Accurate identification of Culicidae at aquatic developmental stages by MALDI-TOF MS profiling by Constentin Dieme, Amina Yssouf, Anubis Vega-Rúa, Jean-Michel Berenger, Anna-Bella Failloux, Didier Raoult, Philippe Parola, Lionel Almeras

“…The database included a total of 95 laboratory-reared specimens of 6 mosquito species, including Anopheles gambiae (S form), Anopheles coluzzi (M form), Culex pipiens pipiens, Culex pipiens molestus, Aedes aegypti and 2 colonies of Aedes albopictus. …”
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Natural Anophelin mosquito repellents and phytochemical analysis of ethanol and hexane leaf extracts from four plant species by Emad I. Y. Adam, Yousif O.H.M. Assad, Marwa M. E. Eltohami, Nabil H.H. Bashir, Samira H. Abdelrahman, Bakri Y. M. Nour

“…OH extracts, C. forskohlii exhibited higher repellency potential on Anopheles gambiae with protection time of 137.3 min, while O. basilicum, E. camaldulensis and Cy. flexuosus registered protection time of 30.6, 15.3 and 19 min, respectively. …”
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The impact of residual infections on Anopheles-transmitted Wuchereria bancrofti after multiple rounds of mass drug administration by Dziedzom K. de Souza, Rashid Ansumana, Santigie Sessay, Abu Conteh, Benjamin Koudou, Maria P. Rebollo, Joseph Koroma, Daniel A. Boakye, Moses J. Bockarie

“…Results The results indicated the presence of MF infected children in Pujehun district. An. gambiae collected in the district were also positive for W. bancrofti, even though the prevalence of infection was below the threshold associated with active transmission. …”
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A closer look at the WHO cone bioassay: video analysis of the hidden effects of a human host on mosquito behaviour and insecticide contact by Angela Hughes, Agnes Matope, Mischa Emery, Keith Steen, Gregory Murray, Hilary Ranson, Philip J. McCall, Geraldine M. Foster

“…Four strains of Anopheles gambiae (insecticide susceptible: Kisumu and N’gousso; insecticide resistant: Banfora and VK7) were tested with and without the host attractant using untreated, Permanet 2.0 and Olyset nets. …”
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Rapid classification of epidemiologically relevant age categories of the malaria vector, Anopheles funestus by Emmanuel P. Mwanga, Doreen J. Siria, Issa H. Mshani, Sophia H. Mwinyi, Said Abbasi, Mario Gonzalez Jimenez, Klaas Wynne, Francesco Baldini, Simon A. Babayan, Fredros O. Okumu

“…Having been previously applied to An. gambiae, An. arabiensis and An. coluzzii, this demonstration on An. funestus underscores the potential of this low-cost, reagent-free technique for widespread use on all the major Afro-tropical malaria vectors. …”
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Impact of vegetable crop agriculture on anopheline agressivity and malaria transmission in urban and less urbanized settings of the South region of Cameroon by Patrick Ntonga Akono, Jean Arthur Mbida Mbida, Calvin Tonga, Philippe Belong, Odette Etoile Ngo Hondt, Gaëlle Tamdem Magne, Marie Florence Peka, Leopold Gustave Lehman

“…Five anopheline species were identified; An. gambiae, An. funestus s.s., An. moucheti s.s., An. hancocki and An. nili s.s. …”
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Laboratory and experimental hut evaluation of a long-lasting insecticide treated blanket for protection against mosquitoes by Jovin Kitau, Richard Oxborough, Angela Kaye, Vanessa Chen-Hussey, Evelyn Isaacs, Johnson Matowo, Harparkash Kaur, Stephen M Magesa, Franklin Mosha, Mark Rowland, James Logan

“…Methods Initial efficacy testing of a new candidate LLIB was carried out at LSHTM and KCMUCo, before and after washing, in cone and ball bioassays and arm-in-cage tests against pyrethroid susceptible Anopheles gambiae. A small scale field trial was conducted using veranda-trap experimental huts in northern Tanzania against wild An. arabiensis and Culex quinquefasciatus mosquitoes. …”
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Plasmodium malariae contributes to high levels of malaria transmission in a forest–savannah transition area in Cameroon by Daniel Nguiffo-Nguete, Francis Nongley Nkemngo, Cyrille Ndo, Jean-Pierre Agbor, Stravensky T. Boussougou-Sambe, Luc Salako Djogbénou, Francine Ntoumi, Ayôla A. Adegnika, Steffen Borrmann, Charles S. Wondji

