Interferometric excitation fluorescence lifetime imaging microscopy by Pavel Malý, Dita Strachotová, Aleš Holoubek, Petr Heřman

“…Abstract Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. …”
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Introduction to the Special Issue on Fluorescence Lifetime Imaging Microscopy (FLIM) by Junle Qu, Liwei Liu

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Rapid global fitting of large fluorescence lifetime imaging microscopy datasets. by Sean C Warren, Anca Margineanu, Dominic Alibhai, Douglas J Kelly, Clifford Talbot, Yuriy Alexandrov, Ian Munro, Matilda Katan, Chris Dunsby, Paul M W French

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Rapid fluorescence lifetime imaging microscopy via few-photon imaging by Ming-Jie Sun, Yi-Cheng Zhang, Fang-Rui Lin, Shuai Wang, Li-Wei Liu, Jun-Le Qu

“…Conventional fluorescence lifetime imaging microscopy (FLIM) based on time-correlated single photon counting has great potential in various domains, notably in cellular biology, enabling comprehensive studies encompassing spatiotemporal dynamics and quantitative analysis of fluorescence lifetimes. …”
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Monitoring conformational changes of proteins in cells by fluorescence lifetime imaging microscopy. by Calleja, V, Ameer-Beg, S, Vojnovic, B, Woscholski, R, Downward, J, Larijani, B

“…Any conformational changes will cause a variation in FRET, which, in turn, is detected by fluorescence lifetime imaging microscopy ("FLIM"). Protein kinase B (PKB)/Akt, a key component of phosphoinositide 3-kinase-mediated signalling, was selected for this purpose. …”

Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy by Maojia Huang, Xinyue Liang, Zixiao Zhang, Jing Wang, Yiyan Fei, Jiong Ma, Songnan Qu, Lan Mi

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Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy by Chao Liu, Xinwei Wang, Yan Zhou, Yuliang Liu

“…Time-gated fluorescence lifetime imaging microscopy (FLIM) can provide high resolution and high imaging frame during mature FLIM methods. …”
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Fluorescence Lifetime Imaging Microscopy of Porphyrins in <i>Helicobacter pylori</i> Biofilms by Antonella Battisti, Paola Morici, Antonella Sgarbossa

“…The knowledge of the photophysical characteristics of Hp porphyrins in their physiological biofilm microenvironment is crucial to implement and optimize the photodynamic treatment. Fluorescence lifetime imaging microscopy (FLIM) of intrinsic bacterial porphyrins was performed and data were analyzed by the ‘fit-free’ phasor approach in order to map the distribution of the different fluorescent species within Hp biofilm. …”
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Improving fluorescence lifetime imaging microscopy phasor accuracy using convolutional neural networks by Varun Mannam, Jacob P. Brandt, Cody J. Smith, Xiaotong Yuan, Scott Howard

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Biopolymer hydroxyapatite composite materials: Adding fluorescence lifetime imaging microscopy to the characterization toolkit by Quinn T. Easter

“…Fluorescence microscopy is one powerful tool for investigating materials on these length scales. Fluorescence lifetime imaging microscopy (FLIM), a technique that measures the fluorescence lifetime of fluorophores based on local environments, could be used to observe the structural properties of materials. …”
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Visualizing orthogonal RNAs simultaneously in live mammalian cells by fluorescence lifetime imaging microscopy (FLIM) by Nadia Sarfraz, Emilia Moscoso, Therese Oertel, Harrison J. Lee, Suman Ranjit, Esther Braselmann

“…Here, the authors show that fluorescence lifetime imaging microscopy is advantageous for multiplexed RNA visualization while achieving robust cellular contrast.…”
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Insights into Metabolic Activity and Structure of the Retina through Multiphoton Fluorescence Lifetime Imaging Microscopy in Mice by Niranjana Kesavamoorthy, Jason A. Junge, Scott E. Fraser, Hossein Ameri

Subjects: “…multiphoton fluorescence lifetime imaging microscopy…”
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Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation by Daniëlle Rianne José Verboogen, Natalia González Mancha, Martin ter Beest, Geert van den Bogaart

“…We developed a novel Förster resonance energy transfer-fluorescence lifetime imaging microscopy (FRET-FLIM)-based technique allowing visualization of real-time local interactions of fluorescently tagged SNARE proteins in live cells. …”
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An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy by Lanigan, P, Dunsby, C, McGinty, J, Elson, D, Requejo-Isidro, J, Munro, I, Galletly, N, Treanor, B, Önfelt, B, McCann, F, Davis, D, Neil, M, French, P

An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy by Dunsby, C, Lanigan, P, McGinty, J, Elson, D, Requejo-Isidro, J, Munro, I, Galletly, N, McCann, F, Treanor, B, Onfelt, B, Davis, D, Neil, M, French, P

An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy by Dunsby, C, Lanigan, P, McGinty, J, Elson, D, Requejo-Isidro, J, Munro, I, Galletly, N, McCann, F, Treanor, B, Önfelt, B, Davis, D, Neil, M, French, P

An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy by Lanigan, P, Dunsby, C, McGinty, J, Elson, D, Requejo-Isidro, J, Munro, I, Galletly, N, Treanor, B, Onfelt, B, McCann, F, Davis, D, Neil, M, French, P, IEEE

Dynamics of HIV-1 Gag Processing as Revealed by Fluorescence Lifetime Imaging Microscopy and Single Virus Tracking by Chen Qian, Annica Flemming, Barbara Müller, Don C. Lamb

“…Here, fluorescence lifetime imaging microscopy and single virus tracking are used to follow Gag processing in nascent HIV-1 particles in situ. …”
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Fluorescence lifetime imaging microscopy (FLIM): a non-traditional approach to study host-microbial symbioses by Elizabeth Hinde, Linda L. Blackall, Madeleine J. H. van Oppen, Douglas R. Brumley, Sarah Jane Tsang Min Ching, Iromi Wanigasuriya, Pranali Deore

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A robust method for autofluorescence-free immunofluorescence using high-speed fluorescence lifetime imaging microscopy by Wonsang Hwang, Tyler McPartland, Sinyoung Jeong, Conor L. Evans

“…While various techniques, including chemical- and quencher-based methods, photobleaching, and digital-based protocols like autofluorescence image subtraction, have been developed to suppress autofluorescence, each approach has inherent limitations that restrict their efficacy across different tissue types. Fluorescence lifetime imaging microscopy (FLIM) offers a powerful alternative by leveraging the distinct lifetime-spectrum profiles of fluorophores to differentiate specific immunofluorescence signals from autofluorescence. …”
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