<i>Metschnikowia pulcherrima</i> and Related Pulcherrimin-Producing Yeasts: Fuzzy Species Boundaries and Complex Antimicrobial Antagonism by Matthias Sipiczki

“…The determination of the exact taxonomic position of the strains is hampered by the shortage of distinctive morphological and physiological properties of the species of the clade and the lack of rDNA barcode gaps. The rDNA repeats of the type strains of the species are not homogenized and are assumed to evolve by a birth-and-death mechanism combined with reticulation. …”
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The large Gunnera’s (G. tinctoria and G. manicata) in Europe in relation to EU regulation 1143/2014 by Johan L. C. H. van Valkenburg, Bruce A. Osborne, Marcel Westenberg

“…Given that it is often difficult to distinguish between these two large herbaceous species using morphological attributes we used standard chloroplast DNA barcode markers, supplemented at a later stage by ITS markers. …”
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Three cryptic Anaplecta (Blattodea, Blattoidea, Anaplectidae) species revealed by female genitalia, plus seven new species from China by Jing Zhu, Jiawei Zhang, Xinxing Luo, Zongqing Wang, Yanli Che

“…Morphological characteristics, including male and female genitalia, combined with DNA barcodes were used to identify 470 Anaplecta specimens sampled from China. …”
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Non-destructive collection and metabarcoding of arthropod environmental DNA remained on a terrestrial plant by Kinuyo Yoneya, Masayuki Ushio, Takeshi Miki

“…DNA is extracted from collected water and a DNA barcode region of cytochrome c oxidase subunit I (COI) gene is amplified and sequenced using a high-throughput Illumina Miseq platform. …”
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Revision of the Hawaiian psyllid genus Swezeyana, with descriptions of seven new species (Hemiptera, Psylloidea, Triozidae) by Diana M. Percy

“…Two species groups are represented by strikingly different female terminalia structure and endoskeletal development, although ovipositor structure is very similar between the two groups. Mitochondrial DNA barcodes (COI and cytB) are provided for eight of the nine species. …”
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A self‐propagating, barcoded transposon system for the dynamic rewiring of genomic networks by Max A English, Miguel A Alcantar, James J Collins

“…We then develop a modular, combinatorial assembly pipeline for the functionalization of transposons with synthetic or endogenous gene regulatory elements (e.g., inducible promoters) as well as DNA barcodes. We compare parallel evolutions across alternating carbon sources and demonstrate the emergence of inducible, multigenic phenotypes and the ease with which barcoded transposons can be tracked longitudinally to identify the causative rewiring of gene networks. …”
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High-throughput barcoding of nanoparticles identifies cationic, degradable lipid-like materials for mRNA delivery to the lungs in female preclinical models by Lulu Xue, Alex G. Hamilton, Gan Zhao, Zebin Xiao, Rakan El-Mayta, Xuexiang Han, Ningqiang Gong, Xinhong Xiong, Junchao Xu, Christian G. Figueroa-Espada, Sarah J. Shepherd, Alvin J. Mukalel, Mohamad-Gabriel Alameh, Jiaxi Cui, Karin Wang, Andrew E. Vaughan, Drew Weissman, Michael J. Mitchell

“…We then use barcoding technology to quantify how the selected 96 distinct lipid nanoparticles deliver DNA barcodes in vivo. The top-performing nanoparticle formulation delivering Cas9-based genetic editors exhibits therapeutic potential for antiangiogenic cancer therapy within a lung tumor model in female mice. …”
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Dissecting a complex chemical stress: chemogenomic profiling of plant hydrolysates by Jeffrey M Skerker, Dacia Leon, Morgan N Price, Jordan S Mar, Daniel R Tarjan, Kelly M Wetmore, Adam M Deutschbauer, Jason K Baumohl, Stefan Bauer, Ana B Ibáñez, Valerie D Mitchell, Cindy H Wu, Ping Hu, Terry Hazen, Adam P Arkin

“…Unfortunately, plant hydrolysates also contain many compounds that inhibit microbial growth and fermentation. We used DNA‐barcoded mutant libraries to identify genes that are important for hydrolysate tolerance in both Zymomonas mobilis (44 genes) and Saccharomyces cerevisiae (99 genes). …”
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Morphological and Phylogenetic Analyses Reveal a New Species of <i>Ceratocystiopsis</i> (<i>Ophiostomataceae, Ophiostomatales</i>) Associated with <i>Ips subelongatus</i> in Inner... by Zheng Wang, Ya Liu, Caixia Liu, Zhenyu Liu, Lijun Liang, Quan Lu

“…<i>Ceratocystiopsis</i> is a genus of the ophiostomatoid fungi in order <i>Ophiostomatales</i>. The shortage of DNA barcodes for many species in this genus has resulted in the presence of many unnamed cryptic species. …”
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Morphological and molecular characterization of Calicophoron raja (Näsmark, 1937) collected from wild Bovidae in South Africa by Aoi Ikeuchi, Daisuke Kondoh, Ali Halajian, Madoka Ichikawa-Seki

“…Therefore, the majority of the genetic information on paramphistomes found in the International Nucleotide Sequence Database is not supported by morphological descriptions, and the DNA barcodes of paramphistome species remain unreliable. …”
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VESPA: an optimized protocol for accurate metabarcoding-based characterization of vertebrate eukaryotic endosymbiont and parasite assemblages by Leah A. Owens, Sagan Friant, Bruno Martorelli Di Genova, Laura J. Knoll, Monica Contreras, Oscar Noya-Alarcon, Maria G. Dominguez-Bello, Tony L. Goldberg

