Cynodon dactylon methanol extract potentiates stress mitigation in response to acidic stress in Labeo calbasu fingerlings by THONGAM IBEMCHA CHANU, S DAM ROY, N K CHADHA, KIRAN DUBE, ARUN SHARMA, AJIT KESHAV CHAUDHURI, S KHOGEN SINGH

“…The possible antistress ability of C. dactylon methanol extract was assessed in terms of serum cortisol, serum glucose, serum cholesterol, serum alanine amino transferase (ALT), serum aspartate amino transferase (AST), lactate dehydrogenase (LDH), malate dehydrogenase (MDH), ATPase, superoxide dismutase (SOD), catalase (CAT) and Glutathione S transferase by exposing to acidic water with pH 5.5 for 24 h. The serum cortisol level was significantly higher in the acid stress group than that in without acid stress counterpart. …”
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Doxorubicin Conjugated γ-Globulin Functionalised Gold Nanoparticles: A pH-Responsive Bioinspired Nanoconjugate Approach for Advanced Chemotherapeutics by Gaurav Chauhan, Vianni Chopra, América García Alvarado, Jocelyn Alexandra Gómez Siono, Marc J. Madou, Sergio Omar Martinez-Chapa, Manish M. Kulkarni

“…In cell line studies, Dox-γG-AuNPs demonstrated a 10-fold higher cytotoxic potency compared to equivalent doxorubicin concentrations, with drug release favoured at pH 5.5 due to the γ-globulin corona’s inherent pH sensitivity. …”
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Therapeutic Drug Monitoring in Oncohematological Patients: A Fast and Accurate HPLC-UV Method for the Quantification of Nilotinib in Human Plasma and Its Clinical Application by Vanesa Escudero-Ortiz, Francisco José Rodríguez-Lucena, Gabriel Estan-Cerezo, Esther Mancheño-Maciá, Venancio Conesa-García, Ana García-Monsalve, Leticia Soriano-Irigaray, Andrés Navarro-Ruiz

“…After protein precipitation extraction with acetonitrile, nilotinib and rilpivirine were separated using isocratic elution on a Tracer Excel 120 ODS C18 column using a mobile phase consisting of a mixture of potassium dihydrogen phosphate-buffered solution (pH 5.5; 0.037 M)–methanol–acetonitrile (45:45:10, <i>v</i>/<i>v</i>/<i>v</i>), pumped at a flow rate of 1.7 mL·min⁻¹. …”
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Optimize the parameters for the synthesis by the ionic gelation technique, purification, and freeze-drying of chitosan-sodium tripolyphosphate nanoparticles for biomedical purposes by Stephany Celeste Gutiérrez-Ruíz, Hernán Cortes, Maykel González-Torres, Zainab M. Almarhoon, Eda Sönmez Gürer, Javad Sharifi-Rad, Gerardo Leyva-Gómez

“…Results Chitosan nanoparticles presented an average particle size of 172.8 ± 3.937 nm, PDI of 0.166 ± 0.008, and zeta potential of 25.00 ± 0.79 mV, at the concentration of 0.1% sodium tripolyphosphate and chitosan (pH 5.5), with a dripping time of 2 min at 500 rpm. …”
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Comparative Stability Study of Polysorbate 20 and Polysorbate 80 Related to Oxidative Degradation by Benedykt Kozuch, Johanna Weber, Julia Buske, Karsten Mäder, Patrick Garidel, Tim Diederichs

“…PS20 and PS80 in concentrations ranging from 0.1 mg⋅mL⁻¹ to raw material were stored at 5, 25, and 40 °C for 48 weeks in acetate buffer pH 5.5 and water, respectively. We observed a temperature-dependent oxidative degradation of the PSs with strong (40 °C), moderate (25 °C), and weak/no degradation (5 °C). …”
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Magnetoliposomes Based on Shape Anisotropic Calcium/Magnesium Ferrite Nanoparticles as Nanocarriers for Doxorubicin by Beatriz D. Cardoso, Ana Rita O. Rodrigues, Manuel Bañobre-López, Bernardo G. Almeida, Carlos O. Amorim, Vítor S. Amaral, Paulo J. G. Coutinho, Elisabete M. S. Castanheira

