Recapitulation of Werner syndrome sensitivity to camptothecin by limited knockdown of the WRN helicase/exonuclease by Bird, J, Jennert-Burston, K, Bachler, M, Mason, P, Lowe, J, Heo, S, Campisi, J, Faragher, R, Cox, L

“…WRN is a RecQ helicase with an associated exonuclease activity important in DNA metabolism, including DNA replication, repair and recombination. In humans, deficiencies in WRN function cause the segmental progeroid Werner syndrome (WS), in which patients show premature onset of many hallmarks of normal human ageing. …”

Splitting the nucleus: What's wrong with the tripartite ring model? by Nasmyth, K, Oliveira, R

“…How sister DNAs enter the cohesin ring and are retained inside for long periods of time after the completion of DNA replication remains poorly understood. © 2010 Cold Spring Harbor Laboratory Press.…”

Replication fork blockage by transcription factor-DNA complexes in Escherichia coli. by Payne, B, van Knippenberg, I, Bell, H, Filipe, SR, Sherratt, D, McGlynn, P

“…All organisms require mechanisms that resuscitate replication forks when they break down, reflecting the complex intracellular environments within which DNA replication occurs. Here we show that as few as three lac repressor-operator complexes block Escherichia coli replication forks in vitro regardless of the topological state of the DNA. …”

DNA copy-number measurement of genome replication dynamics by high-throughput sequencing: the sort-seq, sync-seq and MFA-seq family. by Batrakou, DG, Müller, CA, Wilson, RHC, Nieduszynski, CA

“…Genome replication follows a defined temporal programme that can change during cellular differentiation and disease onset. DNA replication results in an increase in DNA copy number that can be measured by high-throughput sequencing. …”

Dancing around the divisome: asymmetric chromosome segregation in Escherichia coli. by Wang, X, Possoz, C, Sherratt, D

“…By simultaneously tracking pairs of specific genetic regions and divisome proteins in live Escherichia coli, we develop a new scheme for the relationship between DNA replication-segregation, chromosome organization, and cell division. …”

Homologous recombination suppresses transgenerational DNA end resection and chromosomal instability in fission yeast by Pai, C-C, Durley, SC, Cheng, W-C, Chiang, N-Y, Peters, J, Kasparek, T, Blaikley, E, Wee, B-Y, Walker, C, Kearsey, SE, Buffa, F, Murray, JM, Humphrey, TC

“…Inherited chromosomes carrying a single-ended DSB are subject to cycles of DNA replication and extensive end-processing across successive cell divisions. …”

Rec8 phosphorylation by casein kinase 1 and Cdc7-Dbf4 kinase regulates cohesin cleavage by separase during meiosis. by Katis, V, Lipp, J, Imre, R, Bogdanova, A, Okaz, E, Habermann, B, Mechtler, K, Nasmyth, K, Zachariae, W

“…During meiosis, two rounds of chromosome segregation after a single round of DNA replication produce haploid gametes from diploid precursors. …”

The antitumorigenic roles of BRCA1–BARD1 in DNA repair and replication by Tarsounas, M, Sung, P

“…The tumour suppressor breast cancer type 1 susceptibility protein (BRCA1) promotes DNA double-strand break (DSB) repair by homologous recombination and protects DNA replication forks from attrition. BRCA1 partners with BRCA1-associated RING domain protein 1 (BARD1) and other tumour suppressor proteins to mediate the initial nucleolytic resection of DNA lesions and the recruitment and regulation of the recombinase RAD51. …”

A three-in-one-bullet for oesophageal cancer: replication fork collapse, spindle attachment failure and enhanced radiosensitivity generated by a ruthenium(II) metallo-intercalator by Gill, MR, Jarman, PJ, Halder, S, Walker, MG, Saeed, HK, Thomas, JA, Smythe, C, Ramadan, K, Vallis, KA

“…These results establish that DNA replication is the target for [Ru(phen) 2 (tpphz)] 2+ and provide the first experimental evidence that ruthenium-based intercalation targets multiple genome integrity pathways in cancer cells, thereby achieving enhanced selectivity compared to existing DNA-damaging agents such as cisplatin.…”

Minimal models for cell-cycle control based on competitive inhibition and multisite phosphorylations of Cdk substrates. by Gérard, C, Tyson, J, Novák, B

“…A modest level of Cdk activity is sufficient to initiate DNA replication, but mitosis and APC activation require an elevated Cdk activity. …”

The HelQ human DNA repair helicase utilizes a PWI-like domain for DNA loading through interaction with RPA, triggering DNA unwinding by the HelQ helicase core by Jenkins, T, Northall, SJ, Ptchelkine, D, McHugh, P, Et al.

