Crystal structure of human pirin: an iron-binding nuclear protein and transcription cofactor. von Pang, H, Bartlam, M, Zeng, Q, Miyatake, H, Hisano, T, Miki, K, Wong, L, Gao, G, Rao, Z

“… These findings suggest an enzymatic role for Pirin, most likely in biological redox reactions involving oxygen, and provide compelling evidence that Pirin requires the participation of the metal ion for its interaction with Bcl-3 to co-regulate the NF-kappaB transcription pathway and the interaction with NFI in DNA replication. Substitution of iron by heavy metals thus provides a novel pathway for these metals to directly influence gene transcription.  …”

Hypoxia-induced SETX links replication stress with the unfolded protein response von Ramachandran, S, Ma, T, Griffin, J, Ng, N, Foskolou, I, Hwang, M-S, Victori, P, Cheng, W-C, Buffa, F, Leszczynska, K, El-Khamisy, S, Gromak, N, Hammond, E

“… In the absence of hypoxia-induced SETX, R-loop levels increase, DNA damage accumulates, and DNA replication rates decrease. Therefore, suggesting that, SETX plays a key role in protecting cells from DNA damage induced during transcription in hypoxia.  …”

4D Visualization of replication foci in mammalian cells corresponding to individual replicons von Chagin, V, Casas-Delucchi, C, Reinhart, MR, Schermelleh, L, Markaki, Y, Maiser, A, Bolius, J, Bensimon, AB, Filies, M, Domaing, P, Rozanov, Y, Leonhardt, H, Cardoso, M

“… Since the pioneering proposal of the replicon model of DNA replication 50 years ago, the predicted replicons have not been identified and quantified at the cellular level.  …”

The vertebrate Hef ortholog is a component of the Fanconi anemia tumor-suppressor pathway. von Mosedale, G, Niedzwiedz, W, Alpi, A, Perrina, F, Pereira-Leal, J, Johnson, M, Langevin, F, Pace, P, Patel, K

“… Notably, Hef interacts directly with DNA structures that are intermediates in DNA replication. This discovery sheds light on the origins, regulation and molecular function of the FA tumor-suppressor pathway in the maintenance of genome stability. …”

Characterisation of the interaction between WRN, the helicase/exonuclease defective in progeroid Werner's syndrome, and an essential replication factor, PCNA. von Rodríguez-López, A, Jackson, D, Nehlin, J, Iborra, F, Warren, A, Cox, L

“… We demonstrate co-localisation of WRN and PCNA at replication factories, show that PCNA binds to two distinct functional sites on WRN, and suggest a mechanism by which association between WRN and PCNA may be regulated in cells on DNA damage and during DNA replication. …”

Cyclin E ablation in the mouse. von Geng, Y, Yu, Q, Sicinska, E, Das, M, Schneider, J, Bhattacharya, S, Rideout, WM, Bronson, RT, Gardner, H, Sicinski, P

“… Molecular analyses revealed that cells lacking cyclin E fail to normally incorporate MCM proteins into DNA replication origins during G(0)-->S progression.  …”

Interplay between Fanconi anemia and homologous recombination pathways in genome integrity von Michl, J, Zimmer, J, Tarsounas, M

“… Here, we focus on the clinical and mechanistic implications of the connection between these two cancer susceptibility syndromes and on how these two molecular pathways of DNA replication and repair interact functionally to prevent genomic instability. …”

A yeast model for the study of human DFNA5, a gene mutated in nonsyndromic hearing impairment. von Gregan, J, Van Laer, L, Lieto, L, Van Camp, G, Kearsey, S

“… We identified new rat and horse DFNA5 homologues and we describe a domain of homology shared between DFNA5 and the Mcm10 family of DNA replication proteins. Genetic interactions between heterologously expressed DFNA5 and a fission yeast cdc23 (mcm10) mutant support a possible link between DFNA5 and Mcm10 proteins. …”

Azadirachtin disrupts formation of organised microtubule arrays during microgametogenesis of Plasmodium berghei. von Billker, O, Shaw, M, Jones, I, Ley, S, Mordue, A, Sinden, R

“… Male gametogenesis involves three rounds of DNA replication and endomitosis, and the assembly de novo of 8 motile axonemes.  …”

