Installation of C4 photosynthetic pathway enzymes in rice using a single construct by Ermakova, M, Arrivault, S, Giuliani, R, Danila, F, Alonso-Cantabrana, H, Vlad, D, Ishihara, H, Feil, R, Guenther, M, Borghi, GL, Covshoff, S, Ludwig, M, Cousins, AB, Langdale, JA, Kelly, S, Lunn, JE, Stitt, M, von Caemmerer, S, Furbank, RT

“…Introduction of a C4 photosynthetic mechanism into C3 crops offers an opportunity to improve photosynthetic efficiency, biomass and yield in addition to potentially improving nitrogen and water use efficiency. To create a two-cell metabolic prototype for an NADP-malic enzyme type C4 rice, we transformed Oryza sativa spp. japonica cultivar Kitaake with a single construct containing the coding regions of carbonic anhydrase, phosphoenolpyruvate (PEP) carboxylase, NADP-malate dehydrogenase, pyruvate orthophosphate dikinase and NADP-malic enzyme from Zea mays, driven by cell-preferential promoters. …”

Effect of electrical stimulation and scaffold architecture on osteogenesis of osteogenic cells by Leong, Joshua Wei Ren

“…In this paper, the effects of PVDF coating under PEMF treatment of 0.6 mT, at 50 Hz frequency in 3D-printed PCL-TCP scaffolds, on the proliferation and mineralization of osteoblast precursor cells MC3T3-E1 were investiaged. Cell metabolic activity was assessed by alamarBlue time-course measurements and results indicated an increased in metabolic activity with electroactive material under PEMF exposure. …”
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In vitro investigations on the effects of graphene and graphene oxide on polycaprolactone bone tissue engineering scaffolds by Hou, Yanhao, Wang, Weiguang, Bartolo, Paulo

“…However, the variations in compressive modulus, strength, surface hardness, and cell metabolic activity strongly depended on the type of reinforcement. …”
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Exploiting stable isotope imaging with high resolution secondary ion mass spectrometry for applications in biology by Jiang, H

“…Working with collaborators in the UK and USA, I have applied NanoSIMS analysis to study 3 research areas, including molecule interactions, single cell metabolisms and lipid imaging in tissues. Antimicrobial peptides (AMPs) play important role in the immune system, and understanding how AMPs interact with cell membranes can provide useful information to design new therapies to control infection. …”

Iron controls the adaptive immune response by Frost, J

“…Here we demonstrate that genetic defects in iron homeostasis, such as the TfrcY20H/Y20H mutation and IRP1/2 deficiency, cell-intrinsically disrupt adaptive immunity. We find that T-cell metabolic activity including mitochondrial ATP generation is impaired by limiting iron acquisition in vitro, concomitant with reduced proliferation, suggesting that iron is a key ‘immunometabolite’. …”

Three-dimensional fabrication of engineered bone with human bio-derived bone scaffolds in a rotating wall vessel bioreactor. by Song, K, Liu, T, Cui, Z, Li, X, Ma, X

“…After the assessment of the biological functions of the expanded cells by histomorphometry, the cells were seeded at 2 x 10(6) and 1 x 10(6) cells/mL, respectively, onto the 3D human BDBS and cultured for 3 weeks in the RWVB. The cells metabolism and nutrient concentration were monitored in the whole culture processes. …”

β-Phase poly(vinylidene fluoride) films encouraged more homogeneous cell distribution and more significant deposition of fibronectin towards the cell–material interface compared to... by Low, Yuen Kei Adarina, Zou, Xi, Fang, Yuming, Wang, Junling, Lin, Weisi, Ng, Kee Woei, Boey, Freddy Yin Chiang

“…Our results showed that β-phase PVDF films with the largest area expressing piezoelectric effect elicited highest cell metabolic activity at day 3 of culture. Increased fibronectin adsorption towards the cell-material interface was shown on β-phase PVDF films. …”
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Single cell metabolite detection using inertial microfluidics-assisted ion mobility mass spectrometry by Zhang, Leicheng, Xu, Tengfei, Zhang, Jingtao, Wong, Stephen Choong Chee, Ritchie, Mark, Hou, Han Wei, Wang, Yulan

“…This analytical system enables six to eight single-cell metabolic fingerprints to be collected per minute, including gas-phase collisional cross section (CCS) measurements as an additional molecular descriptor, giving increased confidence in metabolite identification. …”
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Microscale controlled continuous cell culture by Lee, Kevin Shao-Kwan

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Optimization of [superscript 13]C isotopic tracers for metabolic flux analysis in mammalian cells by Walther, Jason L., Metallo, Christian M., Zhang, Jie, Stephanopoulos, Gregory

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A bioactive injectable bulking material; a potential therapeutic approach for stress urinary incontinence by Vardar, E., Vythilingam, Ganesh, Pinnagoda, K., Engelhardt, E.M., Zambelli, P.Y., Hubbell, J.A., Lutolf, M.P., Frey, P., Larsson, H.M.

