Solid-state NMR studies of chromatin structure and dynamics by Prasanna, Chinmayi

“…Understanding the dynamic organization of chromatin is indispensable as it provides crucial information to delineate cellular processes that it mediates–DNA replication, transcription, and repair. Nucleosome core particle (NCP) composed of core histones, organizing ~147 bp of DNA constitutes the fundamental unit of eukaryotic chromatin. …”
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Structural biology of proteins involved in the replication of herpesviruses by Hew, Kelly Kai Li

“…Production of new infectious herpesvirus particles is dependent on the disruptions of host immune responses, herpesvirus DNA replication, assembly and reactivation from latency. …”
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Pengaruh polimorfisme C677T gena methylentetrahydrofolate reductase (MTHFR) terhadap kejadian relaps pada anak dengan Leukemia limfoblastik akut (LLA) by , DENNY WELLYAM SIGARLAKI, , Dr. dr. Sri Mulatsih, SpAK

“…Methotrexate is a key drug in acute lymphoblastic leukemia (ALL) treatment. It inhibits DNA replication by blocking the conversion of 5,10 methylenetetrahydrofolate to 5-methylenetetrahydrofolate by methylene tetrahydrofolate reductase, a critical enzyme involved in folate metabolism, DNA methylation and synthesis. …”

Coordination of structure-specific nucleases by human SLX4/BTBD12 is required for DNA repair. by Muñoz, I, Hain, K, Déclais, A, Gardiner, M, Toh, G, Sanchez-Pulido, L, Heuckmann, J, Toth, R, Macartney, T, Eppink, B, Kanaar, R, Ponting, C, Lilley, D, Rouse, J

“…Budding yeast Slx4 interacts with the structure-specific endonuclease Slx1 to ensure completion of ribosomal DNA replication. Slx4 also interacts with the Rad1-Rad10 endonuclease to control cleavage of 3' flaps during repair of double-strand breaks (DSBs). …”

The PCNA unloader Elg1 promotes recombination at collapsed replication forks in fission yeast by Tamang, S, Kishkevich, A, Morrow, C, Osman, F, Jalan, M, Whitby, M

“…Protein-DNA complexes can impede DNA replication and cause replication fork collapse. Whilst it is known that homologous recombination is deployed in such instances to restart replication, it is unclear how a stalled fork transitions into a collapsed fork at which recombination proteins can load. …”

Orchestrating the nucleases involved in DNA interstrand cross-link (ICL) repair. by Sengerová, B, Wang, A, McHugh, P

“…In mammalian cells, much ICL repair occurs in association with DNA replication during S phase, following the stalling of a replication fork at the block caused by an ICL lesion. …”

The role of the DNA sliding clamp in Okazaki fragment maturation in archaea and eukaryotes. by Beattie, T, Bell, S

“…Efficient processing of Okazaki fragments generated during discontinuous lagging-strand DNA replication is critical for the maintenance of genome integrity. …”

Identification of an MCM4 homologue expressed specifically in the sexual stage of Plasmodium falciparum. by Li, J, Cox, L

“…Mini-chromosome maintenance (MCM) proteins play an essential role in DNA replication initiation. We have isolated a novel gene encoding an MCM-like protein from the human malaria parasite Plasmodium falciparum using the vectorette technique. …”

MUS81 nuclease activity is essential for replication stress tolerance and chromosome segregation in BRCA2-deficient cells by Lai, X, Broderick, R, Bergoglio, V, Zimmer, J, Badie, S, Niedzwiedz, W, Hoffmann, J, Tarsounas, M

“…Here we investigate whether MUS81 could similarly facilitate DNA replication in the context of BRCA2 abrogation. Our results demonstrate that replication fork progression in BRCA2-deficient cells requires MUS81. …”

Cohesin phosphorylation and mobility of SMC1 at ionizing radiation-induced DNA double-strand breaks in human cells. by Bauerschmidt, C, Woodcock, M, Stevens, D, Hill, M, Rothkamm, K, Helleday, T

