Caffeine can override the S-M checkpoint in fission yeast. di Wang, S, Norbury, C, Harris, A, Toda, T

“...The replication checkpoint (or 'S-M checkpoint') control prevents progression into mitosis when DNA replication is incomplete. Caffeine has been known for some time to have the capacity to override the S-M checkpoint in animal cells. ...”

Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes. di Roodbarkelari, F, Bramsiepe, J, Weinl, C, Marquardt, S, Novák, B, Jakoby, M, Lechner, E, Genschik, P, Schnittger, A

“...A key question for the understanding of endoreplication cycles is how oscillating levels of cyclin-dependent kinase activity are generated that control repeated rounds of DNA replication. The APC/C performs a pivotal function in the mitotic cell cycle by promoting anaphase and paving the road for a new round of DNA replication. ...”

Analysis of Mcm2-7 chromatin binding during anaphase and in the transition to quiescence in fission yeast. di Namdar, M, Kearsey, S

“...Chromatin binding is lost in this transition in a process that does not require DNA replication or the selective degradation of specific subunits....”

Designing a new cross-linkable cohesin complex for studying cohesin`s interaction with DNA di Uluocak, P

“...Firstly, understanding the physical nature of cohesin’s interaction with chromatin before DNA replication is essential for determining how cohesion is established during DNA replication. ...”

Frequent exchange of the DNA polymerase during bacterial chromosome replication di Beattie, T, Kapadia, N, Nicolas, E, Uphoff, S, Wollman, A, Leake, M, Reyes-Lamothe, R

“...The replisome is a multiprotein machine that carries out DNA replication. In ...”

Understanding replication fork progression, stability, and chromosome fragility by exploiting the Suppressor of Underreplication protein di Nordman, Jared T., Orr-Weaver, Terry

“...There are many layers of regulation governing DNA replication to ensure that genetic information is accurately transmitted from mother cell to daughter cell. ...”
Testo
Testo

HAC stability in murine cells is influenced by nuclear localization and chromatin organization di Moralli, D, Chan, D, Jefferson, A, Volpi, E, Monaco, Z

“...The chromocenter acts as a hub for the deposition of heterochromatic markers, controlling centromeric and pericentromeric DNA replication timing and chromosome segregation. The HAC which localized more frequently outside the chromocenters bound variable amounts of histone H3 tri-methylated at lysine 9, and the high level of intraclonal variability was associated with an increase in HAC segregation errors and delayed DNA replication timing....”

Correction of proliferation and drug sensitivity defects in the progeroid Werner's Syndrome by Holliday junction resolution. di Rodriguez-Lopez, A, Whitby, M, Borer, C, Bachler, M, Cox, L

“...We have created isogenic WS cell lines expressing a nuclear-targeted bacterial Holliday junction endonuclease, RusA, and show that Holliday junction resolution by RusA restores DNA replication capacity in primary WS fibroblasts and enhances their proliferation. ...”

Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding. di Gregan, J, Lindner, K, Brimage, L, Franklin, R, Namdar, M, Hart, E, Aves, S, Kearsey, S

“...On return to growth, Cdc23 chromatin binding is rapidly reestablished independently from pre-RC formation, suggesting that chromatin association of Cdc23 provides a link between proliferation and competence to execute DNA replication....”

Chromosome replication dynamics in the archaeon Sulfolobus acidocaldarius. di Duggin, I, McCallum, SA, Bell, S

“...However, oriC2 was activated slightly later on average compared with oriC1 and oriC3. The DNA replication forks moved bidirectionally away from each origin at approximately 88 bp per second in synchronous culture. ...”

Nuclear distribution and chromatin association of DNA polymerase α-primase is affected by TEV protease cleavage of Cdc23 (Mcm10) in fission yeast di Yang, X, Gregan, J, Lindner, K, Young, H, Kearsey, S

“...Background: Cdc23/Mcm10 is required for the initiation and elongation steps of DNA replication but its biochemical function is unclear. ...”

E2F1 proteolysis via SCF-cyclin F underlies synthetic lethality between cyclin F loss and Chk1 inhibition di Burdova, K, Yang, H, Faedda, R, Hume, S, Chauhan, J, Ebner, D, Kessler, B, Vendrell, I, Drewry, D, Wells, C, Hatch, S, Dianov, G, Buffa, F, D'Angiolella, V

“...Chk1 inhibition in cells lacking cyclin F leads to DNA replication catastrophe. Replication catastrophe depends on accumulation of the transcription factor E2F1 in cyclin F-depleted cells. ...”

Transcription initiation activity sets replication origin efficiency in mammalian cells. di Sequeira-Mendes, J, Diaz-Uriarte, R, Apedaile, A, Huntley, D, Brockdorff, N, Gomez, M

“...Genomic mapping of DNA replication origins (ORIs) in mammals provides a powerful means for understanding the regulatory complexity of our genome. ...”

Investigations of the inhibition mechanisms of human ribonucleotide reductase by gemcitabine-5'-diphosphate and saccharomyces cerevisiae ribonucleotide reductase by Sml1 di Wang, Jun, Ph. D. Massachusetts Institute of Technology

Testo

A microscopic study of cohesin di Rhodes, J

“...</p> <p><b>Cohesin can remain associated with chromosomes during DNA replication</b> – Sister chromatid cohesion is established at the DNA replication fork. ...”

The examination of mechanisms underlying meiotic chromosome segregation di Brar, Gloria Ann

Testo

Hypomorphic PCNA mutation underlies a human DNA repair disorder di Baple, E, Chambers, H, Cross, H, Fawcett, H, Nakazawa, Y, Chioza, B, Harlalka, G, Mansour, S, Sreekantan-Nair, A, Patton, M, Muggenthaler, M, Rich, P, Wagner, K, Coblentz, R, Stein, C, Last, J, Taylor, A, Jackson, A, Ogi, T, Lehmann, A, Green, C, Crosby, A

“...PCNA is a highly conserved sliding clamp protein essential for DNA replication and repair. Due to this fundamental role, mutations in PCNA that profoundly impair protein function would be incompatible with life. ...”

MRG15-mediated tethering of PALB2 to unperturbed chromatin protects active genes from genotoxic stress di Bleuyard, J, Fournier, M, Nakato, R, Couturier, A, Katou, Y, Ralf, C, Hester, S, Dominguez, D, Rhodes, D, Humphrey, T, Shirahige, K, Esashi, F

“...Missense mutations that ablate PALB2 binding to MRG15 confer elevated sensitivity to the topoisomerase inhibitor camptothecin (CPT) and increased levels of aberrant metaphase chromosomes and DNA stress in gene bodies, which were suppressed by preventing DNA replication. Remarkably, the level of PALB2 at genic regions was frequently decreased, rather than increased, upon CPT treatment. ...”

Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding di Gregan, J, Lindner, K, Brimage, L, Franklin, R, Namdar, M, Hart, E, Aves, S, Kearsey, S

“...On return to growth, Cdc23 chromatin binding is rapidly reestablished independently from pre-RC formation, suggesting that chromatin association of Cdc23 provides a link between proliferation and competence to execute DNA replication....”

Nuclear distribution and chromatin association of DNA polymerase α-primase is affected by TEV protease cleavage of Cdc23 (Mcm10) in fission yeast di Yang, X, Gregan, J, Lindner, K, Young, H, Kearsey, S

“... <p style="text-align:justify;"> <b>Background:</b> Cdc23/Mcm10 is required for the initiation and elongation steps of DNA replication but its biochemical function is unclear. ...”