Visualization of c-di-GMP in multicellular Dictyostelium stages by Hayato Ide, Yukihisa Hayashida, Yusuke V. Morimoto, Yusuke V. Morimoto

“…The bacterial signaling molecule cyclic diguanosine monophosphate (c-di-GMP) is only synthesized and utilized by the cellular slime mold Dictyostelium discoideum among eukaryotes. Dictyostelium cells undergo a transition from a unicellular to a multicellular state, ultimately forming a stalk and spores. …”
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PTEN hopping on the cell membrane is regulated via a positively-charged C2 domain. by Masato Yasui, Satomi Matsuoka, Masahiro Ueda

“…Here we show that positively charged residues located in the cα2 helix of the C2 domain are necessary for the membrane localization of PTEN via stable electrostatic interactions in Dictyostelium discoideum. Single-molecule imaging analyses revealed that PTEN molecules moved distances much larger than expected had they been caused by lateral diffusion, a phenomenon we call "hopping." …”
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Dictyostelium: An Important Source of Structural and Functional Diversity in Drug Discovery by Yuzuru Kubohara, Haruhisa Kikuchi

“…The cellular slime mold Dictyostelium discoideum is an excellent model organism for the study of cell and developmental biology because of its simple life cycle and ease of use. …”
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Evidence for different mechanisms of chloroquine resistance in 2 Plasmodium species that cause human malaria. by Nomura, T, Carlton, J, Baird, J, del Portillo, H, Fryauff, D, Rathore, D, Fidock, D, Su, X, Collins, W, McCutchan, T, Wootton, J, Wellems, T

“…This report describes pfcrt homologues from P. vivax, P. knowlesi, P. berghei, and Dictyostelium discoideum. Synteny between the P. falciparum and P. vivax genes is demonstrated. …”

Dictyostelium as a model to assess site-specific ADP-ribosylation events by Kolb, A, Hsu, D, Wallis, A, Ura, S, Rakhimova, A, Pears, C, Lakin, N

“…The amoeba Dictyostelium discoideum is a single-cell organism that can undergo a simple developmental program, making it an excellent model to study the molecular mechanisms of cell motility, signal transduction, and cell-type differentiation. …”

PARP regulates nonhomologous end joining through retention of Ku at double-strand breaks. by Couto, C, Wang, H, Green, J, Kiely, R, Siddaway, R, Borer, C, Pears, C, Lakin, N

“…In this paper, we exploit Dictyostelium discoideum to uncover a novel role for PARylation in regulating nonhomologous end joining (NHEJ). …”

PARP regulates nonhomologous end joining through retention of Ku at double-strand breaks by Couto, C, Wang, H, Green, J, Kiely, R, Siddaway, R, Borer, C, Pears, C, Lakin, N

“…In this paper, we exploit Dictyostelium discoideum to uncover a novel role for PARylation in regulating nonhomologous end joining (NHEJ). …”

Xpf suppresses the mutagenic consequences of phagocytosis in Dictyostelium by Pontel, LB, Langenick, J, Rosado, IV, Zhang, X, Traynor, D, Kay, RR, Patel, KJ

“…Here, we describe an unusual genetic interaction between DNA repair in the unicellular amoeba Dictyostelium discoideum and its natural bacterial food source. …”

Cells at the center of dictyostelium aggregates become spores. by Huang, H, Takagawa, D, Weeks, G, Pears, C

“…The cellular slime mold <em>Dictyostelium discoideum</em> undergoes a developmental life cycle on starvation to generate a fruiting body consisting of a mass of spores supported on a stalk of dead, vacuolated cells. …”

Plasticity of cell migration resulting from mechanochemical coupling by Yuansheng Cao, Elisabeth Ghabache, Wouter-Jan Rappel

“…The model predictions are explicitly verified by systematically reducing the protrusive force of the actin network in experiments using Dictyostelium discoideum cells. Our results indicate the importance of coupling signaling events to cell mechanics and morphology and may be applicable in a wide variety of cell motility systems.…”
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Replacement of the essential <it>Dictyostelium </it>Arp2 gene by its <it>Entamoeba </it>homologue using parasexual genetics by Fütterer Klaus, King Jason, Zaki Mehreen, Insall Robert H

