High-level expression of endo-β-N-acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and its application for the deglycosylation of glycoproteins. by Fei Wang, Xiaojuan Wang, Xiaolan Yu, Ling Fu, Yunyun Liu, Lixin Ma, Chao Zhai

“…Its optimum pH and temperature were pH 5.5 and 37°C, respectively. Moreover, our study showed that the N-linked glycan side-chains of several recombinant proteins expressed in Pichia pastoris can be efficiently removed through either the co-fermentation of this recombinant strain with strains expressing substrates or by mixing the cell culture supernatants of the endo-β-N-acetylglucosaminidase H expressing strain with strains expressing substrates after fermentation. …”
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On-Line Preconcentration and Simultaneous Determination of Cu and Mn in Water Samples Using a Minicolumn Packed with Sisal Fiber by MIP OES by Javier Silva, Mariela Pistón

“…Optimal conditions for sample preparation were pH 5.5, preconcentration time was 90 s, and HCl 0.5 mol L⁻¹ was the eluent. …”
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Fermentation of Lulo Juice with <i>Lactobacillus reuteri</i> CECT 925. Properties and Effect of High Homogenization Pressures on Resistance to In Vitro Gastrointestinal Digestion by Leidy Indira Hinestroza-Córdoba, Ester Betoret, Lucía Seguí, Cristina Barrera, Noelia Betoret

“…Regarding the growth of <i>Lactobacillus reuteri</i> CECT 925, it was mainly affected by the pH of the medium, the rectified juice at pH 5.5 being the most appropriated one. The growth of <i>Lactobacillus reuteri</i> CECT 925 mainly increased the antiradical capacity of the juices. …”
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Evaluation of pilot scale cashew (Anacardium accidentale L.) oil extraction process in Laboratorio de Ingeniería Química, Universidad de Carabobo by Marjury Delgado, María Modroño Alonso, Viky Mujica, Maríam Ramírez, María del Carmen Rodríguez, Evelyn Yamal, Ingrid Velásquez

“…From oil characterization next properties were defined: saponification index 181.8 mg KOH/g, iodine 71.4 cg I/g, acidity 1.23 mg NaOH/g, peroxide 1.8 meq O2/g, refraction index 1.472 1, pH 5.5, relative density 0.89844, dynamic viscosity 71.6 cP, color 2.5 red/ 25 yellow. …”
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Production of Fructooligosaccharides Using a Commercial Heterologously Expressed <i>Aspergillus</i> sp. Fructosyltransferase by Klaudia Karkeszová, Milan Polakovič

“…The catalytic properties of Seqenzym^® FT, a fungal fructosyltransferase heterologously expressed in yeasts, were investigated at a temperature of 55 °C and pH 5.5. The initial rate measurements showed that the transfructosylation rate was only slightly inhibited by sucrose above the concentration of 1.5 M. …”
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PROTON-PUMPING AND NITROGEN METABOLISM IN RICE UNDER HUMID TROPIC CONDITIONS by Rodrigo Grazinoli Garrido, Fabiola de S.R.G. Garrido, Sonia Regina de Souza, Manlio Silvestre Fernandes

“…Rice plants were grown under 0.1 and 1.0 mM NH4+-N, in a Hoagland & Arnon nutrient solution, pH 5.5, up to 26 days after germination. The activity of PM H+-ATPases in the plasma membrane, vacuole VH+-ATPases, H+-PPases as well as GS, GOGAT, and GDH were determined. …”
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Choising the alternative conditions for the continuity of the work of the nitrate-selective electrode by هاجر ناصر, فاتن علاء الدين, هبه ناصر

“…The study of pH effect on the electrode response also showed that electrode works within the range (2-8)  of  pH and that the best response was at pH = 5.5 by using acetic acid-sodium acetate buffer. …”
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STUDY ON THE ABUNDANCE OF HETEROTROF BACTERIA ON LEAF LITTER MANGROVE IN COAST AREA SEDATI-SIDOARJO OF EAST JAVA by Nurmalitaa D., Nasukib, Rumayasab I.G., Susenob A.

“…The result of measurement of water quality parameter in this research ranged for pH 5.5-7.3, salinity 28-35, brightness 25-50 cm. Soil quality parameters are nitrite 0.143 mg/L, phosphate 0.160 mg/L, ammonia 2.05 mg/L, COD 80 mg/L and BOD 34 mg/L. …”
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Acidification of rat TRPV1 alters the kinetics of capsaicin responses by Faltynek Connie R, Han Ping, Jarvis Michael F, Neelands Torben R, Surowy Carol S

“…To investigate potential causes for this shift in capsaicin potency, the rates of current activation and deactivation of whole-cell currents were measured in individual cells exposed to treatments of pH 5.5, 1 μM capsaicin or in combination. Acidic pH was found to both increase the activation rate and decrease the deactivation rate of capsaicin-activated currents providing a possible mechanism for the enhanced potency of capsaicin under acidic conditions. …”
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TESTING OF THE EFFICIENCY OF SOME ENZYMATIC MIXTURES, CONCERNING THE CONVERSION OF SEVERAL LIGNOCELLULOSIC BIOMASS’ SOURSES, TO REDUCING SUGARS by RADIANA TAMBA-BEREHOIU, STEFANA JURCOANE, N. C. POPA, LILIANA BAGIU, ANA ROSU