Replicative DNA polymerase mutations in cancer von Heitzer, E, Tomlinson, I

“… Three DNA polymerases - Pol α, Pol δ and Pol e{open} - are essential for DNA replication. After initiation of DNA synthesis by Pol α, Pol δ or Pol e{open} take over on the lagging and leading strand respectively.  …”

DUBbing cancer: Deubiquitylating enzymes involved in epigenetics, DNA damage and the cell cycle as therapeutic targets von Pinto-Fernández, A, Kessler, B

“… A number of critical checkpoints ascertain progression through the different stages of the cell cycle, which can be aborted when perturbed, for instance by errors in DNA replication and repair. These molecular checkpoints are regulated by a number of proteins that need to be present at the right time and quantity.  …”

Interlinked bistable mechanisms generate robust mitotic transitions von Hutter, L, Rata, S, Hochegger, H, Novak, B

“… The transitions between phases of the cell cycle have evolved to be robust and switch-like, which ensures temporal separation of DNA replication, sister chromatid separation, and cell division.  …”

ATRX dysfunction induces replication defects in primary mouse cells von Clynes, D, Jelinska, C, Xella, B, Ayyub, H, Taylor, S, Mitson, M, Bachrati, C, Higgs, D, Gibbons, R

“… Using a mouse knockout model we demonstrate that ATRX plays a direct role in facilitating DNA replication. Ablation of ATRX alone, although leading to a DNA damage response at telomeres, is not sufficient to trigger the alternative lengthening of telomere pathway in mouse embryonic stem cells. …”

Coordination of multiple enzyme activities by a single PCNA in archaeal Okazaki fragment maturation. von Beattie, T, Bell, S

“… Chromosomal DNA replication requires one daughter strand-the lagging strand-to be synthesised as a series of discontinuous, RNA-primed Okazaki fragments, which must subsequently be matured into a single covalent DNA strand.  …”

Regulation of monoubiquitinated PCNA by DUB autocleavage. von Huang, T, Nijman, S, Mirchandani, K, Galardy, P, Cohn, M, Haas, W, Gygi, S, Ploegh, H, Bernards, R, D'Andrea, A

“… Here, we show that the DUB ubiquitin specific protease 1 (USP1) deubiquitinates the DNA replication processivity factor, PCNA, as a safeguard against error-prone translesion synthesis (TLS) of DNA.  …”

Conditional inactivation of replication proteins in fission yeast using hormone-binding domains von Pai, C, Schnick, J, MacNeill, SA, Kearsey, SE

“… The fission yeast Schizosaccharomyces pombe is a useful model for analysing DNA replication as genetic methods to allow conditional inactivation of relevant proteins can provide important information about S-phase execution.  …”

Genome stability during cell proliferation: A systems analysis of the molecular mechanisms controlling progression through the eukaryotic cell cycle von Novák, B, Heldt, F, Tyson, J

“… Well-nourished cells in a favorable environment (well-supplied with growth factors, cytokines, and/or hormones and free from stresses, ionizing radiation, etc.) will grow, replicate their genome, and divide into two daughter cells, fully prepared to repeat the process. This cycle of DNA replication and division underlies all aspects of biological growth, reproduction, repair and development.  …”

Simultaneous visualization of FISH signals and bromo-deoxyuridine incorporation by formamide-free DNA denaturation. von Moralli, D, Monaco, Z

“… In this chapter, we present a technique, which allows identification of specific DNA sequences by fluorescence in situ hybridization (FISH) and simultaneously analyses the incorporation of a thymidine analogue, 5-bromo-2-deoxyuridine (BrdU), to mark DNA replication. First, tissue culture cells are synchronized at the beginning of the S-phase.  …”

Multiple SP1 binding sites confer enhancer-independent, replication-activated transcription of HIV-1 and globin gene promoters. von Proudfoot, N, Lee, B, Monks, J

“… This activation process can be achieved either by DNA replication of the promoter-containing plasmid or by high concentrations of input plasmid DNA used in the transfections.  …”

Biomolecular Condensates in the Nucleus von Sabari, Benjamin R, Dall’Agnese, Alessandra, Young, Richard A

“… © 2020 Elsevier Ltd Nuclear processes such as DNA replication, transcription, and RNA processing each depend on the concerted action of many different protein and RNA molecules.  …”
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