DNA choreography: correlating mobility and organization of DNA across different resolutions from loops to chromosomes 由 Pabba, MK, Meyer, J, Celikay, K, Schermelleh, L, Rohr, K, Cardoso, MC

“...Here, we made use of DNA replication to directly label genomic DNA in an unbiased genome-wide manner. ...”

Allele loss on chromosome 11q and microsatellite instability in malignant melanoma. 由 Tomlinson, I, Beck, N, Bodmer, W

“...However, no mutations of the mismatch repair genes hMSH2 and hMLH1 were detected in these two tumours, and the observed replication errors may result from mutations in other genes involved in mismatch repair or DNA replication. LOH on 11q and replication errors appear to comprise part of the genetic pathways of several tumour types, including melanomas....”

Characterisation of cytoplasmic uridyl transferases in yeast and human cells 由 Schmidt, M

“...Results showed that ZCCHC11 is required for efficient destabilisation of histone mRNAs following inhibition or completion of DNA replication. In agreement with this finding, cDNA sequencing experiments showed that ZCCHC11-mediated uridylation is particularly prevalent at the end of S phase. ...”

Fluorescence in situ hybridization applications for super-resolution 3D structured illumination microscopy. 由 Markaki, Y, Smeets, D, Cremer, M, Schermelleh, L

“...We describe typical problems encountered with super-resolution imaging of in situ hybridizations in mammalian tissue culture cells and provide optimized DNA-/(RNA)-FISH protocols including combinations with immunofluorescence staining (Immuno-FISH) and DNA replication labeling using click chemistry....”

The nuclear matrix protein CIZ1 facilitates localisation of Xist RNA to the inactive X-chromosome territory 由 Ridings-Figueroa, R, Stewart, E, Nesterova, T, Coker, H, Pintacuda, G, Godwin, J, Wilson, R, Haslam, A, Lilley, F, Ruigrok, R, Bageghni, S, Albadrani, G, Mansfield, W, Roulson, J, Brockdorff, N, Ainscough, J, Coverley, D

“...The nuclear matrix protein Cip1-interacting zinc finger protein 1 (CIZ1) promotes DNA replication in association with cyclins, and has been linked with adult and pediatric cancers. ...”

Bacterial Chromosome Organization and Segregation 由 Badrinarayanan, Anjana, Le, Tung, Laub, Michael T

“...Not only must cells massively compact their genetic material, but they must also organize their DNA in a manner that is compatible with a range of cellular processes, including DNA replication, DNA repair, homologous recombination, and horizontal gene transfer. ...”
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The response of Plasmodium falciparum to isoleucine withdrawal is dependent on the stage of progression through the intraerythrocytic cell cycle 由 McLean, Kyle J., Jacobs-Lorena, Marcelo

“...Conclusions: The markedly different response of the parasite to isoleucine withdrawal before or after the onset of DNA replication is reminiscent of the nutrient-dependent G1 cell cycle checkpoints described in other organisms....”
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DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7 由 De Jesús-Kim, Lorraine, Friedman, Larry J, Lõoke, Marko, Ramsoomair, Christian K, Gelles, Jeff, Bell, Stephen P

“...The committed step of eukaryotic DNA replication occurs when the pairs of Mcm2-7 replicative helicases that license each replication origin are activated. ...”
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Mechanistic Model for Production of Recombinant Adeno-associated Virus via Triple Transfection of HEK293 Cells 由 Nguyen, Tam NT, Sha, Sha, Hong, Moo Sun, Maloney, Andrew J, Barone, Paul W, Neufeld, Caleb, Wolfrum, Jacqueline, Springs, Stacy L, Sinskey, Anthony J, Braatz, Richard D

“...Sensitivity analysis suggests that (1) the poorly coordinated timeline of capsid synthesis and viral DNA replication results in a low ratio of full virions in harvest, and (2) repressive function of the Rep protein could be impeding capsid production at a later phase. ...”
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BE.440 Analysis of Biological Networks, Fall 2004 由 Essigmann, John, Sasisekharan, Ram

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Dynamics of two phosphorelays controlling cell cycle progression in 1 Caulobacter crescentus 由 Chen, Y. Erin, Tsokos, Christos G., Biondi, Emanuele G., Perchuk, Barrett, Laub, Michael T

“...By identifying mutations that uncouple these two activities, we show that CckA's phosphatase activity is important for downregulating CtrA prior to DNA replication initiation in vivo but that other phosphatases may exist. ...”
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Investigating the communication of mismatch recognition and strand incision in the DNA mismatch repair pathway 由 Banaz, N

“...DNA Mismatch Repair (MMR) is crucial for preventing DNA replication errors that escape the proofreading mechanisms of DNA polymerases from becoming permanent mutations. ...”

