Efficient Secretory Production of Lytic Polysaccharide Monooxygenase <i>Ba</i>LPMO10 and Its Application in Plant Biomass Conversion by Xiao Guo, Yajing An, Fuping Lu, Fufeng Liu, Bo Wang

“…Lytic polysaccharide monooxygenases (LPMOs) can oxidatively break the glycosidic bonds of crystalline cellulose, providing more actionable sites for cellulase to facilitate the conversion of cellulose to cello-oligosaccharides, cellobiose and glucose. In this work, a bioinformatics analysis of <i>Ba</i>LPMO10 revealed that it is a hydrophobic, stable and secreted protein. …”
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Comparative analysis of the collection winter barley samples in the Rostov region by E. S. Doroshenko, E. G. Filippov

“…Based on the study results of this material, there were identified new sources for use in breeding programs to increase the values of individual traits:– low stem: ‘Perkins’ (USA), ‘Novosadski 331’ (Yugoslavia);– resistance to lodging: ‘Hobbit’ (Switzerland), ‘Merchant’ (Bulgaria), ‘Akademichesky’ (Ukraine), ‘Gust’ (Belarus), ‘18513 EN11’ (France), ‘Explorer 2’, ‘Explorer 3’, ‘Explorer 4’, ‘Explorer 6’, ‘Explorer 8’, ‘Wintwalt’, ‘Tiffani’ (Germany), ‘Perkins’ (USA), ‘Novosadski 331’ (Yugoslavia), ‘Posaune’, ‘Sombrero’, ‘Karisma’ (England), ‘Duet’ (Germany);– number of productive stems per area unit: ‘Explorer 8’, ‘Explorer 4/2’, ‘Wintwalt’ (Germany), ‘Baraka’ (France), ‘Posaune’ (England);– grain content per head: ‘Artel’ (Russia), ‘Caprice’, ‘Azurel’, ‘Rurdue’ (France), ‘HWV1427’ (Germany);– 1000-grain weight: ‘Randevu’, ‘Premier’ (Russia), ‘Nectaria’, ‘Vanessa’ (France), ‘Explorer 3’ (Germany);– grain weight per head: ‘Randevu’, ‘Pallidum 1916’ (Russia), ‘Oribi’, ‘Bezosty’ (France), ‘Explorer 2’, ‘KWS-Scala’ (Germany), ‘Hobbit’ (Switzerland);– fast-maturity: ‘Parallelum 1963’, ‘Sekret,’ ‘Bezosty 1953’, ‘Bezosty 1954’ (Russia), ‘Fermer’ (Ukraine);– high productivity: ‘Rostovsky 55’, ‘Grand’, ‘Artel’ (Russia), ‘KWS-Hiskory’, ‘Explorer 8’ (Germany), ‘Esterel’ (France).According to the complex of economically valuable traits (4 or more traits), there were identified 15 varieties: ‘Randevu’, ‘Parallelum 1960’ (Russia), ‘KWS-Scala’, ‘KWS-2-117’, ‘KWS-2-234’, ‘Explorer 4’, ‘Explorer 3/2’ (Germany), ‘Baraka’, ‘Rebelle’, ‘6577 CH’, ‘18513 EH11’ (France), ‘Cello’, ‘Fenesse’ (USA), ‘Novosadski 321’ (Yugoslavia), ‘Posaune’ (England).…”
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Modified dietary fiber from cassava pulp affects the cecal microbial population, short-chain fatty acid, and ammonia production in broiler chickens by S. Okrathok, M. Sirisopapong, P. Mermillod, S. Khempaka

“…The M-DFCP contained total dietary fiber (TDF), soluble dietary fiber (SDF), insoluble dietary fiber (IDF), cello-oligosaccharides (COS), and xylo-oligosaccharides (XOS) of approximately 280.70, 22.20, 258.50, 23.93, and 157.55 g/kg, respectively. …”
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Summary a case of endovascular treatment of a patient with tandem occlusions of cerebral arteries caused by a mine-explosive injury by Yu.V. Cherednychenko

“…After double antiplatelet preparation, endovascular recanalization and stenting of the occluded right internal carotid artery was performed via transfemoral access using combined proximal and distal antiembolic protection (Cello 9F balloon guide catheter and SpiderFX distal antiembolic protection device) and with implantation of a carotid stent Protégé. …”
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Characterization of a glucose-tolerant β-glucosidase from Anoxybacillus sp. DT3-1 by Chan, C. S., Sin, L. L., Chan, K. G., Shamsir, M. S., Manan, F. A., Sani, R. K., Goh, K. M.

