“…The enhancement on cell metabolic activity and cell proliferation indicated biocompatibility of PCLTCP+PEDOT-GOPS film with or without PEMF exposure. …”
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Amino acid sensing by mTORC1: Intracellular transporters mark the spot. by Goberdhan, D, Wilson, C, Harris, A

“…Cell metabolism and growth are matched to nutrient availability via the amino acid-regulated mechanistic Target of Rapamycin Complex 1 (mTORC1). …”

Amino acid sensing by mTORC1: intracellular transporters mark the spot by Goberdhan, D, Wilson, C, Harris, A

“…Cell metabolism and growth are matched to nutrient availability via the amino-acid-regulated mechanistic target of rapamycin complex 1 (mTORC1). …”

Three-dimensional fabrication of engineered bone in rotating wall vessel bioreactor by Song, K, Liu, T, Li, X, Cui, Z, Ge, D, Sun, X, Ma, X

“…The biological properties of the fabricated bone tissue were detected by inverted microscope, scanning electron microscope (SEM), alkaline phosphatase (ALP), von-Kossa staining on mineralized nodules and AO/EB fluorescence staining. Meanwhile the cells' metabolism of nutrients was monitored and analyzed during the whole culture process. …”

An in line non-invasive optical system to monitor pH in cell and tissue culture. by Xu, X, Smith, S, Urban, J, Cui, Z

“…pH is an important control parameter for maintenance of cell viability and for improving tissue functions during cell and tissue culture. pH monitoring during cell/tissue culture also provides valuable information on cell metabolic processes and living environment. In this study, an on-line, non-invasive pH monitoring system was developed for use during tissue/cell culture in a perfusion system, using an optical method. …”

α-cell glucokinase suppresses glucose-regulated glucagon secretion by Basco, D, Zhang, Q, Salehi, A, Tarasov, A, Dolci, W, Herrera, P, Spiliotis, I, Berney, X, Tarussio, D, Rorsman, P, Thorens, B

“…Thus, glucokinase governs an α-cell metabolic pathway that suppresses secretion at or above normoglycemic levels; abnormal suppression of glucagon secretion deregulates hepatic glucose metabolism and, over time, induces a pre-diabetic phenotype.…”

Akt regulation of glycolysis mediates bioenergetic stability in epithelial cells by Hung, Yin P, Teragawa, Carolyn, Kosaisawe, Nont, Gillies, Taryn E, Pargett, Michael, Minguet, Marta, Distor, Kevin, Rocha-Gregg, Briana L, Coloff, Jonathan L, Yellen, Gary, Brugge, Joan S, Albeck, John G, Keibler, Mark Andrew, Stephanopoulos, Gregory

“…By monitoring single-cell dynamics in each of these contexts, we identified PI3K/Akt regulation of glycolysis as a multifaceted modulator of single-cell metabolic dynamics that is required to maintain metabolic stability in proliferating cells.…”
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Evaluation of biological response on microparticle-coated surfaces by Ong, Cheng Ji

“…PLGA microparticles carrying different small molecules (Stattic, Fludarabine) were coated on breast implant membranes, and placed in direct contact with L929 fibroblast cells, and tested for cell metabolic activity as well as cell viability at different time points, D1, D3, and D5. …”
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Dynamic IL-6R/STAT3 signaling leads to heterogeneity of metabolic phenotype in pancreatic ductal adenocarcinoma cells by Blaszczak, W, White, B, Monterisi, S, Swietach, P

“…Cancer cells could avoid this by rationing resources, e.g., dynamically switching between “baseline” and “activated” metabolic states. Using single-cell metabolic phenotyping of pancreatic ductal adenocarcinoma cells, we identify MIA-PaCa-2 as having broad heterogeneity of fermentative metabolism. …”

Bridging substrate intake kinetics and bacterial growth phenotypes with flux balance analysis incorporating proteome allocation by Zeng, H, Yang, A

“…In this work, we integrated proteome allocation principles into a Flux Balance Analysis (FBA) model of Escherichia coli, which quantitatively revealed potential mechanisms that underpin the phenomenological Monod parameters: the maximum specific growth rate could be dictated by the abundance of growth-controlling proteome and growth-pertinent proteome cost; more importantly, the Monod constant (Ks) was shown to relate to the Michaelis constant for substrate transport (Km,g), with the link being dependent on the cell's metabolic strategy. Besides, the proposed model was able to predict glucose uptake rate at given external glucose concentration through the size of available proteome resource for substrate transport and its enzymatic cost, while growth rate and acetate overflow were accurately simulated for two E. coli strains. …”