“…The fib_rIGF-1 showed no adverse effect on human smooth muscle cell metabolic activity and viability in vitro based on AlamarBlue assays and Live/Dead staining. …”

The development and optimization of processes for the expression of sialylated recombinant human therapeutic glycoprotein in insect cell-baculovirus system. by Mohd. Hani, Mohamed Khalil

“…Unfortunately the Nglycan structure of the recombinant glycoprotein produced in insect cell culture is slightly different from the ones naturally produced in human, usually unsialylated and sometimes nongalactosylated, suggesting the nonexistence of several genes in insect cells. Metabolic engineering is one of the approaches to mimic the biosynthesis pathway that is naturally occurring in human. …”
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Preliminary characterization of a polycaprolactone-surgihoneyRO electrospun mesh for skin tissue engineering by Aslan, Enes, Vyas, Cian, Mieles, Joel Yupanqui, Humphreys, Gavin, Diver, Carl, Bartolo, Paulo

“…The biocompatibility of the meshes was assessed using human dermal fibroblasts and adipose-derived stem cells, which showed comparable or higher cell metabolic activity and viability for SurgihoneyRO-containing meshes compared to polycaprolactone only meshes. …”
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Effects of sialic acid from edible bird nest on cell viability associated with brain cognitive performance in mice by Abdul Khalid, Siti Khadijah, Abd Rashed, Aswir, Abdul Aziz, Saleha, Ahmad, Hafandi

“…The EBN is widely consumed due to its higher nutritional contents and antioxidant status; however, an interaction of EBN on brain cell metabolic activity and viability are still unclear. …”
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Effect of elevated glucose on primary human tenocytes and implications for diabetic tendinopathy by Kalivodova, Z

“…Extracellular glucose affected cellular response to H₂O₂: 1 mM H₂O₂-treated tenocytes in high glucose media had cell metabolic activity significantly decreased compared to iden- tical H₂O₂ treatment in low glucose media. …”

High-throughput metabolomics reveals dysregulation of hydrophobic metabolomes in cancer cell lines by Eleusine indica by Perng Yang Puah, Jiunn, Dexter Herng Lee, Soo, Huan Puah, Nik Amin Sahid Nik Lah, Yee, Soon Ling, Siat, Yee Fong

“…The present study investigated the anticancer effects of a root fraction of E. indica (R-S5-C1-H1) on H1299, MCF-7, and SK-HEP-1 cell lines and analyzed metabolic changes in the treated cancer cells using ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS). Cell metabolic activity assays demonstrated that the cell viability of the three cancer cell lines was significantly reduced following treatment with R-S5-C1-H1, with half-maximal inhibitory concentrations values of 12.95 µg/mL, 15.99 µg/mL, and 13.69 µg/mL at 72 h, respectively. …”
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ERO1α promotes hypoxic tumor progression and is associated with poor prognosis in pancreatic cancer by Gupta, Nikhil, Park, Jung Eun, Tse, Wilford, Low, Jee Keem, Kon, Oi Lian, McCarthy, Neil, Sze, Siu Kwan

“…Using this method, we identified that hypoxia stress stimulates pancreatic cancer cells to rapidly translate proteins that enhance metastasis (NOTCH2, NCS1, CD151, NUSAP1), treatment resistance (ABCB6), immune suppression (NFIL3, WDR4), angiogenesis (ANGPT4, ERO1α, FOS), alter cell metabolic activity (HK2, ENO2), and mediate growth-promoting cytokine responses (CLK3, ANGPTL4). …”
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High‐throughput metabolomics reveals dysregulation of hydrophobic metabolomes in cancer cell lines by Eleusine indica by Perng Yang Puah, Jiunn, Dexter Herng Lee, Soo, Huan Puah, Nik Amin Sahid Nik Lah, Yee, Soon Ling, Fong, Alison Siat Yee

“…The present study investigated the anticancer effects of a root fraction of E. indica (R-S5-C1-H1) on H1299, MCF-7, and SK-HEP-1 cell lines and analyzed metabolic changes in the treated cancer cells using ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS). Cell metabolic activity assays demonstrated that the cell viability of the three cancer cell lines was significantly reduced following treatment with R-S5-C1-H1, with half-maximal inhibitory concentrations values of 12.95 µg/mL, 15.99 µg/mL, and 13.69 µg/mL at 72 h, respectively. …”
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High-throughput metabolomics reveals dysregulation of hydrophobic metabolomes in cancer cell lines by Eleusine indica by Puah, Perng Yang, Lee, Dexter Jiunn Herng, Puah, Soo Huan, Nik Amin Sahid Nik Lah, Ling, Yee Soon, Fong, Siat Yee

“…The present study investigated the anticancer effects of a root fraction of E. indica (R-S5-C1-H1) on H1299, MCF-7, and SK-HEP-1 cell lines and analyzed metabolic changes in the treated cancer cells using ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS). Cell metabolic activity assays demonstrated that the cell viability of the three cancer cell lines was significantly reduced following treatment with R-S5-C1-H1, with half-maximal inhibitory concentrations values of 12.95 µg/mL, 15.99 µg/mL, and 13.69 µg/mL at 72 h, respectively. …”
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Cell State Identication by Mass, Density, and volume by Bryan, Andrea K. (Andrea Kristine)

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