“…Finally, the drug release kinetic assays revealed a preferable DOX release at hyperthermia temperatures (42 °C) and acidic conditions (pH = 5.5), indicating them as promising controlled release nanocarriers by either internal (pH) and external (alternate magnetic field) stimuli in cancer therapy.…”
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Double-Coated Nanoparticle of Ribosome Inactivating Protein (RIP) from Mirabilis jalapa L. prepared from Chitosan-Sodium Tripolyphosphate and Alginate-Calcium Chloride: The New Str... by Miranda Amalia, Ismail Hilda, Martien Ronny, Hadiba Ciptasari Ummi, Kusniasari Ariyani, Ayu Arimurni Dewa, Dwi Pradipta Wahyudi S. Made, Sismindari

“…The result showed the optimum RMJCN-1 formula with of EE value of 57.10 ± 0.04 % was obtained by formulating 0.5 % w/v chitosan and 0.3 % w/v STPP in pH 5.5. The optimum RMJCN-2 was obtained by the combination of alginate 0.3 % w/v and CaCl2 0.1% w/v in the outer layer. …”
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Sleep Patterns During Pre-Competition Training Phase: A Comparison Between Male and Female Collegiate Swimmers by Xu A, Li X

“…This study aims to evaluate the sleep patterns of collegiate swimmers during a specific period (pre-competition training phase) based on the National Sleep Foundation’s recommendations and compares sleep differences between males and females.Patients and Methods: 15 swimmers (6 males and 9 females) completed the Athlete Sleep Screening Questionnaire (ASSQ) and wore actigraphy devices for 8 consecutive nights to record objective sleep patterns including bedtime, wake time, sleep onset latency, total sleep time, wake after sleep onset, and sleep efficiency.Results: The total sleep time of collegiate male (5.0± 0.4 h, 4.6 to 5.4h) and female (6.0± 0.7 h, 5.5 to 6.5h) swimmers was less than 7 hours per night, and male swimmers’ sleep efficiency (76.7± 8.9%, 67.4 to 86.0%) was lower than the 85% standard. …”
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Comparative evaluation of the therapeutic efficacy of rumen buffers and direct fed microbials (DFM) in sub-acute ruminal acidosis in dairy cows by S. Kumar Unni, C.G. Umesh, Magna Thomas, O.K. Sindhu, S. Ajithkumar

“…The condition is characterized by sustained and marked decrease in rumen liquor pH ≤5.5 for at least three hours of each day. Rumen buffers and direct fed microbials (DFM) are dietary supplements that had been used in the management of SARA. …”
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The potential use of supercritical carbon dioxide in sugarcane juice processing by Fernanda Cristina Pimenta, Talita Cristiane Krice Moraes, Gustavo Cesar Dacanal, Alessandra Lopes de Oliveira, Rodrigo Rodrigues Petrus

“…This study evaluated the combined effect of SC-CO2 and mild temperatures, primarily aiming for the reduction of endogenous microorganisms and enzymes in sugarcane juice (pH~5.5). Pressures (P) ranging from 74 to 351 bar, temperatures (T) between 33 and 67 °C, and holding times (t) between 20 and 70 min were tested in a central composite rotational design. …”
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Ứng dụng lysozyme để tạo chế phẩm bột oligosaccharide từ vỏ tôm thẻ chân trắng (Litopennaeus vannamei) by Võ Văn Song Toàn, Dương Thị Hương Giang, Lê Tấn Hòa, Nguyễn Thị Cẩm Giang, Kim Thị Thu Xương, Trương Thị Thanh Tuyền, Lê Ngọc Tuyết, Nguyễn Ngọc Phương Vy, Trần Nhân Dũng

“…Điều kiện thích hợp để thủy phân chitin huyền phù bằng lysozyme được xác định ở nhiệt độ 65ºC, pH 5,5 trong thời gian 12 giờ với hàm lượng cơ chất chitin huyền phù là 0,1 mg/mL. …”
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Kinetic and Regulatory Properties of <i>Yarrowia lipolytica</i> Aconitate Hydratase as a Model-Indicator of Cell Redox State under pH Stress by Tatyana I. Rakhmanova, Varvara Yu. Sekova, Natalya N. Gessler, Elena P. Isakova, Yulia I. Deryabina, Tatyana N. Popova, Yevgeniya I. Shurubor, Boris F. Krasnikov

“…A more pronounced effect of both GSH and GSSG was noted for the enzyme obtained from cells grown at pH 5.5. The data obtained provide new approaches to the use of <i>Y. lipolytica</i> as a model of eukaryotic cells demonstrating the development of a stress-induced pathology and to conducting a detailed analysis of enzymatic activity for its correction.…”
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Characterization and Evaluation of Bone-Derived Nanoparticles as a Novel pH-Responsive Carrier for Delivery of Doxorubicin into Breast Cancer Cells by Sheikh Tanzina Haque, Rowshan Ara Islam, Siew Hua Gan, Ezharul Hoque Chowdhury