Phosphatidylinositol 4-kinase serves as a metabolic sensor and regulates priming of secretory granules in pancreatic beta cells. by Olsen, H, Hoy, M, Zhang, W, Bertorello, A, Bokvist, K, Capito, K, Efanov, A, Meister, B, Thams, P, Yang, SN, Rorsman, P, Berggren, P, Gromada, J

“…Insulin secretion is controlled by the beta cell's metabolic state, and the ability of the secretory granules to undergo exocytosis increases during glucose stimulation in a membrane potential-independent fashion. …”

Targeting metabolic flexibility via angiopoietin-like 4 protein sensitizes metastatic cancer cells to chemotherapy drugs by Lim, Maegan Miang Kee, Wee, Jonathan Wei Kiat, Soong, Jen Chi, Chua, Damien, Tan, Wei Ren, Lizwan, Marco, Li, Yinliang, Teo, Ziqiang, Goh, Wilson Wen Bin, Zhu, Pengcheng, Tan, Nguan Soon

“…ANGPTL4 empowered cancer cells metabolic flexibility during EMT, securing ample cellular energy that fuels multiple ABC transporters to confer EMT-mediated chemoresistance. …”
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Effect of laser irradiation on cell function and its implications in Raman spectroscopy by Yuan, X, Song, Y, Song, Y, Xu, J, Wu, Y, Glidle, A, Cusack, M, Ijaz, U, Cooper, J, Huang, W, Yin, H

“…Laser irradiation could compromise the physiological function of cells, and the degree of destruction is both dose and strain dependent, ranging from reduced cell growth to a complete loss of cell metabolic activity and finally to physical disintegration. …”

3D bone tissue growth in hollow fibre membrane bioreactor: implications of various process parameters on tissue nutrition. by Abdullah, N, Das, D, Ye, H, Cui, Z

“…The model parameters (e.g., cell metabolic rates) and operating conditions for the mathematical model have been obtained from, or correspond to, in-house experiments with the exception of a few variables which have been taken from the literature. …”

Cryopreservation of human bone marrow-derived mesenchymal stem cells with reduced dimethylsulfoxide and well-defined freezing solutions. by Liu, Y, Xu, X, Ma, X, Martin-Rendon, E, Watt, S, Cui, Z

“…We measured the post-thawing cell viability and early apoptotic behaviors, cell metabolic activities, and growth dynamics. Cell morphology and osteogenic, adipogenic and chondrogenic differentiation capability were also tested after cryopreservation. …”

The effects of 1-131 on different types of cancer Cell lines by Yusoff, Nik Nur Nadiah Nik

“…The findings of this study suggest that the cellular uptake of 1-131, cells viability and proliferation were highly dependent on cells metabolism, mitotic rate, occurrence of apoptosis, doubling time, cells radiosensitivity, and so on with consideration of some limitations.…”
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Perfusion culture enhanced human endometrial stromal cell growth in alginate-multivalent integrin α5β1 ligand scaffolds. by Li, Z, Kreiner, M, Edrada-Ebel, R, Cui, Z, van der Walle, C, Mardon, H

“…Conjugation of alginate to FN was an absolute requirement to attenuate the loss of cell metabolic activity over 7 days culture. ECM deposition for blank alginate and alginate-monomeric FN was similar, but increased around 2-fold and 3-fold for alginate-tetrameric FN under static and perfusion culture, respectively. …”

Installation of C4 photosynthetic pathway enzymes in rice using a single construct by Ermakova, M, Arrivault, S, Giuliani, R, Danila, F, Alonso-Cantabrana, H, Vlad, D, Ishihara, H, Feil, R, Guenther, M, Borghi, GL, Covshoff, S, Ludwig, M, Cousins, AB, Langdale, JA, Kelly, S, Lunn, JE, Stitt, M, von Caemmerer, S, Furbank, RT

“…Introduction of a C4 photosynthetic mechanism into C3 crops offers an opportunity to improve photosynthetic efficiency, biomass and yield in addition to potentially improving nitrogen and water use efficiency. To create a two-cell metabolic prototype for an NADP-malic enzyme type C4 rice, we transformed Oryza sativa spp. japonica cultivar Kitaake with a single construct containing the coding regions of carbonic anhydrase, phosphoenolpyruvate (PEP) carboxylase, NADP-malate dehydrogenase, pyruvate orthophosphate dikinase and NADP-malic enzyme from Zea mays, driven by cell-preferential promoters. …”