“…The recombinant DT-Bgl was highly active on cello-oligosaccharides and p-nitrophenyl-β-d-glucopyranoside (pNPG). …”
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Impact of Prophylactic Hydroxychloroquine on People at High Risk of COVID-19: A Systematic Review and Meta-Analysis by Adrian V. Hernandez, John Ingemi, Michael Sherman, Vinay Pasupuleti, Joshuan J. Barboza, Alejandro Piscoya, Yuani M. Roman, Charles M. White

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Identification of New Drug Target in <i>Staphylococcus lugdunensis</i> by Subtractive Genomics Analysis and Their Inhibitors through Molecular Docking and Molecular Dynamic Simulat... by Yahya Alhamhoom, Umme Hani, Fatima Ezzahra Bennani, Noor Rahman, Md Abdur Rashid, Muhammad Naseer Abbas, Luca Rastrelli

“…Various tools, including the DEG (database of essential genes) for the essentiality of proteins, the KEGG for pathways analysis, CELLO V.2.5 for cellular localization prediction, and the drug bank database for predicting the druggability potential of proteins, were used. …”
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Functional characterization of a lytic polysaccharide monooxygenase from Schizophyllum commune that degrades non-crystalline substrates by Heidi Østby, Idd A. Christensen, Karen Hennum, Anikó Várnai, Edith Buchinger, Siri Grandal, Gaston Courtade, Olav A. Hegnar, Finn L. Aachmann, Vincent G. H. Eijsink

“…The enzyme, expressed in Escherichia coli, showed C4-oxidative cleavage of amorphous cellulose and soluble cello-oligosaccharides. Activity on xyloglucan, mixed-linkage β-glucan, and glucomannan was also observed, and product profiles differed compared to the well-studied C4-oxidizing NcLPMO9C from Neurospora crassa. …”
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The P.E.A.N.U.T. Method: Update on an Integrative System Approach for the Treatment of Chronic Otitis Media with Effusion and Adenoid Hypertrophy in Children by Henrik Szőke, Márta Maródi, Jan Vagedes, Balázs Székely, István Magyarosi, Adél Bedő, Veronika Fellegi, Krisztina Somogyvári, Péter Móricz

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Comparative characterization of all cellulosomal cellulases from Clostridium thermocellum reveals high diversity in endoglucanase product formation essential for complex activity by Benedikt Leis, Claudia Held, Fabian Bergkemper, Katharina Dennemarck, Robert Steinbauer, Alarich Reiter, Matthias Mechelke, Matthias Moerch, Sigrid Graubner, Wolfgang Liebl, Wolfgang H. Schwarz, Vladimir V. Zverlov

“…These modes are shown on amorphous cellulose and on model cello-oligosaccharides (with degree of polymerization DP 3 to 6). …”
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Wearer Experience and Eye Care Professional Acceptance with a 1 Day Multifocal Contact Lens by McParland M, Walsh K, Comoroda D, Sulley A

“…Professional Affairs, San Ramon, CA, USA; 2Lumanity (Formerly Cello Health Insight), Insight Department, Bethesda, MD, USA; 3Coopervision, Inc. …”
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Efficacy of early treatment with hydroxychloroquine in people with mild to moderate COVID-19: a systematic review and meta-analysis by Adrian V. Hernandez, John Ingemi III, Michael Sherman, Vinay Pasupuleti, Joshuan J. Barboza, Alejandro Piscoya, Yuani M. Roman, C. Michael White

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Heterologously Expressed Cellobiose Dehydrogenase Acts as Efficient Electron-Donor of Lytic Polysaccharide Monooxygenase for Cellulose Degradation in <i>Trichoderma reesei</i> by Muhammad Adnan, Xuekun Ma, Yanping Xie, Abdul Waheed, Gang Liu

“…<i>Pc</i>CDH has high cello-oligosaccharide k_cat, K_m, and k_cat/K_m values. …”
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Exploring the association of ESR1 and ESR2 gene SNPs with polycystic ovary syndrome in human females: a comprehensive association study by Fatima Muccee, Naeem Mahmood Ashraf, Suhail Razak, Tayyaba Afsar, Nadia Hussain, Fohad Mabood Husain, Huma Shafique

