A Comprehensive Analysis of Chloroplast Genome Provides New Insights into the Evolution of the Genus <i>Chrysosplenium</i> by Tiange Yang, Zhihua Wu, Jun Tie, Rui Qin, Jiangqing Wang, Hong Liu

“…In addition, we identified 10 mutational hotspot regions that could serve as potential DNA barcodes for <i>Chrysosplenium</i> species identification. …”
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Comparative Analyses of Five Complete Chloroplast Genomes from the Genus <i>Pterocarpus</i> (Fabacaeae) by Zhou Hong, Zhiqiang Wu, Kunkun Zhao, Zengjiang Yang, Ningnan Zhang, Junyu Guo, Luke R. Tembrock, Daping Xu

“…In the five cp genomes of <i>Pterocarpus</i>, eight hypervariable regions, including trnH-GUG_psbA, trnS-UGA_psbC, accD-psaI, ndhI-exon2_ndhI-exon1, ndhG_ndhi-exon2, rpoC2-exon2, ccsA, and trnfM-CAU, are proposed for use as DNA barcode regions. In the comparison of gene selection pressures (<i>P. santalinus</i> as the reference genome), purifying selection was inferred as the primary mode of selection in maintaining important biological functions. …”
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Comparative Genomics and Phylogenetic Analysis of the Chloroplast Genomes in Three Medicinal <i>Salvia</i> Species for Bioexploration by Qing Du, Heyu Yang, Jing Zeng, Zhuoer Chen, Junchen Zhou, Sihui Sun, Bin Wang, Chang Liu

“…Two pairs of genus-specific DNA barcode primers were found. The results will provide a solid foundation to understand the phylogenetic classification of the three <i>Salvia</i> species. …”
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Biosystematic Study on Some Egyptian Species of <i>Astragalus</i> L. (Fabaceae) by Monier M. Abd El-Ghani, Ashraf S. A. El-Sayed, Ahmed Moubarak, Rabab Rashad, Hala Nosier, Adel Khattab

“…The current study includes the whole plant morphology, DNA barcode (ITS2), and molecular marker (SCoT). Ten taxa representing four species of <i>Astragalus</i> were collected from different localities in Egypt during the period from February 2018 to May 2019. …”
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Integrative taxonomy of Nearctic and Palaearctic Aleocharinae: new species, synonymies, and records (Coleoptera, Staphylinidae) by Adam J. Brunke, Mikko Pentinsaari, Jan Klimaszewski

“…Recently, integrated study of the two regions through detailed morphological comparisons and development of an authoritatively identified DNA barcode reference library has revealed the degree to which these two aleocharine faunas are interconnected, both naturally and through human activity. …”
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Phylogenomics and plastome evolution of Indigofera (Fabaceae) by Sheng-Mao Zhou, Fang Wang, Si-Yuan Yan, Zhang-Ming Zhu, Xin-Fen Gao, Xue-Li Zhao

“…Thirteen highly variable regions were identified, of which trnK-rbcL, ndhF-trnL, and ycf1 were considered as candidate DNA barcodes for species identification of Indigofera. …”
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Luminescent characteristics and mitochondrial COI barcodes of nine cohabitated Taiwanese fireflies by King-Siang Goh, Liang-Jong Wang, Jing-Han Ni, Tzi-Yuan Wang

“…The cytochrome oxidase I (COI) gene sequence was used as a DNA barcode to reveal the phylogenetic relationships of cohabitated species. …”
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Development of new tools and applications for high-throughput sequencing of microbiomes in environmental or clinical samples by Blackburn, Matthew Christopher

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Chloroplast genome assembly of Serjania erecta Raldk: comparative analysis reveals gene number variation and selection in protein-coding plastid genes of Sapindaceae by Leonardo C. J. Corvalán, Mariane B. Sobreiro, Larissa R. Carvalho, Renata O. Dias, Ramilla S. Braga-Ferreira, Ramilla S. Braga-Ferreira, Cintia P. Targueta, Carlos M. e Silva-Neto, Bianca W. Berton, Ana Maria S. Pereira, José A. F. Diniz-filho, Mariana P. C. Telles, Mariana P. C. Telles, Rhewter Nunes, Rhewter Nunes

“…We identified 10 and 11 mutational hotspots for Sapindaceae and Sapindoideae, respectively, and identified six highly diverse regions (tRNA-Lys — rps16, ndhC – tRNA-Val, petA – psbJ, ndhF, rpl32 – ccsA, and ycf1) are found in both groups, which show potential for the development of DNA barcode markers for molecular taxonomic identification of Serjania. …”
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Comparative Analysis and Characterization of Ten Complete Chloroplast Genomes of <i>Eremurus</i> Species (Asphodelaceae) by Dilmurod Makhmudjanov, Davlatali Abdullaev, Inom Juramurodov, Shakhzodbek Tuychiev, Ziyoviddin Yusupov, Hang Sun, Komiljon Tojibaev, Tao Deng