“…<b>Results</b>: The pH-responsive drug release study demonstrated the prompt release of DOX from GBA through its disintegration in acidic pH (5.5–6.5), which mimics the pH of the endosomal and lysosomal compartments as well as the stability of GBA in physiological pH (pH 7.5). …”
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Separation, Purification and Characterization of Chitinase of the Endophytic Bacterium Bacillus thuringiensis Bt028 Isolated from Sour Orange by Qinqian XU, Guowei ZHU, Ziling ZHAO, Xueting TAO, Ziyuan LI, Zaibin HAO, Haiyun LI

“…The enzymatic characteristics study results showed that the optimum reaction temperature of the chitinase was 60 ℃ with good stability when temperature lower than 60 ℃; and the optimum reaction pH was 6.5 with good stability at pH5.5~7.5. Mg2+, Ca2+, Hg2+ and Co2+ had inhibitory effect on the enzyme activity, while Cu2+ and Fe3+ had a certain promotion effect. …”
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Association between Soil Acidity and Bacterial Wilt Occurrence in Potato Production in Ethiopia by Shiferaw Tafesse, Ciska Braam, Barbara van Mierlo, Berga Lemaga, Paul C. Struik

“…Currently, more than 40% of cultivated land in the country has a soil pH < 5.5. Recently, bacterial wilt (caused by <i>Ralstonia solanacearum</i>) has become a serious problem, reaching epidemic levels in some of the major potato growing districts in the country. …”
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Ultrasound-Assisted One-Pot Cloud Point Extraction for Iron Determination Using Natural Chelating Ligands from <i>Dipterocarpus intricatus</i> Dyer Fruit by Sam-ang Supharoek, Bordin Weerasuk, Watsaka Siriangkhawut, Kate Grudpan, Kraingkrai Ponhong

“…This method was based on the complexation of iron with an environmentally-friendly natural chelating agent extracted from <i>Dipterocarpus intricatus</i> Dyer fruit at pH 5.5 in the presence of Triton X-114. Reagent extraction, complexation, and preconcentration were performed simultaneously using ultrasound-assisted extraction at 45 °C. …”
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Aggregation of Cystatin C Changes Its Inhibitory Functions on Protease Activities and Amyloid β Fibril Formation by Abdullah Md. Sheikh, Yasuko Wada, Shatera Tabassum, Satoshi Inagaki, Shingo Mitaki, Shozo Yano, Atsushi Nagai

“…Cathepsin B activity assay demonstrated that aggregated CST3 inhibited the enzyme activity less efficiently at pH 5.5. At 7.4 pH, it lost the inhibitory properties almost completely. …”
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Determination of Zn²⁺ in Solid Pharmaceutical Dosage Forms by Means of Spectrophotometry in Micellar Media: Method Validation by Luca Liviu Rus, Anca Maria Juncan, Veronica Isabela Crăciun, Adina Frum, Simona-Codruța Hegheș, Anca Butuca, Carmen Maximiliana Dobrea, Adriana Aurelia Chis, Andrei Catalin Muntean, Andreea Loredana Vonica-Ţincu, Claudiu Morgovan

“…Zinc forms a ternary complex with Xylenol Orange and cetylpyridinium chloride in acetate buffer at pH = 5.5. The complex exhibits an absorption maximum at 560 nm. …”
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Novel biosynthesis of MnO NPs using Mycoendophyte: industrial bioprocessing strategies and scaling-up production with its evaluation as anti-phytopathogenic agents by Shahira H. EL-Moslamy, I. S. Yahia, H. Y. Zahran, Elbadawy A. Kamoun

“…The production improvements were achieved at pH 5.5, WBE (35%), and inoculum size (10%), which increased Xmax to twelve-folds (89.63 g/l); thereby, Pmax increased to eight-folds (82.93 g/l). …”
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NUTRIENT REGIME OF THE SOIL IN THE FIELD CROP ROTATION DIFFERENT DOSES FERTILIZER by Hospodarenko G.M.

“…The soil of the experimental site is black-ground podzolized heavy-sandy loam with humus content of 3.8%, nitrogen content of readily hydrolyzed compounds - low, mobile phosphorus and potassium compounds increased, pH 5.5. The experiment is simultaneously deployed on four fields with such alternation of crops: winter wheat, corn, spring barley, soy beans. …”
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