Analysis of Flagellin-Specific Adaptive Immunity Reveals Links to Dysbiosis in Patients With Inflammatory Bowel DiseaseSummary by Laura Cook, Daniel J. Lisko, May Q. Wong, Rosa V. Garcia, Megan E. Himmel, Ernest G. Seidman, Brian Bressler, Megan K. Levings, Theodore S. Steiner

“…Methods: Blood was collected from 3 cohorts that included CD patients, UC patients, and healthy controls. Flow cytometry analyzed CD4+ T cells specific for Lachnospiraceae-derived A4-Fla2 and Escherichia coli H18 FliC flagellins, or control vaccine antigens. …”
Get full text

Function of miR-24 and miR-27 in Pediatric Patients With Idiopathic Nephrotic Syndrome by Fen-fen Ni, Guang-lei Liu, Shi-lei Jia, Ran-ran Chen, Li-bing Liu, Cheng-rong Li, Jun Yang, Xiao-Jie Gao

“…Purpose: We investigated the pathogenesis of idiopathic nephrotic syndrome (INS) by measuring the effects two specific miRNAs on Th2 cells in children with this disease.Methods: After informed consent, we enrolled 20 children with active INS before steroid initiation, 20 children with INS in remission after steroid therapy, and 20 age-matched healthy controls. Flow cytometry was used to measure the levels of Th2 cells and a cytometric bead array was used to measure the levels of IgE, interleukin (IL)−4, and IL-13. …”
Get full text

Cdan1 Is Essential for Primitive Erythropoiesis by Sharon Noy-Lotan, Sharon Noy-Lotan, Orly Dgany, Orly Dgany, Nathaly Marcoux, Nathaly Marcoux, Ayelet Atkins, Gary M. Kupfer, Linette Bosques, Christine Gottschalk, Orna Steinberg-Shemer, Orna Steinberg-Shemer, Orna Steinberg-Shemer, Benny Motro, Hannah Tamary, Hannah Tamary, Hannah Tamary, Hannah Tamary

“…Annexin V staining showed increases in both early and late-apoptotic erythroblasts compared to controls. Flow cytometry studies using the erythroid-specific cell-surface markers CD71 and Ter119 demonstrated that CdanΔEry erythroid progenitors do not undergo the semi-synchronous maturation characteristic of primitive erythroblasts. …”
Get full text

Leishmania infantum Defective in Lipophosphoglycan Biosynthesis Interferes With Activation of Human Neutrophils by Graziele Quintela-Carvalho, Graziele Quintela-Carvalho, Graziele Quintela-Carvalho, Astrid Madeleine Calero Goicochea, Astrid Madeleine Calero Goicochea, Vanessa Mançur-Santos, Vanessa Mançur-Santos, Sayonara de Melo Viana, Sayonara de Melo Viana, Yasmin da Silva Luz, Yasmin da Silva Luz, Beatriz Rocha Simões Dias, Beatriz Rocha Simões Dias, Milena Lázaro-Souza, Milena Lázaro-Souza, Martha Suarez, Martha Suarez, Camila Indiani de Oliveira, Camila Indiani de Oliveira, Elvira M. Saraiva, Cláudia I. Brodskyn, Cláudia I. Brodskyn, Patrícia T. Veras, Patrícia T. Veras, Juliana P.B. de Menezes, Juliana P.B. de Menezes, Bruno B. Andrade, Bruno B. Andrade, Bruno B. Andrade, Bruno B. Andrade, Bruno B. Andrade, Bruno B. Andrade, Jonilson Berlink Lima, Albert Descoteaux, Valéria M. Borges, Valéria M. Borges

“…Furthermore, collagen I matrix degradation was found to be significantly increased in ∆lpg1 parasite-infected cells but not in WT-infected controls. Flow cytometry analysis revealed a substantial boost in production of reactive oxygen species (ROS) during infection with either WT or ∆lpg1 L. infantum. …”
Get full text

Leukocyte-derived microparticles and scanning electron microscopic structures in two fractions of fresh cerebrospinal fluid in amyotrophic lateral sclerosis: a case report by Zachau Anne C, Landén Mikael, Mobarrez Fariborz, Nybom Rolf, Wallén Håkan, Wetterberg Lennart

“…However, scanning electron microscopy and fluorescence-activated cell sorting showed pronounced abnormalities compared to healthy controls. Flow cytometry analysis of two fractions of cerebrospinal fluid from our patient with amyotrophic lateral sclerosis was used to measure the specific binding of antibodies to CD42a, CD144 and CD45, and of phosphatidylserine to lactadherin. …”
Get full text

Platelet Function During Extracorporeal Membrane Oxygenation in Adult Patients by Camilla Mains Balle, Anni Nørgaard Jeppesen, Steffen Christensen, Anne-Mette Hvas, Anne-Mette Hvas

“…However, when taking platelet count into consideration, platelet aggregation relative to platelet count did not differ from healthy controls. Flow cytometry analyses demonstrated impaired platelet activation in ECMO patients on day 1 compared with healthy controls (all p < 0.03). …”
Get full text

Construction of software using gain-schedule/auto-adaptive control strategy for automation in drilling fluid production plants by S. C. Magalhães, G. Fontella, R. F. O. Borges, M. P. Vega, L. A. Calçada, C. M. Scheid

“…Preliminary tests were performed in a drilling fluid production unit, controlling flow rate through manipulation of the pump power engine. …”
Get full text

SOCS3 Regulates Dectin-2-Induced Inflammation in PBMCs of Diabetic Patients by Mohammed J. A. Haider, Zahraa Albaqsumi, Fahd Al-Mulla, Rasheed Ahmad, Fatema Al-Rashed