“…Among the protein-coding genes, five highly variable genes (<i>ycf1</i>, <i>rps15</i>, <i>rps16</i>, and <i>rpl36</i>) with high Pi values were detected and showed potential as DNA barcodes for the genus. Three genes (<i>rps19</i>, <i>ycf1</i>, and <i>ndhB</i>) had positive Ka/Ks values. …”
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Evolutionary and phylogenetic analyses of 11 Cerasus species based on the complete chloroplast genome by Tian Wan, Bai-xue Qiao, Jing Zhou, Ke-sen Shao, Liu-yi Pan, Feng An, Xu-sheng He, Tao Liu, Ping-ke Li, Yu-liang Cai

“…These results provide new insights into the plastome evolution of Cerasus, along with potential molecular markers and candidate DNA barcodes for further phylogenetic and phylogeographic analyses of Cerasus species.…”
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Genome-wide identification of colonisation determinants in rhizobium leguminosarum using BarSeq by Knights, H

“…Through incorporation of a random DNA barcode into transposons RB-TnSeq allows barcode sequencing (BarSeq), which can be used to assay mutant fitness in a high throughput manner. …”

ITS1 Barcode and Phytochemical Analysis by Gas Chromatography–Mass Spectrometry of <i>Corynaea crassa</i> Hook. f (Balanophoraceae) from Ecuador and Peru by Alexandra López-Barrera, Efrén Santos-Ordóñez, Ricardo Pacheco-Coello, Liliana Villao-Uzho, Migdalia Miranda, Yamilet Gutiérrez, Iván Chóez-Guaranda, Segundo Guillermo Ruiz-Reyes

“…Moreover, characterization of medicinal plants through the use of DNA barcodes is an important tool for phylogenetic analysis and identification of species; furthermore, complemented with phytochemical analysis, both are useful for identification of plant species and quality control of medicinal products. …”
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Analysis of Complete Chloroplast Genome: Structure, Phylogenetic Relationships of <i>Galega orientalis</i> and Evolutionary Inference of Galegeae by Junjie Feng, Yi Xiong, Xiaoli Su, Tianqi Liu, Yanli Xiong, Junming Zhao, Xiong Lei, Lijun Yan, Wenlong Gou, Xiao Ma

“…A comparative genomic analysis of 15 Galegeae species showed that most conserved non-coding sequence regions and two genic regions (<i>ycf1</i> and <i>clpP</i>) were highly divergent, which could be used as DNA barcodes for rapid and accurate species identification. …”
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Comparative Analysis of the Chloroplast Genomes of Eight Species of the Genus <i>Lirianthe</i> Spach with Its Generic Delimitation Implications by Tao Wu, Yong-Kang Sima, Shao-Yu Chen, Yu-Pin Fu, Hui-Fen Ma, Jia-Bo Hao, Yun-Feng Zhu

“…DNA polymorphism analysis revealed 10 highly variable regions located in the large single-copy and small single-copy regions, among which <i>rpl32-trnL</i>, <i>petA-psbJ</i>, and <i>trnH-psbA</i> were the recommended candidate DNA barcodes for the genus <i>Lirianthe</i> species. …”
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Complete Plastid Genome Sequencing of Eight Species from <i>Hansenia</i>, <i>Haplosphaera</i> and <i>Sinodielsia</i> (Apiaceae): Comparative Analyses and Phylogenetic Implications by Wei Gou, Sheng-Bin Jia, Megan Price, Xian-Lin Guo, Song-Dong Zhou, Xing-Jin He

“…The <i>ndhG-ndhI</i> spacer regions were found in all three genera to have higher diversity values (Pi values: 0.01028–0.2), and thus may provide potential DNA barcodes in phylogenetic analysis. IR boundary analysis showed that the length of <i>rps19</i> and <i>ycf1</i> genes entering IRs were usually stable in the same genus. …”
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Replacing mechanical protection with colorful faces–twice: parallel evolution of the non-operculate marine worm-snail genera Thylacodes (Guettard, 1770) and Cayo n. gen. (Gastropod... by Rüdiger Bieler, Timothy M. Collins, Rosemary Golding, Camila Granados-Cifuentes, John M. Healy, Timothy A. Rawlings, Petra Sierwald

“…A new polychromatic species, Thylacodes bermudensis n. sp., is described from Bermuda and compared morphologically and by DNA barcode markers to the likewise polychromatic western Atlantic species T. decussatus (Gmelin, 1791). …”
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Characterization of kaempferia species and activityguided selection of Kaempferia parviflora Wall. Ex baker for agronomic improvement by Labrooy, Catherine Dharshini

“…ITS 4 and 5 sequences are proposed as DNA barcodes for identification of Kaempferia species. …”
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Bionomics and host plants of the invasive Cydia interscindana (Möschler, 1866) (Lepidoptera, Tortricidae), an emerging pest in the Carpathian Lowlands by Attila Takács, Csaba Szabóky, Balázs Tóth, Miklós Bozsó, János Kutas, Szilárd Molnár, Gábor Farkas, Krisztina Erdélyi, Ilona Tunyoginé Búzás, Csaba Hargitai, Nikoletta Terman, Anna Menyhárt, Szabolcs Bodnár, Éva Gajdos, Sándor Bogya, Judit Csabai, Bianka Molnár, Antal Nagy

“…The damage caused by the larvae, the external morphology of the adult male, larva, pupa (described for the first time) and pupal exuviae are presented. We also analyse DNA barcodes, identifying this pest for the first time via DNA sequencing of immature stages. …”
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