“…HelQ helicase is a component of human DNA maintenance systems that prevent or reverse genome instability arising during DNA replication. Here, we provide details of the molecular mechanisms that underpin HelQ function—its recruitment onto ssDNA through interaction with replication protein A (RPA), and subsequent translocation of HelQ along ssDNA. …”

Mutational meltdown of putative microbial altruists in Streptomyces coelicolor colonies by Zhang, Z, Shitut, S, Claushuis, B, Claessen, D, Rozen, DE

“…In addition, the base-substitution rate in mutants increases roughly 10-fold, possibly due to mutations in genes for DNA replication and repair. Ecological damage, caused by reduced sporulation, coupled with DNA damage due to point mutations and deletions, leads to an inevitable and irreversible type of mutational meltdown in these cells. …”

The Mendelian colorectal cancer syndromes by Tomlinson, I

“…Major functional pathways are consistently inactivated in the Mendelian CRC syndromes: certain types of DNA repair (proofreading of DNA replication errors, mismatch repair and base excision repair) and signalling (bone morphogenetic protein (BMP), Wnt signalling and mTOR). …”

Splitting the nucleus: what's wrong with the tripartite ring model? by Nasmyth, K, Oliveira, R

“…How sister DNAs enter the cohesin ring and are retained inside for long periods of time after the completion of DNA replication remains poorly understood.…”

A semi-synthetic neolignan derivative from dihydrodieugenol B selectively affects the bioenergetic system of Leishmania infantum and inhibits cell division by Amaral, M, de Sousa, F, Silva, T, Junior, A, Taniwaki, N, Johns, D, Lago, J, Anderson, E, Tempone, A

“…The derivative also interfered with the cell cycle of Leishmania, inducing a programmed cell death-like mechanism and affecting DNA replication. Further immunomodulatory studies demonstrated that the compound eliminates amastigotes via an independent activation of the host cell, with decrease levels of IL-10, TNF and MCP-1. …”

New approaches to understanding the spatial organization of bacterial genomes by Le, Tung, Laub, Michael T

“…However, chromosomes cannot be haphazardly packed, and instead must adopt structures compatible with numerous cellular processes, including DNA replication, chromosome segregation, recombination, and gene expression. …”
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Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice by Kojima, Mina, de Rooij, Dirk G., Page, David C

“…STRA8 binds its own promoter and those of thousands of other genes, including meiotic prophase genes, factors mediating DNA replication and the G1-S cell-cycle transition, and genes that promote the lengthy prophase unique to meiosis I. …”
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Ribonucleotide Reductases: Structure, Chemistry, and Metabolism Suggest New Therapeutic Targets by Greene, Brandon L., Kang, Gyunghoon, Cui, Chang, Bennati, Marina, Norcera, Daniel G., Drennan, Catherine L, Stubbe, JoAnne

“…In doing so, they play an important role in fidelity of DNA replication and repair. RNRscentral role in nucleic acid metabolism has resulted in five therapeutics that inhibit human RNRs. …”
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Multiscale modeling of genome organization with maximum entropy optimization by Lin, Xingcheng, Qi, Yifeng, Latham, Andrew P, Zhang, Bin

“…Three-dimensional (3D) organization of the human genome plays an essential role in all DNA-templated processes, including gene transcription, gene regulation, and DNA replication. Computational modeling can be an effective way of building high-resolution genome structures and improving our understanding of these molecular processes. …”
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Regulation of chromatin interactions to the MYC oncogene by long non-coding RNA PVT1 in acute myeloid leukemia cell lines by Nur Zahwah Asa'ad Sameer Bagharib

“…Chromatin interactions have been found to play pivotal roles in important cellular events such as the regulation of gene expression, cell division and DNA replication. Dysregulated chromatin interactions can therefore have significant effects on the cell and promote diseases such as cancer. …”
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