“…This study aims to evaluate the expression and function of Dectin-2 in peripheral blood mononuclear cells (PBMCs) isolated from diabetic patients and non-diabetic controls. Flow-cytometry and qRT-PCR were performed to evaluate the expression of Dectin-2 in different leukocyte subpopulations isolated from T2D patients (<i>n</i> = 10) and matched non-diabetic controls (<i>n</i> = 11). …”
Get full text

Holistic Security and Safety for Factories of the Future by Eva Maia, Sinan Wannous, Tiago Dias, Isabel Praça, Ana Faria

“…SMS-DT is represented in a three-tier architecture comprising the involved data and control flows: edge, platform, and enterprise tiers. …”
Get full text

Circulating Microvesicles in Convalescent Ischemic Stroke Patients: A Contributor to High-On-Treatment Residual Platelet Reactivity? by Diana Schrick, Tihamér Molnár, Margit Tőkés-Füzesi, Abigél Molnár, Erzsébet Ezer

“…Twenty age-matched healthy subjects served as controls. Flow cytometric measurements of microparicles (MVs) and data analysis were performed on Beckman-Coulter FC-500 cytometer with CXP software. …”
Get full text

Optimal operation and control of hybrid power systems with stochastic renewables and FACTS devices: An intelligent multi-objective optimization approach by Manoharan Premkumar, Tengku Juhana Tengku Hashim, Sowmya Ravichandran, Tan Ching Sin, Ramakrishnan Chandran, Anas R. Alsoud, Pradeep Jangir

Get full text

Microfluidic-based 3D cell culture for studies of biophysical and biochemical regulation of endothelial function by Vickerman, Vernella V. V. (Vernella Velonie Verlin)

Get full text

A Computational Pipeline for the Diagnosis of CVID Patients by Annelies Emmaneel, Annelies Emmaneel, Delfien J. Bogaert, Delfien J. Bogaert, Sofie Van Gassen, Sofie Van Gassen, Simon J. Tavernier, Simon J. Tavernier, Simon J. Tavernier, Melissa Dullaers, Filomeen Haerynck, Filomeen Haerynck, Yvan Saeys, Yvan Saeys

“…Using this pipeline, we analyzed the immunophenotypic profile in a pediatric and adult cohort of 28 patients with CVID, 23 patients with idiopathic primary hypogammaglobulinemia, 21 patients with IgG subclass deficiency, six patients with isolated IgA deficiency, one patient with isolated IgM deficiency, and 100 unrelated healthy controls. Flow cytometry analysis is traditionally done by manual identification of the cell populations of interest. …”
Get full text

A field development strategy for the joint optimization of flow allocations, well placements and well trajectories by M A Dada, M Mellal, A Makhloufi, H Belhouchet

“…Then, we used Matlab’s genetic algorithm toolbox to perform all the needed optimizations; from which, we were able to establish a drilling schedule for the set of wells to be realized, and we made it possible to simultaneously get the well location and configuration (vertical or horizontal), well type (producer or injector), well length, well orientation – in the horizontal plane –, as well as well controls (flow rates) and near wellbore pressure with respect to a set of linear and nonlinear-constraints. …”
Get full text

444 Deciphering the role of IL-4 in post-colitis repair by Nicolas F Moreno, Yang Yang, Jong-Min Jeong, Vivian Tran, Yankai Wen, Constance Atkins, Jie Zhao, Yuanyuan Fan, Junda Gao, Cynthia Ju

“…RESULTS/ANTICIPATED RESULTS: IL4-deficient mice had worse colitis compared with wild-type controls. Flow cytometry of lamina propria cells from 4get mice showed that most IL4-producing cells after colitis are eosinophils (CD11b+SiglecF+). …”
Get full text

Immiscible liquid-liquid displacement in microfluidic channels : effects of wettability and geometry by Lee, Hyundo

Get full text

Experimental visualization of the near-boundary hydrodynamics about fish-like swimming bodies by Techet, Alexandra Hughes

Get full text

Adaptable test bench for ASTM-compliant permeability measurement of porous scaffolds for tissue engineering by Stefano Gabetti, Beatrice Masante, Alessandro Schiavi, Elisa Scatena, Eleonora Zenobi, Simone Israel, Alessandro Sanginario, Costantino Del Gaudio, Alberto Audenino, Umberto Morbiducci, Diana Massai

“…In conclusion, the developed PTB and the proposed testing protocol allow the characterization of the intrinsic permeability of porous scaffolds of different types and dimensions under controlled flow regimes, representing a powerful tool in view of providing a reliable and repeatable framework for characterizing and optimizing scaffolds for TE applications.…”
Get full text

Relative humidity effect on the formation of highly oxidized molecules and new particles during monoterpene oxidation by X. Li, X. Li, S. Chee, J. Hao, J. P. D. Abbatt, J. Jiang, J. N. Smith

“…The ozonolysis of <span class="inline-formula"><i>α</i></span>-pinene, limonene, and <span class="inline-formula">Δ³</span>-carene, with and without OH scavengers, were carried out under low <span class="inline-formula">NO_<i>x</i></span> conditions under a range of RH (from <span class="inline-formula">∼3</span>&thinsp;% to <span class="inline-formula">∼92</span>&thinsp;%) in a temperature-controlled flow tube to generate secondary organic aerosol (SOA). …”
Get full text

Characterization of intrahepatic B cells in acute-on-chronic liver failure by Yudong Zhao, Wei He, Chenchen Wang, Nana Cui, Changjie Yang, Zhengrui You, Bisheng Shi, Lei Xia, Xiaosong Chen

“…Liver tissues were obtained from patients with ACLF, cirrhosis, and healthy controls. Flow cytometry was used to characterize the phenotypic and functional alterations in intrahepatic and circulating B-cell populations from ACLF, cirrhosis, and healthy controls. …”
Get full text