“…Methods For this study, SNPs in ESR1 and ESR2 genes were retrieved from the ENSEMBL database and analyzed for their effect on mutated proteins using different bioinformatics tools including SIFT, PolyPhen, CADD, REVEL, MetaLR, I-Mutant, CELLO2GO, ProtParam, SOPMA, SWISS-MODEL and HDDOCK. …”
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Recognition of a Novel Gene Signature for Human Glioblastoma by Chih-Hao Lu, Sung-Tai Wei, Jia-Jun Liu, Yu-Jen Chang, Yu-Feng Lin, Chin-Sheng Yu, Sunny Li-Yun Chang

“…Protein functional analysis by CELLO2GO implied that these signature genes might be involved in regulating various aspects of biological function, including anatomical structure development, cell proliferation and adhesion, signaling transduction and many of the genes were annotated in response to stress. …”
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Heterologous expression and characterization of novel GH12 β-glucanase and AA10 lytic polysaccharide monooxygenase from Streptomyces megaspores and their synergistic action in cell... by Xing Qin, Kun Yang, Jiahuan Zou, Xiaolu Wang, Tao Tu, Yuan Wang, Xiaoyun Su, Bin Yao, Huoqing Huang, Huiying Luo

“…Furthermore, the combination of SmBglu12A and SmLpmo10A enhanced enzymatic saccharification of phosphoric acid swollen cellulose by improving the native and oxidized cello-oligosaccharides yields. Conclusions These results proved for the first time that the AA10 LPMO was able to boost the catalytic efficiency of GH12 glycoside hydrolases on cellulosic substrates, providing another novel combination of glycoside hydrolase and LPMO for cellulose enzymatic saccharification.…”
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A highly efficient β-glucosidase from the buffalo rumen fungus <it>Neocallimastix patriciarum</it> W5 by Chen Hsin-Liang, Chen Yo-Chia, Lu Mei-Yeh, Chang Jui-Jen, Wang Hiaow-Ting, Ke Huei-Mien, Wang Tzi-Yuan, Ruan Sz-Kai, Wang Tao-Yuan, Hung Kuo-Yen, Cho Hsing-Yi, Lin Wan-Ting, Shih Ming-Che, Li Wen-Hsiung

“…Enzymatic assays showed that NpaBGS was active on short cello-oligosaccharides from various substrates. A weak activity in carboxymethyl cellulose (CMC) digestion indicated that the enzyme might also have the function of an endoglucanase. …”
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In-Silico Characterization of Estrogen Reactivating β-Glucuronidase Enzyme in GIT Associated Microbiota of Normal Human and Breast Cancer Patients by Fatima Muccee, Shakira Ghazanfar, Wajya Ajmal, Majid Al-Zahrani

“…Protein sequences of enzymes retrieved from UniProt database were subjected to ProtParam, CELLO2GO, SOPMA (secondary structure prediction method), PDBsum (Protein Database summaries), PHYRE2 (Protein Homology/AnalogY Recognition Engine), SAVES v6.0 (Structure Validation Server), MEME version 5.4.1 (Multiple Em for Motif Elicitation), Caver Web server v 1.1, Interproscan and Predicted Antigenic Peptides tool. …”
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In-Silico Investigation of Effects of Single-Nucleotide Polymorphisms in PCOS-Associated CYP11A1 Gene on Mutated Proteins by Fatima Muccee, Osama Bijou, Steve Harakeh, Rabi’atul Adawiyah, R. Z. Sayyed, Leila Haghshenas, Dikhnah Alshehri, Mohammad Javed Ansari, Shakira Ghazanfar

“…The protein sequence obtained was subjected to different tools including CELLO2GO, ProtParam, PHYRE2, I-Mutant, SIFT, and PolyPhen. …”
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Changes in active-site geometry on X-ray photoreduction of a lytic polysaccharide monooxygenase active-site copper and saccharide binding by Tobias Tandrup, Sebastian J. Muderspach, Sanchari Banerjee, Gianluca Santoni, Johan Ø. Ipsen, Cristina Hernández-Rollán, Morten H. H. Nørholm, Katja S. Johansen, Flora Meilleur, Leila Lo Leggio

“…Thus, the θ2 and θ3 parameters are helpful diagnostics for the oxidation state of the metal in a His-brace protein. On binding of cello-oligosaccharides to LsAA9_A, regardless of the production source, the θT parameter increases, making the Cu site less planar, while the active-site Tyr—Cu distance decreases reproducibly for the Cu2+ form. …”
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