“…Cohesin, a hetero-tetrameric complex of SMC1, SMC3, Rad21 and Scc3, associates with chromatin after mitosis and holds sister chromatids together following DNA replication. Following DNA damage, cohesin accumulates at and promotes the repair of DNA double-strand breaks. …”

RAD51 localization and activation following DNA damage. by Tarsounas, M, Davies, A, West, S

“…By contrast, RAD51 foci that form spontaneously as cells undergo DNA replication at S phase occur without the need for BRCA2 or the RAD51 paralogues. …”

Structure of an aprataxin-DNA complex with insights into AOA1 neurodegenerative disease. by Tumbale, P, Appel, C, Kraehenbuehl, R, Robertson, P, Williams, J, Krahn, J, Ahel, I, Williams, R

“…DNA ligases finalize DNA replication and repair through DNA nick-sealing reactions that can abort to generate cytotoxic 5'-adenylation DNA damage. …”

R‐loop formation during S phase is restricted by PrimPol‐mediated repriming by Šviković, S, Crisp, A, Tan-Wong, S, Guilliam, T, Doherty, A, Proudfoot, N, Guilbaud, G, Sale, J

“…During DNA replication, conflicts with ongoing transcription are frequent and require careful management to avoid genetic instability. …”

Identification and characterization of a Drosophila ortholog of WRN exonuclease that is required to maintain genome integrity. by Saunders, R, Boubriak, I, Clancy, D, Cox, L

“…WRN is implicated in restraining illegitimate recombination, especially during DNA replication. Here we identify a Drosophila ortholog of the WRN exonuclease encoded by the CG7670 locus. …”

Monopolar attachment of sister kinetochores at meiosis I requires casein kinase 1. by Petronczki, M, Matos, J, Mori, S, Gregan, J, Bogdanova, A, Schwickart, M, Mechtler, K, Shirahige, K, Zachariae, W, Nasmyth, K

“…In meiosis, a single round of DNA replication is followed by two consecutive rounds of chromosome segregation, called meiosis I and II. …”

Specialized replication mechanisms maintain genome stability at human centromeres by Scelfo, A, Angrisani, A, Grillo, M, Barnes, BM, Muyas, F, Sauer, CM, Leung, CWB, Dumont, M, Grison, M, Mazaud, D, Garnier, M, Guintini, L, Nelson, L, Esashi, F, Cortés-Ciriano, I, Taylor, SS, Déjardin, J, Wilhelm, T, Fachinetti, D

“…Locus-specific proteomics identifies specialized DNA replication and repair proteins at centromeres, highlighting them as difficult-to-replicate regions. …”

Time-keeping and decision-making in the cell cycle by Tyson, JJ, Novak, B

“…Cell growth, DNA replication, mitosis and division are the fundamental processes by which life is passed on from one generation of eukaryotic cells to the next. …”

Capturing the dynamics of genome replication on individual ultra-long nanopore sequence reads by Müller, C, Boemo, M, Spingardi, P, Kessler, B, Kriaucionis, S, Simpson, J, Nieduszynski, C

“…We used sequencing reads of 20–160 kilobases to generate a whole-genome single-molecule map of DNA replication dynamics and discover a class of low-frequency stochastic origins in budding yeast. …”

Meiotic prophase requires proteolysis of M phase regulators mediated by the meiosis-specific APC/CAma1. by Okaz, E, Argüello-Miranda, O, Bogdanova, A, Vinod, P, Lipp, J, Markova, Z, Zagoriy, I, Novak, B, Zachariae, W

“…Whereas proliferating cells enter M phase shortly after DNA replication, the first M phase of meiosis is preceded by an extended prophase in which homologous chromosomes undergo recombination. …”

A model of yeast cell-cycle regulation based on multisite phosphorylation. by Barik, D, Baumann, W, Paul, MR, Novak, B, Tyson, J

“…In order for the cell's genome to be passed intact from one generation to the next, the events of the cell cycle (DNA replication, mitosis, cell division) must be executed in the correct order, despite the considerable molecular noise inherent in any protein-based regulatory system residing in the small confines of a eukaryotic cell. …”