Small molecule inhibitors uncover synthetic genetic interactions of human flap endonuclease 1 (FEN1) with DNA damage response genes 由 Ward, T, McHugh, P, Durant, S

“...Flap endonuclease 1 (FEN1) is a structure selective endonuclease required for proficient DNA replication and the repair of DNA damage. Cellularly active inhibitors of this enzyme have previously been shown to induce a DNA damage response and, ultimately, cell death. ...”

Simian virus 40 large T-antigen G-quadruplex DNA helicase inhibition by G-quadruplex DNA-interactive agents. 由 Tuesuwan, B, Kern, J, Thomas, P, Rodriguez, M, Li, J, David, WM, Kerwin, S

“...On the basis of growing evidence for G-quadruplex DNA structures in genomic DNA and the presumed need to resolve these structures for DNA replication, the G-quadruplex DNA unwinding ability of a prototypical replicative helicase, SV40 large T-antigen (T-ag), was investigated. ...”

Rif1 acts through Protein Phosphatase 1 but independent of replication timing to suppress telomere extension in budding yeast 由 Kedziora, S, Gali, V, Wilson, R, Clark, K, Nieduszynski, C, Hiraga, S, Donaldson, A

“...Rif1 was recently shown to control DNA replication by acting as a Protein Phosphatase 1 (PP1)-targeting subunit. ...”

CHK1 inhibition exacerbates replication stress induced by IGF blockade 由 Wu, X, Seraia, E, Hatch, SB, Wan, X, Ebner, DV, Aroldi, F, Jiang, Y, Ryan, AJ, Bogenrieder, T, Weyer-Czernilofsky, U, Rieunier, G, Macaulay, VM

“...We recently reported that genetic or pharmacological inhibition of insulin-like growth factor receptor (IGF-1R) slows DNA replication and induces replication stress by downregulating the regulatory subunit RRM2 of ribonucleotide reductase, perturbing deoxynucleotide triphosphate (dNTP) supply. ...”

Two bistable switches govern M phase entry 由 Mochida, S, Rata, S, Hino, H, Nagai, T, Novák, B

“...The abrupt and irreversible transition from interphase to M phase is essential to separate DNA replication from chromosome segregation. This transition requires the switch-like phosphorylation of hundreds of proteins by the cyclin-dependent kinase 1 (Cdk1):cyclin B (CycB) complex. ...”

BRCA2 loss induces an innate immune response via activation of the cGAS-STING pathway 由 Reisländer, T

“...As an early response to loss of BRCA2 (4 days), genes involved in cell cycle, organelle fission, chromosome segregation, DNA replication and DNA repair were downregulated. Furthermore, cell cycle progression was perturbed which was associated with cell cycle arrest in G1. ...”

DNA polymerase ε and δ exonuclease domain mutations in endometrial cancer. 由 Church, D, Briggs, SE, Palles, C, Domingo, E, Kearsey, S, Grimes, J, Gorman, M, Martin, L, Howarth, K, Hodgson, S, NSECG Collaborators, Kaur, K, Taylor, J, Tomlinson, I

“...The high fidelity of eukaryotic DNA replication is due to a combination of accurate incorporation of nucleotides into the nascent DNA strand by DNA polymerases, the recognition and removal of mispaired nucleotides (proofreading) by the exonuclease activity of DNA polymerases δ and ε, and post-replication surveillance and repair of newly synthesized DNA by the mismatch repair (MMR) apparatus. ...”

Transcriptomic analysis of Rhizobium leguminosarum bacteroids in determinate and indeterminate nodules 由 Green, RT, East, A, Karunakaran, R, Downie, JA, Poole, PS

“...Pea bacteroids had increased expression of genes associated with DNA replication, membrane synthesis and the TCA (tricarboxylic acid) cycle. ...”