Generation of Transfer-DNA-Free Base-Edited Citrus Plants by Berta Alquézar, Berta Alquézar, Stefania Bennici, Lourdes Carmona, Alessandra Gentile, Leandro Peña, Leandro Peña

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Effect of a Mating Type Gene Editing in <i>Lentinula edodes</i> Using RNP/Nanoparticle Complex by Minseek Kim, Minji Oh, Ji-Hoon Im, Eun-Ji Lee, Hojin Ryu, Hyeon-Su Ro, Youn-Lee Oh

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DNA-free gene editing in plants: a brief overview by Tsveta Tsanova, Lidia Stefanova, Lora Topalova, Atanas Atanasov, Ivelin Pantchev

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Editorial: Methods and applications of CRISPR technology in plant sciences, 2022 by Ljudmilla Timofejeva, Davinder Singh

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Establishment of a PEG-mediated protoplast transformation system based on DNA and CRISPR/Cas9 ribonucleoprotein complexes for banana by Shaoping Wu, Haocheng Zhu, Jinxing Liu, Qiaosong Yang, Xiuhong Shao, Fangcheng Bi, Chunhua Hu, Heqiang Huo, Kunling Chen, Ganjun Yi

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Defining Optimized Properties of Modified mRNA to Enhance Virus- and DNA- Independent Protein Expression in Adult Stem Cells and Fibroblasts by Frauke Hausburg, Silke Na, Natalia Voronina, Anna Skorska, Paula Müller, Gustav Steinhoff, Robert David

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A streamlined guide RNA screening system for genome editing in Sorghum bicolor by Jeong Sun Lee, Su-Ji Bae, Jin-Soo Kim, Changsoo Kim, Beum-Chang Kang

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Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application by Jin-Jun Yue, Jin-Ling Yuan, Fu-Hui Wu, Yu-Hsuan Yuan, Qiao-Wei Cheng, Chen-Tran Hsu, Choun-Sea Lin

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Optimization of highly efficient exogenous-DNA-free Cas9-ribonucleoprotein mediated gene editing in disease susceptibility loci in wheat (Triticum aestivum L.) by Snigdha Poddar, Snigdha Poddar, Jaclyn Tanaka, Katherine L. D. Running, Gayan K. Kariyawasam, Justin D. Faris, Timothy L. Friesen, Myeong-Je Cho, Jamie H. D. Cate, Jamie H. D. Cate, Jamie H. D. Cate, Brian Staskawicz, Brian Staskawicz

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Molecular and Computational Strategies to Increase the Efficiency of CRISPR-Based Techniques by Lucia Mattiello, Mark Rütgers, Maria Fernanda Sua-Rojas, Rafael Tavares, José Sérgio Soares, Kevin Begcy, Marcelo Menossi

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The Free Energy of Nucleosomal DNA Based on the Landau Model and Topology by Huimin Yang, Xuguang Shi

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Current insights and advances into plant male sterility: new precision breeding technology based on genome editing applications by Silvia Farinati, Samela Draga, Angelo Betto, Fabio Palumbo, Alessandro Vannozzi, Margherita Lucchin, Gianni Barcaccia

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Comparison of an automated DNA extraction and 16S rDNA real time PCR/sequencing diagnostic method using optimized reagents with culture during a 15-month study using specimens from... by Konrad Egli, Martin Risch, Lorenz Risch, Thomas Bodmer

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Knockout of BnaX.SGT.a caused significant sinapine reduction in transgene-free rapeseed mutants generated by protoplast-based CRISPR RNP editing by Oliver Moss, Xueyuan Li, Eu Sheng Wang, Selvaraju Kanagarajan, Rui Guan, Emelie Ivarson, Li-Hua Zhu

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