“…The hydrolysis was performed at 550 C, for 20 h, at pH = 5,5. The best results were obtained by using MethaPlus enzyme. …”
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<i>Megasphaera elsdenii:</i> Its Role in Ruminant Nutrition and Its Potential Industrial Application for Organic Acid Biosynthesis by Luciano da Silva Cabral, Paul J. Weimer

“…The ruminal abundance of <i>M. elsdenii</i> usually increases in animals fed grain-based diets due to its ability to use lactate (the product of rapid ruminal sugar fermentation), especially at a low ruminal pH (<5.5). <i>M. elsdenii</i> has been proposed as a potential dietary probiotic to prevent ruminal acidosis in feedlot cattle and high-producing dairy cows. …”
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Chemical activation of sugar cane bagasse (Saccharum officinarum) on nickel (II) adsorption in industrial effluents by Sabrina Acevedo, Marinela Colina, Altamira Díaz, Nola Fernández, Elisabeth Behling, Julio Marín, Gilberto Colina

“…The PAC at 500°C can remove 85,66% of nickel with an Co of 50 mg/L at pH 5,5. The essays at pH 5 and 6 presented a nickel removal of 83,87 and 83,65% respectively. …”
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Improving Biodegradation of Clofibric Acid by <i>Trametes pubescens</i> through the Design of Experimental Tools by Claudia Veronica Ungureanu, Lidia Favier, Gabriela Elena Bahrim

“…After optimization, the capacity of the selected <i>Trametes pubescens</i> strain to degrade CLF was increased by cultivation in a liquid medium containing 3 g·L⁻¹ yeast extract, 15 g·L⁻¹ peptone, 5 g·L⁻¹ glucose and mineral salts, inoculated at 2% (<i>v/v</i>) vegetative inoculum and cultivated at pH 5.5, during 14 days at 25 °C and 135 rpm. In these optimized biotechnological conditions, the CLF biotransformation yield was 60%.…”
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The effect of low initial medium pH on in vitro white poplar growth by Kovačević Branislav, Miladinović Dragana, Katanić Marina, Tomović Zoran, Pekeč Saša

“…Generally, significantly higher values of fresh and dry shoot mass, shoot height and the longest root length were recorded on a medium with initial pH 3.0 then on a standard medium with pH 5.5.The implications of the obtained results for the improvement of in vitro propagation of white poplars are discussed. …”
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PENGARUH PERLAKUAN PENDAHULUAN PADA INKUBASI BIJI KAKAO KERING NON FERMENTASI DALAM BUFFER ASETAT TERHADAP SIFAT SENSORIS PASTA COKELAT GELAP by , Mukhammad iqbal Prawira Atmaja, , Prof. Dr.Ir. Haryadi, M.App.Sc.

“…Incubation was carried out in two stages, the first stage in acetic buffer medium pH 2.7, 600 mM for 24 hours, then a sec- ond stage in acetic buffer medium pH 5.5, 600 mM for 12 hours. While cocoa liq- uor roasting at of 120°C for 9 minutes. …”

The characterization of cellulase produced by buffalo rumen microbe:Determination of michaelis constants (Km) and maximum velocity (Vm) by Perpustakaan UGM, i-lib

“…Optimum activity of /3-glucosidase was obtained at temperature 50 °C and pH 5.0, while for CMCase was 50 °C and pH 5.5. On the other hand, optimum activity of FPase was obtained at temperature 45 °C and pH 6.0. …”

Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design by Alam, Md Zahangir, Zainan, Nur Hidayah, Alkhatib, Ma'an Fahmi Rashid

“…The validity of the model was verified and the optimum value of pH 5.5, agitation of 150rpm and 0.3% of KH2PO4 led to a maximum bioethanol production of 7.4 g/l. …”
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5-(1-propargylamino)-2'-deoxyuridine (UP): a novel thymidine analogue for generating DNA triplexes with increased stability. by Bijapur, J, Keppler, MD, Bergqvist, S, Brown, T, Fox, K

“…The intra-molecular triplex A6-L-T6-L-(UP)5T (L = two octanediol residues) shows a single UV-melting transition which is &gt;20 degrees higher than that of the parent triplex A6-L-T6-L-T6at pH 5.5. Although a single transition is observed at all pHs, the melting temperature (Tm) of the modified oligonucleotide decreases at higher pHs, consistent with the requirement for protonation of the amino group. …”

Optimisation of Remazol Brilliant Blue R dye decolourisation and laccase enzyme production by Marasmius cladophyllus using response surface methodology by Nur Adila Muradi, Ahmad Husaini, Azham Zulkharnain, Hairul Azman Roslan, Tay, Meng Guan

“…The fungus is found capable of decolourising 99% of the dye after 12 days of incubation in Glucose Minimal (GM) liquid media (pH 5.5) and laccase activity of 285 U/L was recorded. …”
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High-rate fermentative hydrogen production from palm oil mill effluent in an up-flow anaerobic sludge blanket-fixed film reactor by Mohammadi, P., Ibrahim, S., Annuar, M.S.M.

“…An up-flow anaerobic sludge blanket-fixed film (UASB-FF) reactor was initially inoculated with heat pretreated seed sludge as inoculum and operated as closed-loop fed-batch for five days (HRT = 24 h; 38 °C; pH 5.5). The reactor was continuously fed with fresh pre-settled POME in order to shorten the start-up period. …”