“…Among the 757 Anopheles mosquitoes collected, 737 (97.35%) were An. funestus sensu stricto, 15 (1.9%) were An. gambiae and 5 (0.6%) were An. hancocki. The Plasmodium species recorded at the head/thorax level were P. falciparum and P. malariae, with a sporozoite infection rate of 8.4%; the highest sporozoite infection rate was recorded at Mibellon village (13.6%). …”
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Participatory development of practical, affordable, insecticide-treated mosquito proofing for a range of housing designs in rural southern Tanzania by Rogath Msoffe, Matilda Hewitt, John P. Masalu, Marcelina Finda, Deogratius R. Kavishe, Fredros O. Okumu, Emmanuel A. Mpolya, Emmanuel W. Kaindoa, Gerry F. Killeen

“…While PM did not augment household level protection by screens against either mosquito species (P = 0.676 and 0.831, respectively), 8 months after treatment it still caused 73% and 89% mortality among susceptible insectary-reared Anopheles gambiae following exposures of 3 and 30 min, respectively. …”
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Entomological longitudinal surveys in two contrasted eco-climatic settings in Cameroon reveal a high malaria transmission from Anopheles funestus associated with GSTe2 metabolic re... by Brice Natchema S. Fonkou, Magellan Tchouakui, Benjamin D. Menze, Leon M. J. Mugenzi, Derrick Fofie, Daniel Nguiffo-Nguete, Lucia Nkengazong, Jeannette Tombi, Charles S. Wondji

“…Results An. funestus s.l was the predominant malaria vector collected during the entomological survey in both sites (86.6% and 96.4% in Elende and Mibellon, respectively) followed by An. gambiae s.l (7.5% and 2.4%, respectively). Sporozoite infection rates were very high in both collection sites (8.7% and 11% in Elende and Mibellon, respectively). …”
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Sub-lethal exposure to chlorfenapyr reduces the probability of developing Plasmodium falciparum parasites in surviving Anopheles mosquitoes by Prisca A. Kweyamba, Lorenz M. Hofer, Ummi A. Kibondo, Rehema Y. Mwanga, Rajabu M. Sayi, Fatuma Matwewe, James W. Austin, Susanne Stutz, Sarah J. Moore, Pie Müller, Mgeni M. Tambwe

“…Pyrethroid-resistant Anopheles gambiae s.s. with L1014F and L1014S knockdown resistance alleles and expression levels of pyrethroid metabolisers CYP6P3, CYP6M2, CYP4G16 and CYP6P1 confirmed by quantitative reverse transcription polymerase chain reaction (RT-qPCR) prior to conducting experiments were exposed to untreated netting and netting treated with 200 mg/m3 chlorfenapyr for 8 h overnight and then fed on gametocytemic blood meals from naturally infected individuals. …”
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Efficacy of a ‘lethal house lure’ against Culex quinquefasciatus from Bouaké city, Côte d’Ivoire by Innocent Z. Tia, Antoine M. G. Barreaux, Welbeck A. Oumbouke, Alphonsine A. Koffi, Ludovic P. Ahoua Alou, Soromane Camara, Rosine Z. Wolie, Eleanore D. Sternberg, Amal Dahounto, Gregoire Y. Yapi, Matthew B. Thomas, Raphael N’Guessan

“…They attract nuisance host-seeking Cx. quinquefasciatus mosquitoes and are as effective in controlling them as they are against pyrethroid-resistant Anopheles gambiae, despite the high level of resistance Cx. quinquefasciatus have developed. …”
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Human and vector behaviors determine exposure to Anopheles in Namibia by Tabeth Mwema, Ophilia Lukubwe, Rosalia Joseph, Deodatus Maliti, Iitula Iitula, Stark Katokele, Petrina Uusiku, Dennis Walusimbi, Sheila Barasa Ogoma, Munya Tambo, Cara Smith Gueye, Yasmin A. Williams, Elodie Vajda, Allison Tatarsky, Seth J. Eiseb, Davis R. Mumbengegwi, Neil F. Lobo