“…</p> <p>Results</p> <p>We have established a genetic strategy that uses parasexual genetics to allow essential <it>Dictyostelium discoideum </it>genes to be manipulated and replaced with modified or tagged homologues. …”
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Dictyostelium cells migrate similarly on surfaces of varying chemical composition. by Colin P McCann, Erin C Rericha, Chenlu Wang, Wolfgang Losert, Carole A Parent

“…We asked whether mechanotransduction also occurs during non-integrin mediated migration by examining the motion of the social amoeba Dictyostelium discoideum, which is thought to bind non-specifically to surfaces. …”
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Dictyostelium as a model to assess site-specific ADP-ribosylation events by Kolb, A-L, Hsu, D-W, Wallis, ABA, Ura, S, Rakhimova, A, Pears, CJ, Lakin, ND

“…The amoeba Dictyostelium discoideum is a single-cell organism that can undergo a simple developmental program, making it an excellent model to study the molecular mechanisms of cell motility, signal transduction, and cell-type differentiation. …”

Dictyostelium as a model to assess site-specific ADP-ribosylation events by Kolb, A-L, Hsu, D-W, Wallis, ABA, Ura, S, Rakhimova, A, Pears, CJ, Lakin, ND

“…The amoeba Dictyostelium discoideum is a single-cell organism that can undergo a simple developmental program, making it an excellent model to study the molecular mechanisms of cell motility, signal transduction, and cell-type differentiation. …”

Potassium Sensitive Optical Nanosensors Containing Voltage Sensitive Dyes by Xiaojiang Xie, Agustín Gutiérrez, Valentin Trofimov, Istvan Szilagyi, Thierry Soldati, Eric Bakker

“…The nanosensors were readily delivered into the social amoeba Dictyostelium discoideum in a non-invasive manner, forming a promising new platform for intracellular ion quantification and imaging. …”
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The Use of Diffusion Calculations and Monte Carlo Simulations to Understand the Behavior of Cells in Dictyostelium Communities by Richard H. Gomer

“…For instance, the eukaryotic microbe Dictyostelium discoideum proliferates as single cells, and when starved, the cells aggregate together and form structures of ~20,000 cells. …”
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Simple to Complex: The Role of Actin and Microtubules in Mitochondrial Dynamics in Amoeba, Yeast, and Mammalian Cells by Meghan D. Jones, Kari Naylor

“…In this review, we focus on microtubules and actin filaments and look at what is currently known about the cytoskeleton’s role in mitochondrial dynamics in complex models like mammals and yeast, as well as what is known in the simple model system, <i>Dictyostelium discoideum</i>. Understanding how the cytoskeleton is involved in mitochondrial dynamics increases our understanding of mitochondrial disease, especially neurodegenerative diseases. …”
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CRISPR/Cas9-based genome-wide screening of Dictyostelium by Takanori Ogasawara, Jun Watanabe, Remi Adachi, Yusuke Ono, Yoichiro Kamimura, Tetsuya Muramoto

“…The simple eukaryote Dictyostelium discoideum has a unique life cycle and is often used as a crucial research model for a wide range of biological processes and rare metabolites. …”
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A homologue of Cdk8 is required for spore cell differentiation in Dictyostelium. by Lin, H, Khosla, M, Huang, H, Hsu, D, Michaelis, C, Weeks, G, Pears, C

“…Here, we report that a Cdk8 homologue from Dictyostelium discoideum is localized in the nucleus where it forms part of a high molecular weight complex that has CTD kinase activity. …”

mTORC1 pathway activity biases cell fate choice by Wang, Y, Papayova, M, Warren, E, Pears, CJ

“…The social amoeba Dictyostelium discoideum is a simplified system to study choice preference as proliferating amoebae enter a developmental cycle upon starvation and differentiate into two major cell types, stalk and spores, organised in a multicellular fruiting body. …”