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    Cryopreservation of vegetative cells and zygotes of the multicellular volvocine green alga Gonium pectorale by Hisayoshi Nozaki, Fumi Mori, Yoko Tanaka, Ryo Matsuzaki, Haruyo Yamaguchi, Masanobu Kawachi

    Published 2022-04-01
    “…In total, ten cryopreserved strains of G. pectorale were newly established in the Microbial Culture Collection at the National Institute for Environmental Studies. Although the cryopreservation of zygotes of volvocine algae has not been previously reported, high rates (approximately 60%) of G. pectorale zygote germination were observed after thawing zygotes that had been cryopreserved with 5% or 10% methanol as the cryoprotectant during two-step cooling and freezing in liquid nitrogen. …”
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    Environmental Science & its Components / by Flournoy, Corinne, author 640242

    Published 2012
    “…Related areas of study include environmental studies and environmental engineering. Environmental studies incorporates more of the social sciences for understanding human relationships, perceptions and policies towards the environment. …”
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