“…Results Surveillance revealed the presence of the primary vectors Anopheles arabiensis, Anopheles gambiae sensu stricto (s.s.) and Anopheles funestus s.s., along with secondary vectors (Anopheles coustani sensu lato and Anopheles squamosus), with both indoor and outdoor biting behaviors based on the site. …”
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Life-history traits of a fluorescent Anopheles arabiensis genetic sexing strain introgressed into South African genomic background by Nonhlanhla L. Ntoyi, Thabo Mashatola, Jérémy Bouyer, Carina Kraupa, Hamidou Maiga, Wadaka Mamai, Nanwintoum S. Bimbile-Somda, Thomas Wallner, Danilo O. Carvalho, Givemore Munhenga, Hanano Yamada

“…This study is an assessment of an An. arabiensis (AY-2) strain carrying the full Y chromosome from Anopheles gambiae, including a transgenic red fluorescent marker, being introgressed into a South African genetic background as a potential tool for a reliable sexing system. …”
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Comparing the new Ifakara Ambient Chamber Test with WHO cone and tunnel tests for bioefficacy and non-inferiority testing of insecticide-treated nets by Dennis J. Massue, Lena M. Lorenz, Jason D. Moore, Watson S. Ntabaliba, Samuel Ackerman, Zawadi M. Mboma, William N. Kisinza, Emmanuel Mbuba, Selemani Mmbaga, John Bradley, Hans J. Overgaard, Sarah J. Moore

“…Methods Two experiments were conducted using pyrethroid-susceptible female adult Anopheles gambiae sensu stricto comparing bioefficacy of Olyset®, PermaNet® 2.0 and NetProtect® evaluated by I-ACT and WHO cone and tunnel tests. …”
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Affordable house designs to improve health in rural Africa: a field study from northeastern Tanzania by von Seidlein, L, Ikonomidis, K, Mshamu, S, Nkya, T, Mukaka, M, Pell, C, Lindsay, S, Deen, J, Kisinza, W, Knudsen, J

“…Mosquitoes of the Anopheles gambiae complex were identified to species using PCR. …”

High efficacy of chlorfenapyr-based net Interceptor® G2 against pyrethroid-resistant malaria vectors from Cameroon by Magellan Tchouakui, Riccado F. Thiomela, Elysee Nchoutpouen, Benjamin D. Menze, Cyrille Ndo, Dorothy Achu, Raymond N. Tabue, Flobert Njiokou, Ateba Joel, Charles S. Wondji

“…Methods The efficacy of these tools was firstly evaluated on Anopheles gambiae s.l. and Anopheles funestus s.l. from Gounougou, Mibellon, Mangoum, Nkolondom, and Elende using cone/tunnel assays. …”
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Mosquitoes, Lymphatic Filariasis, and Public Health: A Systematic Review of <i>Anopheles</i> and <i>Aedes</i> Surveillance Strategies by Arumugam Bhuvaneswari, Ananganallur Nagarajan Shriram, Kishan Hari K. Raju, Ashwani Kumar

“…Anopheles species are important vectors of filariasis in rural areas of Asia and Africa. The <i>Anopheles gambiae</i> complex, <i>Anopheles funestus</i>, and the <i>Anopheles punctulatus</i> group are the most important vectors of <i>W</i>. …”
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Burden of mosquito-borne diseases across rural versus urban areas in Cameroon between 2002 and 2021: prospective for community-oriented vector management approaches by Leo Dilane Alenou, Philippe Nwane, Lili Ranaise Mbakop, Michael Piameu, Wolfgang Ekoko, Stanislas Mandeng, Elisabeth Ngo Bikoy, Jean Claude Toto, Hugues Onguina, Josiane Etang

“…Of the articles reviewed for the 2012–2021 period, 56% reported the presence of Anopheles gambiae sensu lato in urban areas compared to 42% reported in 2002–2011. …”
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Influence of testing modality on bioefficacy for the evaluation of Interceptor® G2 mosquito nets to combat malaria mosquitoes in Tanzania by Ummi Abdul Kibondo, Olukayode G. Odufuwa, Saphina H. Ngonyani, Ahmadi B. Mpelepele, Issaya Matanilla, Hassan Ngonyani, Noel O. Makungwa, Antony P. Mseka, Kyeba Swai, Watson Ntabaliba, Susanne Stutz, James W. Austin, Sarah Jane Moore

“…Cone, tunnel and I-ACT bioassays used laboratory-reared metabolically resistant An. arabiensis and Cx. quinquefasciatus and pyrethroid susceptible Anopheles gambiae sensu stricto and Aedes aegypti. Results Against resistant strains, superiority of Interceptor® G2 over Interceptor® was observed in all “free-flying bioassays”. …”
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