“…Abstract Protocols for characterizing taxonomic assemblages by deep sequencing of short DNA barcode regions (metabarcoding) have revolutionized our understanding of microbial communities and are standardized for bacteria, archaea, and fungi. …”
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New records of Leptopilina, Ganaspis, and Asobara species associated with Drosophila suzukii in North America, including detections of L. japonica and G. brasiliensis by Paul K. Abram, Audrey E. McPherson, Robert Kula, Tracy Hueppelsheuser, Jason Thiessen, Steve J. Perlman, Caitlin I. Curtis, Jessica L. Fraser, Jordan Tam, Juli Carrillo, Michael Gates, Sonja Scheffer, Matthew Lewis, Matthew Buffington

“…Additionally, we found a species of Asobara associated with D. suzukii in British Columbia that is possibly Asobara rufescens (Förster) (known only from the Palearctic Region) based on COI DNA barcode data. These findings add to the list of cases documenting adventive establishment of candidate classical biological control agents outside of their native ranges. …”
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Molecular, morphological and acoustic identification of <i>Eumops maurus</i> and <i>Eumops hansae</i> (Chiroptera: Molossidae) with new reports from Central Amazonia by Adrià López-Baucells, Ricardo Rocha, Valéria Da Cunha Tavares, Ligiane Martins Moras, Sara Ema Silva, Paulo Estefano Dineli Bobrowiec, Christoph F.J. Meyer

“…Here, we describe records of these two molossids from the Central Brazilian Amazon, providing data on their external and craniodental morphology, DNA barcode (COI) sequences complemented by acoustic data for the species. …”
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Improved T cell receptor antigen pairing through data-driven filtering of sequencing information from single cells by Helle Rus Povlsen, Amalie Kai Bentzen, Mohammad Kadivar, Leon Eyrich Jessen, Sine Reker Hadrup, Morten Nielsen

“…Parallel capture of TCR transcripts and peptide-MHC is enabled through the use of reagents labeled with DNA barcodes. However, analysis and annotation of such single-cell sequencing (SCseq) data are challenged by dropout, random noise, and other technical artifacts that must be carefully handled in the downstream processing steps. …”
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The complete chloroplast genome sequence of Zingiber teres S. Q. Tong & Y. M. Xia (Zingiberaceae) by Xiao Wang, Shuming Tian, Hao Wang, Lin Yang, Xiaoling Zou, Xavier-ravi Baskaran, Qiang Li, Haitao Xing, Hong-Lei Li

“…The development of DNA barcodes could aid the identification of Zingiber species.…”
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Specific and Intraspecific Diversity of Symphypleona and Neelipleona (Hexapoda: Collembola) in Southern High Appalachia (USA) by Caroline D. Dukes, Frans Janssens, Ernesto Recuero, Michael S. Caterino

“…Due to poor representation in databases, and high intraspecific variability, no identifications were accomplished through comparison with available DNA barcodes.…”
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Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues by Saka, Sinem K, Wang, Yu, Kishi, Jocelyn Y, Zhu, Allen, Zeng, Yitian, Xie, Wenxin, Kirli, Koray, Yapp, Clarence, Cicconet, Marcelo, Beliveau, Brian J, Lapan, Sylvain W, Yin, Siyuan, Lin, Millicent, Boyden, Edward S, Kaeser, Pascal S, Pihan, German, Church, George M, Yin, Peng

“…Here we report immunostaining with signal amplification by exchange reaction (Immuno-SABER), which achieves highly multiplexed signal amplification via DNA-barcoded antibodies and orthogonal DNA concatemers generated by primer exchange reaction (PER). …”
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Molecular phylogeny of horseshoe crab using mitochondrial Cox1 gene as a benchmark sequence by John, Akbar, Khan Chowdhury, Ahmed Jalal, Yunus, Kamaruzzaman, Kasim, Zaleha, Kumar, C. Prasanna

“…An effort to assess the utility of 650 bp Cytochrome C oxidase subunit I (DNA barcode) gene in delineating the members horseshoe crabs (Family: xiphosura) with closely related sister taxa was made. …”
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A multiplex PCR method for rapid identification of commercially important seaweeds Kappaphycus alvarezii, Kappaphycus striatus and Eucheuma denticulatum (Rhodophyta, Solieriaceae) by Vun, Yee Thien, Thau, Wilson Lym Yong, Ann Anton, Grace Joy Wei Lie Chin

“…Taxonomic clarification of Kappaphycus alvarezii, Kappaphycus striatus and Eucheuma denticulatum based on standard DNA barcodes has proven to be useful as the phenotypic plasticity of the species can confound traditional taxonomic approaches. …”
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Application of Modern Approaches to Species Delimitation in Freshwater Erpobdellidae (Hirudinea: Arhynchobdellida) with a New Interpretation of Their Genera by Irina Kaygorodova

“…The use of molecular species delimitation methods made it possible to identify seven complexes of morphologically hidden (cryptic) species and some morphological misidentifications, as well as to discover a new species from Eastern Siberia (<i>Erpobdella sibirica</i>) with a unique molecular diagnosis (DNA barcode). A pioneering attempt to apply the same approach to higher taxa showed that Erpobdellidae probably consists of seven genera, including the unambiguous elimination of the genus <i>Motobdella</i>. …”
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